UniProtKB - Q69ZN6 (GNPTA_MOUSE)
N-acetylglucosamine-1-phosphotransferase subunits alpha/beta
Gnptab
Functioni
Miscellaneous
Catalytic activityi
- N4-[α-D-mannosyl-(1→2)-α-D-mannosyl-(glycan)]-L-asparaginyl-[protein] + UDP-N-acetyl-α-D-glucosamine = H+ + N4-[6-(N-acetyl-α-D-glucosaminyl-1-phospho)-α-D-mannosyl-(1→2)-α-D-mannosyl-(glycan)]-L-asparaginyl-[protein] + UMPBy similarityEC:2.7.8.17By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 449 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 464 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 467 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 516 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 531 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 534 | CalciumPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 997 – 1008 | PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity Source: UniProtKB
GO - Biological processi
- carbohydrate phosphorylation Source: MGI
- lysosome organization Source: UniProtKB
- N-glycan processing to lysosome Source: UniProtKB
- protein secretion Source: MGI
- secretion of lysosomal enzymes Source: MGI
Keywordsi
Molecular function | Transferase |
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (EC:2.7.8.17By similarity)Alternative name(s): GlcNAc-1-phosphotransferase subunits alpha/beta Stealth protein GNPTAB UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta Cleaved into the following 2 chains: |
Gene namesi | Name:Gnptab Synonyms:Gnpta, Kiaa1208 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3643902, Gnptab |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 22 – 42 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1194 – 1214 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000225010 | 1 – 907 | N-acetylglucosamine-1-phosphotransferase subunit alphaAdd BLAST | 907 | |
ChainiPRO_0000225011 | 908 – 1235 | N-acetylglucosamine-1-phosphotransferase subunit betaAdd BLAST | 328 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 83 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 114 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 148 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 179 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 438 ↔ 461 | PROSITE-ProRule annotation | ||
Disulfide bondi | 452 ↔ 468 | PROSITE-ProRule annotation | ||
Disulfide bondi | 505 ↔ 528 | PROSITE-ProRule annotation | ||
Disulfide bondi | 519 ↔ 535 | PROSITE-ProRule annotation | ||
Glycosylationi | 614 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 729 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 988 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1108 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 907 – 908 | Cleavage; by MBTPS1By similarity | 2 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q69ZN6 |
PeptideAtlasi | Q69ZN6 |
PRIDEi | Q69ZN6 |
PTM databases
GlyGeni | Q69ZN6, 8 sites |
iPTMneti | Q69ZN6 |
PhosphoSitePlusi | Q69ZN6 |
SwissPalmi | Q69ZN6 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000035311, Expressed in forelimb bud and 287 other tissues |
Genevisiblei | Q69ZN6, MM |
Interactioni
Subunit structurei
Hexamer of two alpha, two beta and two gamma (GNPTG) subunits; disulfide-linked. The alpha and/or the beta subunits of the enzyme constitute the catalytic subunits.
By similarityProtein-protein interaction databases
BioGRIDi | 240639, 1 interactor |
STRINGi | 10090.ENSMUSP00000020251 |
Miscellaneous databases
RNActi | Q69ZN6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 438 – 473 | LNR 1Add BLAST | 36 | |
