UniProtKB - Q68FS4 (AMPL_RAT)
Cytosol aminopeptidase
Lap3
Functioni
Cytosolic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides (PubMed:6108111, PubMed:12675513).
The presence of Zn2+ ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme (By similarity).
For instance, in the presence of Mn2+, it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates (By similarity).
Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status (PubMed:12675513).
By similarity2 PublicationsCatalytic activityi
- EC:3.4.13.231 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.1 Publication EC:3.4.11.1
- Release of N-terminal proline from a peptide.By similarity EC:3.4.11.5
Cofactori
Activity regulationi
Kineticsi
- Vmax=1.6 µmol/min/mg enzyme for peptidase activity with S-benzyl-cysteine-p-nitroanilide1 Publication
- Vmax=11.9 µmol/min/mg enzyme for peptidase activity with Leucine-p-nitroanilide1 Publication
- Vmax=9.4 µmol/min/mg enzyme for peptidase activity with Alanine-p-nitroanilide1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 202 | Zinc 3; via carbonyl oxygen; structuralBy similarity | 1 | |
Metal bindingi | 203 | Zinc 3; via carbonyl oxygen; structuralBy similarity | 1 | |
Metal bindingi | 282 | Zinc 2; catalyticBy similarity | 1 | |
Binding sitei | 282 | SubstrateBy similarity | 1 | |
Metal bindingi | 287 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 287 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 287 | Zinc 2; catalyticBy similarity | 1 | |
Binding sitei | 287 | SubstrateBy similarity | 1 | |
Binding sitei | 292 | SubstrateBy similarity | 1 | |
Active sitei | 294 | By similarity | 1 | |
Binding sitei | 294 | SubstrateBy similarity | 1 | |
Metal bindingi | 303 | Zinc 3; via carbonyl oxygen; structuralBy similarity | 1 | |
Metal bindingi | 305 | Zinc 2; catalyticBy similarity | 1 | |
Binding sitei | 305 | SubstrateBy similarity | 1 | |
Metal bindingi | 364 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 364 | Zinc 1; catalyticBy similarity | 1 | |
Binding sitei | 364 | SubstrateBy similarity | 1 | |
Metal bindingi | 366 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 366 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 366 | Zinc 2; catalyticBy similarity | 1 | |
Active sitei | 368 | By similarity | 1 |
GO - Molecular functioni
- carboxypeptidase activity Source: RHEA
- manganese ion binding Source: InterPro
- metalloaminopeptidase activity Source: InterPro
- peptidase activity Source: RGD
Keywordsi
Molecular function | Aminopeptidase, Hydrolase, Protease |
Ligand | Magnesium, Manganese, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.13.23, 5301 |
Protein family/group databases
MEROPSi | M17.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosol aminopeptidaseCurated (EC:3.4.11.11 Publication)Alternative name(s): Cysteinylglycine-S-conjugate dipeptidase1 Publication (EC:3.4.13.231 Publication) Leucine aminopeptidase 3Imported Short name: LAP-3 Leucyl aminopeptidase1 Publication Short name: LAP1 Publication Peptidase SBy similarity Proline aminopeptidaseBy similarity (EC:3.4.11.5By similarity) Prolyl aminopeptidaseImported |
Gene namesi | Name:Lap3Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1307985, Lap3 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: Ensembl
Golgi apparatus
- trans-Golgi network Source: MGI
Nucleus
- nucleoplasm Source: Ensembl
Other locations
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000274146 | 1 – 519 | Cytosol aminopeptidaseAdd BLAST | 519 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 42 | PhosphoserineCombined sources | 1 | |
Modified residuei | 45 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 54 | PhosphoserineCombined sources | 1 | |
Modified residuei | 61 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 103 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 180 | PhosphoserineBy similarity | 1 | |
Modified residuei | 194 | PhosphoserineBy similarity | 1 | |
Modified residuei | 221 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 221 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 238 | PhosphoserineBy similarity | 1 | |
Modified residuei | 455 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 455 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 476 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 489 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 489 | N6-succinyllysine; alternateBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q68FS4 |
PaxDbi | Q68FS4 |
PRIDEi | Q68FS4 |
PTM databases
iPTMneti | Q68FS4 |
PhosphoSitePlusi | Q68FS4 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000003289, Expressed in jejunum and 21 other tissues |
Genevisiblei | Q68FS4, RN |
Interactioni
Subunit structurei
Homohexamer.
