UniProtKB - Q69ZT1 (FAN1_MOUSE)
Protein
Fanconi-associated nuclease 1
Gene
Fan1
Organism
Mus musculus (Mouse)
Status
Functioni
Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (By similarity). Not involved in DNA double-strand breaks resection. Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions (PubMed:24981866). Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL. Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap (By similarity). Also has endonuclease activity toward 5'-flaps (PubMed:24981866).By similarity1 Publication
Catalytic activityi
- Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.1 Publication EC:3.1.4.1
Cofactori
Mn2+By similarity, Mg2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 837 | Magnesium or manganese 2By similarity | 1 | |
Metal bindingi | 963 | Magnesium or manganese 1By similarity | 1 | |
Metal bindingi | 963 | Magnesium or manganese 2By similarity | 1 | |
Metal bindingi | 978 | Magnesium or manganese 1By similarity | 1 | |
Metal bindingi | 979 | Magnesium or manganese 1; via carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 41 – 69 | UBZ4-typePROSITE-ProRule annotationAdd BLAST | 29 |
GO - Molecular functioni
- 5'-3' exonuclease activity Source: UniProtKB
- 5'-flap endonuclease activity Source: UniProtKB
- flap-structured DNA binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- phosphodiesterase I activity Source: UniProtKB-EC
- ubiquitin-dependent protein binding Source: UniProtKB
GO - Biological processi
- DNA repair Source: UniProtKB
- double-strand break repair via homologous recombination Source: UniProtKB
- interstrand cross-link repair Source: UniProtKB
- nucleotide-excision repair Source: UniProtKB
- nucleotide-excision repair, DNA incision Source: UniProtKB
Keywordsi
Molecular function | Endonuclease, Exonuclease, Hydrolase, Nuclease |
Biological process | DNA damage, DNA repair |
Ligand | Magnesium, Manganese, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-6783310, Fanconi Anemia Pathway |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3045266, Fan1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2. Localizes to stalled replication forks via its UBZ4-type zinc finger.By similarity
Cytosol
- cytosol Source: MGI
Extracellular region or secreted
- intercellular bridge Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000311225 | 1 – 1020 | Fanconi-associated nuclease 1Add BLAST | 1020 |
Post-translational modificationi
Ubiquitinated and degraded during mitotic exit by the APC/C-Cdh1 complex.By similarity
Keywords - PTMi
Ubl conjugationProteomic databases
EPDi | Q69ZT1 |
PaxDbi | Q69ZT1 |
PeptideAtlasi | Q69ZT1 |
PRIDEi | Q69ZT1 |
PTM databases
iPTMneti | Q69ZT1 |
PhosphoSitePlusi | Q69ZT1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000033458, Expressed in quadriceps femoris and 54 other tissues |
Interactioni
Subunit structurei
Interacts with FANCD2 (when monoubiquitinated).
Interacts with FANCI, MLH1, MLH3 and PMS2.
By similarityGO - Molecular functioni
- ubiquitin-dependent protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 236987, 1 interactor |
STRINGi | 10090.ENSMUSP00000130012 |
Miscellaneous databases
RNActi | Q69ZT1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 898 – 1010 | VRR-NUCAdd BLAST | 113 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 673 – 737 | Sequence analysisAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 14 – 22 | D-box | 9 | |
