UniProtKB - Q6EV70 (OFUT1_RAT)
GDP-fucose protein O-fucosyltransferase 1
Pofut1
Functioni
Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs) (By similarity).
Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1.
By similarity2 PublicationsCatalytic activityi
- Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.1 Publication EC:2.4.1.221
Activity regulationi
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.2 PublicationsView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 347 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- fucosyltransferase activity Source: UniProtKB
- peptide-O-fucosyltransferase activity Source: RGD
GO - Biological processi
- angiogenesis Source: RGD
- fucose metabolic process Source: UniProtKB-KW
- heart development Source: RGD
- nervous system development Source: RGD
- Notch signaling pathway Source: RGD
- protein O-linked fucosylation Source: RGD
- protein O-linked glycosylation Source: UniProtKB
- regulation of Notch signaling pathway Source: UniProtKB
- somitogenesis Source: RGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Carbohydrate metabolism, Fucose metabolism, Notch signaling pathway |
Ligand | Manganese |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT65, Glycosyltransferase Family 65 |
Names & Taxonomyi
Protein namesi | Recommended name: GDP-fucose protein O-fucosyltransferase 1 (EC:2.4.1.2211 Publication)Alternative name(s): Peptide-O-fucosyltransferase 1 Short name: O-FucT-1 |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1303001, Pofut1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: RGD
Other locations
- membrane Source: UniProtKB
Keywords - Cellular componenti
Endoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 32 | Sequence analysisAdd BLAST | 32 | |
ChainiPRO_0000012151 | 33 – 395 | GDP-fucose protein O-fucosyltransferase 1Sequence analysisAdd BLAST | 363 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 45 ↔ 47 | By similarity | ||
Glycosylationi | 69 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 133 ↔ 147 | By similarity | ||
Glycosylationi | 167 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 256 ↔ 290 | By similarity | ||
Disulfide bondi | 274 ↔ 361 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | Q6EV70 |
PaxDbi | Q6EV70 |
PRIDEi | Q6EV70 |
PTM databases
GlyGeni | Q6EV70, 2 sites |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000010104, Expressed in liver and 21 other tissues |
Genevisiblei | Q6EV70, RN |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 50 – 53 | Substrate bindingBy similarity | 4 | |
Regioni | 245 – 247 | Substrate bindingBy similarity | 3 | |
Regioni | 364 – 365 | Substrate bindingBy similarity | 2 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 392 – 395 | Prevents secretion from ER | 4 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3849, Eukaryota |
GeneTreei | ENSGT00390000015634 |
HOGENOMi | CLU_039551_0_0_1 |
InParanoidi | Q6EV70 |
OMAi | RACANVE |
OrthoDBi | 1127619at2759 |
PhylomeDBi | Q6EV70 |
TreeFami | TF314805 |
Family and domain databases
CDDi | cd11302, O-FucT-1, 1 hit |
InterProi | View protein in InterPro IPR019378, GDP-Fuc_O-FucTrfase IPR039922, POFUT1 |
PANTHERi | PTHR21420, PTHR21420, 1 hit |
Pfami | View protein in Pfam PF10250, O-FucT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGAAAWAPSH LLLRASFLLL LLLLPLPLRG RAGGSWDLAG YLLYCPCMGR
60 70 80 90 100
FGNQADHFLG SLAFAKLLNR TLAVPPWIEY QHHKPPFTNL HVSYQKYFKL
110 120 130 140 150
EPLQAYHRVI SLEDFMEKLA PFHWPPEKRV AYCFEVAAQR SPDKKTCPMK
160 170 180 190 200
EGNPFGPFWD QFHVSFNKSE LFTGISFSAS YKEQWIQRFP PKEHPVLALP
210 220 230 240 250
GAPAQFPVLE EHRELQKYMV WSDEMVRTGE AQISTHLVRP YVGIHLRIGS
260 270 280 290 300
DWKNACAMLK DGTAGSHFMA SPQCVGYSRS TATPLTTTMC LPDLKEIQRA
310 320 330 340 350
VKLWVRALDA RSVYIATDSE SYVSEIQQLF KEKVKVVSLK PEVAQIDLYI
360 370 380 390
LGQADHFIGN CVSSFTAFVK RERDLHGRPS SFFGMDRPSQ LRDEF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ781499 mRNA Translation: CAH03711.1 |
RefSeqi | NP_001002278.1, NM_001002278.1 |
Genome annotation databases
Ensembli | ENSRNOT00000013730; ENSRNOP00000013730; ENSRNOG00000010104 |
GeneIDi | 311551 |
KEGGi | rno:311551 |
UCSCi | RGD:1303001, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ781499 mRNA Translation: CAH03711.1 |
RefSeqi | NP_001002278.1, NM_001002278.1 |
3D structure databases
SMRi | Q6EV70 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000013730 |
Protein family/group databases
CAZyi | GT65, Glycosyltransferase Family 65 |
PTM databases
GlyGeni | Q6EV70, 2 sites |
Proteomic databases
jPOSTi | Q6EV70 |
PaxDbi | Q6EV70 |
PRIDEi | Q6EV70 |
Genome annotation databases
Ensembli | ENSRNOT00000013730; ENSRNOP00000013730; ENSRNOG00000010104 |
GeneIDi | 311551 |
KEGGi | rno:311551 |
UCSCi | RGD:1303001, rat |
Organism-specific databases
CTDi | 23509 |
RGDi | 1303001, Pofut1 |
Phylogenomic databases
eggNOGi | KOG3849, Eukaryota |
GeneTreei | ENSGT00390000015634 |
HOGENOMi | CLU_039551_0_0_1 |
InParanoidi | Q6EV70 |
OMAi | RACANVE |
OrthoDBi | 1127619at2759 |
PhylomeDBi | Q6EV70 |
TreeFami | TF314805 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Miscellaneous databases
PROi | PR:Q6EV70 |
Gene expression databases
Bgeei | ENSRNOG00000010104, Expressed in liver and 21 other tissues |
Genevisiblei | Q6EV70, RN |
Family and domain databases
CDDi | cd11302, O-FucT-1, 1 hit |
InterProi | View protein in InterPro IPR019378, GDP-Fuc_O-FucTrfase IPR039922, POFUT1 |
PANTHERi | PTHR21420, PTHR21420, 1 hit |
Pfami | View protein in Pfam PF10250, O-FucT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | OFUT1_RAT | |
Accessioni | Q6EV70Primary (citable) accession number: Q6EV70 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | August 16, 2004 | |
Last modified: | September 29, 2021 | |
This is version 100 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families