UniProtKB - Q6AYT7 (ABD12_RAT)
Lysophosphatidylserine lipase ABHD12
Abhd12
Functioni
Lysophosphatidylserine (LPS) lipase that mediates the hydrolysis of lysophosphatidylserine, a class of signaling lipids that regulates immunological and neurological processes (By similarity).
Represents a major lysophosphatidylserine lipase in the brain, thereby playing a key role in the central nervous system (By similarity).
Also able to hydrolyze oxidized phosphatidylserine; oxidized phosphatidylserine is produced in response to severe inflammatory stress and constitutes a proapoptotic 'eat me' signal. Also has monoacylglycerol (MAG) lipase activity: hydrolyzes 2-arachidonoylglycerol (2-AG), thereby acting as a regulator of endocannabinoid signaling pathways. Has a strong preference for very-long-chain lipid substrates; substrate specificity is likely due to improved catalysis and not improved substrate binding (By similarity).
By similarityCatalytic activityi
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-(9Z-octadecenoyl)-sn-glycerol + H+ + phosphocholineBy similarity
- 1-hexadecanoyl-sn-glycero-3-phospho-L-serine + H2O = H+ + hexadecanoate + sn-glycero-3-phospho-L-serineBy similarity
- 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H+By similarity
- 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H+By similarity
- Hydrolyzes glycerol monoesters of long-chain fatty acids.By similarity EC:3.1.1.23
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 246 | NucleophileBy similarity | 1 | |
Active sitei | 333 | Charge relay systemBy similarity | 1 | |
Active sitei | 372 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- acylglycerol lipase activity Source: UniProtKB
- lysophospholipase activity Source: UniProtKB
- palmitoyl-(protein) hydrolase activity Source: RGD
- phospholipase activity Source: UniProtKB
GO - Biological processi
- acylglycerol catabolic process Source: UniProtKB
- adult walking behavior Source: RGD
- glycerophospholipid catabolic process Source: RGD
- monoacylglycerol catabolic process Source: UniProtKB
- phosphatidylserine catabolic process Source: UniProtKB
- phospholipid catabolic process Source: UniProtKB
- protein depalmitoylation Source: RGD
- regulation of inflammatory response Source: RGD
- response to auditory stimulus Source: RGD
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-RNO-426048, Arachidonate production from DAG |
Protein family/group databases
ESTHERi | ratno-q6ayt7, ABHD12-PHARC |
Names & Taxonomyi
Protein namesi | Recommended name: Lysophosphatidylserine lipase ABHD12Curated (EC:3.1.-.-By similarity)Alternative name(s): |
Gene namesi | Name:Abhd12Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1562570, Abhd12 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
Plasma Membrane
- AMPA glutamate receptor complex Source: RGD
Other locations
- cytoplasm Source: RGD
- dendrite cytoplasm Source: RGD
- integral component of membrane Source: UniProtKB
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 74 | CytoplasmicBy similarityAdd BLAST | 74 | |
Transmembranei | 75 – 95 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 96 – 398 | ExtracellularBy similarityAdd BLAST | 303 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000375809 | 1 – 398 | Lysophosphatidylserine lipase ABHD12Add BLAST | 398 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 123 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
jPOSTi | Q6AYT7 |
PaxDbi | Q6AYT7 |
PRIDEi | Q6AYT7 |
PTM databases
GlyGeni | Q6AYT7, 1 site |
iPTMneti | Q6AYT7 |
PhosphoSitePlusi | Q6AYT7 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000036934, Expressed in cerebellum and 21 other tissues |
