UniProtKB - Q6DHV7 (ADAL_HUMAN)
Protein
Adenosine deaminase-like protein
Gene
ADAL
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the hydrolysis of the free cytosolic methylated adenosine nucleotide N6-methyl-AMP (N6-mAMP) to produce inositol monophosphate (IMP) and methylamine (PubMed:21755941, PubMed:29884623). Is required for the catabolism of cytosolic N6-mAMP, which is derived from the degradation of mRNA containing N6-methylated adenine (m6A) (PubMed:21755941, PubMed:29884623). Catalyzes the removal of different alkyl groups not only from N6-substituted purine or 2-aminopurine nucleoside monophosphates but also from O6-substituted compounds in vitro (PubMed:21755941).2 Publications
Catalytic activityi
- This reaction proceeds in the forward2 Publications direction.
Cofactori
Zn2+2 PublicationsNote: Binds 1 zinc ion per subunit.1 Publication
Kineticsi
- KM=12.5 µM for N6-methyl-AMP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 24 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 26 | Zinc; catalyticBy similarity | 1 | |
Binding sitei | 26 | N(6)-methyl-AMPBy similarity | 1 | |
Binding sitei | 28 | N(6)-methyl-AMPBy similarity | 1 | |
Binding sitei | 74 | N(6)-methyl-AMP; via tele nitrogenBy similarity | 1 | |
Binding sitei | 148 | N(6)-methyl-AMPBy similarity | 1 | |
Binding sitei | 181 | N(6)-methyl-AMP; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 208 | Zinc; catalyticBy similarity | 1 | |
Active sitei | 211 | Proton donorBy similarity | 1 | |
Binding sitei | 211 | N(6)-methyl-AMPBy similarity | 1 | |
Sitei | 232 | Important for catalytic activityBy similarity | 1 | |
Metal bindingi | 293 | Zinc; catalyticBy similarity | 1 | |
Binding sitei | 293 | N(6)-methyl-AMPBy similarity | 1 | |
Binding sitei | 294 | N(6)-methyl-AMPBy similarity | 1 |
GO - Molecular functioni
- adenosine deaminase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- N6-mAMP deaminase activity Source: RHEA
GO - Biological processi
- adenosine catabolic process Source: GO_Central
- drug metabolic process Source: Reactome
- inosine biosynthetic process Source: GO_Central
- nucleotide metabolic process Source: UniProtKB-KW
- purine-containing compound salvage Source: Reactome
Keywordsi
Molecular function | Hydrolase |
Biological process | Nucleotide metabolism |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000168803-MONOMER |
PathwayCommonsi | Q6DHV7 |
Reactomei | R-HSA-2161541, Abacavir metabolism R-HSA-74217, Purine salvage |
Names & Taxonomyi
Protein namesi | Recommended name: Adenosine deaminase-like protein (EC:3.5.4.-2 Publications)Alternative name(s): Adenosine deaminase-like protein isoform 11 Publication N6-mAMP deaminase1 Publication Short name: HsMAPDA1 Publication N6-methyl-AMP aminohydrolase1 Publication |
Gene namesi | Name:ADAL Synonyms:ADAL11 Publication |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:31853, ADAL |
neXtProti | NX_Q6DHV7 |
VEuPathDBi | HostDB:ENSG00000168803.14 |
Pathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000168803 |
PharmGKBi | PA142672643 |
Miscellaneous databases
Pharosi | Q6DHV7, Tbio |
Chemistry databases
ChEMBLi | CHEMBL1795150 |
Genetic variation databases
BioMutai | ADAL |
DMDMi | 146286026 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000285090 | 1 – 355 | Adenosine deaminase-like proteinAdd BLAST | 355 |
Proteomic databases
EPDi | Q6DHV7 |
jPOSTi | Q6DHV7 |
MassIVEi | Q6DHV7 |
MaxQBi | Q6DHV7 |
PeptideAtlasi | Q6DHV7 |
PRIDEi | Q6DHV7 |
ProteomicsDBi | 66225 [Q6DHV7-1] 66226 [Q6DHV7-2] 66227 [Q6DHV7-3] |
PTM databases
iPTMneti | Q6DHV7 |
Expressioni
Gene expression databases
Bgeei | ENSG00000168803, Expressed in cardiac muscle of right atrium and 180 other tissues |
ExpressionAtlasi | Q6DHV7, baseline and differential |
Genevisiblei | Q6DHV7, HS |
Organism-specific databases
HPAi | ENSG00000168803, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
1 PublicationBinary interactionsi
Hide detailsIsoform 2 [Q6DHV7-2]
Protein-protein interaction databases
BioGRIDi | 127803, 4 interactors |
IntActi | Q6DHV7, 17 interactors |
STRINGi | 9606.ENSP00000398744 |
Miscellaneous databases
RNActi | Q6DHV7, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 106 – 109 | N(6)-methyl-AMP bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.Curated
Phylogenomic databases
eggNOGi | KOG1097, Eukaryota |
GeneTreei | ENSGT00950000183113 |
HOGENOMi | CLU_039228_6_0_1 |
InParanoidi | Q6DHV7 |
OMAi | RPQFKPY |
PhylomeDBi | Q6DHV7 |
TreeFami | TF314270 |
Family and domain databases
InterProi | View protein in InterPro IPR001365, A/AMP_deaminase_dom IPR032466, Metal_Hydrolase |
Pfami | View protein in Pfam PF00962, A_deaminase, 1 hit |
SUPFAMi | SSF51556, SSF51556, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6DHV7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIEAEEQQPC KTDFYSELPK VELHAHLNGS ISSHTMKKLI AQKPDLKIHD
60 70 80 90 100
QMTVIDKGKK RTLEECFQMF QTIHQLTSSP EDILMVTKDV IKEFADDGVK
110 120 130 140 150
YLELRSTPRR ENATGMTKKT YVESILEGIK QSKQENLDID VRYLIAVDRR
160 170 180 190 200
GGPLVAKETV KLAEEFFLST EGTVLGLDLS GDPTVGQAKD FLEPLLEAKK
210 220 230 240 250
AGLKLALHLS EIPNQKKETQ ILLDLLPDRI GHGTFLNSGE GGSLDLVDFV
260 270 280 290 300
RQHRIPLELC LTSNVKSQTV PSYDQHHFGF WYSIAHPSVI CTDDKGVFAT
310 320 330 340 350
HLSQEYQLAA ETFNLTQSQV WDLSYESINY IFASDSTRSE LRKKWNHLKP
RVLHI
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BUD6 | H3BUD6_HUMAN | Adenosine deaminase-like protein | ADAL | 110 | Annotation score: | ||
H3BQR9 | H3BQR9_HUMAN | Adenosine deaminase-like protein | ADAL | 35 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 119 | K → E in AK126583 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 139 | I → T in AK126583 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042782 | 185 – 211 | Missing in isoform 3. 1 PublicationAdd BLAST | 27 | |
Alternative sequenceiVSP_024821 | 258 – 355 | ELCLT…RVLHI → GKAWSFRSSR in isoform 2. 1 PublicationAdd BLAST | 98 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK126583 mRNA No translation available. AK298870 mRNA Translation: BAG60990.1 AC009852 Genomic DNA No translation available. AC018924 Genomic DNA No translation available. BC075857 mRNA Translation: AAH75857.1 |
CCDSi | CCDS32214.1 [Q6DHV7-2] CCDS53936.1 [Q6DHV7-3] CCDS81868.1 [Q6DHV7-1] |
RefSeqi | NP_001012987.1, NM_001012969.3 [Q6DHV7-2] NP_001152752.1, NM_001159280.2 [Q6DHV7-3] NP_001311293.1, NM_001324364.1 [Q6DHV7-2] NP_001311294.1, NM_001324365.1 [Q6DHV7-2] NP_001311295.1, NM_001324366.1 [Q6DHV7-1] NP_001311297.1, NM_001324368.1 [Q6DHV7-2] XP_011519593.1, XM_011521291.2 [Q6DHV7-1] XP_016877453.1, XM_017021964.1 [Q6DHV7-1] XP_016877454.1, XM_017021965.1 [Q6DHV7-1] XP_016877455.1, XM_017021966.1 [Q6DHV7-1] |
Genome annotation databases
Ensembli | ENST00000389651; ENSP00000374302; ENSG00000168803 [Q6DHV7-2] ENST00000422466; ENSP00000398744; ENSG00000168803 [Q6DHV7-3] ENST00000428046; ENSP00000413074; ENSG00000168803 [Q6DHV7-3] ENST00000562188; ENSP00000456242; ENSG00000168803 [Q6DHV7-1] ENST00000610420; ENSP00000478575; ENSG00000168803 [Q6DHV7-2] |
GeneIDi | 161823 |
KEGGi | hsa:161823 |
UCSCi | uc001zrh.5, human [Q6DHV7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK126583 mRNA No translation available. AK298870 mRNA Translation: BAG60990.1 AC009852 Genomic DNA No translation available. AC018924 Genomic DNA No translation available. BC075857 mRNA Translation: AAH75857.1 |
CCDSi | CCDS32214.1 [Q6DHV7-2] CCDS53936.1 [Q6DHV7-3] CCDS81868.1 [Q6DHV7-1] |
RefSeqi | NP_001012987.1, NM_001012969.3 [Q6DHV7-2] NP_001152752.1, NM_001159280.2 [Q6DHV7-3] NP_001311293.1, NM_001324364.1 [Q6DHV7-2] NP_001311294.1, NM_001324365.1 [Q6DHV7-2] NP_001311295.1, NM_001324366.1 [Q6DHV7-1] NP_001311297.1, NM_001324368.1 [Q6DHV7-2] XP_011519593.1, XM_011521291.2 [Q6DHV7-1] XP_016877453.1, XM_017021964.1 [Q6DHV7-1] XP_016877454.1, XM_017021965.1 [Q6DHV7-1] XP_016877455.1, XM_017021966.1 [Q6DHV7-1] |
3D structure databases
SMRi | Q6DHV7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 127803, 4 interactors |
IntActi | Q6DHV7, 17 interactors |
STRINGi | 9606.ENSP00000398744 |
Chemistry databases
ChEMBLi | CHEMBL1795150 |
PTM databases
iPTMneti | Q6DHV7 |
Genetic variation databases
BioMutai | ADAL |
DMDMi | 146286026 |
Proteomic databases
EPDi | Q6DHV7 |
jPOSTi | Q6DHV7 |
MassIVEi | Q6DHV7 |
MaxQBi | Q6DHV7 |
PeptideAtlasi | Q6DHV7 |
PRIDEi | Q6DHV7 |
ProteomicsDBi | 66225 [Q6DHV7-1] 66226 [Q6DHV7-2] 66227 [Q6DHV7-3] |
Protocols and materials databases
Antibodypediai | 23863, 87 antibodies |
DNASUi | 161823 |
Genome annotation databases
Ensembli | ENST00000389651; ENSP00000374302; ENSG00000168803 [Q6DHV7-2] ENST00000422466; ENSP00000398744; ENSG00000168803 [Q6DHV7-3] ENST00000428046; ENSP00000413074; ENSG00000168803 [Q6DHV7-3] ENST00000562188; ENSP00000456242; ENSG00000168803 [Q6DHV7-1] ENST00000610420; ENSP00000478575; ENSG00000168803 [Q6DHV7-2] |
GeneIDi | 161823 |
KEGGi | hsa:161823 |
UCSCi | uc001zrh.5, human [Q6DHV7-1] |
Organism-specific databases
CTDi | 161823 |
GeneCardsi | ADAL |
HGNCi | HGNC:31853, ADAL |
HPAi | ENSG00000168803, Low tissue specificity |
neXtProti | NX_Q6DHV7 |
OpenTargetsi | ENSG00000168803 |
PharmGKBi | PA142672643 |
VEuPathDBi | HostDB:ENSG00000168803.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1097, Eukaryota |
GeneTreei | ENSGT00950000183113 |
HOGENOMi | CLU_039228_6_0_1 |
InParanoidi | Q6DHV7 |
OMAi | RPQFKPY |
PhylomeDBi | Q6DHV7 |
TreeFami | TF314270 |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000168803-MONOMER |
PathwayCommonsi | Q6DHV7 |
Reactomei | R-HSA-2161541, Abacavir metabolism R-HSA-74217, Purine salvage |
Miscellaneous databases
BioGRID-ORCSi | 161823, 6 hits in 992 CRISPR screens |
ChiTaRSi | ADAL, human |
GenomeRNAii | 161823 |
Pharosi | Q6DHV7, Tbio |
PROi | PR:Q6DHV7 |
RNActi | Q6DHV7, protein |
Gene expression databases
Bgeei | ENSG00000168803, Expressed in cardiac muscle of right atrium and 180 other tissues |
ExpressionAtlasi | Q6DHV7, baseline and differential |
Genevisiblei | Q6DHV7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR001365, A/AMP_deaminase_dom IPR032466, Metal_Hydrolase |
Pfami | View protein in Pfam PF00962, A_deaminase, 1 hit |
SUPFAMi | SSF51556, SSF51556, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADAL_HUMAN | |
Accessioni | Q6DHV7Primary (citable) accession number: Q6DHV7 Secondary accession number(s): A6NHZ3, B4DQM8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | May 1, 2007 | |
Last modified: | April 7, 2021 | |
This is version 131 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - SIMILARITY comments
Index of protein domains and families