UniProtKB - Q6GQT1 (A2MG_MOUSE)
Alpha-2-macroglobulin-P
A2m
Functioni
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity).
By similarityGO - Molecular functioni
- brain-derived neurotrophic factor binding Source: MGI
- calcium-dependent protein binding Source: MGI
- endopeptidase inhibitor activity Source: MGI
- enzyme binding Source: MGI
- growth factor binding Source: MGI
- identical protein binding Source: MGI
- interleukin-1 binding Source: MGI
- interleukin-8 binding Source: MGI
- nerve growth factor binding Source: MGI
- peptidase inhibitor activity Source: MGI
- protease binding Source: MGI
- serine-type endopeptidase inhibitor activity Source: MGI
- signaling receptor binding Source: MGI
- tumor necrosis factor binding Source: MGI
GO - Biological processi
- acute inflammatory response to antigenic stimulus Source: Ensembl
- acute-phase response Source: Ensembl
- embryonic liver development Source: Ensembl
- female pregnancy Source: UniProtKB-KW
- luteinization Source: Ensembl
- negative regulation of complement activation, lectin pathway Source: MGI
- negative regulation of endopeptidase activity Source: MGI
- response to carbon dioxide Source: Ensembl
- response to glucocorticoid Source: Ensembl
- response to nutrient Source: Ensembl
- response to prostaglandin E Source: Ensembl
- stem cell differentiation Source: MGI
Keywordsi
Molecular function | Protease inhibitor, Serine protease inhibitor |
Biological process | Pregnancy |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-140837, Intrinsic Pathway of Fibrin Clot Formation R-MMU-1474228, Degradation of the extracellular matrix R-MMU-8963896, HDL assembly |
Protein family/group databases
MEROPSi | I39.004 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-2-macroglobulin-PAlternative name(s): Alpha-2-macroglobulin |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2449119, A2m |
VEuPathDBi | HostDB:ENSMUSG00000030111 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: MGI
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 32 | Sequence analysisAdd BLAST | 32 | |
ChainiPRO_0000271402 | 33 – 1474 | Alpha-2-macroglobulin-PSequence analysisAdd BLAST | 1442 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 55 ↔ 93 | By similarity | ||
Glycosylationi | 62 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 77 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 253 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 257 ↔ 305 | By similarity | ||
Disulfide bondi | 275 ↔ 293 | By similarity | ||
Disulfide bondi | 284 | Interchain (with C-437)By similarity | ||
Glycosylationi | 402 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 437 | Interchain (with C-284)By similarity | ||
Disulfide bondi | 476 ↔ 569 | By similarity | ||
Disulfide bondi | 601 ↔ 771 | By similarity | ||
Disulfide bondi | 650 ↔ 697 | By similarity | ||
Glycosylationi | 654 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 774 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 821 ↔ 849 | By similarity | ||
Disulfide bondi | 847 ↔ 883 | By similarity | ||
Glycosylationi | 869 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 921 ↔ 1321 | By similarity | ||
Cross-linki | 972 ↔ 975 | Isoglutamyl cysteine thioester (Cys-Gln)By similarity | ||
Glycosylationi | 991 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 1079 ↔ 1127 | By similarity | ||
Disulfide bondi | 1352 ↔ 1467 | By similarity | ||
Glycosylationi | 1366 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Thioester bondProteomic databases
CPTACi | non-CPTAC-3311 non-CPTAC-3384 |
jPOSTi | Q6GQT1 |
MaxQBi | Q6GQT1 |
PaxDbi | Q6GQT1 |
PeptideAtlasi | Q6GQT1 |
PRIDEi | Q6GQT1 |
ProteomicsDBi | 286006 |
PTM databases
GlyGeni | Q6GQT1, 9 sites |
iPTMneti | Q6GQT1 |
PhosphoSitePlusi | Q6GQT1 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000030111, Expressed in decidua and 209 other tissues |
ExpressionAtlasi | Q6GQT1, baseline and differential |
Genevisiblei | Q6GQT1, MM |
Interactioni
Subunit structurei
Homotetramer; disulfide-linked.
