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Protein

N-fatty-acyl-amino acid synthase/hydrolase PM20D1

Gene

PM20D1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. Thereby, this secreted protein may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.1 Publication

Catalytic activityi

A N-fatty-acyl-L-amino acid + H2O = a fatty acid + an amino acid.By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi125Zinc 2By similarity1
Active sitei127By similarity1
Metal bindingi157Zinc 1By similarity1
Metal bindingi157Zinc 2By similarity1
Active sitei191Proton acceptorBy similarity1
Metal bindingi192Zinc 1By similarity1
Metal bindingi217Zinc 2By similarity1
Metal bindingi464Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM20.011

Chemistry databases

SwissLipidsiSLP:000001665

Names & Taxonomyi

Protein namesi
Recommended name:
N-fatty-acyl-amino acid synthase/hydrolase PM20D11 Publication (EC:3.5.1.-1 Publication, EC:4.3.-.-1 Publication)
Alternative name(s):
Peptidase M20 domain-containing protein 1Imported
Gene namesi
Name:PM20D1Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000162877.12
HGNCiHGNC:26518 PM20D1
MIMi617124 gene
neXtProtiNX_Q6GTS8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi148811
OpenTargetsiENSG00000162877
PharmGKBiPA162399772

Polymorphism and mutation databases

BioMutaiPM20D1
DMDMi317373406

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000032192826 – 502N-fatty-acyl-amino acid synthase/hydrolase PM20D1Add BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi252N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6GTS8
PeptideAtlasiQ6GTS8
PRIDEiQ6GTS8
ProteomicsDBi66320
66321 [Q6GTS8-2]

PTM databases

iPTMnetiQ6GTS8
PhosphoSitePlusiQ6GTS8

Expressioni

Gene expression databases

BgeeiENSG00000162877 Expressed in 87 organ(s), highest expression level in mammalian vulva
CleanExiHS_PM20D1
GenevisibleiQ6GTS8 HS

Interactioni

Protein-protein interaction databases

BioGridi127171, 2 interactors
STRINGi9606.ENSP00000356104

Structurei

3D structure databases

ProteinModelPortaliQ6GTS8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2275 Eukaryota
COG0624 LUCA
GeneTreeiENSGT00880000137960
HOGENOMiHOG000245216
HOVERGENiHBG059923
InParanoidiQ6GTS8
KOiK13049
OMAiPGICIGN
OrthoDBiEOG091G0BP2
PhylomeDBiQ6GTS8
TreeFamiTF328688

Family and domain databases

InterProiView protein in InterPro
IPR036264 Bact_exopeptidase_dim_dom
IPR002933 Peptidase_M20
IPR011650 Peptidase_M20_dimer
PfamiView protein in Pfam
PF07687 M20_dimer, 1 hit
PF01546 Peptidase_M20, 1 hit
SUPFAMiSSF55031 SSF55031, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q6GTS8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAQRCVCVLA LVAMLLLVFP TVSRSMGPRS GEHQRASRIP SQFSKEERVA
60 70 80 90 100
MKEALKGAIQ IPTVTFSSEK SNTTALAEFG KYIHKVFPTV VSTSFIQHEV
110 120 130 140 150
VEEYSHLFTI QGSDPSLQPY LLMAHFDVVP APEEGWEVPP FSGLERDGII
160 170 180 190 200
YGRGTLDDKN SVMALLQALE LLLIRKYIPR RSFFISLGHD EESSGTGAQR
210 220 230 240 250
ISALLQSRGV QLAFIVDEGG FILDDFIPNF KKPIALIAVS EKGSMNLMLQ
260 270 280 290 300
VNMTSGHSSA PPKETSIGIL AAAVSRLEQT PMPIIFGSGT VVTVLQQLAN
310 320 330 340 350
EFPFPVNIIL SNPWLFEPLI SRFMERNPLT NAIIRTTTAL TIFKAGVKFN
360 370 380 390 400
VIPPVAQATV NFRIHPGQTV QEVLELTKNI VADNRVQFHV LSAFDPLPVS
410 420 430 440 450
PSDDKALGYQ LLRQTVQSVF PEVNITAPVT SIGNTDSRFF TNLTTGIYRF
460 470 480 490 500
YPIYIQPEDF KRIHGVNEKI SVQAYETQVK FIFELIQNAD TDQEPVSHLH

KL
Length:502
Mass (Da):55,741
Last modified:January 11, 2011 - v3
Checksum:i0CC2C45E1A7ADEA8
GO
Isoform 2 (identifier: Q6GTS8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     302-361: FPFPVNIILS...IPPVAQATVN → VYGEKSLNQC...GPRTHEEHCG
     362-502: Missing.