Repeati | 505 – 545 | LNR 2Add BLAST | 41 | |
Domaini | 699 – 823 | DMAP1-bindingPROSITE-ProRule annotationAdd BLAST | 125 | |
Domaini | 984 – 1019 | EF-handPROSITE-ProRule annotationAdd BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 480 – 485 | Poly-Gly | 6 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QQMR, Eukaryota |
GeneTreei | ENSGT00390000006747 |
HOGENOMi | CLU_002469_0_0_1 |
InParanoidi | Q69ZN6 |
OMAi | KRHDVNS |
OrthoDBi | 851009at2759 |
PhylomeDBi | Q69ZN6 |
TreeFami | TF324175 |
Family and domain databases
InterProi | View protein in InterPro IPR010506, DMAP1-bd IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR041536, GNPTAB_reg IPR035993, Notch-like_dom_sf IPR000800, Notch_dom IPR031358, Stealth_CR1 IPR021520, Stealth_CR2 IPR031357, Stealth_CR3 IPR031356, Stealth_CR4 |
Pfami | View protein in Pfam PF06464, DMAP_binding, 1 hit PF18440, GlcNAc-1_reg, 1 hit PF00066, Notch, 2 hits PF17101, Stealth_CR1, 1 hit PF11380, Stealth_CR2, 1 hit PF17102, Stealth_CR3, 1 hit PF17103, Stealth_CR4, 1 hit |
SMARTi | View protein in SMART SM01137, DMAP_binding, 1 hit SM00004, NL, 2 hits |
SUPFAMi | SSF90193, SSF90193, 1 hit |
PROSITEi | View protein in PROSITE PS51912, DMAP1_BIND, 1 hit PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit PS50258, LNR, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLLKLLQRQT YTCLSHRYGL YVCFVGVVVT IVSAFQFGEV VLEWSRDQYH
60 70 80 90 100
VLFDSYRDNI AGKSFQNRLC LPMPIDVVYT WVNGTDLELL KELQQVREHM
110 120 130 140 150
EEEQRAMRET LGKNTTEPTK KSEKQLECLL THCIKVPMLV LDPPLPANCT
160 170 180 190 200
LKDLPTLYPS FHAASDMFNV AKPKNPSTNV SVVVFDTTKD VEDAHAGPFK
210 220 230 240 250
GGSKQMVWRA YLTTDKEAPG LVLMQGLAFL SGFPPTFKET SQLKTKLPEK
260 270 280 290 300
LSSKIKLLRL YSEASVALLK LNNPKGFQEL NKQTKKNMTI DGKELTISPA
310 320 330 340 350
YLLWDLSAIS QSKQDEDVSA SRFEDNEELR YSLRSIERHA PWVRNIFIVT
360 370 380 390 400
NGQIPSWLNL DNPRVTIVTH QDIFQNLSHL PTFSSPAIES HIHRIEGLSQ
410 420 430 440 450
KFIYLNDDVM FGKDVWPDDF YSHSKGQKVY LTWPVPNCAE GCPGSWIKDG
460 470 480 490 500
YCDKACNNSA CDWDGGDCSG NTAGNRFVAG GGGTGNIGAG QHWQFGGGIN
510 520 530 540 550
TISYCNQGCA NSWLADKFCD QACNVLSCGF DAGDCGQDHF HELYKVTLLP
560 570 580 590 600
NQTHYVVPKG EYLSYFSFAN IARRGVEGTY SDNPIIRHAS IANKWKTIHL
610 620 630 640 650
IMHSGMNATT IYFNLTLQNA NDEEFKIQIA VEVDTREAPK LNSTTQKAYE
660 670 680 690 700
SLVSPVTPLP QADVPFEDVP KEKRFPKIRR HDVNATGRFQ EEVKIPRVNI
710 720 730 740 750
SLLPKEAQVR LSNLDLQLER GDITLKGYNL SKSALLRSFL GNSLDTKIKP
760 770 780 790 800
QARTDETKGN LEVPQENPSH RRPHGFAGEH RSERWTAPAE TVTVKGRDHA
810 820 830 840 850
LNPPPVLETN ARLAQPTLGV TVSKENLSPL IVPPESHLPK EEESDRAEGN
860 870 880 890 900
AVPVKELVPG RRLQQNYPGF LPWEKKKYFQ DLLDEEESLK TQLAYFTDSK
910 920 930 940 950
HTGRQLKDTF ADSLRYVNKI LNSKFGFTSR KVPAHMPHMI DRIVMQELQD
960 970 980 990 1000
MFPEEFDKTS FHKVRHSEDM QFAFSYFYYL MSAVQPLNIS QVFHEVDTDQ
1010 1020 1030 1040 1050
SGVLSDREIR TLATRIHDLP LSLQDLTGLE HMLINCSKML PANITQLNNI
1060 1070 1080 1090 1100
PPTQEAYYDP NLPPVTKSLV TNCKPVTDKI HKAYKDKNKY RFEIMGEEEI
1110 1120 1130 1140 1150
AFKMIRTNVS HVVGQLDDIR KNPRKFVCLN DNIDHNHKDA RTVKAVLRDF
1160 1170 1180 1190 1200
YESMFPIPSQ FELPREYRNR FLHMHELQEW RAYRDKLKFW THCVLATLII
1210 1220 1230
FTIFSFFAEQ IIALKRKIFP RRRIHKEASP DRIRV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YXC6 | D3YXC6_MOUSE | N-acetylglucosamine-1-phosphotransf... | Gnptab | 166 | Annotation score: | ||
D6RJ30 | D6RJ30_MOUSE | N-acetylglucosamine-1-phosphotransf... | Gnptab | 56 | Annotation score: | ||
