UniProtKB - Q6A0D4 (RFTN1_MOUSE)
Protein
Raftlin
Gene
Rftn1
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in protein trafficking via association with clathrin and AP2 complex (By similarity). Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages, and internalization of poly(I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells; resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production (PubMed:27022195). Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion (PubMed:19414744). May regulate B-cell antigen receptor-mediated signaling (By similarity). May play a pivotal role in the formation and/or maintenance of lipid rafts (By similarity).By similarity2 Publications
GO - Molecular functioni
- double-stranded RNA binding Source: MGI
GO - Biological processi
- B cell receptor signaling pathway Source: MGI
- dsRNA transport Source: MGI
- interleukin-17 production Source: UniProtKB
- membrane raft assembly Source: MGI
- positive regulation of growth rate Source: MGI
- protein localization to membrane raft Source: UniProtKB
- protein transport into membrane raft Source: UniProtKB
- response to exogenous dsRNA Source: MGI
- T cell antigen processing and presentation Source: UniProtKB
- T cell receptor signaling pathway Source: UniProtKB
- toll-like receptor 3 signaling pathway Source: MGI
Names & Taxonomyi
Protein namesi | Recommended name: RaftlinAlternative name(s): Raft-linking protein |
Gene namesi | Name:Rftn1 Synonyms:Kiaa0084 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1923688, Rftn1 |
Subcellular locationi
Endosome
- Endosome By similarity
- Early endosome By similarity
Plasma membrane
- Cell membrane 1 Publication; Lipid-anchor By similarity
Other locations
- Cytoplasm By similarity
- Membrane raft By similarity
Note: Translocates from cytoplasm to cell membrane where it colocalizes with poly (I:C) and then moves to endosomes where it colocalizes with TLR3. Translocates from cytoplasm to cell membrane where it colocalizes with TLR4 and then together with TLR4 moves to endosomes, upon lipopolysaccharide stimulation (PubMed:27022195).By similarity
Endosome
- early endosome Source: UniProtKB-SubCell
- endosome Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
- membrane raft Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Endosome, MembranePathology & Biotechi
Disruption phenotypei
Mutant mice have smaller spleen, fewer splenocytes, reduced IgM production and secrete less IFNG, during antigen re-stimulation (PubMed:19414744). Double RFTN1 and RFTN2 mutant mice show no visible phenotype under pathogen-free conditions but show greatly reduced IFNB1 production in splenic dendritic cells following poly(I:C) or LPS stimulation (PubMed:27022195).2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000251954 | 2 – 554 | RaftlinAdd BLAST | 553 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Lipidationi | 3 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 183 | PhosphoserineCombined sources | 1 | |
Modified residuei | 199 | PhosphoserineBy similarity | 1 | |
Modified residuei | 507 | PhosphoserineCombined sources | 1 | |
Modified residuei | 530 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Lipoprotein, Myristate, Palmitate, PhosphoproteinProteomic databases
EPDi | Q6A0D4 |
jPOSTi | Q6A0D4 |
MaxQBi | Q6A0D4 |
PaxDbi | Q6A0D4 |
PeptideAtlasi | Q6A0D4 |
PRIDEi | Q6A0D4 |
PTM databases
iPTMneti | Q6A0D4 |
PhosphoSitePlusi | Q6A0D4 |
SwissPalmi | Q6A0D4 |
Expressioni
Tissue specificityi
Expressed in T-cells, B-cells, thymus and spleen (at protein level) (PubMed:19414744, PubMed:12805216). Expressed in dendritic cells, macrophages, heart, lung and small intestine (PubMed:19414744).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000039316, Expressed in mesenteric lymph node and 250 other tissues |
ExpressionAtlasi | Q6A0D4, baseline and differential |
Genevisiblei | Q6A0D4, MM |
Interactioni
Subunit structurei
Interacts with TLR4; the interaction occurs in response to lipopolysaccharide stimulation.
Interacts with CLTC; the interaction occurs in response to pathogens.
Interacts with AP2A1 and AP2B1.
By similarityProtein-protein interaction databases
BioGRIDi | 218128, 1 interactor |
STRINGi | 10090.ENSMUSP00000046524 |
Miscellaneous databases
RNActi | Q6A0D4, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the raftlin family.Curated
Phylogenomic databases
eggNOGi | ENOG502QVP2, Eukaryota |
GeneTreei | ENSGT00530000063609 |
InParanoidi | Q6A0D4 |
OMAi | LYNHTEE |
OrthoDBi | 434232at2759 |
PhylomeDBi | Q6A0D4 |
TreeFami | TF333285 |
Family and domain databases
InterProi | View protein in InterPro IPR028169, Raftlin |
PANTHERi | PTHR17601, PTHR17601, 1 hit |
Pfami | View protein in Pfam PF15250, Raftlin, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q6A0D4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGCSLNKLEK REEKRPGNIY STLKRPQVET KVDVTYEYCF LEFTTLTAAE
60 70 80 90 100
LPRSSATRLA SLRDLPDQLL ELYQQGFSLA ALHPFVQPTR RQEKILLEHI
110 120 130 140 150
FRAILVKKTN RSQKAELHDE GYTLELDYCS SLEHLADQKL IPEFIKKVQE
160 170 180 190 200
AASQGLKFVS VVPQYQPSVS SAGSRRLKPV ANSVEDARDV KCTLGDRSSL
210 220 230 240 250
ENDTPKAAET DAATAGVNRR PETKPGSTGD VPSAQQPGIP SPSAENEAGE
260 270 280 290 300
FPLRGLQPAL DRSEGDPSNG PEELPSRKME IFAFFNRPKS QQKCRQYYPV
310 320 330 340 350
TIPLQVSKNG QTVSSLDASW LEHMSDHFRK GGVLVNAVFQ LGMANDSFYG
360 370 380 390 400
LTDGVFIFEA VSTEDNRTTQ GYDAIVVEQW TVLEGTEVQT DYMPLLNSLA
410 420 430 440 450
AYGWQLTCVL PTPILKTTRE GNVSTKQIVF LQRPCLPQKT KKRESKFQWR
460 470 480 490 500
FSRNEIHGRQ TRKSKGKLSA SNKQQAEENE KNLEDQFSKA GDVGNCVLGA
510 520 530 540 550
PQWGRASEVR EQRQGSAAVQ NGPAGHNRDS VALRHSNPRA EAELAAGPTP
TEAN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3Z731 | D3Z731_MOUSE | Raftlin | Rftn1 | 276 | Annotation score: |
Sequence cautioni
The sequence BAD32162 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE31871 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 236 | Q → R in BAE30971 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 236 | Q → R in BAE31871 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 472 | N → D in BAD32162 (PubMed:15368895).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK172884 mRNA Translation: BAD32162.1 Different initiation. AK152130 mRNA Translation: BAE30971.1 AK153288 mRNA Translation: BAE31871.1 Different initiation. AK155870 mRNA Translation: BAE33473.1 BC052074 mRNA Translation: AAH52074.1 |
CCDSi | CCDS28873.1 |
RefSeqi | NP_852062.1, NM_181397.2 |
Genome annotation databases
Ensembli | ENSMUST00000044503; ENSMUSP00000046524; ENSMUSG00000039316 |
GeneIDi | 76438 |
KEGGi | mmu:76438 |
UCSCi | uc008cyr.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK172884 mRNA Translation: BAD32162.1 Different initiation. AK152130 mRNA Translation: BAE30971.1 AK153288 mRNA Translation: BAE31871.1 Different initiation. AK155870 mRNA Translation: BAE33473.1 BC052074 mRNA Translation: AAH52074.1 |
CCDSi | CCDS28873.1 |
RefSeqi | NP_852062.1, NM_181397.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 218128, 1 interactor |
STRINGi | 10090.ENSMUSP00000046524 |
PTM databases
iPTMneti | Q6A0D4 |
PhosphoSitePlusi | Q6A0D4 |
SwissPalmi | Q6A0D4 |
Proteomic databases
EPDi | Q6A0D4 |
jPOSTi | Q6A0D4 |
MaxQBi | Q6A0D4 |
PaxDbi | Q6A0D4 |
PeptideAtlasi | Q6A0D4 |
PRIDEi | Q6A0D4 |
Protocols and materials databases
Antibodypediai | 53317, 110 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000044503; ENSMUSP00000046524; ENSMUSG00000039316 |
GeneIDi | 76438 |
KEGGi | mmu:76438 |
UCSCi | uc008cyr.1, mouse |
Organism-specific databases
CTDi | 23180 |
MGIi | MGI:1923688, Rftn1 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | ENOG502QVP2, Eukaryota |
GeneTreei | ENSGT00530000063609 |
InParanoidi | Q6A0D4 |
OMAi | LYNHTEE |
OrthoDBi | 434232at2759 |
PhylomeDBi | Q6A0D4 |
TreeFami | TF333285 |
Miscellaneous databases
BioGRID-ORCSi | 76438, 2 hits in 17 CRISPR screens |
ChiTaRSi | Rftn1, mouse |
PROi | PR:Q6A0D4 |
RNActi | Q6A0D4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039316, Expressed in mesenteric lymph node and 250 other tissues |
ExpressionAtlasi | Q6A0D4, baseline and differential |
Genevisiblei | Q6A0D4, MM |
Family and domain databases
InterProi | View protein in InterPro IPR028169, Raftlin |
PANTHERi | PTHR17601, PTHR17601, 1 hit |
Pfami | View protein in Pfam PF15250, Raftlin, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RFTN1_MOUSE | |
Accessioni | Q6A0D4Primary (citable) accession number: Q6A0D4 Secondary accession number(s): Q3U654, Q3U8P6, Q80US1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2006 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 108 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families