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Protein

Transcription factor NF-E2 45 kDa subunit

Gene

Nfe2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor NF-E2 45 kDa subunit
Alternative name(s):
Leucine zipper protein NF-E2
Nuclear factor, erythroid-derived 2 45 kDa subunit
p45 NF-E2
Gene namesi
Name:Nfe2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1306888 Nfe2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003200791 – 373Transcription factor NF-E2 45 kDa subunitAdd BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157Phosphoserine; by MAPK8By similarity1
Modified residuei170Phosphoserine; by PKABy similarity1
Cross-linki368Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki368Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity

Post-translational modificationi

Phosphorylated on serine residues. In undifferentiated erythrocytes, phosphorylated by MAPK8 which then leads to ubiquitination and protein degradation.By similarity
Sumoylated. Sumoylation is required for translocation to nuclear bodies PODs, anchoring to the gene loci, and transactivation of the beta-globin gene.By similarity
Ubiquitinated mainly by 'Lys63'-linked ubiquitin. Polyubiquitination with 'Lys63'-linked ubiquitin by ITCH retains NFE2 in the cytoplasm preventing its transactivation activity. In undifferentiated erythrocyte, ubiquitinated after MAPK8-mediatd phosphorylation leading to protein degradation.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6AYT2
PRIDEiQ6AYT2

Expressioni

Gene expression databases

BgeeiENSRNOG00000036837 Expressed in 9 organ(s), highest expression level in spleen
GenevisibleiQ6AYT2 RN

Interactioni

Subunit structurei

Homodimer; can bind DNA as a homodimer (By similarity). Erythroid transcription activator nuclear factor erythroid-derived 2 (NF-E2), composed of a heterodimer of NFE2 and MAFK, possesses transactivation activity on beta-globin. Also forms high affinity heterodimer with MAFG; the interaction promotes erythropoiesis. Interacts (via the PXY motif 1) with ITCH (via the WW 1 domain); the interaction promotes 'Lys63'-linked ubiquitination of NFE2, translocates it to the cytoplasm and inhibits its transactivation activity. Interacts with KMT2D/MLL2; the interaction promotes transactivation of the beta-globin locus. Interacts with MAPK8 (phosphorylated form); the interaction leads to phosphorylation of NFE2 in undifferentiated cells (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052156

Structurei

3D structure databases

ProteinModelPortaliQ6AYT2
SMRiQ6AYT2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini266 – 329bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 206Transactivation domainBy similarityAdd BLAST206
Regioni1 – 83Required for interaction with MAPK8By similarityAdd BLAST83
Regioni268 – 287Basic motifPROSITE-ProRule annotationAdd BLAST20
Regioni291 – 298Leucine-zipperPROSITE-ProRule annotation8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 65PXY motif 15
Motifi79 – 83PXY motif 25

Domaini

The PXY motifs are required for binding WW domains. PXY1 is required to promote transactivation of beta-globin and for hyperacetylation of histone H3, but not for binding to the HS2 promoter site.By similarity

Sequence similaritiesi

Belongs to the bZIP family. CNC subfamily.Curated

Phylogenomic databases

eggNOGiENOG410ISV1 Eukaryota
ENOG410YEZR LUCA
GeneTreeiENSGT00920000148991
HOGENOMiHOG000234410
HOVERGENiHBG002901
InParanoidiQ6AYT2
KOiK09039
OMAiMELTWQE
OrthoDBiEOG091G02EB
PhylomeDBiQ6AYT2

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR004826 bZIP_Maf
IPR029853 NF-E2_p45
IPR008917 TF_DNA-bd_sf
PANTHERiPTHR24411:SF26 PTHR24411:SF26, 2 hits
PfamiView protein in Pfam
PF03131 bZIP_Maf, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit

Sequencei

Sequence statusi: Complete.

Q6AYT2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPPCPPQPNR NRLPQLPTGE LGEMELTWQE IMSITELQGL NVPSEPSFEP
60 70 80 90 100
QAPTPYPGPL PPPTYCPCSI HPDAGFTLPP PPYELPASTP HAPDLPYSYG
110 120 130 140 150
NIAIPVSKPL TLSGLLNEPL PDPLALLDIG LPVGQPKPQE DPESDSGLSL
160 170 180 190 200
NYSDAESLEL EGTEAGRRRS EYVDMYPVEY PYSLMPNSLA HPNYTLPPTE
210 220 230 240 250
TPLVLESSSG PVRAKPAVRG EAGSRDERRA LAMKIPFPTD KIVNLPVDDF
260 270 280 290 300
NELLAQYPLT ESQLALVRDI RRRGKNKVAA QNCRKRKLET IVQLERELER
310 320 330 340 350
LGSERERLLR ARGEADRTLE VMRQQLTELY HDIFQHLRDE SGNSYSPEEY
360 370
VLQQAADGAI FLVPRGTKME ATD
Length:373
Mass (Da):41,496
Last modified:September 13, 2004 - v1
Checksum:iD9140F9F23CAC23C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078925 mRNA Translation: AAH78925.1
RefSeqiNP_001012224.1, NM_001012224.1
XP_006242494.1, XM_006242432.3
XP_006242495.1, XM_006242433.3
XP_017450513.1, XM_017595024.1
UniGeneiRn.137607

Genome annotation databases

EnsembliENSRNOT00000055281; ENSRNOP00000052156; ENSRNOG00000036837
GeneIDi366998
KEGGirno:366998
UCSCiRGD:1306888 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078925 mRNA Translation: AAH78925.1
RefSeqiNP_001012224.1, NM_001012224.1
XP_006242494.1, XM_006242432.3
XP_006242495.1, XM_006242433.3
XP_017450513.1, XM_017595024.1
UniGeneiRn.137607

3D structure databases

ProteinModelPortaliQ6AYT2
SMRiQ6AYT2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052156

Proteomic databases

PaxDbiQ6AYT2
PRIDEiQ6AYT2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000055281; ENSRNOP00000052156; ENSRNOG00000036837
GeneIDi366998
KEGGirno:366998
UCSCiRGD:1306888 rat

Organism-specific databases

CTDi4778
RGDi1306888 Nfe2

Phylogenomic databases

eggNOGiENOG410ISV1 Eukaryota
ENOG410YEZR LUCA
GeneTreeiENSGT00920000148991
HOGENOMiHOG000234410
HOVERGENiHBG002901
InParanoidiQ6AYT2
KOiK09039
OMAiMELTWQE
OrthoDBiEOG091G02EB
PhylomeDBiQ6AYT2

Enzyme and pathway databases

ReactomeiR-RNO-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

PROiPR:Q6AYT2

Gene expression databases

BgeeiENSRNOG00000036837 Expressed in 9 organ(s), highest expression level in spleen
GenevisibleiQ6AYT2 RN

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR004826 bZIP_Maf
IPR029853 NF-E2_p45
IPR008917 TF_DNA-bd_sf
PANTHERiPTHR24411:SF26 PTHR24411:SF26, 2 hits
PfamiView protein in Pfam
PF03131 bZIP_Maf, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNFE2_RAT
AccessioniPrimary (citable) accession number: Q6AYT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: September 13, 2004
Last modified: September 12, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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