UniProtKB - Q6GQQ9 (OTU7B_HUMAN)
OTU domain-containing protein 7B
OTUD7B
Functioni
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).6 Publications EC:3.4.19.12
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 191 | By similarity | 1 | |
Active sitei | 194 | Nucleophile4 Publications | 1 | |
Sitei | 197 | Stabilizes the conformation of the regulatory loop1 Publication | 1 | |
Active sitei | 358 | Proton acceptor1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 796 – 831 | A20-typePROSITE-ProRule annotationAdd BLAST | 36 |
GO - Molecular functioni
- cysteine-type peptidase activity Source: UniProtKB
- DNA binding Source: InterPro
- K63-linked polyubiquitin modification-dependent protein binding Source: GO_Central
- Lys48-specific deubiquitinase activity Source: ParkinsonsUK-UCL
- thiol-dependent ubiquitin-specific protease activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- in utero embryonic development Source: Ensembl
- mucosal immune response Source: UniProtKB
- negative regulation of I-kappaB kinase/NF-kappaB signaling Source: HGNC-UCL
- negative regulation of interleukin-8 production Source: ParkinsonsUK-UCL
- negative regulation of protein localization to nucleus Source: ParkinsonsUK-UCL
- negative regulation of transcription by RNA polymerase II Source: ParkinsonsUK-UCL
- protein deubiquitination Source: Reactome
- protein deubiquitination involved in ubiquitin-dependent protein catabolic process Source: GO_Central
- protein K11-linked deubiquitination Source: UniProtKB
- protein K48-linked deubiquitination Source: ParkinsonsUK-UCL
- protein K63-linked deubiquitination Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Adaptive immunity, Immunity, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q6GQQ9 |
Reactomei | R-HSA-5357786, TNFR1-induced proapoptotic signaling R-HSA-5357905, Regulation of TNFR1 signaling R-HSA-5357956, TNFR1-induced NFkappaB signaling pathway R-HSA-5689896, Ovarian tumor domain proteases |
SignaLinki | Q6GQQ9 |
Protein family/group databases
MEROPSi | C64.001 |
Names & Taxonomyi
Protein namesi | Recommended name: OTU domain-containing protein 7B (EC:3.4.19.126 Publications)Alternative name(s): Cellular zinc finger anti-NF-kappa-B protein1 Publication Short name: Cezanne3 Publications Zinc finger A20 domain-containing protein 1 Zinc finger protein CezanneCurated |
Gene namesi | Name:OTUD7B Synonyms:ZA20D1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16683, OTUD7B |
MIMi | 611748, gene |
neXtProti | NX_Q6GQQ9 |
VEuPathDBi | HostDB:ENSG00000264522.5 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 155 – 156 | LI → GG: Reduces deubiquitinating activity. 1 Publication | 2 | |
Mutagenesisi | 157 | E → K: Reduces deubiquitinating activity with 'Lys-11'-linked ubiquitin chains; no effect on cleavage of 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains. 1 Publication | 1 | |
Mutagenesisi | 193 | N → L or M: Loss of deubiquitinating activity due to stabilization of the autoinhibited conformation. 1 Publication | 1 | |
Mutagenesisi | 194 | C → A: Loss of deubiquitinating activity. Increased ability to interact with polyubiquitin. 4 Publications | 1 | |
Mutagenesisi | 194 | C → S: Loss of deubiquitinating activity. 3 Publications | 1 | |
