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Protein

NADH-cytochrome b5 reductase 2

Gene

Cyb5r2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1) (By similarity).By similarity

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FADBy similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 137FADBy similarityAdd BLAST31
Nucleotide bindingi146 – 181FADBy similarityAdd BLAST36

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

ReactomeiR-RNO-1237044 Erythrocytes take up carbon dioxide and release oxygen

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-cytochrome b5 reductase 2 (EC:1.6.2.2)
Short name:
b5R.2
Gene namesi
Name:Cyb5r2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1308421 Cyb5r2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002875501 – 276NADH-cytochrome b5 reductase 2Add BLAST276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17N6-acetyllysineBy similarity1
Modified residuei18PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6AY12
PRIDEiQ6AY12

PTM databases

PhosphoSitePlusiQ6AY12

Expressioni

Gene expression databases

BgeeiENSRNOG00000019751
ExpressionAtlasiQ6AY12 baseline and differential
GenevisibleiQ6AY12 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026744

Structurei

3D structure databases

ProteinModelPortaliQ6AY12
SMRiQ6AY12
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 127FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0534 Eukaryota
COG0543 LUCA
GeneTreeiENSGT00390000008881
HOGENOMiHOG000175005
HOVERGENiHBG052580
InParanoidiQ6AY12
KOiK00326
PhylomeDBiQ6AY12

Family and domain databases

Gene3Di3.40.50.80, 1 hit
InterProiView protein in InterPro
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR039261 FNR_nucleotide_bd
IPR001834 NADH-Cyt_B5_reductase
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00371 FPNCR
SUPFAMiSSF52343 SSF52343, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

Q6AY12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVKKKDLIT LQDPEAKYPL PLIEKEQINH NTRRFRFGLP SPDHVLGLPV
60 70 80 90 100
GNYVHLLAQI NNELVIRAYT PVSSDDDQGF VDLIIKIYFK NVHPKYPEGG
110 120 130 140 150
KMTQYLENMK IGDTILFRGP TGRLFYNEPG TLLIKTDKTS EPEKKLVHHL
160 170 180 190 200
GMIAGGTGIT PMLQLIRHIT KDTSDGTRMS LLFANQTEED ILLRKELEEV
210 220 230 240 250
ATTHQNQFSL WYTLDRPPSG WEYSSGFITA DMIKEHLPPP GEATLILVCG
260 270
PPPLIQEAAH PSLEQLGYTK DMIFTY
Length:276
Mass (Da):31,239
Last modified:September 13, 2004 - v1
Checksum:i2B44235766324E96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC079235 mRNA Translation: AAH79235.1
RefSeqiNP_001014266.1, NM_001014244.1
UniGeneiRn.16198

Genome annotation databases

EnsembliENSRNOT00000026744; ENSRNOP00000026744; ENSRNOG00000019751
GeneIDi365345
KEGGirno:365345
UCSCiRGD:1308421 rat

Similar proteinsi

Entry informationi

Entry nameiNB5R2_RAT
AccessioniPrimary (citable) accession number: Q6AY12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: September 13, 2004
Last modified: July 18, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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