UniProtKB - Q6AXF6 (SIDT1_MOUSE)
Protein
SID1 transmembrane family member 1
Gene
Sidt1
Organism
Mus musculus (Mouse)
Status
Functioni
In vitro binds long double-stranded RNA (dsRNA) (500 and 700 base pairs), but not dsRNA shorter than 300 bp (PubMed:26067272). Not involved in RNA autophagy, a process in which RNA is directly imported into lysosomes in an ATP-dependent manner, and degraded (PubMed:27046251).2 Publications
GO - Molecular functioni
- cholesterol binding Source: MGI
- double-stranded RNA binding Source: WormBase
- RNA transmembrane transporter activity Source: GO_Central
GO - Biological processi
- RNA transport Source: GO_Central
Keywordsi
Molecular function | RNA-binding |
Names & Taxonomyi
Protein namesi | Recommended name: SID1 transmembrane family member 1 |
Gene namesi | Name:Sidt1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2443155, Sidt1 |
Subcellular locationi
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
Lysosome
- lysosome Source: GO_Central
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 309 | ExtracellularSequence analysisAdd BLAST | 290 | |
Transmembranei | 310 – 330 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 331 – 442 | CytoplasmicSequence analysisAdd BLAST | 112 | |
Transmembranei | 443 – 463 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 464 – 494 | ExtracellularSequence analysisAdd BLAST | 31 | |
Transmembranei | 495 – 515 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 516 – 541 | CytoplasmicSequence analysisAdd BLAST | 26 | |
Transmembranei | 542 – 562 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 563 – 572 | ExtracellularSequence analysis | 10 | |
Transmembranei | 573 – 590 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 591 – 600 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 601 – 621 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 622 – 626 | ExtracellularSequence analysis | 5 | |
Transmembranei | 627 – 647 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 648 – 683 | CytoplasmicSequence analysisAdd BLAST | 36 | |
Transmembranei | 684 – 704 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 705 – 710 | ExtracellularSequence analysis | 6 | |
Transmembranei | 711 – 731 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 732 – 741 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 742 – 762 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 763 – 791 | ExtracellularSequence analysisAdd BLAST | 29 | |
Transmembranei | 792 – 812 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 813 – 827 | CytoplasmicSequence analysisAdd BLAST | 15 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 169 | F → T: Decreased affinity for 700-bp RNA. 1 Publication | 1 | |
Mutagenesisi | 186 | P → L: Decreased affinity for 700-bp RNA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000032576 | 20 – 827 | SID1 transmembrane family member 1Add BLAST | 808 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 67 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 83 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 136 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 282 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 471 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 567 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 764 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q6AXF6 |
PRIDEi | Q6AXF6 |
PTM databases
GlyConnecti | 2711, 5 N-Linked glycans (2 sites) |
GlyGeni | Q6AXF6, 7 sites |
iPTMneti | Q6AXF6 |
PhosphoSitePlusi | Q6AXF6 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000022696, Expressed in primary visual cortex and 125 other tissues |
ExpressionAtlasi | Q6AXF6, baseline and differential |
Genevisiblei | Q6AXF6, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038433 |
Miscellaneous databases
RNActi | Q6AXF6, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the SID1 family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QUXZ, Eukaryota |
GeneTreei | ENSGT00390000010091 |
HOGENOMi | CLU_059625_0_0_1 |
InParanoidi | Q6AXF6 |
OMAi | QPGQVNA |
OrthoDBi | 139174at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR025958, SID1_TM_fam |
PANTHERi | PTHR12185, PTHR12185, 1 hit |
Pfami | View protein in Pfam PF13965, SID-1_RNA_chan, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q6AXF6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLDCLRLALL CALPWLLRAA VPGHQEEPLA KSAELRRDPR DPARGADFDR
60 70 80 90 100
VYSGVVSLST ENIYSFNHTS HPGQVTAVRV HVNSSSDNLD YPVLVVVRQQ
110 120 130 140 150
KEVLSWQVPL LFQGLYQRSY NYQEVSRTLC PSKATNETGP LEQLIFVDVA
160 170 180 190 200
SMAPHGAHYK LLVTKIKHFQ LPTNVAFYFT ASPSQPQYFL YKFPEDVDSV
210 220 230 240 250
IIKVVSEKAY PCSVVSVQNI MCPVYDLDHN VEFNGVYQSM TKKAAITLQK
260 270 280 290 300
KDFPDEQFFV VFVIKPEDYA CGGSFSIQEN ENQTWNLQRS KNLKVTIVPS
310 320 330 340 350
IKESVYVKSS LFSIFVFLSF YLGCLLVVLV HHVRFQRKSI DGSFGSSDGS
360 370 380 390 400
GNMAVSHPIT ASTPEGSNYG AIDESSSSPG RQMSSSDGGQ PCHSDTDSSV
410 420 430 440 450
EESDFDTMPD IESDKNVIRT KMFLYLSDLS RKDRRIVSKK YKIYFWNIIT
460 470 480 490 500
IAVFYALPVM QLVITYQTVV NVTGNQDICY YNFLCAHPLG VLSAFNNILS
510 520 530 540 550
NLGHVLLGFL FLLIVLRRDL LHRRALEAKD IFAMEYGIPK HFGLFYAMGI
560 570 580 590 600
ALMMEGVLSA CYHVCPNYSN FQFDTSFMYM IAGLCMLKLY QTRHPDINAS
610 620 630 640 650
AYSAYASFAV VITLTVLGVV FGKNDVWFWI IFSAIHILSS LALSTQIYYM
660 670 680 690 700
GRFKIDLGIF RRAAMVFYTD CIQQCSRPLY MDRMVLLIVG NLVNWSFAFF
710 720 730 740 750
GLIYRPRDFA SYMLGIFICN LLLYLAFYII MKLRSSEKVL PLPVFCIAAT
760 770 780 790 800
AVVWAAALYF FFQNLSSWEG TPAESREKNR ECVLLDFFDD HDIWHFLSAT
810 820
ALFFSFLVLL TLDDDLDVVR RDQIPVF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q247 | E9Q247_MOUSE | SID1 transmembrane family member 1 | Sidt1 | 832 | Annotation score: | ||
F6YLH2 | F6YLH2_MOUSE | SID1 transmembrane family member 1 | Sidt1 | 62 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013522 | 1 – 382 | Missing in isoform 2. 1 PublicationAdd BLAST | 382 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC025888 mRNA Translation: AAH25888.1 BC079584 mRNA Translation: AAH79584.1 |
CCDSi | CCDS28185.1 [Q6AXF6-1] |
RefSeqi | NP_001152891.1, NM_001159419.1 NP_932151.2, NM_198034.3 [Q6AXF6-1] |
Genome annotation databases
Ensembli | ENSMUST00000136381; ENSMUSP00000115372; ENSMUSG00000022696 [Q6AXF6-1] |
GeneIDi | 320007 |
KEGGi | mmu:320007 |
UCSCi | uc007zhd.1, mouse [Q6AXF6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC025888 mRNA Translation: AAH25888.1 BC079584 mRNA Translation: AAH79584.1 |
CCDSi | CCDS28185.1 [Q6AXF6-1] |
RefSeqi | NP_001152891.1, NM_001159419.1 NP_932151.2, NM_198034.3 [Q6AXF6-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038433 |
PTM databases
GlyConnecti | 2711, 5 N-Linked glycans (2 sites) |
GlyGeni | Q6AXF6, 7 sites |
iPTMneti | Q6AXF6 |
PhosphoSitePlusi | Q6AXF6 |
Proteomic databases
PaxDbi | Q6AXF6 |
PRIDEi | Q6AXF6 |
Protocols and materials databases
Antibodypediai | 32584, 151 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000136381; ENSMUSP00000115372; ENSMUSG00000022696 [Q6AXF6-1] |
GeneIDi | 320007 |
KEGGi | mmu:320007 |
UCSCi | uc007zhd.1, mouse [Q6AXF6-1] |
Organism-specific databases
CTDi | 54847 |
MGIi | MGI:2443155, Sidt1 |
Phylogenomic databases
eggNOGi | ENOG502QUXZ, Eukaryota |
GeneTreei | ENSGT00390000010091 |
HOGENOMi | CLU_059625_0_0_1 |
InParanoidi | Q6AXF6 |
OMAi | QPGQVNA |
OrthoDBi | 139174at2759 |
Miscellaneous databases
BioGRID-ORCSi | 320007, 0 hits in 17 CRISPR screens |
ChiTaRSi | Sidt1, mouse |
PROi | PR:Q6AXF6 |
RNActi | Q6AXF6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022696, Expressed in primary visual cortex and 125 other tissues |
ExpressionAtlasi | Q6AXF6, baseline and differential |
Genevisiblei | Q6AXF6, MM |
Family and domain databases
InterProi | View protein in InterPro IPR025958, SID1_TM_fam |
PANTHERi | PTHR12185, PTHR12185, 1 hit |
Pfami | View protein in Pfam PF13965, SID-1_RNA_chan, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SIDT1_MOUSE | |
Accessioni | Q6AXF6Primary (citable) accession number: Q6AXF6 Secondary accession number(s): Q8R397 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | September 13, 2004 | |
Last modified: | December 2, 2020 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families