Genevisiblei | Q6AYT7, RN |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 24 | DisorderedSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1552, Eukaryota |
GeneTreei | ENSGT00940000160517 |
HOGENOMi | CLU_029375_1_0_1 |
InParanoidi | Q6AYT7 |
OMAi | YELHNCL |
OrthoDBi | 691954at2759 |
PhylomeDBi | Q6AYT7 |
TreeFami | TF315122 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR026605, ABHD12 IPR022742, Hydrolase_4 |
PANTHERi | PTHR12277:SF61, PTHR12277:SF61, 1 hit |
Pfami | View protein in Pfam PF12146, Hydrolase_4, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MRKRTEPVTL EHERCAASGS SSSGSAAAAL DADCSLKQNL RLAGKGTAEP
60 70 80 90 100
HSASDAGMKR ALGRRKSLWF RLRKILLCVL GFYIAIPFLV KLCPGIQAKL
110 120 130 140 150
IFLNFVRVPY FIDLKKPQDQ GLNHTCNYYL QPEDDVTIGV WHTIPSVWWK
160 170 180 190 200
NAQGKDQMWY EDALASNHPI ILYLHGNAGT RGGDHRVELY KVLSSLGYHV
210 220 230 240 250
VTFDYRGWGD SVGTPSERGM TYDALHVFDW IKARSGDNPV YIWGHSLGTG
260 270 280 290 300
VATNLVRRLC ERETPPDALI LESPFTNIRE EAKSHPFSVI YRYFPGFDWF
310 320 330 340 350
FLDPITSSGI KFANDENMKH ISCPLLILHA EDDPVVPFHL GRKLYNIAAP
360 370 380 390
SRSFRDFKVQ FIPFHSDLGY RHKYIYKSPE LPRILREFLG KSEPERQH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A8I6ACA9 | A0A8I6ACA9_RAT | Lysophosphatidylserine lipase ABHD1... | Abhd12 | 86 | Annotation score: | ||
A0A8I6ATI5 | A0A8I6ATI5_RAT | Lysophosphatidylserine lipase ABHD1... | Abhd12 | 365 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH474050 Genomic DNA Translation: EDL86136.1 BC078918 mRNA Translation: AAH78918.1 |
RefSeqi | NP_001019485.1, NM_001024314.1 |
Genome annotation databases
Ensembli | ENSRNOT00000010641; ENSRNOP00000010640; ENSRNOG00000036934 |
GeneIDi | 499913 |
KEGGi | rno:499913 |
UCSCi | RGD:1562570, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH474050 Genomic DNA Translation: EDL86136.1 BC078918 mRNA Translation: AAH78918.1 |
RefSeqi | NP_001019485.1, NM_001024314.1 |
3D structure databases
AlphaFoldDBi | Q6AYT7 |
SMRi | Q6AYT7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000010640 |
Chemistry databases
ChEMBLi | CHEMBL3708071 |
Protein family/group databases
ESTHERi | ratno-q6ayt7, ABHD12-PHARC |
PTM databases
GlyGeni | Q6AYT7, 1 site |
iPTMneti | Q6AYT7 |
PhosphoSitePlusi | Q6AYT7 |
Proteomic databases
jPOSTi | Q6AYT7 |
PaxDbi | Q6AYT7 |
PRIDEi | Q6AYT7 |
Genome annotation databases
Ensembli | ENSRNOT00000010641; ENSRNOP00000010640; ENSRNOG00000036934 |
GeneIDi | 499913 |
KEGGi | rno:499913 |
UCSCi | RGD:1562570, rat |
Organism-specific databases
CTDi | 26090 |
RGDi | 1562570, Abhd12 |
Phylogenomic databases
eggNOGi | KOG1552, Eukaryota |
GeneTreei | ENSGT00940000160517 |
HOGENOMi | CLU_029375_1_0_1 |
InParanoidi | Q6AYT7 |
OMAi | YELHNCL |
OrthoDBi | 691954at2759 |
PhylomeDBi | Q6AYT7 |
TreeFami | TF315122 |
Enzyme and pathway databases
Reactomei | R-RNO-426048, Arachidonate production from DAG |
Miscellaneous databases
PROi | PR:Q6AYT7 |
Gene expression databases
Bgeei | ENSRNOG00000036934, Expressed in cerebellum and 21 other tissues |
Genevisiblei | Q6AYT7, RN |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR026605, ABHD12 IPR022742, Hydrolase_4 |
PANTHERi | PTHR12277:SF61, PTHR12277:SF61, 1 hit |
Pfami | View protein in Pfam PF12146, Hydrolase_4, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ABD12_RAT | |
Accessioni | Q6AYT7Primary (citable) accession number: Q6AYT7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 26, 2009 |
Last sequence update: | September 13, 2004 | |
Last modified: | May 25, 2022 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families