Mutagenesisi | 194 | C → S: Loss of deubiquitinating activity; when associated with N-358. 1 Publication | 1 | |
Mutagenesisi | 197 | H → A or E: Strongly reduces deubiquitinating activity. 1 Publication | 1 | |
Mutagenesisi | 197 | H → D or N: Reduces deubiquitinating activity. 1 Publication | 1 | |
Mutagenesisi | 210 | D → A: Reduces deubiquitinating activity. 1 Publication | 1 | |
Mutagenesisi | 295 | E → K: Loss of deubiquitinating activity. 1 Publication | 1 | |
Mutagenesisi | 358 | H → A: Loss of deubiquitinating activity. 1 Publication | 1 | |
Mutagenesisi | 358 | H → N: Loss of deubiquitinating activity; when associated with S-194. 1 Publication | 1 | |
Mutagenesisi | 809 | F → A: Does not affect interaction with EGFR. 1 Publication | 1 | |
Mutagenesisi | 810 | Y → A: Impairs interaction with EGFR. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 56957 |
OpenTargetsi | ENSG00000264522 |
PharmGKBi | PA134873802 |
Miscellaneous databases
Pharosi | Q6GQQ9, Tbio |
Genetic variation databases
BioMutai | OTUD7B |
DMDMi | 51701318 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000188788 | 1 – 843 | OTU domain-containing protein 7BAdd BLAST | 843 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 100 | PhosphoserineCombined sources | 1 | |
Modified residuei | 464 | PhosphoserineBy similarity | 1 | |
Modified residuei | 467 | PhosphoserineCombined sources | 1 | |
Modified residuei | 471 | PhosphoserineBy similarity | 1 | |
Modified residuei | 729 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q6GQQ9 |
jPOSTi | Q6GQQ9 |
MassIVEi | Q6GQQ9 |
MaxQBi | Q6GQQ9 |
PaxDbi | Q6GQQ9 |
PeptideAtlasi | Q6GQQ9 |
PRIDEi | Q6GQQ9 |
ProteomicsDBi | 66318 [Q6GQQ9-1] |
PTM databases
iPTMneti | Q6GQQ9 |
PhosphoSitePlusi | Q6GQQ9 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000264522, Expressed in prostate gland and 216 other tissues |
ExpressionAtlasi | Q6GQQ9, baseline and differential |
Genevisiblei | Q6GQQ9, HS |
Organism-specific databases
HPAi | ENSG00000264522, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with ZAP70 in activated T cells, but not in resting T cells (PubMed:26903241).
Interacts with TRAF3 (By similarity).
Interacts with TRAF6 (PubMed:11463333).
Interacts with PARK7, leading to inhibit deubiquitinase activity (PubMed:21097510).
Interacts with EGFR, ITCH and NEDD4 (PubMed:22179831).
By similarity4 PublicationsBinary interactionsi
Q6GQQ9
Isoform 2 [Q6GQQ9-2]
GO - Molecular functioni
- K63-linked polyubiquitin modification-dependent protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 121281, 46 interactors |
DIPi | DIP-33805N |
IntActi | Q6GQQ9, 70 interactors |
STRINGi | 9606.ENSP00000462729 |
Miscellaneous databases
RNActi | Q6GQQ9, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q6GQQ9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 183 – 365 | OTUPROSITE-ProRule annotationAdd BLAST | 183 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 152 – 401 | TRAF-bindingAdd BLAST | 250 | |
Regioni | 167 – 440 | CatalyticAdd BLAST | 274 | |
Regioni | 187 – 193 | Regulatory loop1 Publication | 7 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 483 – 498 | Nuclear localization signalSequence analysisAdd BLAST | 16 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 796 – 831 | A20-typePROSITE-ProRule annotationAdd BLAST | 36 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4345, Eukaryota |
