UniProtKB - Q69Z28 (ATS16_MOUSE)
Protein
A disintegrin and metalloproteinase with thrombospondin motifs 16
Gene
Adamts16
Organism
Mus musculus (Mouse)
Status
Functioni
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 247 | Zinc; in inhibited formBy similarity | 1 | |
Metal bindingi | 431 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 432 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 435 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 441 | Zinc; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- metalloendopeptidase activity Source: GO_Central
GO - Biological processi
- branching involved in ureteric bud morphogenesis Source: MGI
- extracellular matrix organization Source: GO_Central
- male gamete generation Source: MGI
- regulation of cilium assembly Source: MGI
- regulation of systemic arterial blood pressure Source: MGI
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-5173214, O-glycosylation of TSR domain-containing proteins |
Protein family/group databases
MEROPSi | M12.026 |
Names & Taxonomyi
Protein namesi | Recommended name: A disintegrin and metalloproteinase with thrombospondin motifs 16 (EC:3.4.24.-)Short name: ADAM-TS 16 Short name: ADAM-TS16 Short name: ADAMTS-16 |
Gene namesi | Name:Adamts16 Synonyms:Kiaa2029 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2429637, Adamts16 |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
Extracellular region or secreted
- extracellular matrix Source: GO_Central
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
PropeptideiPRO_0000029196 | 21 – 277 | By similarityAdd BLAST | 257 | |
ChainiPRO_0000029197 | 278 – 1222 | A disintegrin and metalloproteinase with thrombospondin motifs 16Add BLAST | 945 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 154 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 190 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 308 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 364 ↔ 415 | By similarity | ||
Disulfide bondi | 390 ↔ 397 | By similarity | ||
Disulfide bondi | 409 ↔ 488 | By similarity | ||
Disulfide bondi | 448 ↔ 472 | By similarity | ||
Disulfide bondi | 516 ↔ 541 | By similarity | ||
Disulfide bondi | 527 ↔ 548 | By similarity | ||
Disulfide bondi | 536 ↔ 567 | By similarity | ||
Disulfide bondi | 561 ↔ 572 | By similarity | ||
Disulfide bondi | 596 ↔ 633 | By similarity | ||
Disulfide bondi | 600 ↔ 638 | By similarity | ||
Disulfide bondi | 611 ↔ 623 | By similarity | ||
Glycosylationi | 739 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 778 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 825 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 833 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 903 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 933 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
The precursor is cleaved by a furin endopeptidase.By similarity
Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).By similarity
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | Q69Z28 |
PRIDEi | Q69Z28 |
PTM databases
GlyGeni | Q69Z28, 9 sites |
iPTMneti | Q69Z28 |
PhosphoSitePlusi | Q69Z28 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000049538, Expressed in efferent duct and 134 other tissues |
Genevisiblei | Q69Z28, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 234828, 1 interactor |
STRINGi | 10090.ENSMUSP00000079041 |
Miscellaneous databases
RNActi | Q69Z28, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 288 – 493 | Peptidase M12BPROSITE-ProRule annotationAdd BLAST | 206 | |
Domaini | 494 – 583 | DisintegrinAdd BLAST | 90 | |
Domaini | 584 – 639 | TSP type-1 1PROSITE-ProRule annotationAdd BLAST | 56 | |
Domaini | 872 – 920 | TSP type-1 2PROSITE-ProRule annotationAdd BLAST | 49 | |
Domaini | 925 – 985 | TSP type-1 3PROSITE-ProRule annotationAdd BLAST | 61 | |
Domaini | 986 – 1046 | TSP type-1 4PROSITE-ProRule annotationAdd BLAST | 61 | |
Domaini | 1049 – 1113 | TSP type-1 5PROSITE-ProRule annotationAdd BLAST | 65 | |
Domaini | 1125 – 1179 | TSP type-1 6PROSITE-ProRule annotationAdd BLAST | 55 | |
Domaini | 1184 – 1221 | PLACPROSITE-ProRule annotationAdd BLAST | 38 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 745 – 871 | SpacerAdd BLAST | 127 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 245 – 253 | Cysteine switchBy similarity | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 640 – 744 | Cys-richAdd BLAST | 105 |
Domaini
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.By similarity
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG3538, Eukaryota |
GeneTreei | ENSGT00940000159433 |
HOGENOMi | CLU_000660_1_0_1 |
InParanoidi | Q69Z28 |
OMAi | DFHMDLK |
OrthoDBi | 125522at2759 |
PhylomeDBi | Q69Z28 |
TreeFami | TF313537 |
Family and domain databases
Gene3Di | 2.20.100.10, 5 hits 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR041645, ADAM_CR_2 IPR010294, ADAM_spacer1 IPR013273, ADAMTS/ADAMTS-like IPR024079, MetalloPept_cat_dom_sf IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR010909, PLAC IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF17771, ADAM_CR_2, 1 hit PF05986, ADAM_spacer1, 1 hit PF01562, Pep_M12B_propep, 1 hit PF08686, PLAC, 1 hit PF01421, Reprolysin, 1 hit PF00090, TSP_1, 1 hit |
PRINTSi | PR01857, ADAMTSFAMILY |
SMARTi | View protein in SMART SM00209, TSP1, 6 hits |
SUPFAMi | SSF82895, SSF82895, 5 hits |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50900, PLAC, 1 hit PS50092, TSP1, 5 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q69Z28-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MESRGCAALW VLLLAQVSEQ QTPACALGLA AAASGSPEDP QPPPFSGSSW
60 70 80 90 100
LETGEYDLVS AYEVDHRGDY VSHDIMHYQR RRRRRAVTQP GGDALHLRLK
110 120 130 140 150
GPRHDLHLDL KAASNLMAPG FMVQTLGKGG TKSVQMFPPE ENCFYQGSLR
160 170 180 190 200
SQGNSSVALS TCQGLLGMIR TKDTDYFLKP LPPHLTSKLN RSAQGDSPSH
210 220 230 240 250
VLYKRSTERQ APRENEVLMI TRKRDLARPH LHHDNFHLGP SQKQHFCGRR
260 270 280 290 300
KKYMPQPPND DLYILPDEYK PSSRHKRSLL KSHRNEELNV ETLVVVDRKM
310 320 330 340 350
MQSHGHENIT TYVLTILNMV SALFKDGTIG GNINIVIVGL ILLEDEQPGL
360 370 380 390 400
AISHHADHTL TSFCQWQSGL MGKDGTRHDH AILLTGLDIC SWKNEPCDTL
410 420 430 440 450
GFAPISGMCS KYRSCTVNED SGLGLAFTIA HESGHNFGMV HDGEGNMCKK
460 470 480 490 500
SEGNIMSPTL AGRNGVFSWS SCSRQYLHKF LSTAQAICLA DQPKPVKEYK
510 520 530 540 550
YPEKLPGQLY DANTQCKWQF GEKAKLCMLD FRKDICKALW CHRIGRKCET
560 570 580 590 600
KFMPAAEGTL CGQDMWCRGG QCVKYGDEGP KPTHGHWSDW SPWSPCSRTC
610 620 630 640 650
GGGISHRDRL CTNPRPSHGG KFCQGSTRTL KLCNSQRCPL DSVDFRAAQC
660 670 680 690 700
AEYNSKRFRG WLYKWKPYTQ LEDQDLCKLY CIAEGFDFFF SLSNKVKDGT
710 720 730 740 750
PCSEDSRNVC IDGMCERVGC DNVLGSDATE DSCGVCKGNN SDCVTHRGLY
760 770 780 790 800
SKHHSTNQYY HMVTIPSGAR SIHIYETNIS TSYISVRNSL KRYYLNGHWS
810 820 830 840 850
VDWPGRYKFS GATFNYKRSY KEPENLTSPG PTNETLIVEL LFQGRNPGVA
860 870 880 890 900
WEFSLPRSGA KKTPAAQPSY SWAIVRSECS VSCGGGKMNS KAGCYRDLKV
910 920 930 940 950
PVNASFCNPK TRPVTGLVPC KVSPCPSSWS VGNWSVCSRT CGGGTQSRPV
960 970 980 990 1000
RCTRRAHYRD ESIPASLCPQ PEPPIHQACN SQSCPPAWST GPWAECSRTC
1010 1020 1030 1040 1050
GKGWRKRTVA CKSTNPSARA QLLHDTACTS EPKPRTHEIC LLKRCHKHKK
1060 1070 1080 1090 1100
LQWLVSAWSQ CSVTCQGGTQ QRVLRCAEKY ISGKYRELAS KKCLHLPKPD
1110 1120 1130 1140 1150
LELERACGLI PCPKHPPFDA SGSPRGSWFA SPWSQCTASC GGGVQRRTVQ