Motifi | 212 – 214 | KEN box | 3 |
Domaini
The UBZ4-type zinc finger specifically binds monoubiquitinated FANCD2.By similarity
The KEN box and D-box are required for interaction with FZR1/CDH1 and essential for APC(CDH1)-mediated ubiquitination.By similarity
Sequence similaritiesi
Belongs to the FAN1 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 41 – 69 | UBZ4-typePROSITE-ProRule annotationAdd BLAST | 29 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2143, Eukaryota |
GeneTreei | ENSGT00390000018637 |
HOGENOMi | CLU_005116_3_0_1 |
InParanoidi | Q69ZT1 |
OMAi | FQTCPLD |
OrthoDBi | 1003565at2759 |
PhylomeDBi | Q69ZT1 |
TreeFami | TF312870 |
Family and domain databases
Gene3Di | 3.40.1350.10, 1 hit |
InterProi | View protein in InterPro IPR033315, Fan1-like IPR006642, Rad18_UBZ4 IPR011856, tRNA_endonuc-like_dom_sf IPR014883, VRR_NUC |
PANTHERi | PTHR15749, PTHR15749, 1 hit |
Pfami | View protein in Pfam PF08774, VRR_NUC, 1 hit |
SMARTi | View protein in SMART SM00990, VRR_NUC, 1 hit |
PROSITEi | View protein in PROSITE PS51908, ZF_UBZ4, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q69ZT1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPSQRKSPDQ KRPRRSLSTS KTAKSQCHSI TSYFNSAPPA KLACSTCHKM
60 70 80 90 100
VPRYDLIRHL DESCANNGVG DDVQVEPAQA GLMSPTVPTS DLPSGPLENV
110 120 130 140 150
TPQKLSPPKR SLISVQCGSK LGIQQQTSPY FKDALVSKDQ NELPNQSVEI
160 170 180 190 200
MPLGSLTSKL SRRYLNAKKS LAKNEGLASQ CPQTSPSTPG TSLTDNCPEM
210 220 230 240 250
EDKDEVLNSS QKENIYSCAP LKEENASEQK VKNNKITGDE SQKASCGEPA
260 270 280 290 300
LTPASAEHAS ILLSSDSTLV SNTKSSPGDT LVKQESARRA DVGLAEPLEV
310 320 330 340 350
RSHKEVQMTF DAAAKTLVSG EAESNGPTDV DMSDMTTWSN NQELVREAGS
360 370 380 390 400
VLHCPLEQGS SCGGPSETAQ LALSHPYYLR SFLVVLQALL GNEEDMKLFD
410 420 430 440 450
EQEKAIITRF YQLSASGQKL YVRLFQRKLT WIKMSKLEYE EIASDLTPVV
460 470 480 490 500
EELKDSGFLQ TESELQELSD VLELLSAPEL KALAKTFHLV SPGGQKQQLV
510 520 530 540 550
DAFHKLAKQR SVCTWGKTQP GIRAVILKRA KDLAGRSLRV CKGPRAVFAR
560 570 580 590 600
ILLLFSLTDS MEDEEAACGG QGQLSTVLLV NLGRMEFPQY TICRKTQIFR
610 620 630 640 650
DREDLIRYAA AAHMLSDISA AMASGNWEDA KELARSAKRD WEQLKSHPSL
660 670 680 690 700
RYHEALPPFL RCFTVGWIYT RISSRAVEVL ERLHMYEEAV KELENLLSQK
710 720 730 740 750
IYCPDSRGRW WDRLALNLHQ HLKRLEEAIR CIREGLADPH VRTGHRLSLY
760 770 780 790 800
QRAVRLRESP SCRKYKHLFS RLPEVAVGDV KHVTITGRLC PQHGMGKSVF
810 820 830 840 850
VMESGDGANP TTVLCSVEEL ALGYYRQSGF DQGIHGEGST FSTLCGLLLW
860 870 880 890 900
DIIFMDGIPD VFRNAYQASP LDLLTDSFFA SREQALEARL QLIHSAPAES
910 920 930 940 950
LRAWVGEAWQ AQQGRVASLV SWDRFTSLQQ AQDLVSCLGG PVLSGVCRRL
960 970 980 990 1000
AADFRHCRGG LPDLVVWNSQ SHHCKLVEVK GPSDRLSCKQ MIWLYELQKL
1010 1020
GADVEVCHVV AVGAKSKGLG
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029431 | 415 – 469 | ASGQK…LQELS → GTFGLLAFRASSSGLCAMGG PVSSLMTTTRSHCGSTAGWL SVSRRGPFKTSSCAW in isoform 4. 1 PublicationAdd BLAST | 55 | |
Alternative sequenceiVSP_029432 | 462 – 468 | ESELQEL → GTVRRRT in isoform 3. 1 Publication | 7 | |
Alternative sequenceiVSP_029433 | 469 – 1020 | Missing in isoform 3. 1 PublicationAdd BLAST | 552 | |
Alternative sequenceiVSP_029434 | 470 – 1020 | Missing in isoform 4. 1 PublicationAdd BLAST | 551 | |
