UniProtKB - Q6EIG3 (CP26B_DANRE)
Protein
Cytochrome P450 26B1
Gene
cyp26b1
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation (PubMed:15661642). Appears to be involved in the inactivation of all-trans-retinoic acid (RA) (PubMed:15661642). Plays a role in skeletal development (PubMed:22019272).2 Publications
Catalytic activityi
- all-trans-retinoate + O2 + reduced [NADPH—hemoprotein reductase] = all-trans-4-hydroxyretinoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
hemeBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 440 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- monooxygenase activity Source: GO_Central
- oxidoreductase activity Source: GO_Central
- retinoic acid 4-hydroxylase activity Source: UniProtKB
GO - Biological processi
- bone morphogenesis Source: ZFIN
- cartilage morphogenesis Source: ZFIN
- coronal suture morphogenesis Source: ZFIN
- cranial suture morphogenesis Source: ZFIN
- embryonic cranial skeleton morphogenesis Source: ZFIN
- head morphogenesis Source: ZFIN
- hindbrain development Source: ZFIN
- negative regulation of retinoic acid receptor signaling pathway Source: UniProtKB
- odontogenesis Source: ZFIN
- oxidation-reduction process Source: GO_Central
- regulation of ossification Source: ZFIN
- retinoic acid catabolic process Source: ZFIN
- retinoic acid metabolic process Source: UniProtKB
- rhombomere 4 morphogenesis Source: ZFIN
- sterol metabolic process Source: GO_Central
- tendon formation Source: ZFIN
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-DRE-211916, Vitamins R-DRE-5365859, RA biosynthesis pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 26B1 (EC:1.14.13.-By similarity)Short name: Cyp26B1 Alternative name(s): Retinoic acid-metabolizing cytochrome |
Gene namesi | Name:cyp26b1Imported |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-030131-2908, cyp26b1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity
- Microsome membrane By similarity; Peripheral membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051986 | 1 – 511 | Cytochrome P450 26B1Add BLAST | 511 |
Proteomic databases
PaxDbi | Q6EIG3 |
Expressioni
Developmental stagei
Expressed zygotically. Weak expression at 75% epiboly. At the 2-somite stage, expressed in the presumptive rhombomere 4 (r4) region partially overlapping with the r3 region. At the 10-somite stage, expressed in the r4, r3 and r2 regions. At the 14-somite stage, expression extends to the r5 and r6 regions and is observed in the diencephalon. At 24-48 hours post-fertilization (hpf), additionally expressed in the eye, midbrain-hindbrain boundary, cerebellum, pectoral fin and the pharyngeal arch primordia.1 Publication
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Sequence analysis
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
InParanoidi | Q6EIG3 |
OrthoDBi | 871849at2759 |
PhylomeDBi | Q6EIG3 |
TreeFami | TF105093 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002403, Cyt_P450_E_grp-IV IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00465, EP450IV PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q6EIG3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLFESFDLVS ALATLAACLV SMALLLAVPQ QLWQLRWTAT RDKSCKLPMP
60 70 80 90 100
KGSMGFPIIG ETCHWFFQGA GFHASRRQKY GNVFKTHLLG RPLIRVTGAE
110 120 130 140 150
NVRKVLMGEH SLVTVDWPQS TSTLLGPNSL ANSIGDIHRK RRKIFAKVFS
160 170 180 190 200
HEALESYLPK IQQVIQETLR VWSSNPDPIN VYRESQRLSF NMAVRVLLGF
210 220 230 240 250
RIPEEEMHCL FSTFQEFVEN VFSLPIDLPF SGYRKGIRAR DSLQKSIEKA
260 270 280 290 300
IREKPLHTQG KDYTDALDVL LESAKENNTE LTMQELKEST IELIFAAFAT
310 320 330 340 350
TASASTSLVM QLLRHPAVLE KLREELRSCG LLHDGCLCQG ELRLDSIISL
360 370 380 390 400
KYLDCVIKEV LRLFAPVSGG YRIATQTFEL DGVQVPKGWS VMYSIRDTHD
410 420 430 440 450
TSAVFKDVEA FDPDRFSPER SEDREGRFHY LPFGGGVRSC LGKQLATLFL
460 470 480 490 500
KLLAVELAGG SRFELSTRTF PRMISVPVVH PTDGLRVKFF GLDSNQNQIM
510
AKSDEMLDAT V
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4I9F4 | A0A0R4I9F4_DANRE | Cytochrome P450 26B1 | cyp26b1 | 511 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 29 | P → S in AAH66759 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY321366 mRNA Translation: AAQ82596.1 BC066759 mRNA Translation: AAH66759.1 |
RefSeqi | NP_997831.1, NM_212666.1 |
Genome annotation databases
GeneIDi | 324188 |
KEGGi | dre:324188 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY321366 mRNA Translation: AAQ82596.1 BC066759 mRNA Translation: AAH66759.1 |
RefSeqi | NP_997831.1, NM_212666.1 |
3D structure databases
SMRi | Q6EIG3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000101543 |
Proteomic databases
PaxDbi | Q6EIG3 |
Genome annotation databases
GeneIDi | 324188 |
KEGGi | dre:324188 |
Organism-specific databases
CTDi | 56603 |
ZFINi | ZDB-GENE-030131-2908, cyp26b1 |
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
InParanoidi | Q6EIG3 |
OrthoDBi | 871849at2759 |
PhylomeDBi | Q6EIG3 |
TreeFami | TF105093 |
Enzyme and pathway databases
Reactomei | R-DRE-211916, Vitamins R-DRE-5365859, RA biosynthesis pathway |
Miscellaneous databases
PROi | PR:Q6EIG3 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002403, Cyt_P450_E_grp-IV IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00465, EP450IV PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CP26B_DANRE | |
Accessioni | Q6EIG3Primary (citable) accession number: Q6EIG3 Secondary accession number(s): Q6NY30 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | August 16, 2004 | |
Last modified: | December 2, 2020 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families