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000004770 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2597, Eukaryota |
GeneTreei | ENSGT00530000063255 |
HOGENOMi | CLU_013734_1_2_1 |
InParanoidi | Q68FS4 |
OMAi | MKNTGPR |
OrthoDBi | 562530at2759 |
PhylomeDBi | Q68FS4 |
TreeFami | TF314954 |
Family and domain databases
CDDi | cd00433, Peptidase_M17, 1 hit |
Gene3Di | 3.40.220.10, 1 hit |
HAMAPi | MF_00181, Cytosol_peptidase_M17, 1 hit |
InterProi | View protein in InterPro IPR011356, Leucine_aapep/pepB IPR043472, Macro_dom-like IPR000819, Peptidase_M17_C IPR023042, Peptidase_M17_leu_NH2_pept IPR008283, Peptidase_M17_N |
PANTHERi | PTHR11963, PTHR11963, 1 hit |
Pfami | View protein in Pfam PF00883, Peptidase_M17, 1 hit PF02789, Peptidase_M17_N, 1 hit |
PRINTSi | PR00481, LAMNOPPTDASE |
SUPFAMi | SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS00631, CYTOSOL_AP, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MYLLPLPAAA RVALRRLGVR GLWDRGLSTA DMTKGLVLGI YSKDKDDDVP
60 70 80 90 100
QFTSAGENFN KLVSGRLREM LNISGPPLKA GKTRTFYGLH QDFPSVVVVG
110 120 130 140 150
LGKRSAGVDD QENWHEGKEN IRAAVAAGCR QVQDLELPSV EVDPCGDAQA
160 170 180 190 200
AAEGAVLGLY EYDDLKQKKK VAVSAKLHGS GDLEAWEKGV LFASGQNLAR
210 220 230 240 250
QLMESPANEM TPTRFAEVIE KNLKSASSKT EVHIRTKSWI EEQEMGSFLS
260 270 280 290 300
VAKGSEEPPV FLEIHYTGSP NATEAPLVFV GKGITFDSGG ISIKASANMD
310 320 330 340 350
LMRADMGGAA TICSAIVSAA KLNLPINIIG LAPLCENMPS GKANKPGDVV
360 370 380 390 400
RARNGKTIQV DNTDAEGRLI LADALCYAHT FNPKVIINAA TLTGAMDVAL
410 420 430 440 450
GSGATGVFTN SSWLWNKLFE ASVETGDRVW RMPLFEHYTR QVIDCQLADV
460 470 480 490 500
NNLGKYRSAG ACTAAAFLRE FVTHTKWAHL DIAGVMTNKD EIPYLRKGMS
510
GRPTRTLIEF LLRFSKDSS
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_022633 | 1 – 31 | Missing in isoform 2. CuratedAdd BLAST | 31 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC079381 mRNA Translation: AAH79381.1 |
RefSeqi | NP_001011910.1, NM_001011910.1 [Q68FS4-1] |
Genome annotation databases
Ensembli | ENSRNOT00000004770; ENSRNOP00000004770; ENSRNOG00000003289 [Q68FS4-1] |
GeneIDi | 289668 |
KEGGi | rno:289668 |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC079381 mRNA Translation: AAH79381.1 |
RefSeqi | NP_001011910.1, NM_001011910.1 [Q68FS4-1] |
3D structure databases
SMRi | Q68FS4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000004770 |
Protein family/group databases
MEROPSi | M17.001 |
PTM databases
iPTMneti | Q68FS4 |
PhosphoSitePlusi | Q68FS4 |
Proteomic databases
jPOSTi | Q68FS4 |
PaxDbi | Q68FS4 |
PRIDEi | Q68FS4 |
Genome annotation databases
Ensembli | ENSRNOT00000004770; ENSRNOP00000004770; ENSRNOG00000003289 [Q68FS4-1] |
GeneIDi | 289668 |
KEGGi | rno:289668 |
Organism-specific databases
CTDi | 51056 |
RGDi | 1307985, Lap3 |
Phylogenomic databases
eggNOGi | KOG2597, Eukaryota |
GeneTreei | ENSGT00530000063255 |
HOGENOMi | CLU_013734_1_2_1 |
InParanoidi | Q68FS4 |
OMAi | MKNTGPR |
OrthoDBi | 562530at2759 |
PhylomeDBi | Q68FS4 |
TreeFami | TF314954 |
Enzyme and pathway databases
BRENDAi | 3.4.13.23, 5301 |
Miscellaneous databases
PROi | PR:Q68FS4 |
Gene expression databases
Bgeei | ENSRNOG00000003289, Expressed in jejunum and 21 other tissues |
Genevisiblei | Q68FS4, RN |
Family and domain databases
CDDi | cd00433, Peptidase_M17, 1 hit |
Gene3Di | 3.40.220.10, 1 hit |
HAMAPi | MF_00181, Cytosol_peptidase_M17, 1 hit |
InterProi | View protein in InterPro IPR011356, Leucine_aapep/pepB IPR043472, Macro_dom-like IPR000819, Peptidase_M17_C IPR023042, Peptidase_M17_leu_NH2_pept IPR008283, Peptidase_M17_N |
PANTHERi | PTHR11963, PTHR11963, 1 hit |
Pfami | View protein in Pfam PF00883, Peptidase_M17, 1 hit PF02789, Peptidase_M17_N, 1 hit |
PRINTSi | PR00481, LAMNOPPTDASE |
SUPFAMi | SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS00631, CYTOSOL_AP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AMPL_RAT | |
Accessioni | Q68FS4Primary (citable) accession number: Q68FS4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2007 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 23, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families