D3Z1C3 | D3Z1C3_MOUSE | N-acetylglucosamine-1-phosphotransf... | Gnptab | 174 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 444 | G → S in BAE24503 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 707 | A → V in BAE32779 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017340 | 1 – 46 | Missing in isoform 2. 1 PublicationAdd BLAST | 46 | |
Alternative sequenceiVSP_017341 | 47 – 67 | DQYHV…KSFQN → MGSTSASWASLSPSSRLSSS E in isoform 2. 1 PublicationAdd BLAST | 21 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK173132 mRNA Translation: BAD32410.1 Different initiation. AK140867 mRNA Translation: BAE24503.1 AK154710 mRNA Translation: BAE32779.1 Different initiation. |
CCDSi | CCDS24110.1 [Q69ZN6-1] |
RefSeqi | NP_001004164.2, NM_001004164.2 [Q69ZN6-1] |
Genome annotation databases
Ensembli | ENSMUST00000020251; ENSMUSP00000020251; ENSMUSG00000035311 [Q69ZN6-1] |
GeneIDi | 432486 |
KEGGi | mmu:432486 |
UCSCi | uc007grk.1, mouse [Q69ZN6-1] uc011xlg.1, mouse [Q69ZN6-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK173132 mRNA Translation: BAD32410.1 Different initiation. AK140867 mRNA Translation: BAE24503.1 AK154710 mRNA Translation: BAE32779.1 Different initiation. |
CCDSi | CCDS24110.1 [Q69ZN6-1] |
RefSeqi | NP_001004164.2, NM_001004164.2 [Q69ZN6-1] |
3D structure databases
SMRi | Q69ZN6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 240639, 1 interactor |
STRINGi | 10090.ENSMUSP00000020251 |
PTM databases
GlyGeni | Q69ZN6, 8 sites |
iPTMneti | Q69ZN6 |
PhosphoSitePlusi | Q69ZN6 |
SwissPalmi | Q69ZN6 |
Proteomic databases
PaxDbi | Q69ZN6 |
PeptideAtlasi | Q69ZN6 |
PRIDEi | Q69ZN6 |
Protocols and materials databases
Antibodypediai | 44958, 77 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000020251; ENSMUSP00000020251; ENSMUSG00000035311 [Q69ZN6-1] |
GeneIDi | 432486 |
KEGGi | mmu:432486 |
UCSCi | uc007grk.1, mouse [Q69ZN6-1] uc011xlg.1, mouse [Q69ZN6-2] |
Organism-specific databases
CTDi | 79158 |
MGIi | MGI:3643902, Gnptab |
Rougei | Search... |
Phylogenomic databases
eggNOGi | ENOG502QQMR, Eukaryota |
GeneTreei | ENSGT00390000006747 |
HOGENOMi | CLU_002469_0_0_1 |
InParanoidi | Q69ZN6 |
OMAi | KRHDVNS |
OrthoDBi | 851009at2759 |
PhylomeDBi | Q69ZN6 |
TreeFami | TF324175 |
Miscellaneous databases
BioGRID-ORCSi | 432486, 0 hits in 17 CRISPR screens |
ChiTaRSi | Gnptab, mouse |
PROi | PR:Q69ZN6 |
RNActi | Q69ZN6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000035311, Expressed in forelimb bud and 287 other tissues |
Genevisiblei | Q69ZN6, MM |
Family and domain databases
InterProi | View protein in InterPro IPR010506, DMAP1-bd IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR041536, GNPTAB_reg IPR035993, Notch-like_dom_sf IPR000800, Notch_dom IPR031358, Stealth_CR1 IPR021520, Stealth_CR2 IPR031357, Stealth_CR3 IPR031356, Stealth_CR4 |
Pfami | View protein in Pfam PF06464, DMAP_binding, 1 hit PF18440, GlcNAc-1_reg, 1 hit PF00066, Notch, 2 hits PF17101, Stealth_CR1, 1 hit PF11380, Stealth_CR2, 1 hit PF17102, Stealth_CR3, 1 hit PF17103, Stealth_CR4, 1 hit |
SMARTi | View protein in SMART SM01137, DMAP_binding, 1 hit SM00004, NL, 2 hits |
SUPFAMi | SSF90193, SSF90193, 1 hit |
PROSITEi | View protein in PROSITE PS51912, DMAP1_BIND, 1 hit PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit PS50258, LNR, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNPTA_MOUSE | |
Accessioni | Q69ZN6Primary (citable) accession number: Q69ZN6 Secondary accession number(s): Q3U3K6, Q3US34 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 7, 2006 |
Last sequence update: | March 7, 2006 | |
Last modified: | December 2, 2020 | |
This is version 120 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families