UniProtKB - Q6HD43 (IXTPA_BACHK)
Protein
dITP/XTP pyrophosphatase
Gene
BT9727_4217
Organism
Bacillus thuringiensis subsp. konkukian (strain 97-27)
Status
Functioni
Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation
Catalytic activityi
Cofactori
Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 44 | MagnesiumUniRule annotation | 1 | |
Active sitei | 73 | Proton acceptorUniRule annotation | 1 | |
Metal bindingi | 73 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 74 | Substrate; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 181 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- nucleoside-triphosphatase activity Source: InterPro
- nucleoside-triphosphate diphosphatase activity Source: UniProtKB-UniRule
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- nucleotide metabolic process Source: UniProtKB-KW
- purine nucleoside triphosphate catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Hydrolase |
Biological process | Nucleotide metabolism |
Ligand | Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BTHU281309:G1G14-4289-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)Alternative name(s): Non-canonical purine NTP pyrophosphataseUniRule annotation Non-standard purine NTP pyrophosphataseUniRule annotation Nucleoside-triphosphate diphosphataseUniRule annotation Nucleoside-triphosphate pyrophosphataseUniRule annotation Short name: NTPaseUniRule annotation |
Gene namesi | Ordered Locus Names:BT9727_4217 |
Organismi | Bacillus thuringiensis subsp. konkukian (strain 97-27) |
Taxonomic identifieri | 281309 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000178126 | 1 – 205 | dITP/XTP pyrophosphataseAdd BLAST | 205 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 11 – 16 | Substrate bindingUniRule annotation | 6 | |
Regioni | 158 – 161 | Substrate bindingUniRule annotation | 4 | |
Regioni | 186 – 187 | Substrate bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the HAM1 NTPase family.UniRule annotation
Phylogenomic databases
HOGENOMi | HOG000293319 |
KOi | K02428 |
OMAi | YDPIFQP |
Family and domain databases
CDDi | cd00515 HAM1, 1 hit |
Gene3Di | 3.90.950.10, 1 hit |
HAMAPi | MF_01405 Non_canon_purine_NTPase, 1 hit |
InterProi | View protein in InterPro IPR020922 dITP/XTP_pyrophosphatase IPR002637 Ham1p-like IPR029001 ITPase-like_fam |
PANTHERi | PTHR11067 PTHR11067, 1 hit |
Pfami | View protein in Pfam PF01725 Ham1p_like, 1 hit |
SUPFAMi | SSF52972 SSF52972, 1 hit |
TIGRFAMsi | TIGR00042 TIGR00042, 1 hit |
i Sequence
Sequence statusi: Complete.
Q6HD43-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MENMKQVVVA TKNMGKVREF AELFERFDLE VKSLHDFPHI EEVEETGETF
60 70 80 90 100
EENAILKADS LSRQLNAIVI ADDSGLIVDA LNGKPGVYSA RFAGEPKDDQ
110 120 130 140 150
ANIDKVLQEL NEVAFEKRKA RFYCALAVAF PEGDKKPVIV NGTCEGFILE
160 170 180 190 200
QRRGENGFGY DPIFYVEEYK KAMAELSSDE KNAISHRGRA LRKLEEKIPE
WFLGE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017355 Genomic DNA Translation: AAT63711.1 |
RefSeqi | WP_002003580.1, NC_005957.1 YP_038533.1, NC_005957.1 |
Genome annotation databases
EnsemblBacteriai | AAT63711; AAT63711; BT9727_4217 |
GeneIDi | 2855522 |
KEGGi | btk:BT9727_4217 |
PATRICi | fig|281309.8.peg.4495 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE017355 Genomic DNA Translation: AAT63711.1 |
RefSeqi | WP_002003580.1, NC_005957.1 YP_038533.1, NC_005957.1 |
3D structure databases
SMRi | Q6HD43 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | AAT63711; AAT63711; BT9727_4217 |
GeneIDi | 2855522 |
KEGGi | btk:BT9727_4217 |
PATRICi | fig|281309.8.peg.4495 |
Phylogenomic databases
HOGENOMi | HOG000293319 |
KOi | K02428 |
OMAi | YDPIFQP |
Enzyme and pathway databases
BioCyci | BTHU281309:G1G14-4289-MONOMER |
Family and domain databases
CDDi | cd00515 HAM1, 1 hit |
Gene3Di | 3.90.950.10, 1 hit |
HAMAPi | MF_01405 Non_canon_purine_NTPase, 1 hit |
InterProi | View protein in InterPro IPR020922 dITP/XTP_pyrophosphatase IPR002637 Ham1p-like IPR029001 ITPase-like_fam |
PANTHERi | PTHR11067 PTHR11067, 1 hit |
Pfami | View protein in Pfam PF01725 Ham1p_like, 1 hit |
SUPFAMi | SSF52972 SSF52972, 1 hit |
TIGRFAMsi | TIGR00042 TIGR00042, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IXTPA_BACHK | |
Accessioni | Q6HD43Primary (citable) accession number: Q6HD43 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | July 19, 2004 | |
Last modified: | September 18, 2019 | |
This is version 82 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteomeDocuments
- SIMILARITY comments
Index of protein domains and families