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Protein

E3 ubiquitin-protein ligase TRIM68

Gene

TRIM68

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity.1 Publication

Miscellaneous

Antibodies against TRIM68 are found in patients with systemic lupus erythematosus (SLE) and primary Sjoegren syndrome.

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 61RING-typePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri93 – 134B box-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • androgen receptor binding Source: UniProtKB
  • histone acetyltransferase binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-877300 Interferon gamma signaling
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM68 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 137
RING-type E3 ubiquitin transferase TRIM68Curated
SSA protein SS-56
Short name:
SS-56
Tripartite motif-containing protein 68
Gene namesi
Name:TRIM68
Synonyms:GC109, RNF137, SS56
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000167333.12
HGNCiHGNC:21161 TRIM68
MIMi613184 gene
neXtProtiNX_Q6AZZ1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55128
OpenTargetsiENSG00000167333
PharmGKBiPA134939870

Polymorphism and mutation databases

BioMutaiTRIM68
DMDMi74748376

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002494371 – 485E3 ubiquitin-protein ligase TRIM68Add BLAST485

Post-translational modificationi

Auto-ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ6AZZ1
MaxQBiQ6AZZ1
PaxDbiQ6AZZ1
PeptideAtlasiQ6AZZ1
PRIDEiQ6AZZ1
ProteomicsDBi66204

PTM databases

iPTMnetiQ6AZZ1
PhosphoSitePlusiQ6AZZ1

Expressioni

Tissue specificityi

Widely expressed. Expressed at high levels in prostate cancer cell lines. Up-regulation could be restricted to androgen-dependent cells.3 Publications

Inductioni

Up-regulated in prostate cancer.

Gene expression databases

BgeeiENSG00000167333 Expressed in 194 organ(s), highest expression level in testis
CleanExiHS_TRIM68
ExpressionAtlasiQ6AZZ1 baseline and differential
GenevisibleiQ6AZZ1 HS

Organism-specific databases

HPAiHPA023455

Interactioni

Subunit structurei

Interacts with AR/androgen receptor (via ligand-binding domain). Interacts with KAT5/TIP60.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi120434, 40 interactors
IntActiQ6AZZ1, 12 interactors
STRINGi9606.ENSP00000300747

Structurei

3D structure databases

ProteinModelPortaliQ6AZZ1
SMRiQ6AZZ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini285 – 481B30.2/SPRYPROSITE-ProRule annotationAdd BLAST197

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili207 – 239Sequence analysisAdd BLAST33

Domaini

The RING domain is essential for ubiquitin E3 ligase activity.1 Publication

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 61RING-typePROSITE-ProRule annotationAdd BLAST46
Zinc fingeri93 – 134B box-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG4111G04 LUCA
GeneTreeiENSGT00760000118893
HOVERGENiHBG001357
InParanoidiQ6AZZ1
KOiK12033
OMAiFSPCYSI
OrthoDBiEOG091G05W2
PhylomeDBiQ6AZZ1
TreeFamiTF338674

Family and domain databases

CDDicd00021 BBOX, 1 hit
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR006574 PRY
IPR003877 SPRY_dom
IPR000315 Znf_B-box
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00336 BBOX, 1 hit
SM00589 PRY, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q6AZZ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDPTALVEAI VEEVACPICM TFLREPMSID CGHSFCHSCL SGLWEIPGES
60 70 80 90 100
QNWGYTCPLC RAPVQPRNLR PNWQLANVVE KVRLLRLHPG MGLKGDLCER
110 120 130 140 150
HGEKLKMFCK EDVLIMCEAC SQSPEHEAHS VVPMEDVAWE YKWELHEALE
160 170 180 190 200
HLKKEQEEAW KLEVGERKRT ATWKIQVETR KQSIVWEFEK YQRLLEKKQP
210 220 230 240 250
PHRQLGAEVA AALASLQREA AETMQKLELN HSELIQQSQV LWRMIAELKE
260 270 280 290 300
RSQRPVRWML QDIQEVLNRS KSWSLQQPEP ISLELKTDCR VLGLREILKT
310 320 330 340 350
YAADVRLDPD TAYSRLIVSE DRKRVHYGDT NQKLPDNPER FYRYNIVLGS
360 370 380 390 400
QCISSGRHYW EVEVGDRSEW GLGVCKQNVD RKEVVYLSPH YGFWVIRLRK
410 420 430 440 450
GNEYRAGTDE YPILSLPVPP RRVGIFVDYE AHDISFYNVT DCGSHIFTFP
460 470 480
RYPFPGRLLP YFSPCYSIGT NNTAPLAICS LDGED
Length:485
Mass (Da):56,259
Last modified:September 13, 2004 - v1
Checksum:iFCB9A6076382E2A5
GO
Isoform 2 (identifier: Q6AZZ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MDP → MQK
     4-282: Missing.