GeneTreei | ENSGT00940000159172 |
HOGENOMi | CLU_013263_0_0_1 |
InParanoidi | Q6GQQ9 |
OMAi | NVKWIPV |
OrthoDBi | 728724at2759 |
PhylomeDBi | Q6GQQ9 |
TreeFami | TF323312 |
Family and domain databases
InterProi | View protein in InterPro IPR003323, OTU_dom IPR002653, Znf_A20 |
Pfami | View protein in Pfam PF02338, OTU, 1 hit PF01754, zf-A20, 1 hit |
SMARTi | View protein in SMART SM00259, ZnF_A20, 1 hit |
PROSITEi | View protein in PROSITE PS50802, OTU, 1 hit PS51036, ZF_A20, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTLDMDAVLS DFVRSTGAEP GLARDLLEGK NWDVNAALSD FEQLRQVHAG
60 70 80 90 100
NLPPSFSEGS GGSRTPEKGF SDREPTRPPR PILQRQDDIV QEKRLSRGIS
110 120 130 140 150
HASSSIVSLA RSHVSSNGGG GGSNEHPLEM PICAFQLPDL TVYNEDFRSF
160 170 180 190 200
IERDLIEQSM LVALEQAGRL NWWVSVDPTS QRLLPLATTG DGNCLLHAAS
210 220 230 240 250
LGMWGFHDRD LMLRKALYAL MEKGVEKEAL KRRWRWQQTQ QNKESGLVYT
260 270 280 290 300
EDEWQKEWNE LIKLASSEPR MHLGTNGANC GGVESSEEPV YESLEEFHVF
310 320 330 340 350
VLAHVLRRPI VVVADTMLRD SGGEAFAPIP FGGIYLPLEV PASQCHRSPL
360 370 380 390 400
VLAYDQAHFS ALVSMEQKEN TKEQAVIPLT DSEYKLLPLH FAVDPGKGWE
410 420 430 440 450
WGKDDSDNVR LASVILSLEV KLHLLHSYMN VKWIPLSSDA QAPLAQPESP
460 470 480 490 500
TASAGDEPRS TPESGDSDKE SVGSSSTSNE GGRRKEKSKR DREKDKKRAD
510 520 530 540 550
SVANKLGSFG KTLGSKLKKN MGGLMHSKGS KPGGVGTGLG GSSGTETLEK
560 570 580 590 600
KKKNSLKSWK GGKEEAAGDG PVSEKPPAES VGNGGSKYSQ EVMQSLSILR
610 620 630 640 650
TAMQGEGKFI FVGTLKMGHR HQYQEEMIQR YLSDAEERFL AEQKQKEAER
660 670 680 690 700
KIMNGGIGGG PPPAKKPEPD AREEQPTGPP AESRAMAFST GYPGDFTIPR
710 720 730 740 750
PSGGGVHCQE PRRQLAGGPC VGGLPPYATF PRQCPPGRPY PHQDSIPSLE
760 770 780 790 800
PGSHSKDGLH RGALLPPPYR VADSYSNGYR EPPEPDGWAG GLRGLPPTQT
810 820 830 840
KCKQPNCSFY GHPETNNFCS CCYREELRRR EREPDGELLV HRF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5SZ59 | Q5SZ59_HUMAN | Ubiquitinyl hydrolase 1 | OTUD7B RP11-212K13.2-003 | 427 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 243 | K → E in AAH20622 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 421 | K → R in AAH20622 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 791 | G → S in BAH13129 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046015 | 805 – 833 | Missing in isoform 2. 1 PublicationAdd BLAST | 29 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ293573 mRNA Translation: CAB97494.1 Different initiation. AK299790 mRNA Translation: BAH13129.1 AL590487 Genomic DNA Translation: CAI12651.1 CH471121 Genomic DNA Translation: EAW53586.1 CH471121 Genomic DNA Translation: EAW53587.1 BC020622 mRNA Translation: AAH20622.1 BC072681 mRNA Translation: AAH72681.1 AL122102 mRNA Translation: CAB59268.1 |
CCDSi | CCDS72903.1 [Q6GQQ9-1] |
PIRi | T34535 |
RefSeqi | NP_064590.2, NM_020205.3 [Q6GQQ9-1] |
Genome annotation databases
Ensembli | ENST00000581312; ENSP00000462729; ENSG00000264522 [Q6GQQ9-1] |
GeneIDi | 56957 |
KEGGi | hsa:56957 |
UCSCi | uc001etn.5, human [Q6GQQ9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ293573 mRNA Translation: CAB97494.1 Different initiation. AK299790 mRNA Translation: BAH13129.1 AL590487 Genomic DNA Translation: CAI12651.1 CH471121 Genomic DNA Translation: EAW53586.1 CH471121 Genomic DNA Translation: EAW53587.1 BC020622 mRNA Translation: AAH20622.1 BC072681 mRNA Translation: AAH72681.1 AL122102 mRNA Translation: CAB59268.1 |
