UniProtKB - Q6DJT9 (PLAG1_HUMAN)
Zinc finger protein PLAG1
PLAG1
Functioni
Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia.
3 PublicationsMiscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 34 – 56 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 62 – 86 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 92 – 114 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 121 – 143 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 150 – 172 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 185 – 207 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 213 – 236 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: ProtInc
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
GO - Biological processi
- gland morphogenesis Source: Ensembl
- multicellular organism growth Source: Ensembl
- negative regulation of gene expression Source: Ensembl
- positive regulation of gene expression Source: Ensembl
- positive regulation of glial cell proliferation Source: Ensembl
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- prostate gland growth Source: Ensembl
- regulation of transcription, DNA-templated Source: GO_Central
- transcription, DNA-templated Source: InterPro
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q6DJT9 |
SignaLinki | Q6DJT9 |
SIGNORi | Q6DJT9 |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein PLAG1Alternative name(s): Pleiomorphic adenoma gene 1 protein |
Gene namesi | Name:PLAG1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9045, PLAG1 |
MIMi | 603026, gene |
neXtProti | NX_Q6DJT9 |
VEuPathDBi | HostDB:ENSG00000181690 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Note: Strong nucleolar localization when sumoylation is inhibited.
Cytoskeleton
- centrosome Source: HPA
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear speck Source: HPA
- nucleoplasm Source: HPA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Involvement in diseasei
Silver-Russell syndrome 4 (SRS4)1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 23 – 24 | RK → AA: Inhibition of KPNA2 interaction when mutation occurs in the NLS; decreased nuclear import with localization in the nucleus but also in the cytoplasm; Complete inhibition of nuclear import when associated with a lack of zinc-finger domains. 1 Publication | 2 | |
Mutagenesisi | 31 – 32 | RK → AA: No inhibition of KPNA2 interaction and no change in nuclear import. 1 Publication | 2 | |
Mutagenesisi | 92 | H → A: Prevents formation of functional zinc-finger 3; induces drastic decrease of DNA affinity and complete modification of DNA binding specificity. 1 Publication | 1 | |
Mutagenesisi | 227 | H → A: Prevents formation of functional zinc-finger 7 and inhibits DNA binding; No proliferation and transformation of cultured cells. 2 Publications | 1 | |
Mutagenesisi | 244 | K → R: Abolishes single and double sumoylation; nuclear localization conserved. Increases transcriptional activity and inhibits repression domain activity; when associated with R-263 and R-353. 2 Publications | 1 | |
Mutagenesisi | 263 | K → R: Decreases sumoylation; Abolishes double sumoylation only; Nuclear localization conserved. Increases transcriptional activity and inhibits repression domain activity; when associated with R-244 and R-353. 2 Publications | 1 | |
Mutagenesisi | 339 | T → A: No effect on transcription activation capacity. 1 Publication | 1 | |
Mutagenesisi | 340 | S → A: No effect on transcription activation capacity. 1 Publication | 1 | |
Mutagenesisi | 353 | K → R: No effect on sumoylation. Increases transcriptional activity and inhibits repression domain activity; when associated with R-244 and R-263. 2 Publications | 1 |
Keywords - Diseasei
Dwarfism, Proto-oncogeneOrganism-specific databases
DisGeNETi | 5324 |
GeneReviewsi | PLAG1 |
MalaCardsi | PLAG1 |
MIMi | 181030, phenotype 618907, phenotype |
OpenTargetsi | ENSG00000181690 |
Orphaneti | 454821, Pleomorphic salivary gland adenoma 397590, Silver-Russell syndrome due to a point mutation |
PharmGKBi | PA33378 |
Miscellaneous databases
Pharosi | Q6DJT9, Tbio |
Genetic variation databases
BioMutai | PLAG1 |
DMDMi | 74757442 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000295107 | 1 – 500 | Zinc finger protein PLAG1Add BLAST | 500 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 244 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) | ||
Cross-linki | 263 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Ubl conjugationProteomic databases
EPDi | Q6DJT9 |
MassIVEi | Q6DJT9 |
MaxQBi | Q6DJT9 |
PaxDbi | Q6DJT9 |
PeptideAtlasi | Q6DJT9 |
PRIDEi | Q6DJT9 |
ProteomicsDBi | 4523 66229 [Q6DJT9-1] |
PTM databases
iPTMneti | Q6DJT9 |
PhosphoSitePlusi | Q6DJT9 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000181690, Expressed in intestine and 189 other tissues |
ExpressionAtlasi | Q6DJT9, baseline and differential |
Genevisiblei | Q6DJT9, HS |
Organism-specific databases
HPAi | ENSG00000181690, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with KPNA2, which escorts protein to the nucleus via interaction with nuclear localization signal.
