UniProtKB - Q6ER94 (BAS1_ORYSJ)
Protein
2-Cys peroxiredoxin BAS1, chloroplastic
Gene
BAS1
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:16891402, PubMed:19345687). May be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf (By similarity).By similarity2 Publications
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity
Catalytic activityi
- EC:1.11.1.24By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 114 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- peroxiredoxin activity Source: UniProtKB
- thioredoxin peroxidase activity Source: GO_Central
GO - Biological processi
- cell redox homeostasis Source: GO_Central
- hydrogen peroxide catabolic process Source: UniProtKB
- response to oxidative stress Source: GO_Central
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Biological process | Hydrogen peroxide |
Protein family/group databases
PeroxiBasei | 4022, Os2CysPrx |
Names & Taxonomyi
Protein namesi | Recommended name: 2-Cys peroxiredoxin BAS1, chloroplastic (EC:1.11.1.24By similarity)Alternative name(s): Thiol-specific antioxidant protein Thioredoxin-dependent peroxiredoxin BAS1Curated |
Gene namesi | Name:BAS1 Ordered Locus Names:Os02g0537700, LOC_Os02g33450 ORF Names:OsJ_07037Imported, OSJNBa0014M17.2, P0508B05.23 |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
Subcellular locationi
Chloroplast
- chloroplast By similarity
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 114 | C → S: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 60 | Chloroplast1 PublicationAdd BLAST | 60 | |
ChainiPRO_0000284084 | 61 – 261 | 2-Cys peroxiredoxin BAS1, chloroplasticAdd BLAST | 201 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 114 | Interchain (with C-236); in linked formBy similarity | ||
Disulfide bondi | 236 | Interchain (with C-114); in linked formBy similarity |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | Q6ER94 |
PRIDEi | Q6ER94 |
Interactioni
Subunit structurei
Homodimer; disulfide-linked, upon oxidation (By similarity).
Interacts with the plastidial NADPH-dependent thioredoxin reductase NTRC (PubMed:19345687).
By similarity1 PublicationBinary interactionsi
Q6ER94
With | #Exp. | IntAct |
---|---|---|
Os07g0657900 [Q70G58] | 5 | EBI-6956385,EBI-6956411 |
Protein-protein interaction databases
IntActi | Q6ER94, 2 interactors |
MINTi | Q6ER94 |
STRINGi | 4530.OS02T0537700-01 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 68 – 227 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 160 |
Sequence similaritiesi
Keywords - Domaini
Redox-active center, Transit peptidePhylogenomic databases
eggNOGi | KOG0852, Eukaryota |
HOGENOMi | CLU_042529_21_0_1 |
InParanoidi | Q6ER94 |
OMAi | FWYPKDF |
OrthoDBi | 1326484at2759 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q6ER94-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAACCSSLAT AVSSSSAKPL AGIPPAAPHS LSLPRAPAAR PLRLSASSSR
60 70 80 90 100
SARASSFVAR AGGVDDAPLV GNKAPDFDAE AVFDQEFINV KLSDYIGKKY
110 120 130 140 150
VILFFYPLDF TFVCPTEITA FSDRYDEFEK LNTEILGVSI DSVFSHLAWV
160 170 180 190 200
QTDRKSGGLG DLKYPLISDV TKSISKSFGV LIPDQGIALR GLFIIDKEGV
210 220 230 240 250
IQHSTINNLA IGRSVDETMR TLQALQYVQD NPDEVCPAGW KPGDKSMKPD
260
PKGSKEYFAA I
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM039889 mRNA Translation: CAJ01693.1 AP004753 Genomic DNA Translation: BAD27915.1 AP005609 Genomic DNA Translation: BAD28826.1 AP008208 Genomic DNA Translation: BAF08964.1 AP014958 Genomic DNA Translation: BAS79077.1 CM000139 Genomic DNA Translation: EEE57135.1 AK068919 mRNA Translation: BAG91160.1 AK104703 mRNA Translation: BAG96892.1 |
RefSeqi | XP_015627405.1, XM_015771919.1 |
Genome annotation databases
EnsemblPlantsi | Os02t0537700-01; Os02t0537700-01; Os02g0537700 |
GeneIDi | 4329578 |
Gramenei | Os02t0537700-01; Os02t0537700-01; Os02g0537700 |
KEGGi | osa:4329578 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM039889 mRNA Translation: CAJ01693.1 AP004753 Genomic DNA Translation: BAD27915.1 AP005609 Genomic DNA Translation: BAD28826.1 AP008208 Genomic DNA Translation: BAF08964.1 AP014958 Genomic DNA Translation: BAS79077.1 CM000139 Genomic DNA Translation: EEE57135.1 AK068919 mRNA Translation: BAG91160.1 AK104703 mRNA Translation: BAG96892.1 |
RefSeqi | XP_015627405.1, XM_015771919.1 |
3D structure databases
SMRi | Q6ER94 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q6ER94, 2 interactors |
MINTi | Q6ER94 |
STRINGi | 4530.OS02T0537700-01 |
Protein family/group databases
PeroxiBasei | 4022, Os2CysPrx |
Proteomic databases
PaxDbi | Q6ER94 |
PRIDEi | Q6ER94 |
Genome annotation databases
EnsemblPlantsi | Os02t0537700-01; Os02t0537700-01; Os02g0537700 |
GeneIDi | 4329578 |
Gramenei | Os02t0537700-01; Os02t0537700-01; Os02g0537700 |
KEGGi | osa:4329578 |
Phylogenomic databases
eggNOGi | KOG0852, Eukaryota |
HOGENOMi | CLU_042529_21_0_1 |
InParanoidi | Q6ER94 |
OMAi | FWYPKDF |
OrthoDBi | 1326484at2759 |
Gene expression databases
Genevisiblei | Q6ER94, OS |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAS1_ORYSJ | |
Accessioni | Q6ER94Primary (citable) accession number: Q6ER94 Secondary accession number(s): B7EFL3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 17, 2007 |
Last sequence update: | August 16, 2004 | |
Last modified: | December 2, 2020 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations - SIMILARITY comments
Index of protein domains and families