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Protein

DNA polymerase epsilon catalytic subunit A

Gene

POL2

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA polymerase II participates in chromosomal DNA replication.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi2066ZincBy similarity1
Metal bindingi2069ZincBy similarity1
Metal bindingi2090ZincBy similarity1
Metal bindingi2093ZincBy similarity1
Metal bindingi2124Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi2127Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi2139Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi2141Iron-sulfur (4Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri2066 – 2093CysA-typeAdd BLAST28

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon catalytic subunit A (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit A
Gene namesi
Name:POL2
Ordered Locus Names:YALI0E26367g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
Proteomesi
  • UP000001300 Componenti: Chromosome E

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464661 – 2183DNA polymerase epsilon catalytic subunit AAdd BLAST2183

Interactioni

Subunit structurei

Heterotetramer. Consists of 4 subunits: POL2, DPB2, DPB3 and DPB4 (By similarity).By similarity

Protein-protein interaction databases

STRINGi4952.XP_504422.1

Structurei

3D structure databases

ProteinModelPortaliQ6C4J0
SMRiQ6C4J0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi2124 – 2141CysB motifAdd BLAST18

Domaini

The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C.By similarity
The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.By similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri2066 – 2093CysA-typeAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000196287
InParanoidiQ6C4J0
KOiK02324
OMAiIHSKEIF
OrthoDBiEOG092C00WD

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR013697 DNA_pol_e_suA_C
IPR029703 POL2
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10670 PTHR10670, 1 hit
PfamiView protein in Pfam
PF00136 DNA_pol_B, 1 hit
PF03104 DNA_pol_B_exo1, 1 hit
PF08490 DUF1744, 1 hit
SMARTiView protein in SMART
SM01159 DUF1744, 1 hit
SM00486 POLBc, 1 hit
SUPFAMiSSF53098 SSF53098, 2 hits

Sequencei

Sequence statusi: Complete.

