UniProtKB - Q68FR9 (EF1D_RAT)
Elongation factor 1-delta
Eef1d
Functioni
EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.
By similarityRegulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
By similarityGO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- guanyl-nucleotide exchange factor activity Source: GO_Central
- translation elongation factor activity Source: UniProtKB-KW
GO - Biological processi
- cellular response to ionizing radiation Source: RGD
- translational elongation Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Elongation factor |
Biological process | Protein biosynthesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-RNO-156842, Eukaryotic Translation Elongation |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor 1-deltaShort name: EF-1-delta |
Gene namesi | Name:Eef1d |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621174, Eef1d |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: RGD
- eukaryotic translation elongation factor 1 complex Source: InterPro
- glutamatergic synapse Source: RGD
- synapse Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000382456 | 2 – 281 | Elongation factor 1-deltaAdd BLAST | 280 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 17 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 37 | PhosphoserineBy similarity | 1 | |
Modified residuei | 44 | PhosphoserineBy similarity | 1 | |
Modified residuei | 60 | PhosphoserineBy similarity | 1 | |
Modified residuei | 86 | PhosphoserineBy similarity | 1 | |
Modified residuei | 106 | PhosphoserineCombined sources | 1 | |
Modified residuei | 107 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 117 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 117 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 119 | PhosphoserineBy similarity | 1 | |
Modified residuei | 129 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 133 | PhosphoserineCombined sources | 1 | |
Modified residuei | 147 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 162 | Phosphoserine; by CK2Combined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | Q68FR9 |
PaxDbi | Q68FR9 |
PRIDEi | Q68FR9 |
PTM databases
iPTMneti | Q68FR9 |
PhosphoSitePlusi | Q68FR9 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000021638, Expressed in testis and 22 other tissues |
Genevisiblei | Q68FR9, RN |
Interactioni
Subunit structurei
EF-1 is composed of 4 subunits: alpha, beta, delta isoform 1, and gamma.
Isoform 2 interacts with HSF1 and NFE2L2 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 256417, 3 interactors |
IntActi | Q68FR9, 2 interactors |
STRINGi | 10116.ENSRNOP00000034828 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 80 – 115 | Leucine-zipperBy similarityAdd BLAST | 36 | |
Regioni | 115 – 172 | DisorderedSequence analysisAdd BLAST | 58 | |
Regioni | 173 – 281 | Catalytic (GEF)By similarityAdd BLAST | 109 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 115 – 135 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 151 – 165 | Acidic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1668, Eukaryota |
GeneTreei | ENSGT00950000183014 |
HOGENOMi | CLU_020166_0_0_1 |
InParanoidi | Q68FR9 |
OMAi | RVWLDKP |
OrthoDBi | 1464823at2759 |
PhylomeDBi | Q68FR9 |
Family and domain databases
CDDi | cd00292, EF1B, 1 hit |
Gene3Di | 3.30.70.60, 1 hit |
InterProi | View protein in InterPro IPR036219, eEF-1beta-like_sf IPR018940, EF-1_beta_acid_region_euk IPR014038, EF1B_bsu/dsu_GNE IPR014717, Transl_elong_EF1B/ribosomal_S6 IPR001326, Transl_elong_EF1B_B/D_CS |
Pfami | View protein in Pfam PF10587, EF-1_beta_acid, 1 hit PF00736, EF1_GNE, 1 hit |
