UniProtKB - Q6GL57 (ILF3_XENTR)
Protein
Interleukin enhancer-binding factor 3
Gene
ilf3
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Functioni
RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs. As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (By similarity). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response. Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs. Has a cytoplasmic role early in development as part of a ribonucleoprotein (mRNP) complex which may regulate mRNA transport and/or translation. Following nuclear localization at the mid-blastula transition, acts as a transcription factor and binds the 5'-CCAAT-3' promoter sequence to regulate transcription of the gata2 gene as a subunit of the CCAAT box transcription factor (CBTF). Its role as an mRNP component negatively regulates its activity as a transcription factor by precluding its nuclear localization.By similarity
GO - Molecular functioni
- double-stranded RNA binding Source: UniProtKB
- mRNA 3'-UTR AU-rich region binding Source: GO_Central
- mRNA 5'-UTR binding Source: UniProtKB
- sequence-specific DNA binding Source: UniProtKB
- single-stranded RNA binding Source: GO_Central
- transcription regulatory region sequence-specific DNA binding Source: UniProtKB
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
- multicellular organism development Source: UniProtKB-KW
- regulation of transcription by RNA polymerase II Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, DNA-binding, RNA-binding |
Biological process | Antiviral defense, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Interleukin enhancer-binding factor 3Imported |
Gene namesi | Name:ilf3Imported |
Organismi | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
Taxonomic identifieri | 8364 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana |
Proteomesi |
|
Organism-specific databases
Xenbasei | XB-GENE-494367, ilf3 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Nucleus
- Nucleus By similarity
Note: Cytoplasmic in fertilized eggs, then translocates to the nucleus prior to gastrulation. RNA-binding is required for cytoplasmic retention during early development, and nuclear translocation at the mid-blastula transition (MBT) is probably coupled to the degradation of maternal mRNA that occurs at that stage (By similarity).By similarity
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- ribonucleoprotein complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000391691 | 1 – 698 | Interleukin enhancer-binding factor 3Add BLAST | 698 |
Post-translational modificationi
Phosphorylated. Phosphorylation affects nuclear translocation.By similarity
Methylated by protein arginine N-methyltransferase 1 (prmt1b) in the RGG-rich domain. Methylation decreases DNA-binding and thereby decreases transcription of the gata2 gene, but does not regulate dsRNA binding or subcellular localization.By similarity
Keywords - PTMi
Methylation, PhosphoproteinInteractioni
Subunit structurei
A component of a ybx2/frgy2-containing mRNA-ribonucleoprotein (mRNP) complex. Also a component of the CCAAT box transcription factor (CBTF) complex.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 379 | DZFPROSITE-ProRule annotationAdd BLAST | 375 | |
Domaini | 399 – 468 | DRBM 1PROSITE-ProRule annotationAdd BLAST | 70 | |
Domaini | 520 – 586 | DRBM 2PROSITE-ProRule annotationAdd BLAST | 67 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 372 – 390 | Bipartite nuclear localization signalSequence analysisAdd BLAST | 19 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 386 – 390 | Poly-LysSequence analysis | 5 | |
Compositional biasi | 605 – 670 | Arg/Gly-richSequence analysisAdd BLAST | 66 |
Keywords - Domaini
RepeatPhylogenomic databases
GeneTreei | ENSGT00940000156719 |
InParanoidi | Q6GL57 |
OMAi | YSHGQGN |
OrthoDBi | 612611at2759 |
Family and domain databases
Gene3Di | 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR014720, dsRBD_dom IPR006561, DZF_dom IPR033099, ILF3/NF90 IPR043519, NT_sf |
PANTHERi | PTHR45762:SF4, PTHR45762:SF4, 3 hits |
