UniProtKB - Q6AYC2 (IRGM_RAT)
Protein
Immunity-related GTPase family M protein
Gene
Irgm
Organism
Rattus norvegicus (Rat)
Status
Functioni
Putative GTPase which is required for IFNG-mediated clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 86 – 93 | GTPBy similarity | 8 | |
Nucleotide bindingi | 111 – 115 | GTPBy similarity | 5 | |
Nucleotide bindingi | 193 – 195 | GTPBy similarity | 3 | |
Nucleotide bindingi | 234 – 236 | GTPBy similarity | 3 |
GO - Molecular functioni
- BH3 domain binding Source: RGD
- CARD domain binding Source: RGD
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB-KW
- protein kinase binding Source: RGD
- protein serine/threonine kinase activator activity Source: RGD
GO - Biological processi
- autophagosome assembly Source: RGD
- CAMKK-AMPK signaling cascade Source: RGD
- cellular response to interferon-beta Source: GO_Central
- cellular response to interferon-gamma Source: RGD
- cellular response to lipopolysaccharide Source: RGD
- cellular response to virus Source: RGD
- defense response Source: RGD
- defense response to bacterium Source: RGD
- defense response to Gram-negative bacterium Source: RGD
- innate immune response Source: RGD
- nucleotide-binding oligomerization domain containing 2 signaling pathway Source: RGD
- positive regulation by symbiont of host autophagy Source: RGD
- positive regulation of autophagosome maturation Source: RGD
- positive regulation of autophagy Source: RGD
- positive regulation of interferon-gamma-mediated signaling pathway Source: RGD
- positive regulation of peptidyl-serine phosphorylation Source: RGD
- positive regulation of peptidyl-threonine phosphorylation Source: RGD
- positive regulation of protein phosphorylation Source: RGD
- positive regulation of protein serine/threonine kinase activity Source: RGD
- protein destabilization Source: RGD
- protein lipidation involved in autophagosome assembly Source: RGD
- protein stabilization Source: RGD
- regulation of protein complex stability Source: RGD
- regulation of protein-containing complex assembly Source: RGD
- response to bacterium Source: RGD
Keywordsi
Molecular function | Hydrolase |
Biological process | Autophagy, Immunity, Innate immunity |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Irgm |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1305163, Irgm |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity
Plasma membrane
- Cell membrane By similarity
Other locations
- phagosome membrane By similarity
- autophagosome membrane By similarity
- phagocytic cup By similarity
Note: Behaves like an integral membrane protein. Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes. Preferentially associated with cis- and medial-Golgi.By similarity
Cytosol
- cytosol Source: RGD
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Endosome
- late endosome Source: RGD
Golgi apparatus
- Golgi apparatus Source: RGD
- Golgi membrane Source: RGD
Lysosome
- autolysosome Source: RGD
- lysosome Source: RGD
Mitochondrion
- mitochondrion Source: RGD
Plasma Membrane
- phagocytic cup Source: UniProtKB-SubCell
Other locations
- autophagosome Source: RGD
- autophagosome membrane Source: UniProtKB-SubCell
- cell projection Source: UniProtKB-KW
- phagocytic vesicle Source: RGD
- phagocytic vesicle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasmic vesicle, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000325751 | 1 – 411 | Immunity-related GTPase family M proteinAdd BLAST | 411 |
Proteomic databases
PaxDbi | Q6AYC2 |
PRIDEi | Q6AYC2 |
PTM databases
PhosphoSitePlusi | Q6AYC2 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000031138, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | Q6AYC2, RN |
Interactioni
GO - Molecular functioni
- BH3 domain binding Source: RGD
- CARD domain binding Source: RGD
- protein kinase binding Source: RGD
Protein-protein interaction databases
IntActi | Q6AYC2, 1 interactor |
STRINGi | 10116.ENSRNOP00000047579 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 77 – 253 | IRG-type GPROSITE-ProRule annotationAdd BLAST | 177 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 77 – 291 | Mediates targeting to cell membrane and phagosomesBy similarityAdd BLAST | 215 | |
Regioni | 352 – 376 | Mediates targeting to the GolgiBy similarityAdd BLAST | 25 |
Sequence similaritiesi
Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.PROSITE-ProRule annotationCurated
Phylogenomic databases
eggNOGi | ENOG502QS9R, Eukaryota |
GeneTreei | ENSGT00950000183007 |
HOGENOMi | CLU_015342_3_0_1 |
InParanoidi | Q6AYC2 |
OMAi | SCMNCNT |
OrthoDBi | 688334at2759 |
PhylomeDBi | Q6AYC2 |
TreeFami | TF331897 |
Family and domain databases
InterProi | View protein in InterPro IPR030385, G_IRG_dom IPR007743, Immunity-related_GTPase-like IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF05049, IIGP, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51716, G_IRG, 1 hit |
i Sequence
Sequence statusi: Complete.
Q6AYC2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKPSHKSCEA APLLPKMPET STHNAPLNLS FPSVPSYQIG CSSLPEISRS
60 70 80 90 100
TERALKEGKL PELVYGVKET VATLSQIPVS IFVTGDSGNG MSSFINALRI
110 120 130 140 150
IGHEEDASAP TGVVRTTQTR AEYSSSHFPN VVLWDLPGLG ATAQTVENYI
160 170 180 190 200
EEMKFSTCDL FIIVASEQFS SNHVKLAKII QSMGKRFYVI WTKLDRDLST
210 220 230 240 250
SVLSEVRLIQ NIQENIRENL QKEGVKEVPI FLVSNLDPLL HDFPELRNTL
260 270 280 290 300
QTDLSNIRCC EPLKTLYVIY EKIIGDKVAN WNQIIANGRL KSSLGVRDDD
310 320 330 340 350
DMGECLKRYR LIFGIDDESL QQIAHGMGTV VMEYKANIKS QDFHTLRRAD
360 370 380 390 400
WKLRLMTCTT VNALFCLFKF LPCLCHCFKR MRHKRMLLLV AKDTKNILKK
410
ILMDAVSPPQ I
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC079106 mRNA Translation: AAH79106.1 |
RefSeqi | NP_001012007.1, NM_001012007.1 XP_006246240.1, XM_006246178.2 |
Genome annotation databases
Ensembli | ENSRNOT00000045545; ENSRNOP00000047579; ENSRNOG00000031138 |
GeneIDi | 303090 |
KEGGi | rno:303090 |
UCSCi | RGD:1305163, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC079106 mRNA Translation: AAH79106.1 |
RefSeqi | NP_001012007.1, NM_001012007.1 XP_006246240.1, XM_006246178.2 |
3D structure databases
SMRi | Q6AYC2 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q6AYC2, 1 interactor |
STRINGi | 10116.ENSRNOP00000047579 |
PTM databases
PhosphoSitePlusi | Q6AYC2 |
Proteomic databases
PaxDbi | Q6AYC2 |
PRIDEi | Q6AYC2 |
Genome annotation databases
Ensembli | ENSRNOT00000045545; ENSRNOP00000047579; ENSRNOG00000031138 |
GeneIDi | 303090 |
KEGGi | rno:303090 |
UCSCi | RGD:1305163, rat |
Organism-specific databases
CTDi | 345611 |
RGDi | 1305163, Irgm |
Phylogenomic databases
eggNOGi | ENOG502QS9R, Eukaryota |
GeneTreei | ENSGT00950000183007 |
HOGENOMi | CLU_015342_3_0_1 |
InParanoidi | Q6AYC2 |
OMAi | SCMNCNT |
OrthoDBi | 688334at2759 |
PhylomeDBi | Q6AYC2 |
TreeFami | TF331897 |
Miscellaneous databases
PROi | PR:Q6AYC2 |
Gene expression databases
Bgeei | ENSRNOG00000031138, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | Q6AYC2, RN |
Family and domain databases
InterProi | View protein in InterPro IPR030385, G_IRG_dom IPR007743, Immunity-related_GTPase-like IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF05049, IIGP, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51716, G_IRG, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IRGM_RAT | |
Accessioni | Q6AYC2Primary (citable) accession number: Q6AYC2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | September 13, 2004 | |
Last modified: | April 7, 2021 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families