UniProtKB - Q68FF6 (GIT1_MOUSE)
ARF GTPase-activating protein GIT1
Git1
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 11 – 34 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- GTPase activator activity Source: MGI
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- protein-containing complex binding Source: MGI
- small GTPase binding Source: MGI
- structural constituent of postsynaptic specialization Source: MGI
GO - Biological processi
- cellular response to epidermal growth factor stimulus Source: MGI
- dendritic spine development Source: MGI
- ephrin receptor signaling pathway Source: MGI
- immunological synapse formation Source: MGI
- negative regulation of ARF protein signal transduction Source: MGI
- neurotransmitter receptor localization to postsynaptic specialization membrane Source: MGI
- positive regulation of receptor catabolic process Source: MGI
- presynaptic modulation of chemical synaptic transmission Source: SynGO
- regulation of cytokinesis Source: MGI
- regulation of G protein-coupled receptor signaling pathway Source: MGI
- regulation of synaptic vesicle exocytosis Source: SynGO
Keywordsi
Molecular function | GTPase activation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-3928664, Ephrin signaling |
Names & Taxonomyi
Protein namesi | Recommended name: ARF GTPase-activating protein GIT1Short name: ARF GAP GIT1 Alternative name(s): G protein-coupled receptor kinase-interactor 1 GRK-interacting protein 1 |
Gene namesi | Name:Git1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1927140, Git1 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
Note: Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin (By similarity).By similarity
Cytosol
- cytosol Source: MGI
Endosome
- endosome Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Other locations
- calyx of Held Source: SynGO
- focal adhesion Source: MGI
- GABA-ergic synapse Source: MGI
- neuron projection Source: MGI
- synapse Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074201 | 1 – 770 | ARF GTPase-activating protein GIT1Add BLAST | 770 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 224 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 368 | PhosphoserineCombined sources | 1 | |
Modified residuei | 371 | PhosphoserineCombined sources | 1 | |
Modified residuei | 373 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 379 | PhosphoserineBy similarity | 1 | |
Modified residuei | 384 | PhosphoserineBy similarity | 1 | |
Modified residuei | 392 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 394 | PhosphoserineCombined sources | 1 | |
Modified residuei | 397 | PhosphoserineCombined sources | 1 | |
Modified residuei | 401 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 419 | PhosphoserineCombined sources | 1 | |
Modified residuei | 422 | PhosphoserineCombined sources | 1 | |
Modified residuei | 426 | PhosphoserineCombined sources | 1 | |
Modified residuei | 507 | PhosphoserineBy similarity | 1 | |
Modified residuei | 545 | PhosphoserineCombined sources | 1 | |
Modified residuei | 546 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 554 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 563 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 570 | PhosphoserineCombined sources | 1 | |
Modified residuei | 580 | PhosphoserineBy similarity | 1 | |
Modified residuei | 601 | PhosphoserineCombined sources | 1 | |
Modified residuei | 605 | PhosphoserineCombined sources | 1 | |
Modified residuei | 610 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 639 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q68FF6 |
jPOSTi | Q68FF6 |
MaxQBi | Q68FF6 |
PaxDbi | Q68FF6 |
PeptideAtlasi | Q68FF6 |
PRIDEi | Q68FF6 |
PTM databases
iPTMneti | Q68FF6 |
PhosphoSitePlusi | Q68FF6 |
SwissPalmi | Q68FF6 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000011877, Expressed in primary visual cortex and 287 other tissues |
ExpressionAtlasi | Q68FF6, baseline and differential |
Genevisiblei | Q68FF6, MM |
Interactioni
Subunit structurei
Interacts with G protein-coupled receptor kinases: GRK2, PPFIA1 and PPFIA4, with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1.
Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1.
Interacts with SCRIB (By similarity).
Interacts with TGFB1I1.
Interacts (via N- and C-terminus) with ENTR1 (via N-terminus); this interaction is direct (By similarity).