By similarityGO - Molecular functioni
- brain-derived neurotrophic factor binding Source: MGI
- calcium-dependent protein binding Source: MGI
- enzyme binding Source: MGI
- growth factor binding Source: MGI
- identical protein binding Source: MGI
- interleukin-1 binding Source: MGI
- interleukin-8 binding Source: MGI
- nerve growth factor binding Source: MGI
- protease binding Source: MGI
- signaling receptor binding Source: MGI
- tumor necrosis factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 231245, 7 interactors |
IntActi | Q6GQT1, 1 interactor |
MINTi | Q6GQT1 |
STRINGi | 10090.ENSMUSP00000032203 |
Miscellaneous databases
RNActi | Q6GQT1, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 623 – 752 | Bait regionBy similarityAdd BLAST | 130 |
Sequence similaritiesi
Keywords - Domaini
Bait region, SignalPhylogenomic databases
eggNOGi | KOG1366, Eukaryota |
GeneTreei | ENSGT00940000154904 |
HOGENOMi | CLU_001634_0_1_1 |
InParanoidi | Q6GQT1 |
OMAi | CFGEESQ |
OrthoDBi | 354230at2759 |
PhylomeDBi | Q6GQT1 |
TreeFami | TF313285 |
Family and domain databases
CDDi | cd02897, A2M_2, 1 hit |
Gene3Di | 2.60.40.10, 2 hits 2.60.40.690, 1 hit |
InterProi | View protein in InterPro IPR009048, A-macroglobulin_rcpt-bd IPR036595, A-macroglobulin_rcpt-bd_sf IPR011625, A2M_N_BRD IPR041813, A2M_TED IPR011626, Alpha-macroglobulin_TED IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR001599, Macroglobln_a2 IPR019742, MacrogloblnA2_CS IPR002890, MG2 IPR041555, MG3 IPR040839, MG4 IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF00207, A2M, 1 hit PF07703, A2M_BRD, 1 hit PF07677, A2M_recep, 1 hit PF01835, MG2, 1 hit PF17791, MG3, 1 hit PF17789, MG4, 1 hit PF07678, TED_complement, 1 hit |
SMARTi | View protein in SMART SM01360, A2M, 1 hit SM01359, A2M_N_2, 1 hit SM01361, A2M_recep, 1 hit |
SUPFAMi | SSF48239, SSF48239, 1 hit SSF49410, SSF49410, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS00477, ALPHA_2_MACROGLOBULIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MGKRWLPSLA LLPLPPPLLL LLLLLLPTNA SAPQKPIYMV MVPSLLHAGT
60 70 80 90 100
PEKGCLLFNH LNETVTVKVS MESVRGNQSL FTDLVVDKDL FHCASFIVPQ
110 120 130 140 150
SSSNEVMFLT VQVKGPTHEF RRRSTVLIKT KESLVFAQTD KPIYKPGQMV
160 170 180 190 200
RFRVVSLDEN FHPLNELIPL LYIQDSKKNR IAQWQNFRLE GGLKQLSFPL
210 220 230 240 250
SSEPTQGSYK VVIRTESGRT VEHPFSVKEF VLPKFEVKVA VPETITILEE
260 270 280 290 300
EMNVSVCGIY TYGKPVPGHV TVNICRKYSN PSSCFGEESL AFCEKFSQQL
310 320 330 340 350
DGRGCFSQLV KTKSFQLKRQ EYEMQLDVNA KIQEEGTGVE ETGKGLTKIT
360 370 380 390 400
RTITKLSFVN VDTHFRQGIP FVGQVLLVDG RGTPIPYEMI FIGADEANQN
410 420 430 440 450
INTTTDKNGL ARFSINTDDI MGTSLTVRAK YKDSNVCYGF RWLTEENVEA
460 470 480 490 500
WHTANAVFSP SRSFVHLESL PYKLRCEQTL AVQAHYILND EAVLERKELV
510 520 530 540 550
