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Protein

Thiamine thiazole synthase

Gene

THI4

Organism
Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei82Substrate; via amide nitrogenUniRule annotation1
Binding sitei111Substrate; via amide nitrogenUniRule annotation1
Binding sitei176Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei213SubstrateUniRule annotation1
Binding sitei228SubstrateUniRule annotation1
Binding sitei279Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:THI4UniRule annotation
Ordered Locus Names:DEHA2F16654gImported
OrganismiDebaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii)Imported
Taxonomic identifieri284592 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeDebaryomyces
Proteomesi
  • UP000000599 Componenti: Chromosome F

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2112,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-211 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 104Substrate bindingUniRule annotation2
Regioni289 – 291Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiQ6BL37
KOiK03146
OMAiMWGGGMM
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

Q6BL37-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPPTMIETS VRHEFSPIVT EDRVVRMSSD AQNSDVKFAD WDKFKFTPIR
60 70 80 90 100
ESTVSRAMTS RYFADLDKYA ESDVVIIGAG SAGLSAAYIL AKNRPELKIA
110 120 130 140 150
IIEAGVSPGG GCWLGGQLFS AMILRKPAHL FLEELGLEYE DEGDYVVVKH
160 170 180 190 200
AAFFMSTLLS KVLSFPNVKL FNATAVEDLI TRRDETTGDL RICGVVTNWT
210 220 230 240 250
LVSLNHDTQS CMDPNTINAN VILSASGHDG PFGAGSAKRL DKLGCIELGH
260 270 280 290 300
MRGLDMNSAE DAIVKGTREV TPGLVVTGME LAEVDGSNRM GPTFGAMALS
310 320
GVKAAEAVLN VYDLRKKQNN P
Length:321
Mass (Da):34,724
Last modified:November 4, 2008 - v2
Checksum:i7490B0E93204F1C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382138 Genomic DNA Translation: CAG89466.2
RefSeqiXP_461084.2, XM_461084.1

Genome annotation databases

EnsemblFungiiCAG89466; CAG89466; DEHA2F16654g
GeneIDi2904109
KEGGidha:DEHA2F16654g

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR382138 Genomic DNA Translation: CAG89466.2
RefSeqiXP_461084.2, XM_461084.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG89466; CAG89466; DEHA2F16654g
GeneIDi2904109
KEGGidha:DEHA2F16654g

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiQ6BL37
KOiK03146
OMAiMWGGGMM
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiQ6BL37_DEBHA
AccessioniPrimary (citable) accession number: Q6BL37
Entry historyiIntegrated into UniProtKB/TrEMBL: August 16, 2004
Last sequence update: November 4, 2008
Last modified: November 7, 2018
This is version 83 of the entry and version 2 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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