UniProtKB - Q6IBS0 (TWF2_HUMAN)
Protein
Twinfilin-2
Gene
TWF2
Organism
Homo sapiens (Human)
Status
Functioni
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia (By similarity).By similarity
GO - Molecular functioni
- actin filament binding Source: GO_Central
- actin monomer binding Source: BHF-UCL
- ATP binding Source: UniProtKB
- cadherin binding Source: BHF-UCL
- phosphatidylinositol-4,5-bisphosphate binding Source: BHF-UCL
- protein kinase C binding Source: BHF-UCL
- RNA binding Source: UniProtKB
GO - Biological processi
- actin filament depolymerization Source: GO_Central
- barbed-end actin filament capping Source: BHF-UCL
- cell projection organization Source: UniProtKB-KW
- cellular response to growth factor stimulus Source: BHF-UCL
- cellular response to retinoic acid Source: BHF-UCL
- negative regulation of actin filament polymerization Source: BHF-UCL
- positive regulation of axon extension Source: BHF-UCL
- positive regulation of lamellipodium assembly Source: BHF-UCL
- positive regulation of neuron projection development Source: BHF-UCL
- regulation of actin cytoskeleton organization Source: BHF-UCL
- regulation of lamellipodium assembly Source: GO_Central
- regulation of microvillus length Source: BHF-UCL
- sequestering of actin monomers Source: BHF-UCL
Keywordsi
Molecular function | Actin-binding |
Biological process | Cilium biogenesis/degradation |
Enzyme and pathway databases
PathwayCommonsi | Q6IBS0 |
Names & Taxonomyi
Protein namesi | Recommended name: Twinfilin-2Alternative name(s): A6-related protein Short name: hA6RP Protein tyrosine kinase 9-like Twinfilin-1-like protein |
Gene namesi | Name:TWF2 Synonyms:PTK9L ORF Names:MSTP011 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9621, TWF2 |
MIMi | 607433, gene |
neXtProti | NX_Q6IBS0 |
VEuPathDBi | HostDB:ENSG00000247596.8 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Other locations
- perinuclear region 1 Publication
- stereocilium By similarity
Note: Perinuclear and G-actin-rich cortical actin structure sublocalization.
Cytoskeleton
- actin filament Source: GO_Central
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Other locations
- cytoplasm Source: BHF-UCL
- filopodium Source: BHF-UCL
- growth cone Source: BHF-UCL
- lamellipodium Source: BHF-UCL
- myofibril Source: BHF-UCL
- perinuclear region of cytoplasm Source: BHF-UCL
- stereocilium Source: BHF-UCL
Keywords - Cellular componenti
Cell projection, Cytoplasm, CytoskeletonPathology & Biotechi
Organism-specific databases
DisGeNETi | 11344 |
OpenTargetsi | ENSG00000247596 |
PharmGKBi | PA162407429 |
Miscellaneous databases
Pharosi | Q6IBS0, Tbio |
Genetic variation databases
BioMutai | TWF2 |
DMDMi | 94730596 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000233136 | 2 – 349 | Twinfilin-2Add BLAST | 348 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | |
Modified residuei | 14 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 309 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 349 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
In vitro, phosphorylated by PRKCZ, CK2 and SRC.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q6IBS0 |
jPOSTi | Q6IBS0 |
MassIVEi | Q6IBS0 |
MaxQBi | Q6IBS0 |
PaxDbi | Q6IBS0 |
PeptideAtlasi | Q6IBS0 |
PRIDEi | Q6IBS0 |
ProteomicsDBi | 66370 |
2D gel databases
OGPi | Q9Y3F5 |
PTM databases
iPTMneti | Q6IBS0 |
PhosphoSitePlusi | Q6IBS0 |
SwissPalmi | Q6IBS0 |
Expressioni
Tissue specificityi
Ubiquitously expressed (at protein level).1 Publication
Gene expression databases
Bgeei | ENSG00000247596, Expressed in apex of heart and 231 other tissues |
ExpressionAtlasi | Q6IBS0, baseline and differential |
Genevisiblei | Q6IBS0, HS |
Organism-specific databases
HPAi | ENSG00000247596, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF1 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated.
