Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Exopolyphosphatase PRUNE1

Gene

Prune1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization.By similarity

Catalytic activityi

Diphosphate + H2O = 2 phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Activated by magnesium ions and inhibited by manganese ions. Inhibited by dipyridamole, moderately sensitive to IBMX and inhibited by vinpocetine (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28Manganese 1By similarity1
Metal bindingi30Manganese 2By similarity1
Metal bindingi106Manganese 1By similarity1
Metal bindingi106Manganese 2By similarity1
Metal bindingi179Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolyphosphatase PRUNE1 (EC:3.6.1.1)
Gene namesi
Name:Prune1
Synonyms:Prune
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1359521 Prune1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003379891 – 454Exopolyphosphatase PRUNE1Add BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei400PhosphoserineCombined sources1
Modified residuei411PhosphothreonineBy similarity1
Modified residuei415PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6AYG3
PRIDEiQ6AYG3

PTM databases

iPTMnetiQ6AYG3
PhosphoSitePlusiQ6AYG3

Expressioni

Gene expression databases

BgeeiENSRNOG00000021120
GenevisibleiQ6AYG3 RN

Interactioni

Subunit structurei

Homooligomer. Able to homodimerize via its C-terminal domain. Interacts with NME1. Interacts with GSK3; at focal adhesion complexes where paxillin and vinculin are colocalized. Interacts with alpha and beta tubulin.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028677

Structurei

3D structure databases

ProteinModelPortaliQ6AYG3
SMRiQ6AYG3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni394 – 421Essential for homodimerizationBy similarityAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi106 – 108DHH motifBy similarity3

Sequence similaritiesi

Belongs to the PPase class C family. Prune subfamily.Curated

Phylogenomic databases

eggNOGiKOG4129 Eukaryota
COG1227 LUCA
GeneTreeiENSGT00450000040262
HOGENOMiHOG000115724
HOVERGENiHBG058142
InParanoidiQ6AYG3
KOiK01514
OMAiIYMDLEA
OrthoDBiEOG091G0E88
PhylomeDBiQ6AYG3
TreeFamiTF323914

Family and domain databases

Gene3Di3.10.310.20, 1 hit
InterProiView protein in InterPro
IPR001667 DDH_dom
IPR038763 DHH_sf
IPR004097 DHHA2
IPR038222 DHHA2_dom_sf
PfamiView protein in Pfam
PF01368 DHH, 1 hit
PF02833 DHHA2, 1 hit
SMARTiView protein in SMART
SM01131 DHHA2, 1 hit
SUPFAMiSSF64182 SSF64182, 1 hit

Sequencei

Sequence statusi: Complete.

Q6AYG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDYLQDCRA ALQESRPLHV VLGNEACDLD SMVSALALAF YLTKTSEAED
60 70 80 90 100
IFIPVLNIKR SELPLRGDNV FFLQEVKIAE SALIFRDEID LLALHQAGQL
110 120 130 140 150
TLILVDHHML PKSDAALEEA VAEVLDHRPI EQKYCPPCHV SVELVGSCAT
160 170 180 190 200
LVAERILQGA PETLDRQTAA LLHGTIILDC VNMDAKIGKA TLKDNEYVEK
210 220 230 240 250
LEALFPDLPK RKDIFDSLQK AKFDVSGLTT EQMLRKDQKT IYRQGTKVAV
260 270 280 290 300
SAVYMDLEAF LQRSGLLSDL SAFCQDHSYD ALVAMAIFFN THNEPVRQLA
310 320 330 340 350
IFCPHEALRM TICGVLEQST SPALKLTPIP SISAHLQAYL QGNTQVSRKK
360 370 380 390 400
LLPVLQEALS AYLDSMRTPA GQLEAALGMS REQADKELDK ASNSLIAGLS
410 420 430 440 450
QDDEDPPLPP TPMNSLVDEC PLDQGLPKLS AEAVFEKCSQ ISLSQSTRAC

PSNK
Length:454
Mass (Da):49,998
Last modified:September 13, 2004 - v1
Checksum:i3DE36BDFB273C6A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079054 mRNA Translation: AAH79054.1
RefSeqiNP_001007698.1, NM_001007697.1
UniGeneiRn.214534

Genome annotation databases

EnsembliENSRNOT00000028677; ENSRNOP00000028677; ENSRNOG00000021120
GeneIDi310664
KEGGirno:310664
UCSCiRGD:1359521 rat

Similar proteinsi

Entry informationi

Entry nameiPRUN1_RAT
AccessioniPrimary (citable) accession number: Q6AYG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: September 13, 2004
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health