1160 1170 1180 1190 1200
CLLRGQPASD CFLHEKPETS SACNTHFCPI AEKRGTFCKD LFHWCYLVPQ
1210 1220
HGMCGHRFYS KQCCNTCSKS NL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD6RGS8 | D6RGS8_MOUSE | A disintegrin and metalloproteinase... | Adamts16 | 684 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_014991 | 886 – 900 | GKMNS…RDLKV → DRQCQTGRGHLEISS in isoform 2. 1 PublicationAdd BLAST | 15 | |
Alternative sequenceiVSP_014992 | 901 – 1222 | Missing in isoform 2. 1 PublicationAdd BLAST | 322 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK173338 mRNA Translation: BAD32616.1 BC034739 mRNA Translation: AAH34739.1 |
CCDSi | CCDS36723.1 [Q69Z28-1] |
RefSeqi | NP_742050.2, NM_172053.3 [Q69Z28-1] |
Genome annotation databases
Ensembli | ENSMUST00000080145; ENSMUSP00000079041; ENSMUSG00000049538 [Q69Z28-1] ENSMUST00000109694; ENSMUSP00000105316; ENSMUSG00000049538 [Q69Z28-2] |
GeneIDi | 271127 |
KEGGi | mmu:271127 |
UCSCi | uc007rcz.1, mouse [Q69Z28-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK173338 mRNA Translation: BAD32616.1 BC034739 mRNA Translation: AAH34739.1 |
CCDSi | CCDS36723.1 [Q69Z28-1] |
RefSeqi | NP_742050.2, NM_172053.3 [Q69Z28-1] |
3D structure databases
SMRi | Q69Z28 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 234828, 1 interactor |
STRINGi | 10090.ENSMUSP00000079041 |
Protein family/group databases
MEROPSi | M12.026 |
PTM databases
GlyGeni | Q69Z28, 9 sites |
iPTMneti | Q69Z28 |
PhosphoSitePlusi | Q69Z28 |
Proteomic databases
PaxDbi | Q69Z28 |
PRIDEi | Q69Z28 |
Protocols and materials databases
Antibodypediai | 59056, 38 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000080145; ENSMUSP00000079041; ENSMUSG00000049538 [Q69Z28-1] ENSMUST00000109694; ENSMUSP00000105316; ENSMUSG00000049538 [Q69Z28-2] |
GeneIDi | 271127 |
KEGGi | mmu:271127 |
UCSCi | uc007rcz.1, mouse [Q69Z28-1] |
Organism-specific databases
CTDi | 170690 |
MGIi | MGI:2429637, Adamts16 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG3538, Eukaryota |
GeneTreei | ENSGT00940000159433 |
HOGENOMi | CLU_000660_1_0_1 |
InParanoidi | Q69Z28 |
OMAi | DFHMDLK |
OrthoDBi | 125522at2759 |
PhylomeDBi | Q69Z28 |
TreeFami | TF313537 |
Enzyme and pathway databases
Reactomei | R-MMU-5173214, O-glycosylation of TSR domain-containing proteins |
Miscellaneous databases
BioGRID-ORCSi | 271127, 0 hits in 17 CRISPR screens |
PROi | PR:Q69Z28 |
RNActi | Q69Z28, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000049538, Expressed in efferent duct and 134 other tissues |
Genevisiblei | Q69Z28, MM |
Family and domain databases
Gene3Di | 2.20.100.10, 5 hits 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR041645, ADAM_CR_2 IPR010294, ADAM_spacer1 IPR013273, ADAMTS/ADAMTS-like IPR024079, MetalloPept_cat_dom_sf IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR010909, PLAC IPR000884, TSP1_rpt IPR036383, TSP1_rpt_sf |
Pfami | View protein in Pfam PF17771, ADAM_CR_2, 1 hit PF05986, ADAM_spacer1, 1 hit PF01562, Pep_M12B_propep, 1 hit PF08686, PLAC, 1 hit PF01421, Reprolysin, 1 hit PF00090, TSP_1, 1 hit |
PRINTSi | PR01857, ADAMTSFAMILY |
SMARTi | View protein in SMART SM00209, TSP1, 6 hits |
SUPFAMi | SSF82895, SSF82895, 5 hits |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50900, PLAC, 1 hit PS50092, TSP1, 5 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATS16_MOUSE | |
Accessioni | Q69Z28Primary (citable) accession number: Q69Z28 Secondary accession number(s): Q8K206 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2005 |
Last sequence update: | August 16, 2005 | |
Last modified: | December 2, 2020 | |
This is version 123 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot