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Protein

Endoplasmic reticulum chaperone BiP

Gene

KAR2

Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Activity regulationi

The chaperone activity is regulated by ATP-induced allosteric coupling of the nucleotide-binding (NBD) and substrate-binding (SBD) domains. In the ADP-bound and nucleotide-free (apo) states, the two domains have little interaction. In contrast, in the ATP-bound state the two domains are tightly coupled, which results in drastically accelerated kinetics in both binding and release of polypeptide substrates. J domain-containing co-chaperones stimulate the ATPase activity and are required for efficient substrate recognition.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 47ATPBy similarity4
Nucleotide bindingi233 – 235ATPBy similarity3
Nucleotide bindingi299 – 306ATPBy similarity8
Nucleotide bindingi370 – 373ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone, Hydrolase
Biological processStress response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum chaperone BiPCurated (EC:3.6.4.10By similarity)
Alternative name(s):
Immunoglobulin heavy chain-binding protein homologCurated
Short name:
BiPCurated
Gene namesi
Name:KAR2
Ordered Locus Names:CAGL0D02948g
OrganismiCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Taxonomic identifieri284593 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesNakaseomyces/Candida clade
Proteomesi
  • UP000002428 Componenti: Chromosome D

Organism-specific databases

CGDiCAL0128341 KAR2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000001358029 – 667Endoplasmic reticulum chaperone BiPAdd BLAST639

Proteomic databases

PRIDEiQ6FW50

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi284593.XP_445544.1

Structurei

3D structure databases

ProteinModelPortaliQ6FW50
SMRiQ6FW50
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni132 – 286Nucleotide-binding (NBD)By similarityAdd BLAST155
Regioni406 – 506Substrate-binding (SBD)By similarityAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi664 – 667Prevents secretion from ERBy similarity4

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0101 Eukaryota
COG0443 LUCA
HOGENOMiHOG000228135
InParanoidiQ6FW50
KOiK09490
OMAiCVGVMQK
OrthoDBiEOG092C1VPN

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6FW50-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNAIKRLLPV SLFLYALFVV LLPFQARAKD DVENYGTVIG IDLGTTYSCV
60 70 80 90 100
AVMKNGKTEI LANEQGNRIT PSYVAFTDDE RLIGDAAKNQ IAANPTNTIF
110 120 130 140 150
DIKRLIGLKF NDRSVQRDIK HLPFKVVDKD GKPVVQVKVK GEDRVFTPEE
160 170 180 190 200
ISGMILGKMK QIAEDYLGKK VTHAVVTVPA YFNDAQRQAT KDAGTIAGLN
210 220 230 240 250
VLRIVNEPTA AAIAYGLDKS DKEHQIIVYD LGGGTFDVSL LSIENGVFEV
260 270 280 290 300
QATSGDTHLG GEDFDYKIVR QLIKTFKKKH GIDVSDNEKA LAKLKREAEK
310 320 330 340 350
AKRALSSQMS TRIEIDSFVD GIDLSETLTR AKFEELNLDL FKKTLKPVEK
360 370 380 390 400
VLEDAGLEKK DIDDFVLVGG STRIPKVQQL LESFFNGKKA SKGINPDEAV
410 420 430 440 450
AYGAAVQAGV LSGEEGVEDI VLLDVNALTL GIETTGGVMT PLIKRNTAIP
460 470 480 490 500
TKKSQIFSTA VDNQPTVQIK VYEGERAMSK DNNLLGKFEL TGIPPAPRGI
510 520 530 540 550
PQIEVTFALD ANGILKVSAT DKGTGKSESI TITNDKGRLT QDEIDRMVEE
560 570 580 590 600
AEKFASEDAV LRTKIEARNK LENYAHSIKN QVEGDLAEKL EEEDKETLLD
610 620 630 640 650
AANDTLEWLE DNFDSATTEE FEEKFEALSK TAYPITSKLY GGADGSAPVY
660
DDDEDEDDDT DYFDDEL
Length:667
Mass (Da):73,330
Last modified:July 19, 2004 - v1
Checksum:iADC5674C6F87268B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380950 Genomic DNA Translation: CAG58455.1
RefSeqiXP_445544.1, XM_445544.1

Genome annotation databases

EnsemblFungiiCAG58455; CAG58455; CAGL0D02948g
GeneIDi2887030
KEGGicgr:CAGL0D02948g

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380950 Genomic DNA Translation: CAG58455.1
RefSeqiXP_445544.1, XM_445544.1

3D structure databases

ProteinModelPortaliQ6FW50
SMRiQ6FW50
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284593.XP_445544.1

Proteomic databases

PRIDEiQ6FW50

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG58455; CAG58455; CAGL0D02948g
GeneIDi2887030
KEGGicgr:CAGL0D02948g

Organism-specific databases

CGDiCAL0128341 KAR2

Phylogenomic databases

eggNOGiKOG0101 Eukaryota
COG0443 LUCA
HOGENOMiHOG000228135
InParanoidiQ6FW50
KOiK09490
OMAiCVGVMQK
OrthoDBiEOG092C1VPN

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBIP_CANGA
AccessioniPrimary (citable) accession number: Q6FW50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: July 19, 2004
Last modified: November 7, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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