Interacts with MYO7A (By similarity).
By similarityBinary interactionsi
Q6IBS0
With | #Exp. | IntAct |
---|---|---|
TERF2IP [Q9NYB0] | 2 | EBI-722204,EBI-750109 |
GO - Molecular functioni
- actin filament binding Source: GO_Central
- actin monomer binding Source: BHF-UCL
- cadherin binding Source: BHF-UCL
- protein kinase C binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 116472, 70 interactors |
IntActi | Q6IBS0, 30 interactors |
MINTi | Q6IBS0 |
STRINGi | 9606.ENSP00000303908 |
Miscellaneous databases
RNActi | Q6IBS0, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q6IBS0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q6IBS0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 139 | ADF-H 1PROSITE-ProRule annotationAdd BLAST | 136 | |
Domaini | 177 – 313 | ADF-H 2PROSITE-ProRule annotationAdd BLAST | 137 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1747, Eukaryota |
GeneTreei | ENSGT00530000063868 |
HOGENOMi | CLU_031995_1_0_1 |
InParanoidi | Q6IBS0 |
OMAi | EMFGTTE |
OrthoDBi | 1578109at2759 |
PhylomeDBi | Q6IBS0 |
TreeFami | TF352598 |
Family and domain databases
Gene3Di | 3.40.20.10, 2 hits |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR028458, Twinfilin |
PANTHERi | PTHR13759, PTHR13759, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 2 hits |
SMARTi | View protein in SMART SM00102, ADF, 2 hits |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q6IBS0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAHQTGIHAT EELKEFFAKA RAGSVRLIKV VIEDEQLVLG ASQEPVGRWD
60 70 80 90 100
QDYDRAVLPL LDAQQPCYLL YRLDSQNAQG FEWLFLAWSP DNSPVRLKML
110 120 130 140 150
YAATRATVKK EFGGGHIKDE LFGTVKDDLS FAGYQKHLSS CAAPAPLTSA
160 170 180 190 200
ERELQQIRIN EVKTEISVES KHQTLQGLAF PLQPEAQRAL QQLKQKMVNY
210 220 230 240 250
IQMKLDLERE TIELVHTEPT DVAQLPSRVP RDAARYHFFL YKHTHEGDPL
260 270 280 290 300
ESVVFIYSMP GYKCSIKERM LYSSCKSRLL DSVEQDFHLE IAKKIEIGDG
310 320 330 340
AELTAEFLYD EVHPKQHAFK QAFAKPKGPG GKRGHKRLIR GPGENGDDS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD6RG15 | D6RG15_HUMAN | Twinfilin-2 | TWF2 | 254 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 110 | K → R in CAG33013 (Ref. 4) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_042407 | 72 | R → C1 PublicationCorresponds to variant dbSNP:rs35114109Ensembl. | 1 | |
Natural variantiVAR_042408 | 76 | Q → R1 PublicationCorresponds to variant dbSNP:rs35711542Ensembl. | 1 | |
Natural variantiVAR_042409 | 103 | A → T in a lung neuroendocrine carcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs867679383Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y17169 mRNA Translation: CAB38055.1 AL136773 mRNA Translation: CAB66707.1 AF109365 mRNA Translation: AAQ13513.1 CR456732 mRNA Translation: CAG33013.1 CR533520 mRNA Translation: CAG38551.1 BC000327 mRNA Translation: AAH00327.1 BC003161 mRNA Translation: AAH03161.1 BC016452 mRNA Translation: AAH16452.1 |
CCDSi | CCDS2849.1 |
PIRi | T46362 |
RefSeqi | NP_009215.1, NM_007284.3 |
Genome annotation databases
Ensembli | ENST00000305533; ENSP00000303908; ENSG00000247596 |
GeneIDi | 11344 |
KEGGi | hsa:11344 |