Alternative sequenceiVSP_029435 | 728 – 743 | AIRCI…PHVRT → VLAAYPAAYLVLNGKL in isoform 2. 1 PublicationAdd BLAST | 16 | |
Alternative sequenceiVSP_029436 | 744 – 1020 | Missing in isoform 2. 1 PublicationAdd BLAST | 277 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK077918 mRNA Translation: BAC37062.1 AC129199 Genomic DNA No translation available. AC139849 Genomic DNA No translation available. BC116270 mRNA Translation: AAI16271.1 BC116271 mRNA Translation: AAI16272.1 AK173087 mRNA Translation: BAD32365.1 |
CCDSi | CCDS52265.1 [Q69ZT1-1] |
RefSeqi | NP_808561.2, NM_177893.3 [Q69ZT1-1] XP_006541021.1, XM_006540958.3 [Q69ZT1-1] |
Genome annotation databases
Ensembli | ENSMUST00000163289; ENSMUSP00000130012; ENSMUSG00000033458 [Q69ZT1-1] |
GeneIDi | 330554 |
KEGGi | mmu:330554 |
UCSCi | uc009hge.1, mouse [Q69ZT1-2] uc009hgf.2, mouse [Q69ZT1-3] uc012fmp.1, mouse [Q69ZT1-1] uc029wgg.1, mouse [Q69ZT1-4] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK077918 mRNA Translation: BAC37062.1 AC129199 Genomic DNA No translation available. AC139849 Genomic DNA No translation available. BC116270 mRNA Translation: AAI16271.1 BC116271 mRNA Translation: AAI16272.1 AK173087 mRNA Translation: BAD32365.1 |
CCDSi | CCDS52265.1 [Q69ZT1-1] |
RefSeqi | NP_808561.2, NM_177893.3 [Q69ZT1-1] XP_006541021.1, XM_006540958.3 [Q69ZT1-1] |
3D structure databases
SMRi | Q69ZT1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 236987, 1 interactor |
STRINGi | 10090.ENSMUSP00000130012 |
PTM databases
iPTMneti | Q69ZT1 |
PhosphoSitePlusi | Q69ZT1 |
Proteomic databases
EPDi | Q69ZT1 |
PaxDbi | Q69ZT1 |
PeptideAtlasi | Q69ZT1 |
PRIDEi | Q69ZT1 |
Protocols and materials databases
Antibodypediai | 22515, 108 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000163289; ENSMUSP00000130012; ENSMUSG00000033458 [Q69ZT1-1] |
GeneIDi | 330554 |
KEGGi | mmu:330554 |
UCSCi | uc009hge.1, mouse [Q69ZT1-2] uc009hgf.2, mouse [Q69ZT1-3] uc012fmp.1, mouse [Q69ZT1-1] uc029wgg.1, mouse [Q69ZT1-4] |
Organism-specific databases
CTDi | 22909 |
MGIi | MGI:3045266, Fan1 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG2143, Eukaryota |
GeneTreei | ENSGT00390000018637 |
HOGENOMi | CLU_005116_3_0_1 |
InParanoidi | Q69ZT1 |
OMAi | FQTCPLD |
OrthoDBi | 1003565at2759 |
PhylomeDBi | Q69ZT1 |
TreeFami | TF312870 |
Enzyme and pathway databases
Reactomei | R-MMU-6783310, Fanconi Anemia Pathway |
Miscellaneous databases
BioGRID-ORCSi | 330554, 0 hits in 17 CRISPR screens |
PROi | PR:Q69ZT1 |
RNActi | Q69ZT1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000033458, Expressed in quadriceps femoris and 54 other tissues |
Family and domain databases
Gene3Di | 3.40.1350.10, 1 hit |
InterProi | View protein in InterPro IPR033315, Fan1-like IPR006642, Rad18_UBZ4 IPR011856, tRNA_endonuc-like_dom_sf IPR014883, VRR_NUC |
PANTHERi | PTHR15749, PTHR15749, 1 hit |
Pfami | View protein in Pfam PF08774, VRR_NUC, 1 hit |
SMARTi | View protein in SMART SM00990, VRR_NUC, 1 hit |
PROSITEi | View protein in PROSITE PS51908, ZF_UBZ4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FAN1_MOUSE | |
Accessioni | Q69ZT1Primary (citable) accession number: Q69ZT1 Secondary accession number(s): Q14B88, Q8BVK2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | November 13, 2007 | |
Last modified: | February 10, 2021 | |
This is version 114 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families