UniProtKB - Q6A152 (CP4X1_MOUSE)
Cytochrome P450 4X1
Cyp4x1
Functioni
A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
By similarityCatalytic activityi
- N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine + O2 + reduced [NADPH—hemoprotein reductase] = H+ + H2O + N-(14,15-epoxy-5Z,8Z,11Z-eicosatrienoyl)-ethanolamine + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 452 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- anandamide 14,15 epoxidase activity Source: UniProtKB
- arachidonic acid 14,15-epoxygenase activity Source: MGI
- fatty acid in-chain hydroxylase activity Source: MGI
- heme binding Source: InterPro
- iron ion binding Source: InterPro
GO - Biological processi
- lipid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Lipid metabolism |
Ligand | Heme, Iron, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 4X11 Publication (EC:1.14.14.-By similarity)Alternative name(s): CYPIVX1 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1932403, Cyp4x1 |
VEuPathDBi | HostDB:ENSMUSG00000047155 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein Sequence analysis
- Microsome membrane 1 Publication; Single-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 14 – 34 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000421683 | 1 – 507 | Cytochrome P450 4X1Add BLAST | 507 |
Proteomic databases
EPDi | Q6A152 |
jPOSTi | Q6A152 |
MaxQBi | Q6A152 |
PaxDbi | Q6A152 |
PRIDEi | Q6A152 |
ProteomicsDBi | 285283 |
PTM databases
iPTMneti | Q6A152 |
PhosphoSitePlusi | Q6A152 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000047155, Expressed in substantia nigra and 126 other tissues |
ExpressionAtlasi | Q6A152, baseline and differential |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000059545 |
Miscellaneous databases
RNActi | Q6A152, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0157, Eukaryota |
GeneTreei | ENSGT00940000160927 |
InParanoidi | Q6A152 |
OMAi | SDQRHPY |
OrthoDBi | 1247045at2759 |
PhylomeDBi | Q6A152 |
TreeFami | TF105088 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MEASWLETRW ARPLHLALVF CLALVLMQAM KLYLRRQRLL RDLSPFPGPP
60 70 80 90 100
AHWLLGHQKF LQEDNMETLD EIVKKHPCAF PCWVGPFQAF FYIYDPDYAK
110 120 130 140 150
IFLSRTDPKM QYLHQLLTPC IGRGLLNLDG PRWFQHRCLL TPAFHQDILK
160 170 180 190 200
PCVDTMAHSV KVMLDKWEKM WTTQETTIEV FEHINLMTLD IIMKCAFGQE
210 220 230 240 250
TNCQINGTYE SYVKATFELG EIISSRLYNF WHHHDIIFKL SPKGHCFQEL
260 270 280 290 300
GKVIHQYTEK IIQDRKKILK NQVKQDDTQT SQIFLDIVLS AQAEDERAFS
310 320 330 340 350
DADLRAEVNT FMWAGHDASA ASISWLLYCL ALNPEHQDRC RTEIRSILGD
360 370 380 390 400
GSSITWEQLD EMSYTTMCIK ETLRLIPPVP SISRELSKPL TLPDGHSLPA
410 420 430 440 450
GMTVVLSIWG LHHNPAVWND PKVFDPLRFT KENSDQRHPC AFLPFSSGPR
460 470 480 490 500
NCIGQQFAML ELKVAIALIL LHFQVAPDLT RPPAFSSHTV LRPKHGIYLH
LKKLLEC
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2A9Y7 | A2A9Y7_MOUSE | Cytochrome P450 4X1 | Cyp4x1 | 481 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 9 | R → G in BAC30028 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 110 | M → T in AAI13126 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 188 | T → A in AAI13126 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 327 | L → P in AAI13126 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ786769 mRNA Translation: CAH10751.1 AL645473 Genomic DNA No translation available. BC113125 mRNA Translation: AAI13126.1 AK038526 mRNA Translation: BAC30028.1 |
CCDSi | CCDS18488.1 |
RefSeqi | NP_001003947.1, NM_001003947.1 |
Genome annotation databases
Ensembli | ENSMUST00000051400; ENSMUSP00000059545; ENSMUSG00000047155 |
GeneIDi | 81906 |
KEGGi | mmu:81906 |
UCSCi | uc008uep.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ786769 mRNA Translation: CAH10751.1 AL645473 Genomic DNA No translation available. BC113125 mRNA Translation: AAI13126.1 AK038526 mRNA Translation: BAC30028.1 |
CCDSi | CCDS18488.1 |
RefSeqi | NP_001003947.1, NM_001003947.1 |
3D structure databases
AlphaFoldDBi | Q6A152 |
SMRi | Q6A152 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000059545 |
PTM databases
iPTMneti | Q6A152 |
PhosphoSitePlusi | Q6A152 |
Proteomic databases
EPDi | Q6A152 |
jPOSTi | Q6A152 |
MaxQBi | Q6A152 |
PaxDbi | Q6A152 |
PRIDEi | Q6A152 |
ProteomicsDBi | 285283 |
Protocols and materials databases
Antibodypediai | 32842, 215 antibodies from 24 providers |
DNASUi | 81906 |
Genome annotation databases
Ensembli | ENSMUST00000051400; ENSMUSP00000059545; ENSMUSG00000047155 |
GeneIDi | 81906 |
KEGGi | mmu:81906 |
UCSCi | uc008uep.1, mouse |
Organism-specific databases
CTDi | 260293 |
MGIi | MGI:1932403, Cyp4x1 |
VEuPathDBi | HostDB:ENSMUSG00000047155 |
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
GeneTreei | ENSGT00940000160927 |
InParanoidi | Q6A152 |
OMAi | SDQRHPY |
OrthoDBi | 1247045at2759 |
PhylomeDBi | Q6A152 |
TreeFami | TF105088 |
Miscellaneous databases
BioGRID-ORCSi | 81906, 1 hit in 72 CRISPR screens |
PROi | PR:Q6A152 |
RNActi | Q6A152, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000047155, Expressed in substantia nigra and 126 other tissues |
ExpressionAtlasi | Q6A152, baseline and differential |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP4X1_MOUSE | |
Accessioni | Q6A152Primary (citable) accession number: Q6A152 Secondary accession number(s): Q2HJC6, Q8BYS0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 6, 2013 |
Last sequence update: | September 13, 2004 | |
Last modified: | May 25, 2022 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families