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Protein

Sphingosine kinase B

Gene

sgkB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), which probably acts intracellularly as a second messenger perhaps by promoting cell proliferation.

Catalytic activityi

ATP + a sphingoid base = ADP + a sphingoid base 1-phosphate.By similarity

Activity regulationi

Inhibited by N,N,-dimethylsphingosine.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei288ATPPROSITE-ProRule annotation1
Active sitei315Proton donor/acceptorBy similarity1
Binding sitei320ATPPROSITE-ProRule annotation1
Binding sitei461SubstrateBy similarity1
Binding sitei468ATPPROSITE-ProRule annotation1
Binding sitei474ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi257 – 259ATPPROSITE-ProRule annotation3
Nucleotide bindingi345 – 347ATPPROSITE-ProRule annotation3
Nucleotide bindingi734 – 736ATPPROSITE-ProRule annotation3

GO - Molecular functioni

GO - Biological processi

  • dihydrosphingosine-1-P pathway Source: dictyBase
  • positive regulation of asexual reproduction Source: dictyBase
  • sphingolipid metabolic process Source: UniProtKB-KW

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid metabolism, Sphingolipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-1483206 Glycerophospholipid biosynthesis
R-DDI-1660662 Glycosphingolipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine kinase B (EC:2.7.1.91By similarity)
Short name:
SK B
Short name:
SPK B
Gene namesi
Name:sgkB
Synonyms:SK, SPHK
ORF Names:DDB_G0284545
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0284545 sgkB

Pathology & Biotechi

Disruption phenotypei

Cells grow more slowly on solid media and are more sensitive than wild-type to the antitumor agents cisplatin and carboplatin; both effects are exacerbated in sgkA and sgkB double knockout. Exogenous S1P reverses the sensitivity to cisplatin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003282511 – 760Sphingosine kinase BAdd BLAST760

Proteomic databases

PaxDbiQ6B516
PRIDEiQ6B516

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0220131

Structurei

3D structure databases

ProteinModelPortaliQ6B516
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini247 – 383DAGKcPROSITE-ProRule annotationAdd BLAST137

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni313 – 316Substrate bindingBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi46 – 53Poly-Asn8
Compositional biasi91 – 103Poly-AsnAdd BLAST13
Compositional biasi178 – 188Poly-ThrAdd BLAST11
Compositional biasi394 – 402Poly-Thr9
Compositional biasi415 – 442Poly-AsnAdd BLAST28
Compositional biasi556 – 562Poly-Asn7
Compositional biasi563 – 568Poly-Thr6
Compositional biasi582 – 587Poly-Thr6

Phylogenomic databases

eggNOGiKOG1116 Eukaryota
COG1597 LUCA
InParanoidiQ6B516
KOiK04718
OMAiMEPEHAK
PhylomeDBiQ6B516

Family and domain databases

Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
PfamiView protein in Pfam
PF00781 DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00046 DAGKc, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit

Sequencei

Sequence statusi: Complete.

Q6B516-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MENNNNEPAE TVQEKGPKLK NDIDLNDQFK DEKEKKEEIS SSSIENKNNN
60 70 80 90 100
NNNTSTNNVI PNESNNNISN NNVISNENNN TNNNTDNNTD NNNNNNNNNN
110 120 130 140 150
NNNEPVTSFT VNNKSLIEKE PTLFEFNQEQ QIKYKNKSIN SLIIKRSGIV
160 170 180 190 200
IKISQKKSTI IYQDSIIACS ELPDNFDTAT TTTTTTTTLP IKVTLFTCVL
210 220 230 240 250
KKKQLTIDKE QRKRKSYTFQ FKSNQDSLNF YSNIQSTFLN SLPRGNPKNR
260 270 280 290 300
KIRILINPKS GKKESHNIFK EVEQLFKDSG IKMKLTVTME PEHAKKIGFK
310 320 330 340 350
SNIYKYDTVV FISGDGLLHE FINGLLSRED YEDAKKIPLA LIPAGTGNGL
360 370 380 390 400
ANSIGLQDPM SAALAILRGF TKPLDVCIVQ QPTVTTIPVV DNNTVTTTTT
410 420 430 440 450
TTSPTSASPT ITSANNNNNN NNNNNNNNNN NNNNNNNNNN SNITKWCSIL
460 470 480 490 500
SLTWGLVSDV DIESEKYRSL GDLRLIIGAA VRILNLRIYR GKVYFLPAIP
510 520 530 540 550
LDKSQMQSIP KCSFDCNICD SSNSVKVIED LVCNDNDNNN KNKNEEQNEI
560 570 580 590 600
NSTTSNNNNN NNTTTTSTSS STSTSTSTSS LTATTTTAKS TNSLSSSPRS
610 620 630 640 650
DINMSSNSIS KSLDIGTIPS CKVTHNSNLL NESSDSLLSK GWKCIEGEFI
660 670 680 690 700
GVVASTVSHL ASDFISSPNA HLSDGLIDLI FINNRSKLSK ASLLSILTDS
710 720 730 740 750
ATGDHLKSDL IEHHKVKALI LEPSIQKHGI VAIDGERIPY AKTSMENIRG
760
CLNLICRSYH
Length:760
Mass (Da):83,966
Last modified:September 13, 2004 - v1
Checksum:iD505C2167ACB266C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY679519 mRNA Translation: AAT80892.1
AAFI02000066 Genomic DNA Translation: EAL65199.1
RefSeqiXP_638540.1, XM_633448.1

Genome annotation databases

EnsemblProtistsiEAL65199; EAL65199; DDB_G0284545
GeneIDi8624633
KEGGiddi:DDB_G0284545

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY679519 mRNA Translation: AAT80892.1
AAFI02000066 Genomic DNA Translation: EAL65199.1
RefSeqiXP_638540.1, XM_633448.1

3D structure databases

ProteinModelPortaliQ6B516
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0220131

Proteomic databases

PaxDbiQ6B516
PRIDEiQ6B516

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL65199; EAL65199; DDB_G0284545
GeneIDi8624633
KEGGiddi:DDB_G0284545

Organism-specific databases

dictyBaseiDDB_G0284545 sgkB

Phylogenomic databases

eggNOGiKOG1116 Eukaryota
COG1597 LUCA
InParanoidiQ6B516
KOiK04718
OMAiMEPEHAK
PhylomeDBiQ6B516

Enzyme and pathway databases

ReactomeiR-DDI-1483206 Glycerophospholipid biosynthesis
R-DDI-1660662 Glycosphingolipid metabolism

Miscellaneous databases

PROiPR:Q6B516

Family and domain databases

Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
PfamiView protein in Pfam
PF00781 DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00046 DAGKc, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPHKB_DICDI
AccessioniPrimary (citable) accession number: Q6B516
Secondary accession number(s): Q54PJ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: September 13, 2004
Last modified: November 7, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
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Main funding by: National Institutes of Health

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