SMARTi | View protein in SMART SM01182, EF-1_beta_acid, 1 hit SM00888, EF1_GNE, 1 hit |
SUPFAMi | SSF54984, SSF54984, 1 hit |
PROSITEi | View protein in PROSITE PS00825, EF1BD_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATNFLMHEK IWFDKFKYDD AERRFYEQMN GPVTAGSRQE NGASVILRDI
60 70 80 90 100
ARARENIQKS LAGSSGPGAS SGPGGDHSDL IVRIASLEVE NQNLRGVVQD
110 120 130 140 150
LQQAISKLEV RLSTLEKSSP THRATAPQTQ HVSPMRQVEP PAKKGATPAE
160 170 180 190 200
DDEDNDIDLF GSDEEEEDKE AARLREERLR QYAEKKAKKP TLVAKSSILL
210 220 230 240 250
DVKPWDDETD MAQLETCVRS IQLDGLVWGA SKLVPVGYGI RKLQIQCVVE
260 270 280
DDKVGTDLLE EEITKFEEHV QSVDIAAFNK I
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037887 | 1 | M → MRSGKASCALETVWEDKHKY EEAERRFHEHEATQAAAASV QQLLAEVPAVNGPSSQEDAE DTDEAETPNTSSRSDPRKSH ECKKPLQKKRKRSPKSWLGQ ADLALVGLSADHVWLDKPLF DQAESSYRQRLADVAAQAAQ SPALAPRGPCTHGSHVACHH VTWGIWVNKSCFDQAERAFV EWSQALLLAAEGSHREGTPD TGQQAVTPDLALACQPCPPA NGQPPLGSLQALVREVWLEK PRYDAAERGFYEALFDGHPP GKVRLQERASQAEGTRRGRR DRRSRNTVGNKRAGSKRADG EAPSALPYWYFLHKDAEAPW LSKPTYDSAECRHHAAEALR IAWRLEAASLAHRPTPRSGP SMSSLRPKKM in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH473950 Genomic DNA Translation: EDM16037.1 BC079391 mRNA Translation: AAH79391.1 |
RefSeqi | NP_001013122.1, NM_001013104.1 [Q68FR9-2] XP_008763779.1, XM_008765557.1 [Q68FR9-1] XP_008763780.1, XM_008765558.1 [Q68FR9-1] XP_008763781.1, XM_008765559.1 [Q68FR9-1] |
Genome annotation databases
GeneIDi | 300033 |
KEGGi | rno:300033 |
UCSCi | RGD:621174, rat [Q68FR9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH473950 Genomic DNA Translation: EDM16037.1 BC079391 mRNA Translation: AAH79391.1 |
RefSeqi | NP_001013122.1, NM_001013104.1 [Q68FR9-2] XP_008763779.1, XM_008765557.1 [Q68FR9-1] XP_008763780.1, XM_008765558.1 [Q68FR9-1] XP_008763781.1, XM_008765559.1 [Q68FR9-1] |
3D structure databases
AlphaFoldDBi | Q68FR9 |
SMRi | Q68FR9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 256417, 3 interactors |
IntActi | Q68FR9, 2 interactors |
STRINGi | 10116.ENSRNOP00000034828 |
PTM databases
iPTMneti | Q68FR9 |
PhosphoSitePlusi | Q68FR9 |
Proteomic databases
jPOSTi | Q68FR9 |
PaxDbi | Q68FR9 |
PRIDEi | Q68FR9 |
Genome annotation databases
GeneIDi | 300033 |
KEGGi | rno:300033 |
UCSCi | RGD:621174, rat [Q68FR9-1] |
Organism-specific databases
CTDi | 1936 |
RGDi | 621174, Eef1d |
Phylogenomic databases
eggNOGi | KOG1668, Eukaryota |
GeneTreei | ENSGT00950000183014 |
HOGENOMi | CLU_020166_0_0_1 |
InParanoidi | Q68FR9 |
OMAi | RVWLDKP |
OrthoDBi | 1464823at2759 |
PhylomeDBi | Q68FR9 |
Enzyme and pathway databases
Reactomei | R-RNO-156842, Eukaryotic Translation Elongation |
Miscellaneous databases
PROi | PR:Q68FR9 |
Gene expression databases
Bgeei | ENSRNOG00000021638, Expressed in testis and 22 other tissues |
Genevisiblei | Q68FR9, RN |
Family and domain databases
CDDi | cd00292, EF1B, 1 hit |
Gene3Di | 3.30.70.60, 1 hit |
InterProi | View protein in InterPro IPR036219, eEF-1beta-like_sf IPR018940, EF-1_beta_acid_region_euk IPR014038, EF1B_bsu/dsu_GNE IPR014717, Transl_elong_EF1B/ribosomal_S6 IPR001326, Transl_elong_EF1B_B/D_CS |
Pfami | View protein in Pfam PF10587, EF-1_beta_acid, 1 hit PF00736, EF1_GNE, 1 hit |
SMARTi | View protein in SMART SM01182, EF-1_beta_acid, 1 hit SM00888, EF1_GNE, 1 hit |
SUPFAMi | SSF54984, SSF54984, 1 hit |
PROSITEi | View protein in PROSITE PS00825, EF1BD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EF1D_RAT | |
Accessioni | Q68FR9Primary (citable) accession number: Q68FR9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 1, 2009 |
Last sequence update: | September 1, 2009 | |
Last modified: | May 25, 2022 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families