UniProtKB - Q6D0C3 (SYI_PECAS)
Protein
Isoleucine--tRNA ligase
Gene
ileS
Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Functioni
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation
Catalytic activityi
- EC:6.1.1.5UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 561 | Aminoacyl-adenylateUniRule annotation | 1 | |
Binding sitei | 605 | ATPUniRule annotation | 1 | |
Metal bindingi | 900 | ZincUniRule annotation | 1 | |
Metal bindingi | 903 | ZincUniRule annotation | 1 | |
Metal bindingi | 920 | ZincUniRule annotation | 1 | |
Metal bindingi | 923 | ZincUniRule annotation | 1 |
GO - Molecular functioni
- aminoacyl-tRNA editing activity Source: InterPro
- ATP binding Source: UniProtKB-UniRule
- isoleucine-tRNA ligase activity Source: UniProtKB-UniRule
- tRNA binding Source: InterPro
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- isoleucyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | PATR218491:G1G3T-3976-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)Alternative name(s): Isoleucyl-tRNA synthetaseUniRule annotation Short name: IleRSUniRule annotation |
Gene namesi | Name:ileSUniRule annotation Ordered Locus Names:ECA3876 |
Organismi | Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica) |
Taxonomic identifieri | 218491 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Pectobacteriaceae › Pectobacterium › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098383 | 1 – 937 | Isoleucine--tRNA ligaseAdd BLAST | 937 |
Proteomic databases
PRIDEi | Q6D0C3 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationProtein-protein interaction databases
STRINGi | 218491.ECA3876 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 58 – 68 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 602 – 606 | 'KMSKS' region | 5 |
Domaini
IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105C07 Bacteria COG0060 LUCA |
HOGENOMi | HOG000246402 |
KOi | K01870 |
OMAi | HLGTAWN |
OrthoDBi | 32262at2 |
Family and domain databases
CDDi | cd07960 Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.740.10, 1 hit |
HAMAPi | MF_02002 Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412 aa-tRNA-synth_I_CS IPR002300 aa-tRNA-synth_Ia IPR033708 Anticodon_Ile_BEm IPR002301 Ile-tRNA-ligase IPR023585 Ile-tRNA-ligase_type1 IPR013155 M/V/L/I-tRNA-synth_anticd-bd IPR014729 Rossmann-like_a/b/a_fold IPR009080 tRNAsynth_Ia_anticodon-bd IPR009008 Val/Leu/Ile-tRNA-synth_edit IPR010663 Znf_FPG/IleRS |
Pfami | View protein in Pfam PF08264 Anticodon_1, 1 hit PF00133 tRNA-synt_1, 1 hit PF06827 zf-FPG_IleRS, 1 hit |
PRINTSi | PR00984 TRNASYNTHILE |
SUPFAMi | SSF47323 SSF47323, 1 hit SSF50677 SSF50677, 1 hit |
TIGRFAMsi | TIGR00392 ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178 AA_TRNA_LIGASE_I, 1 hit |
i Sequence
Sequence statusi: Complete.