FYYLMMAKGG IVRAGTHVLP VTQGHKKGHF SILISMETDL APVARLVLYT
560 570 580 590 600
ILPNGEVVGD TVKYEIEKCL ANKVDLVFHP NIGLPATRAF LSVMASPQSL
610 620 630 640 650
CGLRAVDQSV LLTKPEAELS ASLVYDLLPV KDLTGFPKGV NQQEEDTNGC
660 670 680 690 700
LKQNDTYING ILYSPVQNTN EEDMYGFLKD MGLKVFTNLN IRKPKVCERL
710 720 730 740 750
GVNKIPAAYH LVSQGHMDAF LESSESPTET TRSYFPETWI WDLVIVDSTG
760 770 780 790 800
VAEMEVTVPD TITEWKAGAF CLSNDTGLGL SPVIDFQAFQ PFFVDLTMPY
810 820 830 840 850
SVIRGEAFTL KATVLNYLQT CIRVGVQLEA SPDFLATPEE KEQKSHCICM
860 870 880 890 900
NERHTMSWAV IPKSLGNVNF TVSAEALDSK ELCRNEVPVV PERGKKDTII
910 920 930 940 950
KSLLVEPEGL ENEVTFNSLL CPTGAEVSEQ ISLKLPSDVV EESARASVTV
960 970 980 990 1000
LGDILGSAMQ NTQDLLKMPY GCGEQNMVLF APNIYVLDYL NETEQLTQEI
1010 1020 1030 1040 1050
KTKAITYLNT GYQRQLNYKH RDGSYSTFGD KPGRSHANTW LTAFVLKSFA
1060 1070 1080 1090 1100
QARRYIFIDE SHITQALTWL SQQQKDNGCF RSSGSLLNNA MKGGVEDEVT
1110 1120 1130 1140 1150
LSAYITIALL EMSLPVTHPV VRNALFCLDT AWKSARRGAS GNHVYTKALL
1160 1170 1180 1190 1200
AYAFALAGNQ DTKKEILKSL DEEAVKEDNS VHWTRAQKPR VPADLWYQPQ
1210 1220 1230 1240 1250
APSAEVEMTA YVLLAYLTTE LVPTREDLTA AMLIVKWLTK QQNSHGGFSS
1260 1270 1280 1290 1300
TQDTVVALHA LSKYGAATFT RAKKAAHVTI QSSGAFYTKF QVNNDNQLLL
1310 1320 1330 1340 1350
QRVTLPTVPG DYTAKVAGEG CVYLQTSLKY SVLPREKEFP FALVVQTLPG
1360 1370 1380 1390 1400
TCEDLKAHTT FQISLNISYI GSRSDSNMAI ADVKMVSGFI PLKPTVKMLE
1410 1420 1430 1440 1450
RSVHVSRTEV SNNHVLIYLD KVSNQMLTLF FMVQQDIPVR DLKPAIVKVY
1460 1470
DYYEKDEFAV AKYSAPCSAG YGNA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0N4SUX6 | A0A0N4SUX6_MOUSE | Alpha-2-macroglobulin-P | A2m | 35 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 366 | R → G in AAO25741 (PubMed:15355875).Curated | 1 | |
Sequence conflicti | 452 | H → R in AAO25741 (PubMed:15355875).Curated | 1 | |
Sequence conflicti | 659 – 666 | NGILYSPV → RNPVLPR in AAO25741 (PubMed:15355875).Curated | 8 | |
Sequence conflicti | 799 | P → A in AAH72642 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY185125 mRNA Translation: AAO25741.1 AC153581 Genomic DNA No translation available. BC072642 mRNA Translation: AAH72642.1 |
CCDSi | CCDS39617.1 |
RefSeqi | NP_783327.2, NM_175628.3 |
Genome annotation databases
Ensembli | ENSMUST00000032203; ENSMUSP00000032203; ENSMUSG00000030111 |
GeneIDi | 232345 |
KEGGi | mmu:232345 |
UCSCi | uc009doq.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY185125 mRNA Translation: AAO25741.1 AC153581 Genomic DNA No translation available. BC072642 mRNA Translation: AAH72642.1 |
CCDSi | CCDS39617.1 |
RefSeqi | NP_783327.2, NM_175628.3 |
3D structure databases
AlphaFoldDBi | Q6GQT1 |
SMRi | Q6GQT1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 231245, 7 interactors |
IntActi | Q6GQT1, 1 interactor |
MINTi | Q6GQT1 |