Q6C4J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRPFNGNNT HVVKQRSDEF ERRRLERIEQ LGQKFAAVER RDAIDDRMGF
60 70 80 90 100
TRFSGDEKRV GWLVNMHETL LQSETAERGL AAVDYYFYDE EGGNFKSTVV
110 120 130 140 150
FRPYFFVICK PHTEHAVKDL MEKMFERVLA STEIVTKEDL NLTNHLTGKK
160 170 180 190 200
RKAVKLEFHN SEDLSSTRFT LSKIVDRQTQ QTEQHLNIYE LEGDVDTSTD
210 220 230 240 250
VESSITGIKE FDVPFETRVA IDLDIRVGNW YEVTKENDTA VLKHMVEREY
260 270 280 290 300
RADPVVMAYD IETEKAPLRF PDSAVDRIMM ISYMIDGEGF LITNREMVSE
310 320 330 340 350
DIEDFEYTPK PEFPGNFTIF NEPNEKAVLE KWFEHIRDVC PTVMTSYNGD
360 370 380 390 400
FFDFPFIDKR TAFHGMNLYD EIGWKKVEEE RYECSYCVHM DCLNWVKRDS
410 420 430 440 450
YLPQGSQGLK AVTKVKLSYD PKELDPEKMT PYARDHPQIL AEYSVSDAVA
460 470 480 490 500
TYYLYMKYVH PFIFSLCCII PLNPDAVLRK GTGTLCEMLL MVKAYEGRII
510 520 530 540 550
LPDKHKPALE RHYKGHLVDN ETYTGGHVES LAAGVFRSDI MVDFDIDTNS
560 570 580 590 600
IDELLVNLDE TLEFCVTVEA GKKSSDFENL DEVRDQIIEQ LQELKSNPKR
610 620 630 640 650
SDYPLIYHVD VASMYPNIMT TNRLQPDSMV DEKDCAVCDY NRPDKTCARE
660 670 680 690 700
LEWARRVDYY PVSKGDVNNL KQGLVEEYSG GRFGNGSSVE ISYDEGMTER
710 720 730 740 750
EKFQKRKGWA GLSGQEQANK LKERVAAFSL KTKARKTDSE TTVQKTIVCQ
760 770 780 790 800
RENPFYVDVV QEFKQRRIDY KTKAKRWNKE AASASDPASR EEAKKMAITN
810 820 830 840 850
DSMQLAHKVI LNSFYGYVMR KGSRWYSMEM AGVTCYTGAK IIKIARQTME
860 870 880 890 900
GLGIPLELDT DGIWCMLPKT FPEKFKCKFK DGSSYELEYP CSIMNYLVHR
910 920 930 940 950
DFTNHQYQKL NPETGKYDTH SENSIFFELD GPYKCMMMPT STDKGKGLKK
960 970 980 990 1000
RYVVFDDRNK IVELKGFEVK RRGELSLIKK FQSQLWDTFL EGSNLVECYA
1010 1020 1030 1040 1050
AQARVAEAWL AVIDSRGKNL SDEELIDLVC ENKSMSKPVH EYGSQKSTAL
1060 1070 1080 1090 1100
TTARRLAEIM GDSILTGGKL STKYIIAVRT KNSMPVPEAK KGSDEDSSTA
1110 1120 1130 1140 1150
DRAIPTLVFE TESLDEKLRF LKRWMGPRWE STDPRDVIDW MYYRERLATT
1160 1170 1180 1190 1200
INKLVVIPAI LLGLKNPVRG CDPPEWASDI IKQRDNPIKQ STLSSYFVKG
1210 1220 1230 1240 1250
KLPTPEPILS EEDEVMEIQV GDIESIGTTP SPSRPPGVPA RVVVSKRVRK
1260 1270 1280 1290 1300
TVEETDKSTP SLPHKAPDPF ADYAAYIKYA KVKWKHQKAQ RDRRAHLFGE
1310 1320 1330 1340 1350
SDNGIASSWV SKNAHLAQDA WHLVSIHAAE KPGQVEASVI IGDKMQRVQI
1360 1370 1380 1390 1400
NVPRKVYVGS REPLKWDKFT DVTNAMAVAE EEQPKYLYRA VMSEDMYQTE
1410 1420 1430 1440 1450
MTNPESPLKD PHVTKIYEAD VSPDSRALIE LGSTFHLDAS TPGILSKGFT
1460 1470 1480 1490 1500
HGFEAKWFKS GDSRSYLQNS GLSYAHIVHV VSSAVEIFVI TPTWDAPALV
1510 1520 1530 1540 1550
FVHQSSSSEK LPDISKEYAR LRRGERYKQN MDDCTVFSFP DTMQYEVEYC
1560 1570 1580 1590 1600
SNHRRMLQKV SKACDSLSGS RNGQLVFALH SPDRNVDEKV AALARIPCIR
1610 1620 1630 1640 1650
LRPVPPSTAA VGWQRDLVRR LIASFLSHGA NISYLVEMAR YAKMPLCHVQ
1660 1670 1680 1690 1700
DTRDVIDVSY ARRLIQNSVV LWWSAALSTG GALTDQAEVP VANNPGLYTN
1710 1720 1730 1740 1750
ICFEIKIEHL VLNALLQSAV IQDTEGDIHN ELLSNAFNPH ALKTLKMVVK
1760 1770 1780 1790 1800
EWWEHGDKKR PPQDLLDNFT VWVYAPESRL FSPQLLYHTQ NLTKKTFLYL
1810 1820 1830 1840 1850
AQECRKHQAQ LVYADQHKLV LQTEKTELQL VYSYSNYIVR QIRTHPILKY
1860 1870 1880 1890 1900
VSVDITRYWD TFLWMDKWNY GGYSSDVIVD PSKQSESLLM HWHIANFLPE
1910 1920 1930 1940 1950
TLQQEFSKWV QEYIHLLRLR KYPENRTTDV DDMPSDTPML TDGDKDDAAL
1960 1970 1980 1990 2000
LSDKFFGKGV VSYLHPKLVR RVKLLAQKLS DVLSSGDSID AFKFPVLPGS
2010 2020 2030 2040 2050
MIQPETNPVV EFAKYVCHIY GINAKANFEV QILLRDLLNI FDINEFSEEG
2060 2070 2080 2090 2100
TFRDPSMSLK LTNMMCFKCK NPCDLDLCKD SCCTKSGFRC PLCNSLYDMV
2110 2120 2130 2140 2150
IVEQRLVGQL QRMILEYETQ DFRCDKCRRV KEYELTEFCP CSGSWVTIMS
2160 2170 2180
AEDVHKELSI YDRCARWFEL KMLGSFLRQL GYV
Length:2,183
Mass (Da):250,638
Last modified:August 16, 2004 - v1
Checksum:i969273FC9800ED8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382131 Genomic DNA Translation: CAG80023.1
RefSeqiXP_504422.1, XM_504422.1

Genome annotation databases

EnsemblFungiiCAG80023; CAG80023; YALI0_E26367g
GeneIDi2912373
KEGGiyli:YALI0E26367g

Similar proteinsi

Entry informationi

Entry nameiDPOE_YARLI
AccessioniPrimary (citable) accession number: Q6C4J0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: August 16, 2004
Last modified: February 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health