Interacts with E3 SUMO-protein ligase PIAS1, PIAS2 and PIAS4.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 111340, 7 interactors |
IntActi | Q6DJT9, 5 interactors |
MINTi | Q6DJT9 |
STRINGi | 9606.ENSP00000325546 |
Miscellaneous databases
RNActi | Q6DJT9, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 30 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 2 – 84 | Interaction with KPNA21 PublicationAdd BLAST | 83 | |
Regioni | 41 – 242 | Decreased nuclear import with localization in the nucleus but also in the cytoplasmAdd BLAST | 202 | |
Regioni | 243 – 500 | Activates transcription; Inhibition of nuclear import due to lack of NLS and KPNA2 interactionAdd BLAST | 258 | |
Regioni | 243 – 384 | Repression domain; contains 3 sumoylation motifs and massively decrease transcription activityAdd BLAST | 142 | |
Regioni | 365 – 388 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 385 – 500 | Massively activates transcriptionAdd BLAST | 116 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 22 – 25 | Nuclear localization signal | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 365 – 385 | Polar residuesSequence analysisAdd BLAST | 21 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 34 – 56 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 62 – 86 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 92 – 114 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 121 – 143 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 150 – 172 | C2H2-type 5PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 185 – 207 | C2H2-type 6PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 213 – 236 | C2H2-type 7PROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000159246 |
HOGENOMi | CLU_002678_66_1_1 |
InParanoidi | Q6DJT9 |
OMAi | GTENRHD |
PhylomeDBi | Q6DJT9 |
TreeFami | TF332024 |
Family and domain databases
InterProi | View protein in InterPro IPR027765, PLAG1/PLAGL2 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR24390:SF64, PTHR24390:SF64, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 7 hits |
SUPFAMi | SSF57667, SSF57667, 4 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 7 hits PS50157, ZINC_FINGER_C2H2_2, 7 hits |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATVIPGDLS EVRDTQKVPS GKRKRGETKP RKNFPCQLCD KAFNSVEKLK
60 70 80 90 100
VHSYSHTGER PYKCIQQDCT KAFVSKYKLQ RHMATHSPEK THKCNYCEKM
110 120 130 140 150
FHRKDHLKNH LHTHDPNKET FKCEECGKNY NTKLGFKRHL ALHAATSGDL
160 170 180 190 200
TCKVCLQTFE STGVLLEHLK SHAGKSSGGV KEKKHQCEHC DRRFYTRKDV
210 220 230 240 250
RRHMVVHTGR KDFLCQYCAQ RFGRKDHLTR HMKKSHNQEL LKVKTEPVDF
260 270 280 290 300
LDPFTCNVSV PIKDELLPVM SLPSSELLSK PFTNTLQLNL YNTPFQSMQS
310 320 330 340 350
SGSAHQMITT LPLGMTCPID MDTVHPSHHL SFKYPFSSTS YAISIPEKEQ
360 370 380 390 400
PLKGEIESYL MELQGGVPSS SQDSQASSSS KLGLDPQIGS LDDGAGDLSL
410 420 430 440 450
SKSSISISDP LNTPALDFSQ LFNFIPLNGP PYNPLSVGSL GMSYSQEEAH
460 470 480 490 500
SSVSQLPPQT QDLQDPANTI GLGSLHSLSA AFTSSLSTST TLPRFHQAFQ
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 276 | E → D in BAD92368 (Ref. 3) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_033212 | 458 | P → T1 PublicationCorresponds to variant dbSNP:rs35883156Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045183 | 1 – 82 | Missing in isoform 2. 1 PublicationAdd BLAST | 82 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U65002 mRNA Translation: AAC50995.1 AK296933 mRNA Translation: BAG59484.1 AB209131 mRNA Translation: BAD92368.1 Different initiation. AC107952 Genomic DNA No translation available. BC075047 mRNA Translation: AAH75047.1 BC075048 mRNA Translation: AAH75048.1 |
CCDSi | CCDS47860.1 [Q6DJT9-2] CCDS6165.1 [Q6DJT9-1] |
RefSeqi | NP_001108106.1, NM_001114634.1 [Q6DJT9-1] NP_001108107.1, NM_001114635.1 [Q6DJT9-2] NP_002646.2, NM_002655.2 [Q6DJT9-1] XP_011515846.1, XM_011517544.2 [Q6DJT9-2] XP_016869065.1, XM_017013576.1 [Q6DJT9-1] XP_016869066.1, XM_017013577.1 [Q6DJT9-2] |
