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Protein

Target of rapamycin complex 2 subunit MAPKAP1

Gene

Mapkap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-421'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processStress response

Enzyme and pathway databases

ReactomeiR-RNO-1257604 PIP3 activates AKT signaling
R-RNO-389357 CD28 dependent PI3K/Akt signaling
R-RNO-5218920 VEGFR2 mediated vascular permeability
R-RNO-6804757 Regulation of TP53 Degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex 2 subunit MAPKAP1
Short name:
TORC2 subunit MAPKAP1
Alternative name(s):
Mitogen-activated protein kinase 2-associated protein 1
Stress-activated map kinase-interacting protein 1
Short name:
SAPK-interacting protein 1
Gene namesi
Name:Mapkap1
Synonyms:Mip1, Sin1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1305363 Mapkap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003280362 – 522Target of rapamycin complex 2 subunit MAPKAP1Add BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Modified residuei510PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6AYF1
PRIDEiQ6AYF1

PTM databases

iPTMnetiQ6AYF1
PhosphoSitePlusiQ6AYF1

Expressioni

Gene expression databases

BgeeiENSRNOG00000017583
GenevisibleiQ6AYF1 RN

Interactioni

Subunit structurei

Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2, NBN and SGK1. Interacts with CCDC28B (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023889

Structurei

3D structure databases

SMRiQ6AYF1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 267Interaction with NBNBy similarityAdd BLAST266
Regioni2 – 184Interaction with MAP3K2By similarityAdd BLAST183
Regioni468 – 522Interaction with ATF2By similarityAdd BLAST55

Sequence similaritiesi

Belongs to the SIN1 family.Curated

Phylogenomic databases

eggNOGiKOG3739 Eukaryota
ENOG410XR57 LUCA
GeneTreeiENSGT00390000000642
HOGENOMiHOG000044319
HOVERGENiHBG023148
InParanoidiQ6AYF1
KOiK20410
OMAiNAKFWPQ
OrthoDBiEOG091G0AVW
PhylomeDBiQ6AYF1
TreeFamiTF315174

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR031567 CRIM_dom
IPR011993 PH-like_dom_sf
IPR032679 Sin1_N
IPR031313 Sin1_PH_dom
PfamiView protein in Pfam
PF16978 CRIM, 1 hit
PF05422 SIN1, 1 hit
PF16979 SIN1_PH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6AYF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFLDNPTII LAHIRQSHVT SDDTGMCEMV LIDHDVDLEK THPPSVPGDS
60 70 80 90 100
GSEVQGSSGE TQGYIYAQSV DITSSWDFGI RRRSNTAQRL ERLRKERQNQ
110 120 130 140 150
IKCKNIQWKE RNSKQSAQEL KSLFEKKSLK EKPPSSGKQS ILSVRLEQCP
160 170 180 190 200
LQLNNPFNEY SKFDGKGHVG TTATKKIDVY LPLHSSQDRL LPMTVVTMAS
210 220 230 240 250
ARVQDLIGLI CWQYTSEGRE PKLNDNVSAY CLHIAEDDGE VDTDFPPLDS
260 270 280 290 300
NEPIHKFGFS TLALVEKYSS PGLTSKESLF VRINAAHGFS LIQVDNTKVT
310 320 330 340 350
MKEILLKALK RRKGSQKISG PQYRLEKQSE PNIGVDLEST LESQNAWEFC
360 370 380 390 400
LVRENSSRAD GVFEEDSQID IATVQDMLSS HHYKSFKVSM IHRLRFTTDV
410 420 430 440 450
QLGISGDKVE IDPVTNQKAS TKFWIKQKPI SIDCDLLCAC DLAEEKSPSH
460 470 480 490 500
AVFKLTYLSS HDYKHLYFES DAATVSEIVL KVNYILESRA STARADYFAQ
510 520
KQRKLNRRTS FSFQKEKKSG QQ
Length:522
Mass (Da):59,043
Last modified:September 13, 2004 - v1
Checksum:i7F96C228E60A8B88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079073 mRNA Translation: AAH79073.1
RefSeqiNP_001011964.1, NM_001011964.1
XP_006234011.1, XM_006233949.2
XP_006234012.1, XM_006233950.2
XP_017447140.1, XM_017591651.1
XP_017447141.1, XM_017591652.1
UniGeneiRn.105886

Genome annotation databases

EnsembliENSRNOT00000023889; ENSRNOP00000023889; ENSRNOG00000017583
GeneIDi296648
KEGGirno:296648

Similar proteinsi

Entry informationi

Entry nameiSIN1_RAT
AccessioniPrimary (citable) accession number: Q6AYF1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: September 13, 2004
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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