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Protein

Bifunctional nitrilase/nitrile hydratase NIT4

Gene

NIT4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Highly specific for beta-cyano-L-alanine (Ala(CN)). Low activity with 3-phenylpropionitrile (PPN). Not associated with auxin production but may be involved in cyanide detoxification (By similarity).By similarity

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.PROSITE-ProRule annotation
3-cyano-L-alanine + 2 H2O = L-aspartate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71Proton acceptorPROSITE-ProRule annotation1
Active sitei162Proton donorPROSITE-ProRule annotation1
Active sitei196NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional nitrilase/nitrile hydratase NIT4 (EC:3.5.5.1, EC:3.5.5.4)
Short name:
OsNIT4
Alternative name(s):
Cyanoalanine nitrilase
Nitrilase 4
Gene namesi
Name:NIT4
Ordered Locus Names:Os02g0635200, LOC_Os02g42350
ORF Names:OJ1626_B09.8, OsJ_007404
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003525131 – 362Bifunctional nitrilase/nitrile hydratase NIT4Add BLAST362

Proteomic databases

PaxDbiQ6H849

Expressioni

Gene expression databases

ExpressionAtlasiQ6H849 differential
GenevisibleiQ6H849 OS

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os02g42350.1

Structurei

3D structure databases

ProteinModelPortaliQ6H849
SMRiQ6H849
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 307CN hydrolasePROSITE-ProRule annotationAdd BLAST277

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi255 – 258Poly-Pro4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0805 Eukaryota
COG0388 LUCA
HOGENOMiHOG000256365
InParanoidiQ6H849
KOiK13035
OMAiTMRHIAL
OrthoDBiEOG09360CLO

Family and domain databases

Gene3Di3.60.110.10, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR000132 Nitrilase/CN_hydratase_CS
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit
PS00920 NITRIL_CHT_1, 1 hit
PS00921 NITRIL_CHT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q6H849-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMVPSGSGG GPPVIAEVEM NGGADSGAAT VRATVVQAST VFYDTPATLD
60 70 80 90 100
KAERLIEEAA GYGSQLVVFP EAFVGGYPRG STFGFGANIS IGNPKDKGKE
110 120 130 140 150
EFRKYHAAAI EVPGPEVTRL AAMAGKYKVF LVMGVIEREG YTLYCSVLFF
160 170 180 190 200
DPLGRYLGKH RKLMPTALER IIWGFGDGST IPVYDTPLGK IGALICWENK
210 220 230 240 250
MPLLRTALYG KGIEIYCAPT ADSRQVWQAS MTHIALEGGC FVLSANQFCR
260 270 280 290 300
RKDYPPPPEY VFSGLGEEPS PDTVVCPGGS VIISPSGEVL AGPNYEGEAL
310 320 330 340 350
ITADLDLGEI VRAKFDFDVV GHYARPEVLS LVVNDQPHLP VSFTSAAEKT
360
TAAKSDSTAK PY
Length:362
Mass (Da):38,676
Last modified:July 19, 2004 - v1
Checksum:i5D3CA71FFD95FDE0
GO

Sequence cautioni

The sequence EAZ23921 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25D → T in BAA77679 (Ref. 1) Curated1
Sequence conflicti263S → T in BAA77679 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027054 mRNA Translation: BAA77679.1
AP004069 Genomic DNA Translation: BAD25100.1
AP008208 Genomic DNA Translation: BAF09432.1
AP014958 Genomic DNA Translation: BAS79936.1
CM000139 Genomic DNA Translation: EAZ23921.1 Sequence problems.
AK104033 mRNA Translation: BAG96382.1
AK121244 mRNA Translation: BAH00392.1
PIRiT52266
RefSeqiXP_015622782.1, XM_015767296.1
UniGeneiOs.4158

Genome annotation databases

EnsemblPlantsiOs02t0635200-01; Os02t0635200-01; Os02g0635200
GeneIDi4330077
GrameneiOs02t0635200-01; Os02t0635200-01; Os02g0635200
KEGGiosa:4330077

Similar proteinsi

Entry informationi

Entry nameiNRL4_ORYSJ
AccessioniPrimary (citable) accession number: Q6H849
Secondary accession number(s): A3A9D2, B7EVI5, Q9SXX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: July 19, 2004
Last modified: May 23, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

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