Found in a complex with ENTR1, PTPN13 and GIT1 (By similarity).
Interacts with YWHAZ (PubMed:16959763).
By similarity2 PublicationsBinary interactionsi
Q68FF6
With | #Exp. | IntAct |
---|---|---|
Arhgef7 [Q9ES28] | 2 | EBI-645933,EBI-642580 |
GO - Molecular functioni
- identical protein binding Source: MGI
- small GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 229822, 29 interactors |
CORUMi | Q68FF6 |
IntActi | Q68FF6, 32 interactors |
MINTi | Q68FF6 |
STRINGi | 10090.ENSMUSP00000037210 |
Miscellaneous databases
RNActi | Q68FF6, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q68FF6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 124 | Arf-GAPPROSITE-ProRule annotationAdd BLAST | 124 | |
Repeati | 132 – 161 | ANK 1Add BLAST | 30 | |
Repeati | 166 – 195 | ANK 2Add BLAST | 30 | |
Repeati | 199 – 228 | ANK 3Add BLAST | 30 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 253 – 424 | PTK2/FAK1-bindingBy similarityAdd BLAST | 172 | |
Regioni | 254 – 376 | ARHGEF6-bindingBy similarityAdd BLAST | 123 | |
Regioni | 646 – 770 | Interaction with PXN and TGFB1I1By similarityAdd BLAST | 125 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 11 – 34 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
ANK repeat, Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0818, Eukaryota |
GeneTreei | ENSGT00940000159604 |
InParanoidi | Q68FF6 |
OMAi | CSEKIHA |
OrthoDBi | 349344at2759 |
PhylomeDBi | Q68FF6 |
TreeFami | TF317762 |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf IPR032352, GIT1/2_CC IPR022018, GIT1_C IPR013724, GIT_SHD |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF01412, ArfGap, 1 hit PF12205, GIT1_C, 1 hit PF16559, GIT_CC, 1 hit PF08518, GIT_SHD, 2 hits |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00248, ANK, 3 hits SM00105, ArfGap, 1 hit SM00555, GIT, 2 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 1 hit PS50115, ARFGAP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSRKGPRAEV CADCSAPDPG WASISRGVLV CDECCSVHRS LGRHISIVKH
60 70 80 90 100
LRHSAWPPTL LQMVHTLASN GANSIWEHSL LDPAQVQSGR RKANPQDKVH
110 120 130 140 150
PIKSEFIRAK YQMLAFVHKL PCRDDDGVTA KDLSKQLHSS VRTGNLETCL
160 170 180 190 200
RLLSLGAQAN FFHPEKGTTP LHVAAKAGQT LQAELLVVYG ADPGSPDVNG
210 220 230 240 250
RTPIDYARQA GHHELAERLV ECQYELTDRL AFYLCGRKPD HKNGHYIIPQ
260 270 280 290 300
MADRSRQKCM SQSLDLSELA KAAKKKLQAL SNRLFEELAM DVYDEVDRRE
310 320 330 340 350
NDAVWLATQN HSTLVTERSA VPFLPVNPEY SATRNQGRQK LARFNAREFA
360 370 380 390 400
TLIIDILSEA KRRQQGKSLS SPTDNLELSA RSQSELDDQH DYDSVASDED
410 420 430 440 450
TDQEPLPSAG ATRNNRARSM DSSDLSDGAV TLQEYLELKK ALATSEAKVQ
460 470 480 490 500
QLMKVNSSLS DELRRLQREI HKLQAENLQL RQPPGPVPPP SLPSERAEHT
510 520 530 540 550
LMGPGGSTHR RDRQAFSMYE PGSALKPFGG TPGDELATRL QPFHSTELED
560 570 580 590 600
DAIYSVHVPA GLYRIRKGVS ASSVPFTPSS PLLSCSQEGS RHASKLSRHG
610 620 630 640 650
SGADSDYENT QSGDPLLGLE GKRFLELSKE DELHPELESL DGDLDPGLPS
660 670 680 690 700
TEDVILKTEQ VTKNIQELLR AAQEFKHDSF VPCSEKIHLA VTEMASLFPK
710 720 730 740 750
RPALEPVRSS LRLLNASAYR LQSECRKTVP PEPGAPVDFQ LLTQQVIQCA