UCSCi | uc003ddd.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight Molecular embrace - Issue 73 of August 2006 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y17169 mRNA Translation: CAB38055.1 AL136773 mRNA Translation: CAB66707.1 AF109365 mRNA Translation: AAQ13513.1 CR456732 mRNA Translation: CAG33013.1 CR533520 mRNA Translation: CAG38551.1 BC000327 mRNA Translation: AAH00327.1 BC003161 mRNA Translation: AAH03161.1 BC016452 mRNA Translation: AAH16452.1 |
CCDSi | CCDS2849.1 |
PIRi | T46362 |
RefSeqi | NP_009215.1, NM_007284.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2VAC | X-ray | 1.70 | A | 6-137 | [»] | |
2W0I | X-ray | 1.80 | A | 181-313 | [»] | |
SMRi | Q6IBS0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116472, 70 interactors |
IntActi | Q6IBS0, 30 interactors |
MINTi | Q6IBS0 |
STRINGi | 9606.ENSP00000303908 |
PTM databases
iPTMneti | Q6IBS0 |
PhosphoSitePlusi | Q6IBS0 |
SwissPalmi | Q6IBS0 |
Genetic variation databases
BioMutai | TWF2 |
DMDMi | 94730596 |
2D gel databases
OGPi | Q9Y3F5 |
Proteomic databases
EPDi | Q6IBS0 |
jPOSTi | Q6IBS0 |
MassIVEi | Q6IBS0 |
MaxQBi | Q6IBS0 |
PaxDbi | Q6IBS0 |
PeptideAtlasi | Q6IBS0 |
PRIDEi | Q6IBS0 |
ProteomicsDBi | 66370 |
Protocols and materials databases
Antibodypediai | 46152, 230 antibodies |
DNASUi | 11344 |
Genome annotation databases
Ensembli | ENST00000305533; ENSP00000303908; ENSG00000247596 |
GeneIDi | 11344 |
KEGGi | hsa:11344 |
UCSCi | uc003ddd.4, human |
Organism-specific databases
CTDi | 11344 |
DisGeNETi | 11344 |
GeneCardsi | TWF2 |
HGNCi | HGNC:9621, TWF2 |
HPAi | ENSG00000247596, Tissue enhanced (skeletal) |
MIMi | 607433, gene |
neXtProti | NX_Q6IBS0 |
OpenTargetsi | ENSG00000247596 |
PharmGKBi | PA162407429 |
VEuPathDBi | HostDB:ENSG00000247596.8 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1747, Eukaryota |
GeneTreei | ENSGT00530000063868 |
HOGENOMi | CLU_031995_1_0_1 |
InParanoidi | Q6IBS0 |
OMAi | EMFGTTE |
OrthoDBi | 1578109at2759 |
PhylomeDBi | Q6IBS0 |
TreeFami | TF352598 |
Enzyme and pathway databases
PathwayCommonsi | Q6IBS0 |
Miscellaneous databases
BioGRID-ORCSi | 11344, 8 hits in 875 CRISPR screens |
ChiTaRSi | TWF2, human |
EvolutionaryTracei | Q6IBS0 |
GeneWikii | TWF2 |
GenomeRNAii | 11344 |
Pharosi | Q6IBS0, Tbio |
PROi | PR:Q6IBS0 |
RNActi | Q6IBS0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000247596, Expressed in apex of heart and 231 other tissues |
ExpressionAtlasi | Q6IBS0, baseline and differential |
Genevisiblei | Q6IBS0, HS |
Family and domain databases
Gene3Di | 3.40.20.10, 2 hits |
InterProi | View protein in InterPro IPR002108, ADF-H IPR029006, ADF-H/Gelsolin-like_dom_sf IPR028458, Twinfilin |
PANTHERi | PTHR13759, PTHR13759, 1 hit |
Pfami | View protein in Pfam PF00241, Cofilin_ADF, 2 hits |
SMARTi | View protein in SMART SM00102, ADF, 2 hits |
PROSITEi | View protein in PROSITE PS51263, ADF_H, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TWF2_HUMAN | |
Accessioni | Q6IBS0Primary (citable) accession number: Q6IBS0 Secondary accession number(s): Q9Y3F5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2006 |
Last sequence update: | May 2, 2006 | |
Last modified: | February 10, 2021 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries - Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants