UniProtKB - Q6DHR8 (NGBR_DANRE)
Dehydrodolichyl diphosphate synthase complex subunit nus1
nus1
Functioni
With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Adds multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol. Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator.
By similarityMiscellaneous
Catalytic activityi
- (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = di-trans,poly-cis-polyprenyl diphosphate + n diphosphateBy similarityEC:2.5.1.87By similarity
Cofactori
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.CuratedView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
GO - Biological processi
- dolichol biosynthetic process Source: GO_Central
- protein glycosylation Source: GO_Central
- sprouting angiogenesis Source: ZFIN
Keywordsi
Molecular function | Receptor, Transferase |
Ligand | Magnesium |
Enzyme and pathway databases
Reactomei | R-DRE-446199, Synthesis of Dolichyl-phosphate |
UniPathwayi | UPA00378 |
Names & Taxonomyi
Protein namesi | Recommended name: Dehydrodolichyl diphosphate synthase complex subunit nus1Curated (EC:2.5.1.87By similarity)Alternative name(s): Di-trans,poly-cis-decaprenylcistransferaseCurated Nogo-B receptorBy similarity Short name: NgBRBy similarity Nuclear undecaprenyl pyrophosphate synthase 1 homologCurated |
Gene namesi | Name:nus1 Synonyms:ngbr ORF Names:si:ch211-102b16.1, zgc:92136 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-040718-48, nus1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- dehydrodolichyl diphosphate synthase complex Source: GO_Central
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1 – 23 | Helical; Name=1By similarityAdd BLAST | 23 | |
Transmembranei | 33 – 49 | Helical; Name=2By similarityAdd BLAST | 17 | |
Transmembranei | 95 – 113 | Helical; Name=3By similarityAdd BLAST | 19 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000273169 | 1 – 274 | Dehydrodolichyl diphosphate synthase complex subunit nus1Add BLAST | 274 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 122 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q6DHR8 |
Expressioni
Gene expression databases
Bgeei | ENSDARG00000027813, Expressed in blastula and 37 other tissues |
ExpressionAtlasi | Q6DHR8, baseline and differential |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2818, Eukaryota |
GeneTreei | ENSGT00390000003223 |
HOGENOMi | CLU_051870_2_1_1 |
InParanoidi | Q6DHR8 |
OMAi | TEIFCLQ |
OrthoDBi | 1448374at2759 |
PhylomeDBi | Q6DHR8 |
TreeFami | TF332448 |
Family and domain databases
Gene3Di | 3.40.1180.10, 1 hit |
InterProi | View protein in InterPro IPR038887, Nus1/NgBR IPR001441, UPP_synth-like IPR036424, UPP_synth-like_sf |
PANTHERi | PTHR21528, PTHR21528, 2 hits |
Pfami | View protein in Pfam PF01255, Prenyltransf, 1 hit |
SUPFAMi | SSF64005, SSF64005, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASLYEMVWR FLHALLYLQR AIVAWFRVHI WRWKLAVVDL LLPLALGFHN
60 70 80 90 100
QKKTGPKGTR TSRRVRWGAD GRTLEKLPLH VGLLVTEEEI HYTDIANLVV
110 120 130 140 150
WCMAVGISYV SVYDNQGVFK RNNSRLMEEI LKQQQELLGM GSSKYSVEIL
160 170 180 190 200
KNGTNKQEHQ VLSCQSMVKV LSPDDGRLSI VQAAQQLCRA VEQKEKTSKD
210 220 230 240 250
INVSVLDSLL KESKNIPDPD LVLKFGTVQS TLGFLPWHIR LTEIISMPSH
260 270
IDASYDDLYD ALQRFAGCEQ RLGK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1R7Y1 | F1R7Y1_DANRE | Ditrans,polycis-polyprenyl diphosph... | nus1 | 212 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_034839 | 245 – 274 | ISMPS…QRLGK → MYMFLGFKGTMEL in isoform 2. CuratedAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU138502 Genomic DNA Translation: CAQ13476.1 CU138502 Genomic DNA Translation: CAQ13477.1 Sequence problems. BC075899 mRNA Translation: AAH75899.1 |
RefSeqi | NP_001002356.1, NM_001002356.1 [Q6DHR8-1] |
Genome annotation databases
Ensembli | ENSDART00000034054; ENSDARP00000034808; ENSDARG00000027813 [Q6DHR8-1] |
GeneIDi | 436629 |
KEGGi | dre:436629 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU138502 Genomic DNA Translation: CAQ13476.1 CU138502 Genomic DNA Translation: CAQ13477.1 Sequence problems. BC075899 mRNA Translation: AAH75899.1 |
RefSeqi | NP_001002356.1, NM_001002356.1 [Q6DHR8-1] |
3D structure databases
SMRi | Q6DHR8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000034808 |
Proteomic databases
PaxDbi | Q6DHR8 |
Genome annotation databases
Ensembli | ENSDART00000034054; ENSDARP00000034808; ENSDARG00000027813 [Q6DHR8-1] |
GeneIDi | 436629 |
KEGGi | dre:436629 |
Organism-specific databases
CTDi | 116150 |
ZFINi | ZDB-GENE-040718-48, nus1 |
Phylogenomic databases
eggNOGi | KOG2818, Eukaryota |
GeneTreei | ENSGT00390000003223 |
HOGENOMi | CLU_051870_2_1_1 |
InParanoidi | Q6DHR8 |
OMAi | TEIFCLQ |
OrthoDBi | 1448374at2759 |
PhylomeDBi | Q6DHR8 |
TreeFami | TF332448 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-DRE-446199, Synthesis of Dolichyl-phosphate |
Miscellaneous databases
PROi | PR:Q6DHR8 |
Gene expression databases
Bgeei | ENSDARG00000027813, Expressed in blastula and 37 other tissues |
ExpressionAtlasi | Q6DHR8, baseline and differential |
Family and domain databases
Gene3Di | 3.40.1180.10, 1 hit |
InterProi | View protein in InterPro IPR038887, Nus1/NgBR IPR001441, UPP_synth-like IPR036424, UPP_synth-like_sf |
PANTHERi | PTHR21528, PTHR21528, 2 hits |
Pfami | View protein in Pfam PF01255, Prenyltransf, 1 hit |
SUPFAMi | SSF64005, SSF64005, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NGBR_DANRE | |
Accessioni | Q6DHR8Primary (citable) accession number: Q6DHR8 Secondary accession number(s): B0V3G6, B0V3G7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2007 |
Last sequence update: | August 16, 2004 | |
Last modified: | February 23, 2022 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families