CCDSi | CCDS72903.1 [Q6GQQ9-1] |
PIRi | T34535 |
RefSeqi | NP_064590.2, NM_020205.3 [Q6GQQ9-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5LRU | X-ray | 2.20 | A | 129-438 | [»] | |
5LRV | X-ray | 2.80 | A | 129-438 | [»] | |
5LRW | X-ray | 2.00 | A/C | 129-266 | [»] | |
A/C | 292-438 | [»] | ||||
SMRi | Q6GQQ9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121281, 46 interactors |
DIPi | DIP-33805N |
IntActi | Q6GQQ9, 70 interactors |
STRINGi | 9606.ENSP00000462729 |
Protein family/group databases
MEROPSi | C64.001 |
PTM databases
iPTMneti | Q6GQQ9 |
PhosphoSitePlusi | Q6GQQ9 |
Genetic variation databases
BioMutai | OTUD7B |
DMDMi | 51701318 |
Proteomic databases
EPDi | Q6GQQ9 |
jPOSTi | Q6GQQ9 |
MassIVEi | Q6GQQ9 |
MaxQBi | Q6GQQ9 |
PaxDbi | Q6GQQ9 |
PeptideAtlasi | Q6GQQ9 |
PRIDEi | Q6GQQ9 |
ProteomicsDBi | 66318 [Q6GQQ9-1] |
Protocols and materials databases
Antibodypediai | 72463, 226 antibodies |
DNASUi | 56957 |
Genome annotation databases
Ensembli | ENST00000581312; ENSP00000462729; ENSG00000264522 [Q6GQQ9-1] |
GeneIDi | 56957 |
KEGGi | hsa:56957 |
UCSCi | uc001etn.5, human [Q6GQQ9-1] |
Organism-specific databases
CTDi | 56957 |
DisGeNETi | 56957 |
GeneCardsi | OTUD7B |
HGNCi | HGNC:16683, OTUD7B |
HPAi | ENSG00000264522, Low tissue specificity |
MIMi | 611748, gene |
neXtProti | NX_Q6GQQ9 |
OpenTargetsi | ENSG00000264522 |
PharmGKBi | PA134873802 |
VEuPathDBi | HostDB:ENSG00000264522.5 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4345, Eukaryota |
GeneTreei | ENSGT00940000159172 |
HOGENOMi | CLU_013263_0_0_1 |
InParanoidi | Q6GQQ9 |
OMAi | NVKWIPV |
OrthoDBi | 728724at2759 |
PhylomeDBi | Q6GQQ9 |
TreeFami | TF323312 |
Enzyme and pathway databases
PathwayCommonsi | Q6GQQ9 |
Reactomei | R-HSA-5357786, TNFR1-induced proapoptotic signaling R-HSA-5357905, Regulation of TNFR1 signaling R-HSA-5357956, TNFR1-induced NFkappaB signaling pathway R-HSA-5689896, Ovarian tumor domain proteases |
SignaLinki | Q6GQQ9 |
Miscellaneous databases
BioGRID-ORCSi | 56957, 9 hits in 994 CRISPR screens |
ChiTaRSi | OTUD7B, human |
GeneWikii | OTUD7B |
GenomeRNAii | 56957 |
Pharosi | Q6GQQ9, Tbio |
PROi | PR:Q6GQQ9 |
RNActi | Q6GQQ9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000264522, Expressed in prostate gland and 216 other tissues |
ExpressionAtlasi | Q6GQQ9, baseline and differential |
Genevisiblei | Q6GQQ9, HS |
Family and domain databases
InterProi | View protein in InterPro IPR003323, OTU_dom IPR002653, Znf_A20 |
Pfami | View protein in Pfam PF02338, OTU, 1 hit PF01754, zf-A20, 1 hit |
SMARTi | View protein in SMART SM00259, ZnF_A20, 1 hit |
PROSITEi | View protein in PROSITE PS50802, OTU, 1 hit PS51036, ZF_A20, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OTU7B_HUMAN | |
Accessioni | Q6GQQ9Primary (citable) accession number: Q6GQQ9 Secondary accession number(s): B7Z643 Q9UFF4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 31, 2004 |
Last sequence update: | July 19, 2004 | |
Last modified: | April 7, 2021 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families