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Protein

Neuroligin-2

Gene

Nlgn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transmembrane scaffolding protein involved in cell-cell interactions via its interactions with neurexin family members. Mediates cell-cell interactions both in neurons and in other types of cells, such as Langerhans beta cells. Mediates cell-cell interactions between Langerhans beta cells and modulates insulin secretion (By similarity). Plays a role in synapse function and synaptic signal transmission, especially via gamma-aminobutyric acid receptors (GABA(A) receptors). Functions by recruiting and clustering synaptic proteins. Promotes clustering of postsynaptic GABRG2 and GPHN. Promotes clustering of postsynaptic LHFPL4 (PubMed:29742426). Modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing. Required for normal signal amplitude from inhibitory synapses, but is not essential for normal signal frequency. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures.By similarity7 Publications

GO - Molecular functioni

  • cell adhesion molecule binding Source: BHF-UCL
  • identical protein binding Source: IntAct
  • neurexin family protein binding Source: BHF-UCL
  • signaling receptor activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-6794361 Neurexins and neuroligins

Protein family/group databases

ESTHERimouse-2neur Neuroligin

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroligin-2
Gene namesi
Name:Nlgn2
Synonyms:Kiaa1366
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2681835 Nlgn2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini15 – 678ExtracellularSequence analysisAdd BLAST664
Transmembranei679 – 699HelicalSequence analysisAdd BLAST21
Topological domaini700 – 836CytoplasmicSequence analysisAdd BLAST137

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype, but mice present subtle behavorial changes. Signaling from inhibitory synapses is impaired. In addition, mice have reduced brain volume. Mice lacking both NLGN1 and NLGN2, or NLGN2 and NLGN3, are viable, but have impaired breathing, drastically reduced reproduction rates and striking deficits in raising their offspring. Mice lacking NLGN1, NLGN2 and NLGN3 are born at the expected Mendelian rate, but die shortly after birth due to respiratory failure. They do not show a significant change in the number of synapses, but synapse function is strongly impaired.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 14By similarityAdd BLAST14
ChainiPRO_000004187915 – 836Neuroligin-2Add BLAST822

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi98N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi106 ↔ 1411 Publication
Glycosylationi136N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi317 ↔ 3281 Publication
Disulfide bondi487 ↔ 5211 Publication
Glycosylationi522N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei714PhosphoserineCombined sources1
Modified residuei719PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ69ZK9
PaxDbiQ69ZK9
PeptideAtlasiQ69ZK9
PRIDEiQ69ZK9

PTM databases

iPTMnetiQ69ZK9
PhosphoSitePlusiQ69ZK9

Expressioni

Tissue specificityi

Brain and arteries. Detected in the retina outer plexiform layer (at protein level). Widely expressed. Detected in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.5 Publications

Gene expression databases

BgeeiENSMUSG00000051790 Expressed in 240 organ(s), highest expression level in subiculum
CleanExiMM_NLGN2
ExpressionAtlasiQ69ZK9 baseline and differential
GenevisibleiQ69ZK9 MM

Interactioni

Subunit structurei

Interacts with NRXN1, NRXN2 and NRXN3. Interacts (via its C-terminus) with DLG4/PSD-95 (via PDZ domain 3). Interacts with PATJ (By similarity). Interacts with MDGA2 (By similarity). Interacts with GPHN (PubMed:19755106). Interacts with MDGA1 (PubMed:23358245). Found in a complex with MAGI2 and IGSF9B, where it interacts with MAGI2 (via WW 1, WW 2 and PDZ 2 domains) (PubMed:23751499). Identified in a complex of 720 kDa composed of LHFPL4, NLGN2, GABRA1, GABRB2, GABRG2 and GABRB3 (By similarity). Interacts with LHFPL4; leading to mutual regulation of the protein level and synaptic clustering (PubMed:29742426, PubMed:28279354). Interacts with GABRA1 (By similarity).By similarity5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229806, 3 interactors
DIPiDIP-29702N
IntActiQ69ZK9, 4 interactors
MINTiQ69ZK9
STRINGi10090.ENSMUSP00000053097

Structurei

Secondary structure

1836
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ69ZK9
SMRiQ69ZK9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ69ZK9

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni679 – 699Required for interaction with LHFPL41 PublicationAdd BLAST21

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1516 Eukaryota
COG2272 LUCA
GeneTreeiENSGT00760000118946
HOGENOMiHOG000231424
HOVERGENiHBG008839
InParanoidiQ69ZK9
KOiK07378
OMAiGMKPRMK
OrthoDBiEOG091G0CHW
PhylomeDBiQ69ZK9
TreeFamiTF326187

