UniProtKB - Q6FI81 (CPIN1_HUMAN)
Protein
Anamorsin
Gene
CIAPIN1
Organism
Homo sapiens (Human)
Status
Functioni
Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the scaffold complex NUBP1-NUBP2. Electrons are transferred to CIAPIN1 from NADPH via the FAD- and FMN-containing protein NDOR1 (PubMed:23596212). NDOR1-CIAPIN1 are also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit (By similarity). Has anti-apoptotic effects in the cell. Involved in negative control of cell death upon cytokine withdrawal. Promotes development of hematopoietic cells (By similarity).UniRule annotationBy similarity1 Publication
Miscellaneous
'Ana-mors-in' means 'anti-death molecule' in Latin.
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterUniRule annotation5 Publications
- [4Fe-4S] clusterUniRule annotation1 PublicationNote: In the presence of oxygen, the A site-bound [2Fe-2S] cluster is labile and the B site-bound [4Fe-4S] cluster is readily converted into a [2Fe-2S] cluster, a reason why recombinant protein is often isolated with a single [2Fe-2S] cluster.UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 237 | Iron-sulfur (2Fe-2S)UniRule annotation | 1 | |
Metal bindingi | 246 | Iron-sulfur (2Fe-2S)UniRule annotation | 1 | |
Metal bindingi | 249 | Iron-sulfur (2Fe-2S)UniRule annotation | 1 | |
Metal bindingi | 251 | Iron-sulfur (2Fe-2S)UniRule annotation | 1 | |
Metal bindingi | 274 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 277 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 285 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 288 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- electron transfer activity Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
- methyltransferase activity Source: InterPro
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- hemopoiesis Source: UniProtKB-UniRule
- iron-sulfur cluster assembly Source: GO_Central
- negative regulation of apoptotic process Source: UniProtKB
Keywordsi
Biological process | Apoptosis |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q6FI81 |
Reactomei | R-HSA-2564830, Cytosolic iron-sulfur cluster assembly |
Names & Taxonomyi
Protein namesi | Recommended name: AnamorsinUniRule annotationAlternative name(s): Cytokine-induced apoptosis inhibitor 1UniRule annotation Fe-S cluster assembly protein DRE2 homologUniRule annotation |
Gene namesi | Name:CIAPIN1UniRule annotation ORF Names:CUA001, PRO0915 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28050, CIAPIN1 |
MIMi | 608943, gene |
neXtProti | NX_Q6FI81 |
VEuPathDBi | HostDB:ENSG00000005194.14 |
Subcellular locationi
Mitochondrion
- Mitochondrion intermembrane space UniRule annotation1 Publication
Nucleus
- Nucleus UniRule annotation1 Publication
Other locations
- Cytoplasm UniRule annotation2 Publications
Mitochondrion
- mitochondrial intermembrane space Source: UniProtKB-SubCell
- mitochondrion Source: HPA
Nucleus
- nucleolus Source: UniProtKB
- nucleoplasm Source: HPA
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 57019 |
OpenTargetsi | ENSG00000005194 |
PharmGKBi | PA134978864 |
Miscellaneous databases
Pharosi | Q6FI81, Tbio |
Genetic variation databases
BioMutai | CIAPIN1 |
DMDMi | 57012667 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079288 | 1 – 312 | AnamorsinAdd BLAST | 312 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 136 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 177 | PhosphoserineCombined sources | 1 | |
Modified residuei | 182 | PhosphoserineCombined sources | 1 | |
Modified residuei | 183 | PhosphoserineCombined sources | 1 | |
Modified residuei | 215 | PhosphoserineCombined sources | 1 | |
Modified residuei | 272 | PhosphoserineBy similarity | 1 | |
Modified residuei | 305 | PhosphoserineCombined sources | 1 | |
Modified residuei | 307 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q6FI81 |
jPOSTi | Q6FI81 |
MassIVEi | Q6FI81 |
MaxQBi | Q6FI81 |
PaxDbi | Q6FI81 |
PeptideAtlasi | Q6FI81 |
PRIDEi | Q6FI81 |
ProteomicsDBi | 66295 [Q6FI81-1] 66296 [Q6FI81-2] 66297 [Q6FI81-3] |
TopDownProteomicsi | Q6FI81-1 [Q6FI81-1] |
PTM databases
iPTMneti | Q6FI81 |
PhosphoSitePlusi | Q6FI81 |
Expressioni
Tissue specificityi
Ubiquitously expressed. Highly expressed in heart, liver and pancreas.1 Publication
Gene expression databases
Bgeei | ENSG00000005194, Expressed in testis and 242 other tissues |
ExpressionAtlasi | Q6FI81, baseline and differential |
Genevisiblei | Q6FI81, HS |
Organism-specific databases
HPAi | ENSG00000005194, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
Interacts with NDOR1; its oxidized form can be reduced by NDOR1.