STRINGi | 10090.ENSMUSP00000032203 |
Protein family/group databases
MEROPSi | I39.004 |
PTM databases
GlyGeni | Q6GQT1, 9 sites |
iPTMneti | Q6GQT1 |
PhosphoSitePlusi | Q6GQT1 |
Proteomic databases
CPTACi | non-CPTAC-3311 non-CPTAC-3384 |
jPOSTi | Q6GQT1 |
MaxQBi | Q6GQT1 |
PaxDbi | Q6GQT1 |
PeptideAtlasi | Q6GQT1 |
PRIDEi | Q6GQT1 |
ProteomicsDBi | 286006 |
Protocols and materials databases
Antibodypediai | 859, 926 antibodies from 41 providers |
DNASUi | 232345 |
Genome annotation databases
Ensembli | ENSMUST00000032203; ENSMUSP00000032203; ENSMUSG00000030111 |
GeneIDi | 232345 |
KEGGi | mmu:232345 |
UCSCi | uc009doq.1, mouse |
Organism-specific databases
CTDi | 2 |
MGIi | MGI:2449119, A2m |
VEuPathDBi | HostDB:ENSMUSG00000030111 |
Phylogenomic databases
eggNOGi | KOG1366, Eukaryota |
GeneTreei | ENSGT00940000154904 |
HOGENOMi | CLU_001634_0_1_1 |
InParanoidi | Q6GQT1 |
OMAi | CFGEESQ |
OrthoDBi | 354230at2759 |
PhylomeDBi | Q6GQT1 |
TreeFami | TF313285 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-140837, Intrinsic Pathway of Fibrin Clot Formation R-MMU-1474228, Degradation of the extracellular matrix R-MMU-8963896, HDL assembly |
Miscellaneous databases
BioGRID-ORCSi | 232345, 3 hits in 73 CRISPR screens |
ChiTaRSi | A2m, mouse |
PROi | PR:Q6GQT1 |
RNActi | Q6GQT1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030111, Expressed in decidua and 209 other tissues |
ExpressionAtlasi | Q6GQT1, baseline and differential |
Genevisiblei | Q6GQT1, MM |
Family and domain databases
CDDi | cd02897, A2M_2, 1 hit |
Gene3Di | 2.60.40.10, 2 hits 2.60.40.690, 1 hit |
InterProi | View protein in InterPro IPR009048, A-macroglobulin_rcpt-bd IPR036595, A-macroglobulin_rcpt-bd_sf IPR011625, A2M_N_BRD IPR041813, A2M_TED IPR011626, Alpha-macroglobulin_TED IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR001599, Macroglobln_a2 IPR019742, MacrogloblnA2_CS IPR002890, MG2 IPR041555, MG3 IPR040839, MG4 IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF00207, A2M, 1 hit PF07703, A2M_BRD, 1 hit PF07677, A2M_recep, 1 hit PF01835, MG2, 1 hit PF17791, MG3, 1 hit PF17789, MG4, 1 hit PF07678, TED_complement, 1 hit |
SMARTi | View protein in SMART SM01360, A2M, 1 hit SM01359, A2M_N_2, 1 hit SM01361, A2M_recep, 1 hit |
SUPFAMi | SSF48239, SSF48239, 1 hit SSF49410, SSF49410, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS00477, ALPHA_2_MACROGLOBULIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | A2MG_MOUSE | |
Accessioni | Q6GQT1Primary (citable) accession number: Q6GQT1 Secondary accession number(s): E9QMQ7, Q811S0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 9, 2007 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 129 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families