Pfami | View protein in Pfam PF00035, dsrm, 2 hits PF07528, DZF, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 2 hits SM00572, DZF, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 2 hits PS51703, DZF, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 14 potential isoforms that are computationally mapped.Show allAlign All
Q6GL57-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRPMRIFLND DRHVMAKHSV VYPTQEELEA VQNMVSHTER ALKAVSDWID
60 70 80 90 100
QQEKDCSGEQ EQPEPEEPET TEEGKDSEGK TGENPTRTLR GVMRVGLVAK
110 120 130 140 150
GLLLKGDLDL ELVLLCRDKP TISLLKRVAD NLVLQFETVS EDKYEVIQNI
160 170 180 190 200
REASIVIKNT KEPPLTLNIR LTSPLVREEM EKLSAGETLT VSDPPDVLDR
210 220 230 240 250
HKCLAALASL RHAKWFQARA NGLKSCVIVI RVLRDLCTRV PTWEPLRGWP
260 270 280 290 300
LELLCEKAIG TANRPMGAGE ALRRVLECLS SGILMPDGPG LYDPCEKEAS
310 320 330 340 350
DALEHLERQQ REDITQSAQH ALRLAAFGQL HKVLGMDPLP TKIPKKTKVE
360 370 380 390 400
TPTIDYTVQI PPSTTYAMPA LKRPMEEDGE DKSPSKKKKK IQKKDEKSEP
410 420 430 440 450
PQAMNALMRL NQLKPGLQYK LISQTGPVHA PIFTMSVEVD EKTFEASGPS
460 470 480 490 500
KKTAKLHVAV KVLQDMGLPT GMEEKEEATD ESEQKPVVQT PAQPADSTQT
510 520 530 540 550
DSAADQAESA KQQGPILTKH GKNPVMELNE KRRGLKYELI SETGGSHDKR
560 570 580 590 600
FVMEVEVDGV KFQGSGSNKK VAKAYAALAA LEKLFPDYTT YAEAPKKKRP
610 620 630 640 650
PMMPRGGPKF AGKHNQGFGM MYNEVPPPQA MRGRGRGGMN RGRGRGRGGF
660 670 680 690
GGGNHGGYMN SGGYGGGYGG NYNYQTSATA GYSDFFTDCY GYHDFASA
Computationally mapped potential isoform sequencesi
There are 14 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF7CF31 | F7CF31_XENTR | Aminomethyltransferase | amt ilf3 | 390 | Annotation score: | ||
F7EHJ3 | F7EHJ3_XENTR | Aminomethyltransferase | amt | 381 | Annotation score: | ||
A0A6I8QE17 | A0A6I8QE17_XENTR | Aminomethyltransferase | amt | 404 | Annotation score: | ||
A0A6I8QNW1 | A0A6I8QNW1_XENTR | Aminomethyltransferase | amt | 393 | Annotation score: | ||
A0A6I8R2J9 | A0A6I8R2J9_XENTR | Aminomethyltransferase | amt | 382 | Annotation score: | ||
A0A6I8R7M6 | A0A6I8R7M6_XENTR | Aminomethyltransferase | amt | 386 | Annotation score: | ||
A0A6I8SF62 | A0A6I8SF62_XENTR | Aminomethyltransferase | amt | 409 | Annotation score: | ||
A0A6I8SHW1 | A0A6I8SHW1_XENTR | Aminomethyltransferase | amt | 355 | Annotation score: | ||
F7ES74 | F7ES74_XENTR | Interleukin enhancer-binding factor... | ilf3 | 644 | Annotation score: | ||
A0A6I8RIC9 | A0A6I8RIC9_XENTR | Interleukin enhancer-binding factor... | ilf3 | 1,042 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC074653 mRNA Translation: AAH74653.1 |
RefSeqi | NP_001005648.1, NM_001005648.1 |
Genome annotation databases
Ensembli | ENSXETT00000049660; ENSXETP00000049660; ENSXETG00000011651 |
GeneIDi | 448121 |
KEGGi | xtr:448121 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC074653 mRNA Translation: AAH74653.1 |
RefSeqi | NP_001005648.1, NM_001005648.1 |
3D structure databases
SMRi | Q6GL57 |
ModBasei | Search... |
Genome annotation databases
Ensembli | ENSXETT00000049660; ENSXETP00000049660; ENSXETG00000011651 |
GeneIDi | 448121 |
KEGGi | xtr:448121 |
Organism-specific databases
CTDi | 3609 |
Xenbasei | XB-GENE-494367, ilf3 |
Phylogenomic databases
GeneTreei | ENSGT00940000156719 |
InParanoidi | Q6GL57 |
OMAi | YSHGQGN |
OrthoDBi | 612611at2759 |
Family and domain databases
Gene3Di | 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR014720, dsRBD_dom IPR006561, DZF_dom IPR033099, ILF3/NF90 IPR043519, NT_sf |
PANTHERi | PTHR45762:SF4, PTHR45762:SF4, 3 hits |
Pfami | View protein in Pfam PF00035, dsrm, 2 hits PF07528, DZF, 1 hit |
SMARTi | View protein in SMART SM00358, DSRM, 2 hits SM00572, DZF, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 2 hits PS51703, DZF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ILF3_XENTR | |
Accessioni | Q6GL57Primary (citable) accession number: Q6GL57 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 2, 2010 |
Last sequence update: | July 19, 2004 | |
Last modified: | April 7, 2021 | |
This is version 80 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |