UniProtKB - Q68FP1 (GELS_RAT)
Protein
Gelsolin
Gene
Gsn
Organism
Rattus norvegicus (Rat)
Status
Functioni
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 469 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 470 | Calcium 1By similarity | 1 | |
Metal bindingi | 500 | Calcium 1By similarity | 1 | |
Metal bindingi | 549 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 589 | Calcium 2By similarity | 1 | |
Metal bindingi | 589 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 590 | Calcium 2By similarity | 1 | |
Metal bindingi | 612 | Calcium 2By similarity | 1 | |
Metal bindingi | 694 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 695 | Calcium 3By similarity | 1 | |
Metal bindingi | 717 | Calcium 3By similarity | 1 |
GO - Molecular functioni
- actin binding Source: RGD
- actin filament binding Source: GO_Central
- calcium ion binding Source: RGD
- myosin II binding Source: RGD
- phosphatidylinositol-4,5-bisphosphate binding Source: GO_Central
GO - Biological processi
- actin filament capping Source: RGD
- actin filament polymerization Source: UniProtKB
- actin filament reorganization Source: RGD
- actin filament severing Source: RGD
- actin nucleation Source: InterPro
- actin polymerization or depolymerization Source: RGD
- aging Source: RGD
- amyloid fibril formation Source: RGD
- apoptotic process Source: RGD
- barbed-end actin filament capping Source: GO_Central
- cell projection assembly Source: GO_Central
- cellular response to cadmium ion Source: RGD
- cellular response to interferon-gamma Source: RGD
- central nervous system development Source: GO_Central
- cilium assembly Source: UniProtKB
- hepatocyte apoptotic process Source: RGD
- negative regulation of viral entry into host cell Source: RGD
- oligodendrocyte development Source: RGD
- phagocytosis, engulfment Source: RGD
- phosphatidylinositol-mediated signaling Source: RGD
- positive regulation of actin nucleation Source: RGD
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of keratinocyte apoptotic process Source: RGD
- positive regulation of protein processing in phagocytic vesicle Source: RGD
- protein destabilization Source: RGD
- regulation of cell adhesion Source: RGD
- regulation of establishment of T cell polarity Source: RGD
- regulation of plasma membrane raft polarization Source: RGD
- regulation of podosome assembly Source: RGD
- regulation of receptor clustering Source: RGD
- renal protein absorption Source: RGD
- response to ethanol Source: RGD
- response to folic acid Source: RGD
- sequestering of actin monomers Source: RGD
- striated muscle atrophy Source: RGD
- tissue regeneration Source: RGD
- vesicle-mediated transport Source: RGD
- wound healing Source: RGD
Keywordsi
Molecular function | Actin capping, Actin-binding |
Biological process | Cilium biogenesis/degradation |
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: GelsolinAlternative name(s): Actin-depolymerizing factor Short name: ADF Brevin |
Gene namesi | Name:Gsn |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1303089, Gsn |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Cytoskeleton
- cytoskeleton By similarity
Note: A cytoplasmic form may also exist.By similarity
Cytoskeleton
- actin cap Source: RGD
- actin cytoskeleton Source: RGD
- cortical actin cytoskeleton Source: RGD
- podosome Source: RGD
Cytosol
- cytosol Source: UniProtKB
Extracellular region or secreted
- extracellular region Source: UniProtKB
- extracellular space Source: RGD
Nucleus
- nucleus Source: Ensembl
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- cytoplasm Source: RGD
- focal adhesion Source: Ensembl
- lamellipodium Source: RGD
- myelin sheath Source: UniProtKB
- perinuclear region of cytoplasm Source: RGD
- phagocytic vesicle Source: RGD
- protein-containing complex Source: RGD
- ruffle Source: RGD
- sarcoplasm Source: RGD
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_0000288477 | 26 – 780 | GelsolinAdd BLAST | 755 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 84 | PhosphotyrosineBy similarity | 1 | |
Disulfide bondi | 213 ↔ 226 | By similarity | ||
Modified residuei | 407 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 463 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 582 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 601 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 649 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 740 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Phosphorylated on tyrosine residues in vitro.By similarity
Keywords - PTMi
Acetylation, Disulfide bond, PhosphoproteinProteomic databases
jPOSTi | Q68FP1 |
PaxDbi | Q68FP1 |
PeptideAtlasi | Q68FP1 |
PRIDEi | Q68FP1 |
PTM databases
iPTMneti | Q68FP1 |
PhosphoSitePlusi | Q68FP1 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000018991, Expressed in lung and 22 other tissues |
Genevisiblei | Q68FP1, RN |
Interactioni
Subunit structurei
Binds to actin and to fibronectin.
Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X (By similarity).
Interacts with the inactive form of EIF2AK2/PKR (By similarity).
By similarityGO - Molecular functioni
- actin binding Source: RGD
- actin filament binding Source: GO_Central
- myosin II binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 255439, 5 interactors |
CORUMi | Q68FP1 |
IntActi | Q68FP1, 6 interactors |
STRINGi | 10116.ENSRNOP00000025857 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 74 – 124 | Gelsolin-like 1Add BLAST | 51 | |
Repeati | 196 – 236 | Gelsolin-like 2Add BLAST | 41 | |
Repeati | 312 – 354 | Gelsolin-like 3Add BLAST | 43 | |
Repeati | 451 – 502 | Gelsolin-like 4Add BLAST | 52 | |
Repeati | 574 – 614 | Gelsolin-like 5Add BLAST | 41 | |
Repeati | 677 – 719 | Gelsolin-like 6Add BLAST | 43 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 51 – 174 | Actin-severingSequence analysisAdd BLAST | 124 | |
Regioni | 121 – 124 | Actin-actin interfilament contact pointBy similarity | 4 | |
Regioni | 160 – 167 | Polyphosphoinositide bindingBy similarity | 8 | |
Regioni | 186 – 194 | Polyphosphoinositide bindingBy similarity | 9 | |
Regioni | 432 – 780 | Actin-binding, Ca-sensitiveSequence analysisAdd BLAST | 349 |
Sequence similaritiesi
Belongs to the villin/gelsolin family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG0443, Eukaryota |
GeneTreei | ENSGT00940000155591 |
InParanoidi | Q68FP1 |
OMAi | EWPQTPD |
OrthoDBi | 1376537at2759 |
PhylomeDBi | Q68FP1 |
Family and domain databases
Gene3Di | 3.40.20.10, 6 hits |
InterProi | View protein in InterPro IPR029006, ADF-H/Gelsolin-like_dom_sf IPR030004, Gelsolin IPR007123, Gelsolin-like_dom IPR007122, Villin/Gelsolin |
PANTHERi | PTHR11977, PTHR11977, 1 hit PTHR11977:SF29, PTHR11977:SF29, 1 hit |
Pfami | View protein in Pfam PF00626, Gelsolin, 6 hits |
PRINTSi | PR00597, GELSOLIN |
SMARTi | View protein in SMART SM00262, GEL, 6 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketIsoform 1 (identifier: Q68FP1-1) [UniParc]FASTAAdd to basket
Also known as: Secreted, Plasma
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPYCSSLRS ALLVLALCAL SPSHAATASR GRAQERAPQS RVSETRPSTM
60 70 80 90 100
VVEHPEFLKA GKEPGLQIWR VEKFDLVPVP PNLYGDFFTG DAYVILKTVQ
110 120 130 140 150
LRNGNLQYDL HYWLGNECSQ DESGAAAIFT VQLDDYLNGR AVQHREVQGF
160 170 180 190 200
ESSTFQGYFK SGLKYKKGGV ASGFKHVVPN EVVVQRLFQV KGRRVVRATE
210 220 230 240 250
VPVSWDSFNN GDCFILDLGN NIYQWCGSGS NKFERLKATQ VSKGIRDNER
260 270 280 290 300
SGRAQVHVSE EGSEPEAMLQ VLGPKPDLPQ GTEDTAKEDA ANRRLAKLYK
310 320 330 340 350
VSNSGGSMSV SLVADENPFA QSALRSEDCF ILDHGRDGKI FVWKGKQANM
360 370 380 390 400
DERKAALKTA SDFISKMQYP RQTQVSVLPE GGETPLFKQF FKNWRDPDQT
410 420 430 440 450
DGPGLSYLSS HIANVERVPF DAATLHTSTA MAAQHGMDDD GTGQKQIWRI
460 470 480 490 500
EGSNKVLVDP ATYGQFYGGD SYIILYNYRH GGRQGQIIYN WQGAQSTQDE
510 520 530 540 550
VAASAILTAQ LDEELGGTPV QSRVVQGKEP AHLMSLFGGK PMIIYKGGTS
560 570 580 590 600
RDGGQTTPAS TRLFQVRASS SGATRAVEVM PKAGALNSND AFVLKTPSAA
610 620 630 640 650
YLWVGTGASD AEKTGALELL KVLRAQHVQV EEGSEPDGFW EALGGKTAYR
660 670 680 690 700
TSPRLKDKKM DAHPPRLFAC SNRIGRFVIE EVPGELMQED LATDDVMLLD
710 720 730 740 750
TWDQVFVWVG KDSQEEEKTE ALTSAKRYIE TDPANRDRRT PITVVRQGFE
760 770 780
PPSFVGWFLG WDDDYWSVDP LDRALAELAA
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_036722 | 1 – 49 | Missing in isoform 2. CuratedAdd BLAST | 49 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC079472 mRNA Translation: AAH79472.1 |
RefSeqi | NP_001004080.1, NM_001004080.1 [Q68FP1-1] XP_006234102.1, XM_006234040.2 [Q68FP1-2] |
Genome annotation databases
Ensembli | ENSRNOT00000025857; ENSRNOP00000025857; ENSRNOG00000018991 [Q68FP1-1] ENSRNOT00000078939; ENSRNOP00000075631; ENSRNOG00000018991 [Q68FP1-2] |
GeneIDi | 296654 |
KEGGi | rno:296654 |
UCSCi | RGD:1303089, rat [Q68FP1-1] |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC079472 mRNA Translation: AAH79472.1 |
RefSeqi | NP_001004080.1, NM_001004080.1 [Q68FP1-1] XP_006234102.1, XM_006234040.2 [Q68FP1-2] |
3D structure databases
SMRi | Q68FP1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 255439, 5 interactors |
CORUMi | Q68FP1 |
IntActi | Q68FP1, 6 interactors |
STRINGi | 10116.ENSRNOP00000025857 |
PTM databases
iPTMneti | Q68FP1 |
PhosphoSitePlusi | Q68FP1 |
Proteomic databases
jPOSTi | Q68FP1 |
PaxDbi | Q68FP1 |
PeptideAtlasi | Q68FP1 |
PRIDEi | Q68FP1 |
Genome annotation databases
Ensembli | ENSRNOT00000025857; ENSRNOP00000025857; ENSRNOG00000018991 [Q68FP1-1] ENSRNOT00000078939; ENSRNOP00000075631; ENSRNOG00000018991 [Q68FP1-2] |
GeneIDi | 296654 |
KEGGi | rno:296654 |
UCSCi | RGD:1303089, rat [Q68FP1-1] |
Organism-specific databases
CTDi | 2934 |
RGDi | 1303089, Gsn |
Phylogenomic databases
eggNOGi | KOG0443, Eukaryota |
GeneTreei | ENSGT00940000155591 |
InParanoidi | Q68FP1 |
OMAi | EWPQTPD |
OrthoDBi | 1376537at2759 |
PhylomeDBi | Q68FP1 |
Miscellaneous databases
PROi | PR:Q68FP1 |
Gene expression databases
Bgeei | ENSRNOG00000018991, Expressed in lung and 22 other tissues |
Genevisiblei | Q68FP1, RN |
Family and domain databases
Gene3Di | 3.40.20.10, 6 hits |
InterProi | View protein in InterPro IPR029006, ADF-H/Gelsolin-like_dom_sf IPR030004, Gelsolin IPR007123, Gelsolin-like_dom IPR007122, Villin/Gelsolin |
PANTHERi | PTHR11977, PTHR11977, 1 hit PTHR11977:SF29, PTHR11977:SF29, 1 hit |
Pfami | View protein in Pfam PF00626, Gelsolin, 6 hits |
PRINTSi | PR00597, GELSOLIN |
SMARTi | View protein in SMART SM00262, GEL, 6 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GELS_RAT | |
Accessioni | Q68FP1Primary (citable) accession number: Q68FP1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2007 |
Last sequence update: | October 11, 2004 | |
Last modified: | December 2, 2020 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families