Q6D0C3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDYKTTLNL PETGFPMRGD LAKREPDMLK RWYEQDLYGI IRNAKKGKKT
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKGLSGYDSP YVPGWDCHGL
110 120 130 140 150
PIELKVEQLI GKPGEKVSAA EFRAECRKYA AEQVAGQKAD FIRLGVLGDW
160 170 180 190 200
DRPYLTMDFK TEANIIRALG RIIENGHLHK GAKPVHWCAD CGSALAEAEV
210 220 230 240 250
EYYDKTSPSI DVTFNASDVA AVLAKFGVSR VDGPISLVIW TTTPWTLPAN
260 270 280 290 300
RAISLNAEFD YQLVQIDGQA LILAADLVES VMKRVGVTQW IVLGDCKGAD
310 320 330 340 350
LELLRFKHPF LGFDVPAILG DHVTLDAGTG AVHTAGGHGP DDYVISQKYN
360 370 380 390 400
LEIANPVGPN GCYLSGTYPE LDGKFVFKAN DLIVEILREK SMLLHVEKLQ
410 420 430 440 450
HSYPCCWRHK SPIIFRATPQ WFVSMDQKGL RKQSLSEIKD VQWIPDWGQA
460 470 480 490 500
RIEAMVANRP DWCISRQRTW GVPMSLFVHK ETEELHPRTA ELIEAVAKRV
510 520 530 540 550
EADGIQAWWD LDPADVLGAD ADNYVKVPDT LDVWFDSGST HASVVDVRPE
560 570 580 590 600
FGGHEADMYL EGSDQHRGWF MSSLMISTAI KGKAPYRQVL THGFTVDGQG
610 620 630 640 650
RKMSKSIGNT VSPQDVMNKL GADILRLWIG STDYSGEIAV SDEILKRSAD
660 670 680 690 700
AYRRIRNTAR FLLANLNGFD PQKDSVKPED MVVLDRWAVG CAKAAQDEIL
710 720 730 740 750
EAYESYDFHR VVQRLMQFCS IEMGSFYLDI IKDRQYTAKG DSVARRSCQT
760 770 780 790 800
ALYHISEALV RWMAPIMSFT ADELWNYLPG ERAQYVFTEE WYDGLFALDA
810 820 830 840 850
SETMNDAFWA DVLKVRSEVN KVIEQARNDK RIGGSLEASV TLYADANLAD
860 870 880 890 900
KLNQLRQELH FALLTSKARV ERYENAPDSA QATELTGLKI ALSEAEGHKC
910 920 930
PRCWHYETDI GSNADHPEVC GRCATNVGGN GEERKFV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX950851 Genomic DNA Translation: CAG76774.1 |
RefSeqi | WP_011095374.1, NC_004547.2 |
Genome annotation databases
EnsemblBacteriai | CAG76774; CAG76774; ECA3876 |
KEGGi | eca:ECA3876 |
PATRICi | fig|218491.5.peg.3932 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX950851 Genomic DNA Translation: CAG76774.1 |
RefSeqi | WP_011095374.1, NC_004547.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 218491.ECA3876 |
Proteomic databases
PRIDEi | Q6D0C3 |
Genome annotation databases
EnsemblBacteriai | CAG76774; CAG76774; ECA3876 |
KEGGi | eca:ECA3876 |
PATRICi | fig|218491.5.peg.3932 |
Phylogenomic databases
eggNOGi | ENOG4105C07 Bacteria COG0060 LUCA |
HOGENOMi | HOG000246402 |
KOi | K01870 |
OMAi | HLGTAWN |
OrthoDBi | 32262at2 |
Enzyme and pathway databases
BioCyci | PATR218491:G1G3T-3976-MONOMER |
Family and domain databases
CDDi | cd07960 Anticodon_Ia_Ile_BEm, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.740.10, 1 hit |
HAMAPi | MF_02002 Ile_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412 aa-tRNA-synth_I_CS IPR002300 aa-tRNA-synth_Ia IPR033708 Anticodon_Ile_BEm IPR002301 Ile-tRNA-ligase IPR023585 Ile-tRNA-ligase_type1 IPR013155 M/V/L/I-tRNA-synth_anticd-bd IPR014729 Rossmann-like_a/b/a_fold IPR009080 tRNAsynth_Ia_anticodon-bd IPR009008 Val/Leu/Ile-tRNA-synth_edit IPR010663 Znf_FPG/IleRS |
Pfami | View protein in Pfam PF08264 Anticodon_1, 1 hit PF00133 tRNA-synt_1, 1 hit PF06827 zf-FPG_IleRS, 1 hit |
PRINTSi | PR00984 TRNASYNTHILE |
SUPFAMi | SSF47323 SSF47323, 1 hit SSF50677 SSF50677, 1 hit |
TIGRFAMsi | TIGR00392 ileS, 1 hit |
PROSITEi | View protein in PROSITE PS00178 AA_TRNA_LIGASE_I, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYI_PECAS | |
Accessioni | Q6D0C3Primary (citable) accession number: Q6D0C3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | August 16, 2004 | |
Last modified: | October 16, 2019 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families