Genome annotation databases
Ensembli | ENST00000316981.8; ENSP00000325546.3; ENSG00000181690.8 ENST00000423799.6; ENSP00000404067.2; ENSG00000181690.8 [Q6DJT9-2] ENST00000429357.2; ENSP00000416537.2; ENSG00000181690.8 |
GeneIDi | 5324 |
KEGGi | hsa:5324 |
MANE-Selecti | ENST00000316981.8; ENSP00000325546.3; NM_002655.3; NP_002646.2 |
UCSCi | uc003xsr.5, human [Q6DJT9-1] |
Keywords - Coding sequence diversityi
Alternative splicing, Chromosomal rearrangementSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U65002 mRNA Translation: AAC50995.1 AK296933 mRNA Translation: BAG59484.1 AB209131 mRNA Translation: BAD92368.1 Different initiation. AC107952 Genomic DNA No translation available. BC075047 mRNA Translation: AAH75047.1 BC075048 mRNA Translation: AAH75048.1 |
CCDSi | CCDS47860.1 [Q6DJT9-2] CCDS6165.1 [Q6DJT9-1] |
RefSeqi | NP_001108106.1, NM_001114634.1 [Q6DJT9-1] NP_001108107.1, NM_001114635.1 [Q6DJT9-2] NP_002646.2, NM_002655.2 [Q6DJT9-1] XP_011515846.1, XM_011517544.2 [Q6DJT9-2] XP_016869065.1, XM_017013576.1 [Q6DJT9-1] XP_016869066.1, XM_017013577.1 [Q6DJT9-2] |
3D structure databases
AlphaFoldDBi | Q6DJT9 |
SMRi | Q6DJT9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111340, 7 interactors |
IntActi | Q6DJT9, 5 interactors |
MINTi | Q6DJT9 |
STRINGi | 9606.ENSP00000325546 |
PTM databases
iPTMneti | Q6DJT9 |
PhosphoSitePlusi | Q6DJT9 |
Genetic variation databases
BioMutai | PLAG1 |
DMDMi | 74757442 |
Proteomic databases
EPDi | Q6DJT9 |
MassIVEi | Q6DJT9 |
MaxQBi | Q6DJT9 |
PaxDbi | Q6DJT9 |
PeptideAtlasi | Q6DJT9 |
PRIDEi | Q6DJT9 |
ProteomicsDBi | 4523 66229 [Q6DJT9-1] |
Protocols and materials databases
Antibodypediai | 1454, 258 antibodies from 26 providers |
DNASUi | 5324 |
Genome annotation databases
Ensembli | ENST00000316981.8; ENSP00000325546.3; ENSG00000181690.8 ENST00000423799.6; ENSP00000404067.2; ENSG00000181690.8 [Q6DJT9-2] ENST00000429357.2; ENSP00000416537.2; ENSG00000181690.8 |
GeneIDi | 5324 |
KEGGi | hsa:5324 |
MANE-Selecti | ENST00000316981.8; ENSP00000325546.3; NM_002655.3; NP_002646.2 |
UCSCi | uc003xsr.5, human [Q6DJT9-1] |
Organism-specific databases
CTDi | 5324 |
DisGeNETi | 5324 |
GeneCardsi | PLAG1 |
GeneReviewsi | PLAG1 |
HGNCi | HGNC:9045, PLAG1 |
HPAi | ENSG00000181690, Low tissue specificity |
MalaCardsi | PLAG1 |
MIMi | 181030, phenotype 603026, gene 618907, phenotype |
neXtProti | NX_Q6DJT9 |
OpenTargetsi | ENSG00000181690 |
Orphaneti | 454821, Pleomorphic salivary gland adenoma 397590, Silver-Russell syndrome due to a point mutation |
PharmGKBi | PA33378 |
VEuPathDBi | HostDB:ENSG00000181690 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000159246 |
HOGENOMi | CLU_002678_66_1_1 |
InParanoidi | Q6DJT9 |
OMAi | GTENRHD |
PhylomeDBi | Q6DJT9 |
TreeFami | TF332024 |
Enzyme and pathway databases
PathwayCommonsi | Q6DJT9 |
SignaLinki | Q6DJT9 |
SIGNORi | Q6DJT9 |
Miscellaneous databases
BioGRID-ORCSi | 5324, 9 hits in 1097 CRISPR screens |
ChiTaRSi | PLAG1, human |
GeneWikii | PLAG1 |
GenomeRNAii | 5324 |
Pharosi | Q6DJT9, Tbio |
PROi | PR:Q6DJT9 |
RNActi | Q6DJT9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000181690, Expressed in intestine and 189 other tissues |
ExpressionAtlasi | Q6DJT9, baseline and differential |
Genevisiblei | Q6DJT9, HS |
Family and domain databases
InterProi | View protein in InterPro IPR027765, PLAG1/PLAGL2 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR24390:SF64, PTHR24390:SF64, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 7 hits |
SUPFAMi | SSF57667, SSF57667, 4 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 7 hits PS50157, ZINC_FINGER_C2H2_2, 7 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PLAG1_HUMAN | |
Accessioni | Q6DJT9Primary (citable) accession number: Q6DJT9 Secondary accession number(s): B4DLC2, Q59GH8, Q9Y4L2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 10, 2007 |
Last sequence update: | August 16, 2004 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families