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002018 CarbesteraseB
IPR019819 Carboxylesterase_B_CS
IPR000460 Nlgn
IPR030023 NLGN2
PANTHERiPTHR43903:SF3 PTHR43903:SF3, 1 hit
PfamiView protein in Pfam
PF00135 COesterase, 1 hit
PRINTSiPR01090 NEUROLIGIN
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00941 CARBOXYLESTERASE_B_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q69ZK9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWLLALCLVG LAGAQRGGGG PGGGAPGGPG LGLGSLGEER FPVVNTAYGR
60 70 80 90 100
VRGVRRELNN EILGPVVQFL GVPYATPPLG ARRFQPPEAP ASWPGVRNAT
110 120 130 140 150
TLPPACPQNL HGALPAIMLP VWFTDNLEAA ATYVQNQSED CLYLNLYVPT
160 170 180 190 200
EDGPLTKKRD EATLNPPDTD IRDSGKKPVM LFLHGGSYME GTGNMFDGSV
210 220 230 240 250
LAAYGNVIVV TLNYRLGVLG FLSTGDQAAK GNYGLLDQIQ ALRWLSENIA
260 270 280 290 300
HFGGDPERIT IFGSGAGASC VNLLILSHHS EGLFQKAIAQ SGTAISSWSV
310 320 330 340 350
NYQPLKYTRL LAAKVGCDRE DSTEAVECLR RKSSRELVDQ DVQPARYHIA
360 370 380 390 400
FGPVVDGDVV PDDPEILMQQ GEFLNYDMLI GVNQGEGLKF VEDSAESEDG
410 420 430 440 450
VSASAFDFTV SNFVDNLYGY PEGKDVLRET IKFMYTDWAD RDNGEMRRKT
460 470 480 490 500
LLALFTDHQW VAPAVATAKL HADYQSPVYF YTFYHHCQAE GRPEWADAAH
510 520 530 540 550
GDELPYVFGV PMVGATDLFP CNFSKNDVML SAVVMTYWTN FAKTGDPNQP
560 570 580 590 600
VPQDTKFIHT KPNRFEEVVW SKFNSKEKQY LHIGLKPRVR DNYRANKVAF
610 620 630 640 650
WLELVPHLHN LHTELFTTTT RLPPYATRWP PRTPGPGTSG TRRPPPPATL
660 670 680 690 700
PPESDIDLGP RAYDRFPGDS RDYSTELSVT VAVGASLLFL NILAFAALYY
710 720 730 740 750
KRDRRQELRC RRLSPPGGSG SGVPGGGPLL PTAGRELPPE EELVSLQLKR
760 770 780 790 800
GGGVGADPAE ALRPACPPDY TLALRRAPDD VPLLAPGALT LLPSGLGPPP
810 820 830
PPPPPSLHPF GPFPPPPPTA TSHNNTLPHP HSTTRV
Length:836
Mass (Da):90,989
Last modified:September 13, 2005 - v2
Checksum:i4C03297F4F1A1F14
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6VE93F6VE93_MOUSE
Neuroligin-2
Nlgn2
262Annotation score:

Sequence cautioni

The sequence BAD32437 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti210V → A in BAD32437 (PubMed:15368895).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173159 mRNA Translation: BAD32437.1 Different initiation.
AL603707 Genomic DNA No translation available.
CCDSiCCDS24914.1
RefSeqiNP_942562.2, NM_198862.2
XP_006532964.1, XM_006532901.2
UniGeneiMm.151293

Genome annotation databases

EnsembliENSMUST00000056484; ENSMUSP00000053097; ENSMUSG00000051790
ENSMUST00000108634; ENSMUSP00000104274; ENSMUSG00000051790
GeneIDi216856
KEGGimmu:216856
UCSCiuc007jrw.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173159 mRNA Translation: BAD32437.1 Different initiation.
AL603707 Genomic DNA No translation available.
CCDSiCCDS24914.1
RefSeqiNP_942562.2, NM_198862.2
XP_006532964.1, XM_006532901.2
UniGeneiMm.151293

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BL8X-ray3.30A/B/C/D42-612[»]
ProteinModelPortaliQ69ZK9
SMRiQ69ZK9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229806, 3 interactors
DIPiDIP-29702N
IntActiQ69ZK9, 4 interactors
MINTiQ69ZK9
STRINGi10090.ENSMUSP00000053097

Protein family/group databases

ESTHERimouse-2neur Neuroligin

PTM databases

iPTMnetiQ69ZK9
PhosphoSitePlusiQ69ZK9

Proteomic databases

MaxQBiQ69ZK9
PaxDbiQ69ZK9
PeptideAtlasiQ69ZK9
PRIDEiQ69ZK9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056484; ENSMUSP00000053097; ENSMUSG00000051790
ENSMUST00000108634; ENSMUSP00000104274; ENSMUSG00000051790
GeneIDi216856
KEGGimmu:216856
UCSCiuc007jrw.1 mouse

Organism-specific databases

CTDi57555
MGIiMGI:2681835 Nlgn2
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1516 Eukaryota
COG2272 LUCA
GeneTreeiENSGT00760000118946
HOGENOMiHOG000231424
HOVERGENiHBG008839
InParanoidiQ69ZK9
KOiK07378
OMAiGMKPRMK
OrthoDBiEOG091G0CHW
PhylomeDBiQ69ZK9
TreeFamiTF326187

Enzyme and pathway databases

ReactomeiR-MMU-6794361 Neurexins and neuroligins

Miscellaneous databases

ChiTaRSiNlgn2 mouse
EvolutionaryTraceiQ69ZK9
PROiPR:Q69ZK9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051790 Expressed in 240 organ(s), highest expression level in subiculum
CleanExiMM_NLGN2
ExpressionAtlasiQ69ZK9 baseline and differential
GenevisibleiQ69ZK9 MM

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002018 CarbesteraseB
IPR019819 Carboxylesterase_B_CS
IPR000460 Nlgn
IPR030023 NLGN2
PANTHERiPTHR43903:SF3 PTHR43903:SF3, 1 hit
PfamiView protein in Pfam
PF00135 COesterase, 1 hit
PRINTSiPR01090 NEUROLIGIN
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00941 CARBOXYLESTERASE_B_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNLGN2_MOUSE
AccessioniPrimary (citable) accession number: Q69ZK9
Secondary accession number(s): Q5F288
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: November 7, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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