Interacts with CHCHD4 and may be a substrate for CHCHD4 chaperone activity.
2 PublicationsBinary interactionsi
Hide detailsQ6FI81
With | #Exp. | IntAct |
---|---|---|
CACNB3 [P54284] | 5 | EBI-750511,EBI-1184651 |
GLRX3 [O76003] | 18 | EBI-750511,EBI-374781 |
MEOX2 [P50222] | 3 | EBI-750511,EBI-748397 |
NDOR1 [Q9UHB4] | 13 | EBI-750511,EBI-10249760 |
Isoform 1 [Q6FI81-1]
With | #Exp. | IntAct |
---|---|---|
GLRX3 [O76003] | 2 | EBI-16172762,EBI-374781 |
Protein-protein interaction databases
BioGRIDi | 121328, 57 interactors |
DIPi | DIP-61697N |
IntActi | Q6FI81, 14 interactors |
MINTi | Q6FI81 |
STRINGi | 9606.ENSP00000377914 |
Miscellaneous databases
RNActi | Q6FI81, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q6FI81 |
SMRi | Q6FI81 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q6FI81 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 6 – 172 | N-terminal SAM-like domainUniRule annotationAdd BLAST | 167 | |
Regioni | 173 – 224 | LinkerUniRule annotationAdd BLAST | 52 | |
Regioni | 225 – 312 | Intrinsically disorderedUniRule annotationAdd BLAST | 88 | |
Regioni | 237 – 251 | Fe-S binding site AUniRule annotationAdd BLAST | 15 | |
Regioni | 274 – 288 | Fe-S binding site BUniRule annotationAdd BLAST | 15 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 274 – 277 | Cx2C motif 1UniRule annotation | 4 | |
Motifi | 285 – 288 | Cx2C motif 2UniRule annotation | 4 |
Domaini
The twin Cx2C motifs are involved in the recognition by the mitochondrial CHCHD4/MIA40-GFER/ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.UniRule annotation
The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.UniRule annotation
The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.UniRule annotation2 Publications
Sequence similaritiesi
Belongs to the anamorsin family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG4020, Eukaryota |
GeneTreei | ENSGT00390000011417 |
HOGENOMi | CLU_064393_2_0_1 |
InParanoidi | Q6FI81 |
OMAi | DWVLSCL |
OrthoDBi | 1588798at2759 |
PhylomeDBi | Q6FI81 |
TreeFami | TF314449 |
Family and domain databases
DisProti | DP01137 |
HAMAPi | MF_03115, Anamorsin, 1 hit |
InterProi | View protein in InterPro IPR007785, Anamorsin IPR013216, Methyltransf_11 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR13273, PTHR13273, 1 hit |
Pfami | View protein in Pfam PF05093, CIAPIN1, 2 hits PF08241, Methyltransf_11, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6FI81-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADFGISAGQ FVAVVWDKSS PVEALKGLVD KLQALTGNEG RVSVENIKQL
60 70 80 90 100
LQSAHKESSF DIILSGLVPG STTLHSAEIL AEIARILRPG GCLFLKEPVE
110 120 130 140 150
TAVDNNSKVK TASKLCSALT LSGLVEVKEL QREPLTPEEV QSVREHLGHE
160 170 180 190 200
SDNLLFVQIT GKKPNFEVGS SRQLKLSITK KSSPSVKPAV DPAAAKLWTL
210 220 230 240 250
SANDMEDDSM DLIDSDELLD PEDLKKPDPA SLRAASCGEG KKRKACKNCT
260 270 280 290 300
CGLAEELEKE KSREQMSSQP KSACGNCYLG DAFRCASCPY LGMPAFKPGE
310
KVLLSDSNLH DA
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BT65 | H3BT65_HUMAN | Anamorsin | CIAPIN1 | 266 | Annotation score: | ||
H3BV90 | H3BV90_HUMAN | Anamorsin | CIAPIN1 | 246 | Annotation score: | ||
H3BPG7 | H3BPG7_HUMAN | Anamorsin | CIAPIN1 | 239 | Annotation score: | ||
H3BQ23 | H3BQ23_HUMAN | Anamorsin | CIAPIN1 | 129 | Annotation score: | ||
H3BUG4 | H3BUG4_HUMAN | Anamorsin | CIAPIN1 | 188 | Annotation score: | ||
H3BTZ8 | H3BTZ8_HUMAN | Anamorsin | CIAPIN1 | 72 | Annotation score: | ||
H3BTT4 | H3BTT4_HUMAN | Anamorsin | CIAPIN1 | 48 | Annotation score: |
Sequence cautioni
The sequence AAC24312 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 142 | S → F in CAB66548 (PubMed:11230166).Curated | 1 | |
Sequence conflicti | 142 | S → F in CAG38576 (Ref. 4) Curated | 1 | |
Sequence conflicti | 278 | Y → C in CAG38576 (Ref. 4) Curated | 1 | |
Sequence conflicti | 289 | P → T in CAB66548 (PubMed:11230166).Curated | 1 | |
Sequence conflicti | 289 | P → T in CAG38576 (Ref. 4) Curated | 1 | |
Sequence conflicti | 310 | H → N in CAB66548 (PubMed:11230166).Curated | 1 | |
Sequence conflicti | 310 | H → N in CAG38576 (Ref. 4) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_033747 | 34 | A → E. Corresponds to variant dbSNP:rs11557672Ensembl. | 1 | |
Natural variantiVAR_033748 | 52 | Q → E. Corresponds to variant dbSNP:rs11557674Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012360 | 1 – 204 | Missing in isoform 2. 1 PublicationAdd BLAST | 204 | |
Alternative sequenceiVSP_012361 | 53 – 66 | SAHKE…IILSG → C in isoform 3. 3 PublicationsAdd BLAST | 14 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF248964 mRNA Translation: AAG44562.1 AL136613 mRNA Translation: CAB66548.1 AF116609 mRNA Translation: AAF71034.1 CR533545 mRNA Translation: CAG38576.1 AK292281 mRNA Translation: BAF84970.1 AC004382 Genomic DNA Translation: AAC24311.1 AC004382 Genomic DNA Translation: AAC24312.1 Sequence problems. CH471092 Genomic DNA Translation: EAW82923.1 BC002568 mRNA Translation: AAH02568.1 BC024196 mRNA Translation: AAH24196.1 BC067303 mRNA Translation: AAH67303.1 BC071740 mRNA Translation: AAH71740.1 |
CCDSi | CCDS10781.2 [Q6FI81-1] CCDS76876.1 [Q6FI81-3] |
RefSeqi | NP_001295276.1, NM_001308347.1 [Q6FI81-3] NP_001295287.1, NM_001308358.1 NP_064709.2, NM_020313.3 [Q6FI81-1] |
Genome annotation databases
Ensembli | ENST00000394391; ENSP00000377914; ENSG00000005194 [Q6FI81-1] ENST00000567518; ENSP00000456114; ENSG00000005194 [Q6FI81-3] |
GeneIDi | 57019 |
KEGGi | hsa:57019 |
UCSCi | uc002ell.2, human [Q6FI81-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF248964 mRNA Translation: AAG44562.1 AL136613 mRNA Translation: CAB66548.1 AF116609 mRNA Translation: AAF71034.1 CR533545 mRNA Translation: CAG38576.1 AK292281 mRNA Translation: BAF84970.1 AC004382 Genomic DNA Translation: AAC24311.1 AC004382 Genomic DNA Translation: AAC24312.1 Sequence problems. CH471092 Genomic DNA Translation: EAW82923.1 BC002568 mRNA Translation: AAH02568.1 BC024196 mRNA Translation: AAH24196.1 BC067303 mRNA Translation: AAH67303.1 BC071740 mRNA Translation: AAH71740.1 |
CCDSi | CCDS10781.2 [Q6FI81-1] CCDS76876.1 [Q6FI81-3] |
