UniProtKB - Q6AZA0 (THIL_DANRE)
Protein
Acetyl-CoA acetyltransferase, mitochondrial
Gene
acat1
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.By similarity
Catalytic activityi
- EC:2.3.1.9PROSITE-ProRule annotation
: fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 119 | Acyl-thioester intermediateBy similarity | 1 | |
Metal bindingi | 212 | PotassiumBy similarity | 1 | |
Binding sitei | 212 | Coenzyme ABy similarity | 1 | |
Binding sitei | 256 | Coenzyme ABy similarity | 1 | |
Metal bindingi | 273 | Potassium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 274 | Potassium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 276 | Potassium; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 277 | Coenzyme ABy similarity | 1 | |
Metal bindingi | 374 | Potassium; via carbonyl oxygenBy similarity | 1 | |
Sitei | 378 | Increases nucleophilicity of active site CysBy similarity | 1 | |
Active sitei | 406 | Proton donor/acceptorBy similarity | 1 |
GO - Molecular functioni
- acetyl-CoA C-acetyltransferase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- fatty acid beta-oxidation Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | Metal-binding, Potassium |
Enzyme and pathway databases
Reactomei | R-DRE-70895, Branched-chain amino acid catabolism R-DRE-77108, Utilization of Ketone Bodies R-DRE-77111, Synthesis of Ketone Bodies |
UniPathwayi | UPA00659 |
Names & Taxonomyi
Protein namesi | Recommended name: Acetyl-CoA acetyltransferase, mitochondrial (EC:2.3.1.9By similarity)Alternative name(s): Acetoacetyl-CoA thiolase |
Gene namesi | Name:acat1 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-040808-68, acat1 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Mitochondrion
- mitochondrion Source: GO_Central
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 26 | MitochondrionBy similarityAdd BLAST | 26 | |
ChainiPRO_0000356275 | 27 – 420 | Acetyl-CoA acetyltransferase, mitochondrialAdd BLAST | 394 |
Proteomic databases
PaxDbi | Q6AZA0 |
PeptideAtlasi | Q6AZA0 |
PRIDEi | Q6AZA0 |
Expressioni
Gene expression databases
Bgeei | ENSDARG00000045888, Expressed in heart and 33 other tissues |
ExpressionAtlasi | Q6AZA0, baseline and differential |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 7955.ENSDARP00000067447 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 251 – 253 | Coenzyme A bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1390, Eukaryota |
GeneTreei | ENSGT01020000230461 |
HOGENOMi | CLU_031026_0_1_1 |
InParanoidi | Q6AZA0 |
OrthoDBi | 797770at2759 |
PhylomeDBi | Q6AZA0 |
TreeFami | TF300650 |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 2 hits |
InterProi | View protein in InterPro IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q6AZA0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSRALYSTR SQLCRHLAHK YLSRSYSTRP SLNEVVIVSA VRTPIGSFKG
60 70 80 90 100
SLSTLPATKL GSIAIKGAID KAGIPVEEVK EVYMGNVLQA GEGQAPTRQA
110 120 130 140 150
LLGAGLPLST PATTINKVCA SGMKSIMLAS QSLMCGHQDV MVAGGMESMS
160 170 180 190 200
QVPYIMAREA PPYGGVKMED LIVKDGLTDV YNKFHMGNCA ENTAKNSSIS
210 220 230 240 250
REEQDAFAIK SYTLSKAAWE SGILAKEVVP VSIPQRGKPD VVVKEDEEYR
260 270 280 290 300
KVDFSKVPKL KAVFLKENGT VTAANASTLN DGAAALVLMT ADAAQRLNVT
310 320 330 340 350
PLAKIVAFAD AAVAPIDFPI APAFAVPKVL KAAGIKKEDI AMWEINEAFS
360 370 380 390 400
VVVLANIKML DIDPDRVNIN GGAVSLGHPI GMSGARIVGH MVHNLKSGQY
410 420
GLAGICNGGG GASSIVIQKF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC078651 mRNA Translation: AAH78651.1 |
RefSeqi | NP_001003746.1, NM_001003746.1 |
Genome annotation databases
Ensembli | ENSDART00000170182; ENSDARP00000133230; ENSDARG00000045888 |
GeneIDi | 445290 |
KEGGi | dre:445290 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC078651 mRNA Translation: AAH78651.1 |
RefSeqi | NP_001003746.1, NM_001003746.1 |
3D structure databases
SMRi | Q6AZA0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000067447 |
Proteomic databases
PaxDbi | Q6AZA0 |
PeptideAtlasi | Q6AZA0 |
PRIDEi | Q6AZA0 |
Genome annotation databases
Ensembli | ENSDART00000170182; ENSDARP00000133230; ENSDARG00000045888 |
GeneIDi | 445290 |
KEGGi | dre:445290 |
Organism-specific databases
CTDi | 38 |
ZFINi | ZDB-GENE-040808-68, acat1 |
Phylogenomic databases
eggNOGi | KOG1390, Eukaryota |
GeneTreei | ENSGT01020000230461 |
HOGENOMi | CLU_031026_0_1_1 |
InParanoidi | Q6AZA0 |
OrthoDBi | 797770at2759 |
PhylomeDBi | Q6AZA0 |
TreeFami | TF300650 |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
Reactomei | R-DRE-70895, Branched-chain amino acid catabolism R-DRE-77108, Utilization of Ketone Bodies R-DRE-77111, Synthesis of Ketone Bodies |
Miscellaneous databases
PROi | PR:Q6AZA0 |
Gene expression databases
Bgeei | ENSDARG00000045888, Expressed in heart and 33 other tissues |
ExpressionAtlasi | Q6AZA0, baseline and differential |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 2 hits |
InterProi | View protein in InterPro IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
PIRSFi | PIRSF000429, Ac-CoA_Ac_transf, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THIL_DANRE | |
Accessioni | Q6AZA0Primary (citable) accession number: Q6AZA0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 16, 2008 |
Last sequence update: | September 13, 2004 | |
Last modified: | April 7, 2021 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families