Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thiamine thiazole synthase

Gene

THI4

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73Substrate; via amide nitrogenUniRule annotation1
Binding sitei102Substrate; via amide nitrogenUniRule annotation1
Binding sitei167Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei205SubstrateUniRule annotation1
Binding sitei235SubstrateUniRule annotation1
Binding sitei289Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:THI4UniRule annotation
ORF Names:KLLA0_A00198gImported
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)Imported
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2032,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-203 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Protein-protein interaction databases

STRINGi284590.XP_451008.1

Structurei

3D structure databases

ProteinModelPortaliQ6CYI1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 95Substrate bindingUniRule annotation2
Regioni299 – 301Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2960 Eukaryota
COG1635 LUCA
HOGENOMiHOG000106048
InParanoidiQ6CYI1
KOiK03146
OMAiMWGGGMM
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

Q6CYI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTQTQTQN KLSVQSQSVE HCLSDVVKQD DWSDFAFAPI REATVSRAMT
60 70 80 90 100
SRYFKDLDKF AVSDVVIIGA GSSGLSAAYV IAKNRPDLKI AIIEANVAPG
110 120 130 140 150
GGCWLGGQLF SAMIMRKPAD KFLDELNIPY EDEGHYVVVK HAALFMSTVL
160 170 180 190 200
SEVLKFPNVK MFNATAVEDL VTRPADDNSQ HVNVAGVVTN WTLVTQNHDT
210 220 230 240 250
QSCMDPNVIE LSGYKDNGER DLSQKHGVIL SCCGHDGPFG AFTVKRMASI
260 270 280 290 300
DSSKSYAGMK GLDMNRAEDA VVKNAGAYDK VGSVYFAGME VAEHAGLNRM
310 320
GPTFGAMAVS GIKAAEDILK HFAA
Length:324
Mass (Da):34,971
Last modified:August 16, 2004 - v1
Checksum:i1D0AFE8F4A870A7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382121 Genomic DNA Translation: CAH02596.1
RefSeqiXP_451008.1, XM_451008.1

Genome annotation databases

EnsemblFungiiCAH02596; CAH02596; KLLA0_A00198g
GeneIDi2896472
KEGGikla:KLLA0A00198g

Similar proteinsi

Entry informationi

Entry nameiQ6CYI1_KLULA
AccessioniPrimary (citable) accession number: Q6CYI1
Entry historyiIntegrated into UniProtKB/TrEMBL: August 16, 2004
Last sequence update: August 16, 2004
Last modified: March 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health