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Protein

7-methylguanosine phosphate-specific 5'-nucleotidase

Gene

Nt5c3b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates (By similarity).By similarity

Catalytic activityi

N7-methyl-GMP + H2O = N7-methyl-guanosine + phosphate.By similarity
CMP + H2O = cytidine + phosphate.By similarity
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei41NucleophileBy similarity1
Metal bindingi41MagnesiumBy similarity1
Active sitei43Proton donorBy similarity1
Metal bindingi43Magnesium; via carbonyl oxygenBy similarity1
Binding sitei88CMPBy similarity1
Binding sitei88N(7)-methyl-GMPBy similarity1
Binding sitei205SubstrateBy similarity1
Metal bindingi230MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-429958 mRNA decay by 3' to 5' exoribonuclease
SABIO-RKiQ6AYP7

Names & Taxonomyi

Protein namesi
Recommended name:
7-methylguanosine phosphate-specific 5'-nucleotidaseBy similarity (EC:3.1.3.91By similarity)
Short name:
7-methylguanosine nucleotidase
Alternative name(s):
Cytosolic 5'-nucleotidase 3B
Cytosolic 5'-nucleotidase III-like proteinBy similarity (EC:3.1.3.5By similarity)
Short name:
cN-III-like protein
N(7)-methylguanylate 5'-phosphatase
Gene namesi
Name:Nt5c3b
Synonyms:Nt5c3l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1359229 Nt5c3b

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003289501 – 3007-methylguanosine phosphate-specific 5'-nucleotidaseAdd BLAST300

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei256N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ6AYP7
PRIDEiQ6AYP7

Expressioni

Gene expression databases

BgeeiENSRNOG00000016475
ExpressionAtlasiQ6AYP7 baseline and differential
GenevisibleiQ6AYP7 RN

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022430

Structurei

3D structure databases

ProteinModelPortaliQ6AYP7
SMRiQ6AYP7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni156 – 157Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the pyrimidine 5'-nucleotidase family.Curated

Phylogenomic databases

eggNOGiKOG3128 Eukaryota
ENOG410ZQJ8 LUCA
GeneTreeiENSGT00390000012959
HOGENOMiHOG000244931
HOVERGENiHBG059750
InParanoidiQ6AYP7
KOiK01081
OMAiIISEECR
TreeFamiTF314663

Family and domain databases

CDDicd07504 HAD_5NT, 1 hit
Gene3Di3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006434 Pyrimidine_nucleotidase_eu
PfamiView protein in Pfam
PF05822 UMPH-1, 1 hit
SFLDiSFLDG01128 C1.4:_5'-Nucleotidase_Like, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01544 HAD-SF-IE, 1 hit

Sequencei

Sequence statusi: Complete.

Q6AYP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEEVSSLMK ATVLMRQPGR VQEIVSALRR GGGDRLQVIS DFDMTLSRFA
60 70 80 90 100
YNGQRCPSSH NILDNSKIIS EDCRKELTEL FHHYYPIEID PHRTIKEKLP
110 120 130 140 150
HMVQWWSKAH SLLCQQRIQK FQIAQVVGES TAMLREGYKM FFDTLYHNNI
160 170 180 190 200
PLFIFSAGIG DILEEIIRQM KVFHPNIHIV SNYMDFSEDG FLKGFKGQLI
210 220 230 240 250
HTYNKNSSVC ENSSYFQQLR NKTNIILLGD SIGDLTMADG VPGVQNILKI
260 270 280 290 300
GFLNDKVEER RERYMDSYDI VLEKDETLDV VNGLLQHILR QGDCVELQGS
Length:300
Mass (Da):34,536
Last modified:April 3, 2013 - v2
Checksum:i73AD8E974981E20A
GO

Sequence cautioni

The sequence AAH78963 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC078963 mRNA Translation: AAH78963.1 Different initiation.
RefSeqiNP_001007724.2, NM_001007723.2
XP_017452886.1, XM_017597397.1
UniGeneiRn.24114

Genome annotation databases

EnsembliENSRNOT00000022430; ENSRNOP00000022430; ENSRNOG00000016475
ENSRNOT00000080006; ENSRNOP00000069295; ENSRNOG00000016475
GeneIDi360629
KEGGirno:360629
UCSCiRGD:1359229 rat

Similar proteinsi

Entry informationi

Entry namei5NT3B_RAT
AccessioniPrimary (citable) accession number: Q6AYP7
Secondary accession number(s): F8WG43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 3, 2013
Last modified: February 28, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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