UniProtKB - Q6E1M8 (AWAT2_MOUSE)
Protein
Acyl-CoA wax alcohol acyltransferase 2
Gene
Awat2
Organism
Mus musculus (Mouse)
Status
Functioni
Acyltransferase that catalyzes the formation of ester bonds between fatty alcohols and fatty acyl-CoAs to form wax monoesters (PubMed:15220349). Shows a preference for medium chain acyl-CoAs from C12 to C16 in length and fatty alcohols shorter than C20, as the acyl donor and acceptor, respectively (PubMed:15220349). Also possesses acyl-CoA retinol acyltransferase (ARAT) activity that catalyzes 11-cis-specific retinyl ester synthesis (PubMed:28096191). Shows higher catalytic efficiency toward 11-cis-retinol versus 9-cis-retinol, 13- cis-retinol and all-trans-retinol substrates (By similarity).By similarity2 Publications
Catalytic activityi
- EC:2.3.1.752 PublicationsThis reaction proceeds in the forward2 Publications direction.
- EC:2.3.1.761 PublicationThis reaction proceeds in the forward1 Publication direction.
- 2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- (9Z)-hexadecen-1-ol + (9Z)-octadecenoyl-CoA = 1-O-(9Z)-hexadecenyl (9Z)-octadecenoate + CoABy similarity
- This reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecen-1-ol + (9Z)-octadecenoyl-CoA = 1-O-(9Z)-octadecenyl (9Z)-octadecenoate + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-glycerol = 1-O-(9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- (9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-glycerol = 1,2-di-(9Z-octadecenoyl)-glycerol + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Activity regulationi
11-cis retinoids act as allosteric modulators of acyl-CoA retinol O-fatty-acyltransferase (ARAT) activity by suppressing esterification of 9-cis, 13-cis, or all-trans retinols concurrently increasing the enzyme specificity toward 11-cis isomer.1 Publication
Kineticsi
- KM=26.22 µM for 11-cis-retinol1 Publication
- KM=22.25 µM for 9-cis-retinol1 Publication
- KM=24.32 µM for 9-cis-retinol (in the presence of 2 µM of 11-cis-retinyl palmitate)1 Publication
- KM=21.87 µM for 9-cis-retinol (in the presence of 4 µM of 11-cis-retinyl palmitate)1 Publication
- KM=18.74 µM for 9-cis-retinol (in the presence of 6 µM of 11-cis-retinyl palmitate)1 Publication
- KM=15.26 µM for 9-cis-retinol (in the presence of 10 µM of 11-cis-retinyl palmitate)1 Publication
- KM=35.18 µM for 13-cis-retinol1 Publication
- KM=23.59 µM for all-trans-retinol1 Publication
- Vmax=14.52 nmol/min/mg enzyme for 11-cis-retinol1 Publication
- Vmax=4.53 nmol/min/mg enzyme for 9-cis-retinol1 Publication
- Vmax=3.81 nmol/min/mg enzyme for 9-cis-retinol (in the presence of 2 µM of 11-cis-retinyl palmitate)1 Publication
- Vmax=2.61 nmol/min/mg enzyme for 9-cis-retinol (in the presence of 4 µM of 11-cis-retinyl palmitate)1 Publication
- Vmax=1.82 nmol/min/mg enzyme for 9-cis-retinol (in the presence of 6 µM of 11-cis-retinyl palmitate)1 Publication
- Vmax=0.96 nmol/min/mg enzyme for 9-cis-retinol (in the presence of 10 µM of 11-cis-retinyl palmitate)1 Publication
- Vmax=1.06 nmol/min/mg enzyme for 13-cis-retinol1 Publication
- Vmax=0.66 nmol/min/mg enzyme for all-trans-retinol1 Publication
GO - Molecular functioni
- arachidoyl-CoA:1-dodecanol O-acyltransferase activity Source: UniProtKB-EC
- long-chain-alcohol O-fatty-acyltransferase activity Source: MGI
- O-acyltransferase activity Source: GO_Central
- retinol O-fatty-acyltransferase activity Source: UniProtKB
GO - Biological processi
- lipid metabolic process Source: GO_Central
- monoacylglycerol biosynthetic process Source: UniProtKB
- wax biosynthetic process Source: MGI
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.75, 3474 |
Reactomei | R-MMU-1482883, Acyl chain remodeling of DAG and TAG R-MMU-2187335, The retinoid cycle in cones (daylight vision) R-MMU-9640463, Wax biosynthesis |
Chemistry databases
SwissLipidsi | SLP:000001829 |
Names & Taxonomyi
Protein namesi | Recommended name: Acyl-CoA wax alcohol acyltransferase 2 (EC:2.3.1.752 Publications)Alternative name(s): 11-cis-specific retinyl-ester synthase1 Publication Short name: 11-cis-RE-synthase1 Publication Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.761 Publication) Short name: ARAT Short name: Retinol O-fatty-acyltransferase Diacylglycerol O-acyltransferase 2-like protein 4 Long-chain-alcohol O-fatty-acyltransferase 2 Wax synthase Short name: mWS |
Gene namesi | Name:Awat2 Synonyms:Dgat2l4, Ws |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3045345, Awat2 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- endoplasmic reticulum membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 15 – 35 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 38 – 58 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 130 – 150 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249053 | 1 – 333 | Acyl-CoA wax alcohol acyltransferase 2Add BLAST | 333 |
