UniProtKB - Q6DTM3 (AHI1_RAT)
Jouberin
Ahi1
Functioni
Involved in vesicle trafficking and required for ciliogenesis, formation of primary non-motile cilium, and recruitment of RAB8A to the basal body of primary cilium. Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in neuronal differentiation. As a positive modulator of classical Wnt signaling, may play a crucial role in ciliary signaling during cerebellum embryonic development.
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
GO - Biological processi
- cellular protein localization Source: UniProtKB
- central nervous system development Source: UniProtKB
- cilium assembly Source: UniProtKB
- cloaca development Source: UniProtKB
- embryonic brain development Source: RGD
- eye photoreceptor cell development Source: RGD
- heart looping Source: UniProtKB
- hindbrain development Source: UniProtKB
- Kupffer's vesicle development Source: UniProtKB
- left/right axis specification Source: UniProtKB
- morphogenesis of a polarized epithelium Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of glucose import Source: RGD
- non-motile cilium assembly Source: RGD
- otic vesicle development Source: UniProtKB
- photoreceptor cell outer segment organization Source: UniProtKB
- positive regulation of gene expression Source: RGD
- positive regulation of growth Source: RGD
- positive regulation of neuron projection development Source: RGD
- positive regulation of polarized epithelial cell differentiation Source: UniProtKB
- positive regulation of receptor internalization Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- pronephric duct morphogenesis Source: UniProtKB
- pronephric nephron tubule morphogenesis Source: UniProtKB
- protein localization to organelle Source: RGD
- regulation of behavior Source: UniProtKB
- regulation of protein secretion Source: RGD
- response to food Source: RGD
- retina development in camera-type eye Source: RGD
- retina layer formation Source: UniProtKB
- retinal cone cell development Source: RGD
- retinal rod cell development Source: RGD
- specification of axis polarity Source: UniProtKB
- spinal cord development Source: RGD
- transmembrane receptor protein tyrosine kinase signaling pathway Source: UniProtKB
- vesicle-mediated transport Source: UniProtKB
- vesicle targeting Source: RGD
Keywordsi
Molecular function | Developmental protein |
Biological process | Cilium biogenesis/degradation, Differentiation |
Enzyme and pathway databases
Reactomei | R-RNO-5620912, Anchoring of the basal body to the plasma membrane |
Names & Taxonomyi
Protein namesi | Recommended name: JouberinAlternative name(s): Abelson helper integration site 1 protein homolog Short name: AHI-1 |
Gene namesi | Name:Ahi1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1303040, Ahi1 |
Subcellular locationi
Cytoskeleton
- cilium basal body By similarity
- centriole By similarity
Other locations
- adherens junction By similarity
Note: In the retinal photoreceptor cell layer, localizes at the connecting cilium.By similarity
Cytoskeleton
- centriole Source: UniProtKB
- centrosome Source: UniProtKB
- ciliary basal body Source: UniProtKB
Other locations
- adherens junction Source: UniProtKB
- cell-cell junction Source: UniProtKB
- cilium Source: UniProtKB
- cytoplasm Source: RGD
- MKS complex Source: UniProtKB
- non-motile cilium Source: UniProtKB
- photoreceptor distal connecting cilium Source: RGD
- photoreceptor outer segment Source: RGD
Keywords - Cellular componenti
Cell junction, Cell projection, Cilium, Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050840 | 1 – 1047 | JouberinAdd BLAST | 1047 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 854 | PhosphoserineBy similarity | 1 | |
Modified residuei | 975 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q6DTM3 |
PRIDEi | Q6DTM3 |
PTM databases
iPTMneti | Q6DTM3 |
PhosphoSitePlusi | Q6DTM3 |
Interactioni
Subunit structurei
Self-associates. Part of the tectonic-like complex (also named B9 complex).
Interacts with MKS1.
Interacts with NPHP1; probably as heterodimers and/or AHI12:NPHP12 heterotetramers.
Interacts (via SH3 domain) with the dynamin GTPase DNM2.
Interacts with HAP1; probably as AHI12:HAP12 heterotetramers.
Interacts with RAB8A.
Interacts with CEND1 (By similarity).
Interacts with SPATA7 (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000019077 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 458 – 500 | WD 1Add BLAST | 43 | |
Repeati | 503 – 542 | WD 2Add BLAST | 40 | |
Repeati | 546 – 586 | WD 3Add BLAST | 41 | |
Repeati | 593 – 632 | WD 4Add BLAST | 40 | |
Repeati | 649 – 688 | WD 5Add BLAST | 40 | |
Repeati | 692 – 731 | WD 6Add BLAST | 40 | |
Repeati | 736 – 777 | WD 7Add BLAST | 42 | |
Domaini | 903 – 963 | SH3PROSITE-ProRule annotationAdd BLAST | 61 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 285 | Interaction with HAP1By similarityAdd BLAST | 285 | |
Regioni | 1 – 44 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 67 – 185 | DisorderedSequence analysisAdd BLAST | 119 | |
Regioni | 964 – 1047 | DisorderedSequence analysisAdd BLAST | 84 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 37 | Basic and acidic residuesSequence analysisAdd BLAST | 37 | |
Compositional biasi | 113 – 135 | Basic and acidic residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 158 – 185 | Basic and acidic residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 966 – 1041 | Basic and acidic residuesSequence analysisAdd BLAST | 76 |
Keywords - Domaini
Repeat, SH3 domain, WD repeatPhylogenomic databases
eggNOGi | KOG0266, Eukaryota |
InParanoidi | Q6DTM3 |
OrthoDBi | 1176970at2759 |
PhylomeDBi | Q6DTM3 |
Family and domain databases
CDDi | cd11812, SH3_AHI-1, 1 hit |
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR035832, AHI1_SH3 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00018, SH3_1, 1 hit PF00400, WD40, 2 hits |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00326, SH3, 1 hit SM00320, WD40, 7 hits |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MEQETPEKVD SAQEKVRGKS QPADDSEDSR EKAGTEGTGE LTEAYELQVA
60 70 80 90 100
EEMAKEIKKK IRRKLKEQLT YFPPDTLLHD DKLGSEKRKK KKKKKVPVPA
110 120 130 140 150
KPETSPSDVC DSAAEGEQKK EGAPEGSHHR EGGCSTEQNA DASVPENTKP
160 170 180 190 200
KPKKMKKKPK AVSEDNEETN GDGVHEITGR DSPVHPKCLL DDDLVMGVYI
210 220 230 240 250
HRTDRLKSDF MISHPMVKIH VVDEHTGQYV KKDDSERPVS SYYEKDNVDY
260 270 280 290 300
ILPIMTQPYD FKKLKSRLPE WEEQVIFNEN FPYLLREFDE CPKVILFFEI
310 320 330 340 350
LDFLSMDEIK NNSEFQNQEC GFRKIAWAFL KLLGANGNAN INSKLRLQLY
360 370 380 390 400
YPPTKPRSQP NVVEVFEWWS KCPRNRYPST LYVTVRGLKV PDCIKPSYRS
410 420 430 440 450
MMALQEERGT PVYCERHRET SSVDTEPGLE DSKEEVKWKR LPGQACRIPN
460 470 480 490 500
KHLFSLNAGE RGCFCLDFSH NGRILAAACA SRDGYPIILY EIPSGRFMRE
510 520 530 540 550
LCGHLNIIYD LDWSKDDRYL VTSSSDGTAR VWKNEINSTS TFRVLPHPSF
560 570 580 590 600
VYTARFHPAT RELVVTGCYD SMIRIWKVDA REDAAILVRQ LDVHKSFVNS
610 620 630 640 650
ICFDDEGHHM YSGDCIGVIA VWDTYVKVTD VQHSVRHWTI NKEIKETEFR
660 670 680 690 700
GVPVSYLEVH PNGKRLLIHT KDSTLRIMDL RILAARKFVG AANYREKIHS
710 720 730 740 750
TLTPCGTLLF SGSEDGIVYV WNPETGEQVA MYSELPFKST IRDISYHPFE
760 770 780 790 800
NMVAFCAFGQ SEPILLYIYD FQVAQQEAEM LKRYSGTVPL PGIHLSEDAL
810 820 830 840 850
CTCPKLPQQG SFQIDEFVNT ENNSSRKIQL VKQRLETVTE VIRSCAAKVN
860 870 880 890 900
KNLSITSPPP GPAKKPRVKQ SFVLTTDQII HQFGVPQTAF ISIERRPFMR
910 920 930 940 950
PVDPPPMVVA LYDYTASRSD ELTIHRGDII RVFFKDNEDW WYGSLGKGQE
960 970 980 990 1000
GFFPANHVAS ETLYRDSPPK VKERSPPLTP KEKAKMEKPP ASRKSLIKDR
1010 1020 1030 1040
FLDSRLGSKP MGHSEKGRDQ NFEERGHKSD MEMKKSEPTV RKVTLIE
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1M9F9 | F1M9F9_RAT | Jouberin | Ahi1 | 1,108 | Annotation score: | ||
A0A8I5ZQ93 | A0A8I5ZQ93_RAT | Jouberin | Ahi1 | 1,024 | Annotation score: | ||
A0A8I6A5B3 | A0A8I6A5B3_RAT | Jouberin | Ahi1 | 1,015 | Annotation score: | ||
A0A8I6AF27 | A0A8I6AF27_RAT | Jouberin | Ahi1 | 974 | Annotation score: | ||
A0A8I6AT56 | A0A8I6AT56_RAT | Jouberin | Ahi1 | 1,007 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY647140 mRNA Translation: AAT66919.1 |
RefSeqi | NP_001002277.1, NM_001002277.1 |
Genome annotation databases
GeneIDi | 308923 |
KEGGi | rno:308923 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY647140 mRNA Translation: AAT66919.1 |
RefSeqi | NP_001002277.1, NM_001002277.1 |
3D structure databases
AlphaFoldDBi | Q6DTM3 |
SMRi | Q6DTM3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000019077 |
PTM databases
iPTMneti | Q6DTM3 |
PhosphoSitePlusi | Q6DTM3 |
Proteomic databases
PaxDbi | Q6DTM3 |
PRIDEi | Q6DTM3 |
Genome annotation databases
GeneIDi | 308923 |
KEGGi | rno:308923 |
Organism-specific databases
CTDi | 54806 |
RGDi | 1303040, Ahi1 |
Phylogenomic databases
eggNOGi | KOG0266, Eukaryota |
InParanoidi | Q6DTM3 |
OrthoDBi | 1176970at2759 |
PhylomeDBi | Q6DTM3 |
Enzyme and pathway databases
Reactomei | R-RNO-5620912, Anchoring of the basal body to the plasma membrane |
Miscellaneous databases
PROi | PR:Q6DTM3 |
Family and domain databases
CDDi | cd11812, SH3_AHI-1, 1 hit |
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR035832, AHI1_SH3 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00018, SH3_1, 1 hit PF00400, WD40, 2 hits |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00326, SH3, 1 hit SM00320, WD40, 7 hits |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AHI1_RAT | |
Accessioni | Q6DTM3Primary (citable) accession number: Q6DTM3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2005 |
Last sequence update: | August 16, 2004 | |
Last modified: | May 25, 2022 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |