UniProtKB - Q6AYG3 (PRUN1_RAT)
Protein
Exopolyphosphatase PRUNE1
Gene
Prune1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization.By similarity
Catalytic activityi
- EC:3.6.1.1
Cofactori
Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
Activity regulationi
Activated by magnesium ions and inhibited by manganese ions. Inhibited by dipyridamole, moderately sensitive to IBMX and inhibited by vinpocetine (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 28 | Manganese 1By similarity | 1 | |
Metal bindingi | 30 | Manganese 2By similarity | 1 | |
Metal bindingi | 106 | Manganese 1By similarity | 1 | |
Metal bindingi | 106 | Manganese 2By similarity | 1 | |
Metal bindingi | 179 | Manganese 2By similarity | 1 |
GO - Molecular functioni
- exopolyphosphatase activity Source: GO_Central
- inorganic diphosphatase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- phosphatase activity Source: RGD
- tubulin binding Source: RGD
GO - Biological processi
- polyphosphate catabolic process Source: GO_Central
- regulation of microtubule polymerization Source: RGD
- regulation of neurogenesis Source: RGD
Keywordsi
Molecular function | Hydrolase |
Ligand | Manganese, Metal-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Prune1 Synonyms:Prune |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1359521, Prune1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm By similarity
- focal adhesion By similarity
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
- focal adhesion Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000337989 | 1 – 454 | Exopolyphosphatase PRUNE1Add BLAST | 454 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 400 | PhosphoserineCombined sources | 1 | |
Modified residuei | 411 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 415 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q6AYG3 |
PaxDbi | Q6AYG3 |
PRIDEi | Q6AYG3 |
PTM databases
iPTMneti | Q6AYG3 |
PhosphoSitePlusi | Q6AYG3 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000021120, Expressed in skeletal muscle tissue and 21 other tissues |
Genevisiblei | Q6AYG3, RN |
Interactioni
Subunit structurei
Homooligomer. Able to homodimerize via its C-terminal domain.
Interacts with NME1.
Interacts with GSK3; at focal adhesion complexes where paxillin and vinculin are colocalized.
Interacts with alpha and beta tubulin.
By similarityGO - Molecular functioni
- tubulin binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000028677 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 394 – 421 | Essential for homodimerizationBy similarityAdd BLAST | 28 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 106 – 108 | DHH motifBy similarity | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4129, Eukaryota |
GeneTreei | ENSGT00450000040262 |
HOGENOMi | CLU_019358_2_0_1 |
InParanoidi | Q6AYG3 |
OMAi | RHLLVWG |
OrthoDBi | 1545660at2759 |
PhylomeDBi | Q6AYG3 |
TreeFami | TF323914 |
Family and domain databases
Gene3Di | 3.10.310.20, 1 hit |
InterProi | View protein in InterPro IPR001667, DDH_dom IPR038763, DHH_sf IPR004097, DHHA2 IPR038222, DHHA2_dom_sf |
Pfami | View protein in Pfam PF01368, DHH, 1 hit PF02833, DHHA2, 1 hit |
SMARTi | View protein in SMART SM01131, DHHA2, 1 hit |
SUPFAMi | SSF64182, SSF64182, 1 hit |
i Sequence
Sequence statusi: Complete.
Q6AYG3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDYLQDCRA ALQESRPLHV VLGNEACDLD SMVSALALAF YLTKTSEAED
60 70 80 90 100
IFIPVLNIKR SELPLRGDNV FFLQEVKIAE SALIFRDEID LLALHQAGQL
110 120 130 140 150
TLILVDHHML PKSDAALEEA VAEVLDHRPI EQKYCPPCHV SVELVGSCAT
160 170 180 190 200
LVAERILQGA PETLDRQTAA LLHGTIILDC VNMDAKIGKA TLKDNEYVEK
210 220 230 240 250
LEALFPDLPK RKDIFDSLQK AKFDVSGLTT EQMLRKDQKT IYRQGTKVAV
260 270 280 290 300
SAVYMDLEAF LQRSGLLSDL SAFCQDHSYD ALVAMAIFFN THNEPVRQLA
310 320 330 340 350
IFCPHEALRM TICGVLEQST SPALKLTPIP SISAHLQAYL QGNTQVSRKK
360 370 380 390 400
LLPVLQEALS AYLDSMRTPA GQLEAALGMS REQADKELDK ASNSLIAGLS
410 420 430 440 450
QDDEDPPLPP TPMNSLVDEC PLDQGLPKLS AEAVFEKCSQ ISLSQSTRAC
PSNK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC079054 mRNA Translation: AAH79054.1 |
RefSeqi | NP_001007698.1, NM_001007697.1 |
Genome annotation databases
Ensembli | ENSRNOT00000028677; ENSRNOP00000028677; ENSRNOG00000021120 |
GeneIDi | 310664 |
KEGGi | rno:310664 |
UCSCi | RGD:1359521, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC079054 mRNA Translation: AAH79054.1 |
RefSeqi | NP_001007698.1, NM_001007697.1 |
3D structure databases
SMRi | Q6AYG3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000028677 |
PTM databases
iPTMneti | Q6AYG3 |
PhosphoSitePlusi | Q6AYG3 |
Proteomic databases
jPOSTi | Q6AYG3 |
PaxDbi | Q6AYG3 |
PRIDEi | Q6AYG3 |
Genome annotation databases
Ensembli | ENSRNOT00000028677; ENSRNOP00000028677; ENSRNOG00000021120 |
GeneIDi | 310664 |
KEGGi | rno:310664 |
UCSCi | RGD:1359521, rat |
Organism-specific databases
CTDi | 58497 |
RGDi | 1359521, Prune1 |
Phylogenomic databases
eggNOGi | KOG4129, Eukaryota |
GeneTreei | ENSGT00450000040262 |
HOGENOMi | CLU_019358_2_0_1 |
InParanoidi | Q6AYG3 |
OMAi | RHLLVWG |
OrthoDBi | 1545660at2759 |
PhylomeDBi | Q6AYG3 |
TreeFami | TF323914 |
Miscellaneous databases
PROi | PR:Q6AYG3 |
Gene expression databases
Bgeei | ENSRNOG00000021120, Expressed in skeletal muscle tissue and 21 other tissues |
Genevisiblei | Q6AYG3, RN |
Family and domain databases
Gene3Di | 3.10.310.20, 1 hit |
InterProi | View protein in InterPro IPR001667, DDH_dom IPR038763, DHH_sf IPR004097, DHHA2 IPR038222, DHHA2_dom_sf |
Pfami | View protein in Pfam PF01368, DHH, 1 hit PF02833, DHHA2, 1 hit |
SMARTi | View protein in SMART SM01131, DHHA2, 1 hit |
SUPFAMi | SSF64182, SSF64182, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRUN1_RAT | |
Accessioni | Q6AYG3Primary (citable) accession number: Q6AYG3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | September 13, 2004 | |
Last modified: | February 10, 2021 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families