Proteomic databases
PaxDbi | Q6E1M8 |
PRIDEi | Q6E1M8 |
PTM databases
PhosphoSitePlusi | Q6E1M8 |
Expressioni
Tissue specificityi
Expressed in Mueller cells of the retina (at protein level) (PubMed:24799687). Abundant in tissues rich in sebaceous glands such as the preputial gland and eyelid (PubMed:15220349).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000031220, Expressed in lip and 47 other tissues |
ExpressionAtlasi | Q6E1M8, baseline and differential |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000033567 |
Miscellaneous databases
RNActi | Q6E1M8, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the diacylglycerol acyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT00950000182761 |
HOGENOMi | CLU_023995_0_0_1 |
InParanoidi | Q6E1M8 |
OMAi | EVFAVFQ |
PhylomeDBi | Q6E1M8 |
TreeFami | TF314707 |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q6E1M8-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFWPTKKDLK TAMEVFALFQ WALSALVIVT TVIIVNLYLV VFTSYWPVTV
60 70 80 90 100
LMLTWLAFDW KTPERGGRRF TCVRKWRLWK HYSDYFPLKM VKTKDISPDR
110 120 130 140 150
NYILVCHPHG LMAHSCFGHF ATDTTGFSKT FPGITPYMLT LGAFFWVPFL
160 170 180 190 200
RDYVMSTGSC SVSRSSMDFL LTQKGTGNML VVVVGGLAEC RYSTPGSTTL
210 220 230 240 250
FLKKRQGFVR TALKHGVSLI PAYAFGETDL YDQHIFTPGG FVNRFQKWFQ
260 270 280 290 300
KMVHIYPCAF YGRGLTKNSW GLLPYSQPVT TVVGEPLPLP KIENPSEEIV
310 320 330
AKYHTLYIDA LRKLFDQHKT KFGISETQEL VIV
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020357 | 1 – 51 | Missing in isoform 2. 1 PublicationAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY611031 mRNA Translation: AAT68765.1 AY611032 mRNA Translation: AAT68766.1 AK034920 mRNA Translation: BAC28882.1 |
CCDSi | CCDS30300.1 [Q6E1M8-1] CCDS72412.1 [Q6E1M8-2] |
RefSeqi | NP_001277324.1, NM_001290395.1 [Q6E1M8-2] NP_808414.2, NM_177746.4 [Q6E1M8-1] XP_011245890.1, XM_011247588.2 [Q6E1M8-2] XP_011245892.1, XM_011247590.2 [Q6E1M8-2] XP_011245893.1, XM_011247591.2 [Q6E1M8-2] XP_011245894.1, XM_011247592.2 [Q6E1M8-2] XP_017173978.1, XM_017318489.1 [Q6E1M8-2] |
Genome annotation databases
Ensembli | ENSMUST00000033567; ENSMUSP00000033567; ENSMUSG00000031220 [Q6E1M8-1] ENSMUST00000147103; ENSMUSP00000128516; ENSMUSG00000031220 [Q6E1M8-2] |
GeneIDi | 245532 |
KEGGi | mmu:245532 |
UCSCi | uc009tvx.2, mouse [Q6E1M8-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY611031 mRNA Translation: AAT68765.1 AY611032 mRNA Translation: AAT68766.1 AK034920 mRNA Translation: BAC28882.1 |
CCDSi | CCDS30300.1 [Q6E1M8-1] CCDS72412.1 [Q6E1M8-2] |
RefSeqi | NP_001277324.1, NM_001290395.1 [Q6E1M8-2] NP_808414.2, NM_177746.4 [Q6E1M8-1] XP_011245890.1, XM_011247588.2 [Q6E1M8-2] XP_011245892.1, XM_011247590.2 [Q6E1M8-2] XP_011245893.1, XM_011247591.2 [Q6E1M8-2] XP_011245894.1, XM_011247592.2 [Q6E1M8-2] XP_017173978.1, XM_017318489.1 [Q6E1M8-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000033567 |
Chemistry databases
SwissLipidsi | SLP:000001829 |
PTM databases
PhosphoSitePlusi | Q6E1M8 |
Proteomic databases
PaxDbi | Q6E1M8 |
PRIDEi | Q6E1M8 |
Protocols and materials databases
Antibodypediai | 55918, 97 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000033567; ENSMUSP00000033567; ENSMUSG00000031220 [Q6E1M8-1] ENSMUST00000147103; ENSMUSP00000128516; ENSMUSG00000031220 [Q6E1M8-2] |
GeneIDi | 245532 |
KEGGi | mmu:245532 |
UCSCi | uc009tvx.2, mouse [Q6E1M8-1] |
Organism-specific databases
CTDi | 158835 |
MGIi | MGI:3045345, Awat2 |
Phylogenomic databases
eggNOGi | KOG0831, Eukaryota |
GeneTreei | ENSGT00950000182761 |
HOGENOMi | CLU_023995_0_0_1 |
InParanoidi | Q6E1M8 |
OMAi | EVFAVFQ |
PhylomeDBi | Q6E1M8 |
TreeFami | TF314707 |
Enzyme and pathway databases
BRENDAi | 2.3.1.75, 3474 |
Reactomei | R-MMU-1482883, Acyl chain remodeling of DAG and TAG R-MMU-2187335, The retinoid cycle in cones (daylight vision) R-MMU-9640463, Wax biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 245532, 1 hit in 17 CRISPR screens |
PROi | PR:Q6E1M8 |
RNActi | Q6E1M8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031220, Expressed in lip and 47 other tissues |
ExpressionAtlasi | Q6E1M8, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR007130, DAGAT |
Pfami | View protein in Pfam PF03982, DAGAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AWAT2_MOUSE | |
Accessioni | Q6E1M8Primary (citable) accession number: Q6E1M8 Secondary accession number(s): Q8BM49 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | August 16, 2004 | |
Last modified: | December 2, 2020 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families