Note: No experimental confirmation available.
Show »
Length:361
Mass (Da):39,544
Checksum:i74340982D6CE39A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84H → R in AAH39170 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03938033H → Y1 PublicationCorresponds to variant dbSNP:rs11540014Ensembl.1
Natural variantiVAR_039381149I → V2 PublicationsCorresponds to variant dbSNP:rs1891460Ensembl.1
Natural variantiVAR_039382153R → W2 PublicationsCorresponds to variant dbSNP:rs1104899Ensembl.1
Natural variantiVAR_039383237I → T1 PublicationCorresponds to variant dbSNP:rs7518979Ensembl.1
Natural variantiVAR_039384258S → C. Corresponds to variant dbSNP:rs11581214Ensembl.1
Natural variantiVAR_039385346G → R1 PublicationCorresponds to variant dbSNP:rs11240573Ensembl.1
Natural variantiVAR_039386380I → T1 PublicationCorresponds to variant dbSNP:rs1361754Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_031826302 – 361FPFPV…QATVN → VYGEKSLNQCNNQDHHGTHH IQSRGQVQCHPPSGPGHSQL PDSPWTDSPRGPRTHEEHCG in isoform 2. 1 PublicationAdd BLAST60
Alternative sequenceiVSP_031827362 – 502Missing in isoform 2. 1 PublicationAdd BLAST141

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057131 mRNA Translation: BAB71368.1
AK290786 mRNA Translation: BAF83475.1
AC119673 Genomic DNA No translation available.
BC039170 mRNA Translation: AAH39170.1
BC063477 mRNA Translation: AAH63477.1
CCDSiCCDS1460.1 [Q6GTS8-1]
RefSeqiNP_689704.4, NM_152491.4 [Q6GTS8-1]
UniGeneiHs.177744

Genome annotation databases

EnsembliENST00000367136; ENSP00000356104; ENSG00000162877 [Q6GTS8-1]
GeneIDi148811
KEGGihsa:148811
UCSCiuc001hdj.4 human [Q6GTS8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

A touch of warmth - Issue 195 of September 2017

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057131 mRNA Translation: BAB71368.1
AK290786 mRNA Translation: BAF83475.1
AC119673 Genomic DNA No translation available.
BC039170 mRNA Translation: AAH39170.1
BC063477 mRNA Translation: AAH63477.1
CCDSiCCDS1460.1 [Q6GTS8-1]
RefSeqiNP_689704.4, NM_152491.4 [Q6GTS8-1]
UniGeneiHs.177744

3D structure databases

ProteinModelPortaliQ6GTS8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127171, 2 interactors
STRINGi9606.ENSP00000356104

Chemistry databases

SwissLipidsiSLP:000001665

Protein family/group databases

MEROPSiM20.011

PTM databases

iPTMnetiQ6GTS8
PhosphoSitePlusiQ6GTS8

Polymorphism and mutation databases

BioMutaiPM20D1
DMDMi317373406

Proteomic databases

PaxDbiQ6GTS8
PeptideAtlasiQ6GTS8
PRIDEiQ6GTS8
ProteomicsDBi66320
66321 [Q6GTS8-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367136; ENSP00000356104; ENSG00000162877 [Q6GTS8-1]
GeneIDi148811
KEGGihsa:148811
UCSCiuc001hdj.4 human [Q6GTS8-1]

Organism-specific databases

CTDi148811
DisGeNETi148811
EuPathDBiHostDB:ENSG00000162877.12
GeneCardsiPM20D1
HGNCiHGNC:26518 PM20D1
MIMi617124 gene
neXtProtiNX_Q6GTS8
OpenTargetsiENSG00000162877
PharmGKBiPA162399772
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2275 Eukaryota
COG0624 LUCA
GeneTreeiENSGT00880000137960
HOGENOMiHOG000245216
HOVERGENiHBG059923
InParanoidiQ6GTS8
KOiK13049
OMAiPGICIGN
OrthoDBiEOG091G0BP2
PhylomeDBiQ6GTS8
TreeFamiTF328688

Miscellaneous databases

GenomeRNAii148811
PROiPR:Q6GTS8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162877 Expressed in 87 organ(s), highest expression level in mammalian vulva
CleanExiHS_PM20D1
GenevisibleiQ6GTS8 HS

Family and domain databases

InterProiView protein in InterPro
IPR036264 Bact_exopeptidase_dim_dom
IPR002933 Peptidase_M20
IPR011650 Peptidase_M20_dimer
PfamiView protein in Pfam
PF07687 M20_dimer, 1 hit
PF01546 Peptidase_M20, 1 hit
SUPFAMiSSF55031 SSF55031, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiP20D1_HUMAN
AccessioniPrimary (citable) accession number: Q6GTS8
Secondary accession number(s): Q6P4E3, Q96DM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 11, 2011
Last modified: November 7, 2018
This is version 116 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  7. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
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