Note: No experimental confirmation available.
Show »
Length:206
Mass (Da):23,798
Checksum:i8D38B44D58737F7B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PP83E9PP83_HUMAN
E3 ubiquitin-protein ligase TRIM68
TRIM68
142Annotation score:
E9PR29E9PR29_HUMAN
E3 ubiquitin-protein ligase TRIM68
TRIM68
198Annotation score:
E9PJJ9E9PJJ9_HUMAN
E3 ubiquitin-protein ligase TRIM68
TRIM68
187Annotation score:

Sequence cautioni

The sequence BAA91569 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB14309 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44W → R in BAG51737 (PubMed:14702039).Curated1
Sequence conflicti109 – 114CKEDVL → RGNSLQ in AAF91075 (PubMed:11597395).Curated6
Sequence conflicti175I → V in AAF91075 (PubMed:11597395).Curated1
Sequence conflicti303A → G in AAL31641 (Ref. 2) Curated1
Sequence conflicti304D → V in BAG62716 (PubMed:14702039).Curated1
Sequence conflicti314S → P in BAB14309 (PubMed:14702039).Curated1
Sequence conflicti376K → Y in AAF91075 (PubMed:11597395).Curated1
Sequence conflicti399R → K in AAF91075 (PubMed:11597395).Curated1
Sequence conflicti411Y → V in AAF91075 (PubMed:11597395).Curated1
Sequence conflicti422R → P in AAF91075 (PubMed:11597395).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027415442C → Y1 PublicationCorresponds to variant dbSNP:rs2231975Ensembl.1
Natural variantiVAR_063007457R → H. Corresponds to variant dbSNP:rs2231976Ensembl.1
Natural variantiVAR_063008466Y → S. Corresponds to variant dbSNP:rs7109316Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0554441 – 3MDP → MQK in isoform 2. 1 Publication3
Alternative sequenceiVSP_0554454 – 282Missing in isoform 2. 1 PublicationAdd BLAST279

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF360739 mRNA Translation: AAL11501.1
AF439153 mRNA Translation: AAL31641.1
AK001231 mRNA Translation: BAA91569.1 Different initiation.
AK022923 mRNA Translation: BAB14309.1 Different initiation.
AK291166 mRNA Translation: BAF83855.1
AK301120 mRNA Translation: BAG62716.1
AK056523 mRNA Translation: BAG51737.1
AC090719 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW68835.1
BC075058 mRNA Translation: AAH75058.1
BC109063 mRNA Translation: AAI09064.1
AY005802 mRNA Translation: AAF91075.1
CCDSiCCDS31356.1 [Q6AZZ1-1]
RefSeqiNP_001291425.1, NM_001304496.1
NP_060543.5, NM_018073.7 [Q6AZZ1-1]
UniGeneiHs.523438
Hs.738828

Genome annotation databases

EnsembliENST00000300747; ENSP00000300747; ENSG00000167333 [Q6AZZ1-1]
GeneIDi55128
KEGGihsa:55128
UCSCiuc001lzf.2 human [Q6AZZ1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF360739 mRNA Translation: AAL11501.1
AF439153 mRNA Translation: AAL31641.1
AK001231 mRNA Translation: BAA91569.1 Different initiation.
AK022923 mRNA Translation: BAB14309.1 Different initiation.
AK291166 mRNA Translation: BAF83855.1
AK301120 mRNA Translation: BAG62716.1
AK056523 mRNA Translation: BAG51737.1
AC090719 Genomic DNA No translation available.
CH471064 Genomic DNA Translation: EAW68835.1
BC075058 mRNA Translation: AAH75058.1
BC109063 mRNA Translation: AAI09064.1
AY005802 mRNA Translation: AAF91075.1
CCDSiCCDS31356.1 [Q6AZZ1-1]
RefSeqiNP_001291425.1, NM_001304496.1
NP_060543.5, NM_018073.7 [Q6AZZ1-1]
UniGeneiHs.523438
Hs.738828

3D structure databases

ProteinModelPortaliQ6AZZ1
SMRiQ6AZZ1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120434, 40 interactors
IntActiQ6AZZ1, 12 interactors
STRINGi9606.ENSP00000300747

PTM databases

iPTMnetiQ6AZZ1
PhosphoSitePlusiQ6AZZ1

Polymorphism and mutation databases

BioMutaiTRIM68
DMDMi74748376

Proteomic databases

EPDiQ6AZZ1
MaxQBiQ6AZZ1
PaxDbiQ6AZZ1
PeptideAtlasiQ6AZZ1
PRIDEiQ6AZZ1
ProteomicsDBi66204

Protocols and materials databases

DNASUi55128
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300747; ENSP00000300747; ENSG00000167333 [Q6AZZ1-1]
GeneIDi55128
KEGGihsa:55128
UCSCiuc001lzf.2 human [Q6AZZ1-1]

Organism-specific databases

CTDi55128
DisGeNETi55128
EuPathDBiHostDB:ENSG00000167333.12
GeneCardsiTRIM68
HGNCiHGNC:21161 TRIM68
HPAiHPA023455
MIMi613184 gene
neXtProtiNX_Q6AZZ1
OpenTargetsiENSG00000167333
PharmGKBiPA134939870
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG4111G04 LUCA
GeneTreeiENSGT00760000118893
HOVERGENiHBG001357
InParanoidiQ6AZZ1
KOiK12033
OMAiFSPCYSI
OrthoDBiEOG091G05W2
PhylomeDBiQ6AZZ1
TreeFamiTF338674

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-877300 Interferon gamma signaling

Miscellaneous databases

ChiTaRSiTRIM68 human
GeneWikiiTRIM68
GenomeRNAii55128
PROiPR:Q6AZZ1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167333 Expressed in 194 organ(s), highest expression level in testis
CleanExiHS_TRIM68
ExpressionAtlasiQ6AZZ1 baseline and differential
GenevisibleiQ6AZZ1 HS

Family and domain databases

CDDicd00021 BBOX, 1 hit
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR006574 PRY
IPR003877 SPRY_dom
IPR000315 Znf_B-box
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00336 BBOX, 1 hit
SM00589 PRY, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRI68_HUMAN
AccessioniPrimary (citable) accession number: Q6AZZ1
Secondary accession number(s): A6NI19
, A8K551, B3KPM5, B4DVK4, Q8WZ70, Q96LE5, Q96PF7, Q9H9C2, Q9NW18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 13, 2004
Last modified: September 12, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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