RefSeqi | NP_001295276.1, NM_001308347.1 [Q6FI81-3] NP_001295287.1, NM_001308358.1 NP_064709.2, NM_020313.3 [Q6FI81-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LD4 | NMR | - | A | 1-172 | [»] | |
2YUI | NMR | - | A | 1-170 | [»] | |
4M7R | X-ray | 1.80 | A/B | 1-172 | [»] | |
BMRBi | Q6FI81 | |||||
SMRi | Q6FI81 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121328, 57 interactors |
DIPi | DIP-61697N |
IntActi | Q6FI81, 14 interactors |
MINTi | Q6FI81 |
STRINGi | 9606.ENSP00000377914 |
PTM databases
iPTMneti | Q6FI81 |
PhosphoSitePlusi | Q6FI81 |
Genetic variation databases
BioMutai | CIAPIN1 |
DMDMi | 57012667 |
Proteomic databases
EPDi | Q6FI81 |
jPOSTi | Q6FI81 |
MassIVEi | Q6FI81 |
MaxQBi | Q6FI81 |
PaxDbi | Q6FI81 |
PeptideAtlasi | Q6FI81 |
PRIDEi | Q6FI81 |
ProteomicsDBi | 66295 [Q6FI81-1] 66296 [Q6FI81-2] 66297 [Q6FI81-3] |
TopDownProteomicsi | Q6FI81-1 [Q6FI81-1] |
Protocols and materials databases
Antibodypediai | 28857, 373 antibodies |
DNASUi | 57019 |
Genome annotation databases
Ensembli | ENST00000394391; ENSP00000377914; ENSG00000005194 [Q6FI81-1] ENST00000567518; ENSP00000456114; ENSG00000005194 [Q6FI81-3] |
GeneIDi | 57019 |
KEGGi | hsa:57019 |
UCSCi | uc002ell.2, human [Q6FI81-1] |
Organism-specific databases
CTDi | 57019 |
DisGeNETi | 57019 |
GeneCardsi | CIAPIN1 |
HGNCi | HGNC:28050, CIAPIN1 |
HPAi | ENSG00000005194, Low tissue specificity |
MIMi | 608943, gene |
neXtProti | NX_Q6FI81 |
OpenTargetsi | ENSG00000005194 |
PharmGKBi | PA134978864 |
VEuPathDBi | HostDB:ENSG00000005194.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4020, Eukaryota |
GeneTreei | ENSGT00390000011417 |
HOGENOMi | CLU_064393_2_0_1 |
InParanoidi | Q6FI81 |
OMAi | DWVLSCL |
OrthoDBi | 1588798at2759 |
PhylomeDBi | Q6FI81 |
TreeFami | TF314449 |
Enzyme and pathway databases
PathwayCommonsi | Q6FI81 |
Reactomei | R-HSA-2564830, Cytosolic iron-sulfur cluster assembly |
Miscellaneous databases
BioGRID-ORCSi | 57019, 575 hits in 888 CRISPR screens |
ChiTaRSi | CIAPIN1, human |
EvolutionaryTracei | Q6FI81 |
GeneWikii | CIAPIN1 |
GenomeRNAii | 57019 |
Pharosi | Q6FI81, Tbio |
PROi | PR:Q6FI81 |
RNActi | Q6FI81, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000005194, Expressed in testis and 242 other tissues |
ExpressionAtlasi | Q6FI81, baseline and differential |
Genevisiblei | Q6FI81, HS |
Family and domain databases
DisProti | DP01137 |
HAMAPi | MF_03115, Anamorsin, 1 hit |
InterProi | View protein in InterPro IPR007785, Anamorsin IPR013216, Methyltransf_11 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR13273, PTHR13273, 1 hit |
Pfami | View protein in Pfam PF05093, CIAPIN1, 2 hits PF08241, Methyltransf_11, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CPIN1_HUMAN | |
Accessioni | Q6FI81Primary (citable) accession number: Q6FI81 Secondary accession number(s): A8K8B6 Q9P1L7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 4, 2005 |
Last sequence update: | January 4, 2005 | |
Last modified: | February 10, 2021 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families