760 770
YDIAKAAKQL VTITTREKKQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5F258 | Q5F258_MOUSE | ARF GTPase-activating protein GIT1 | Git1 | 761 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL607072 Genomic DNA No translation available. BC079870 mRNA Translation: AAH79870.1 |
CCDSi | CCDS25080.1 |
RefSeqi | NP_001004144.1, NM_001004144.1 |
Genome annotation databases
Ensembli | ENSMUST00000037285; ENSMUSP00000037210; ENSMUSG00000011877 |
GeneIDi | 216963 |
UCSCi | uc007kgy.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL607072 Genomic DNA No translation available. BC079870 mRNA Translation: AAH79870.1 |
CCDSi | CCDS25080.1 |
RefSeqi | NP_001004144.1, NM_001004144.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6JMU | X-ray | 2.00 | A/B | 640-770 | [»] | |
SMRi | Q68FF6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 229822, 29 interactors |
CORUMi | Q68FF6 |
IntActi | Q68FF6, 32 interactors |
MINTi | Q68FF6 |
STRINGi | 10090.ENSMUSP00000037210 |
PTM databases
iPTMneti | Q68FF6 |
PhosphoSitePlusi | Q68FF6 |
SwissPalmi | Q68FF6 |
Proteomic databases
EPDi | Q68FF6 |
jPOSTi | Q68FF6 |
MaxQBi | Q68FF6 |
PaxDbi | Q68FF6 |
PeptideAtlasi | Q68FF6 |
PRIDEi | Q68FF6 |
Protocols and materials databases
Antibodypediai | 1365, 474 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000037285; ENSMUSP00000037210; ENSMUSG00000011877 |
GeneIDi | 216963 |
UCSCi | uc007kgy.1, mouse |
Organism-specific databases
CTDi | 28964 |
MGIi | MGI:1927140, Git1 |
Phylogenomic databases
eggNOGi | KOG0818, Eukaryota |
GeneTreei | ENSGT00940000159604 |
InParanoidi | Q68FF6 |
OMAi | CSEKIHA |
OrthoDBi | 349344at2759 |
PhylomeDBi | Q68FF6 |
TreeFami | TF317762 |
Enzyme and pathway databases
Reactomei | R-MMU-3928664, Ephrin signaling |
Miscellaneous databases
BioGRID-ORCSi | 216963, 0 hits in 17 CRISPR screens |
ChiTaRSi | Git1, mouse |
PROi | PR:Q68FF6 |
RNActi | Q68FF6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000011877, Expressed in primary visual cortex and 287 other tissues |
ExpressionAtlasi | Q68FF6, baseline and differential |
Genevisiblei | Q68FF6, MM |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR037278, ARFGAP/RecO IPR001164, ArfGAP_dom IPR038508, ArfGAP_dom_sf IPR032352, GIT1/2_CC IPR022018, GIT1_C IPR013724, GIT_SHD |
Pfami | View protein in Pfam PF12796, Ank_2, 1 hit PF01412, ArfGap, 1 hit PF12205, GIT1_C, 1 hit PF16559, GIT_CC, 1 hit PF08518, GIT_SHD, 2 hits |
PRINTSi | PR00405, REVINTRACTNG |
SMARTi | View protein in SMART SM00248, ANK, 3 hits SM00105, ArfGap, 1 hit SM00555, GIT, 2 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF57863, SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 1 hit PS50115, ARFGAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GIT1_MOUSE | |
Accessioni | Q68FF6Primary (citable) accession number: Q68FF6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 13, 2005 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 10, 2021 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references