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Protein

ARF GTPase-activating protein GIT1

Gene

Git1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes (By similarity). Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (PubMed:23108400).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 34C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3928664 Ephrin signaling

Names & Taxonomyi

Protein namesi
Recommended name:
ARF GTPase-activating protein GIT1
Short name:
ARF GAP GIT1
Alternative name(s):
G protein-coupled receptor kinase-interactor 1
GRK-interacting protein 1
Gene namesi
Name:Git1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1927140 Git1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000742011 – 770ARF GTPase-activating protein GIT1Add BLAST770

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224PhosphotyrosineCombined sources1
Modified residuei368PhosphoserineCombined sources1
Modified residuei371PhosphoserineCombined sources1
Modified residuei373PhosphothreonineBy similarity1
Modified residuei379PhosphoserineBy similarity1
Modified residuei384PhosphoserineBy similarity1
Modified residuei392PhosphotyrosineBy similarity1
Modified residuei394PhosphoserineCombined sources1
Modified residuei397PhosphoserineCombined sources1
Modified residuei401PhosphothreonineCombined sources1
Modified residuei419PhosphoserineCombined sources1
Modified residuei422PhosphoserineCombined sources1
Modified residuei426PhosphoserineCombined sources1
Modified residuei507PhosphoserineBy similarity1
Modified residuei545PhosphoserineCombined sources1
Modified residuei546PhosphothreonineCombined sources1
Modified residuei554PhosphotyrosineCombined sources1
Modified residuei563PhosphotyrosineCombined sources1
Modified residuei570PhosphoserineCombined sources1
Modified residuei580PhosphoserineBy similarity1
Modified residuei601PhosphoserineCombined sources1
Modified residuei605PhosphoserineCombined sources1
Modified residuei610PhosphothreonineCombined sources1
Modified residuei639PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ68FF6
MaxQBiQ68FF6
PaxDbiQ68FF6
PeptideAtlasiQ68FF6
PRIDEiQ68FF6

PTM databases

iPTMnetiQ68FF6
PhosphoSitePlusiQ68FF6

Expressioni

Gene expression databases

BgeeiENSMUSG00000011877 Expressed in 271 organ(s), highest expression level in cardiac ventricle
CleanExiMM_GIT1
ExpressionAtlasiQ68FF6 baseline and differential
GenevisibleiQ68FF6 MM

Interactioni

Subunit structurei

Interacts with G protein-coupled receptor kinases: GRK2, PPFIA1 and PPFIA4, with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with SCRIB (By similarity). Interacts with TGFB1I1. Interacts (via N- and C-terminus) with ENTR1 (via N-terminus); this interaction is direct (By similarity). Found in a complex with ENTR1, PTPN13 and GIT1 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Arhgef7Q9ES282EBI-645933,EBI-642580

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229822, 21 interactors
CORUMiQ68FF6
IntActiQ68FF6, 31 interactors
MINTiQ68FF6
STRINGi10090.ENSMUSP00000037210

Structurei

3D structure databases

ProteinModelPortaliQ68FF6
SMRiQ68FF6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 124Arf-GAPPROSITE-ProRule annotationAdd BLAST124
Repeati132 – 161ANK 1Add BLAST30
Repeati166 – 195ANK 2Add BLAST30
Repeati199 – 228ANK 3Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni253 – 424PTK2/FAK1-bindingBy similarityAdd BLAST172
Regioni254 – 376ARHGEF6-bindingBy similarityAdd BLAST123
Regioni646 – 770Interaction with PXN and TGFB1I1By similarityAdd BLAST125

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 34C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0818 Eukaryota
ENOG410XR8U LUCA
GeneTreeiENSGT00930000150886
HOGENOMiHOG000232135
HOVERGENiHBG012506
InParanoidiQ68FF6
KOiK05737
OMAiQKCMSQS
OrthoDBiEOG091G03VD
PhylomeDBiQ68FF6
TreeFamiTF317762

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
3.30.40.160, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR037278 ARFGAP/RecO
IPR001164 ArfGAP_dom
IPR038508 ArfGAP_dom_sf
IPR032352 GIT1/2_CC
IPR022018 GIT1_C
IPR013724 GIT_SHD
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF01412 ArfGap, 1 hit
PF12205 GIT1_C, 1 hit
PF16559 GIT_CC, 1 hit
PF08518 GIT_SHD, 2 hits
PRINTSiPR00405 REVINTRACTNG
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SM00105 ArfGap, 1 hit
SM00555 GIT, 2 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF57863 SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit
PS50115 ARFGAP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q68FF6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRKGPRAEV CADCSAPDPG WASISRGVLV CDECCSVHRS LGRHISIVKH
60 70 80 90 100
LRHSAWPPTL LQMVHTLASN GANSIWEHSL LDPAQVQSGR RKANPQDKVH
110 120 130 140 150
PIKSEFIRAK YQMLAFVHKL PCRDDDGVTA KDLSKQLHSS VRTGNLETCL
160 170 180 190 200
RLLSLGAQAN FFHPEKGTTP LHVAAKAGQT LQAELLVVYG ADPGSPDVNG
210 220 230 240 250
RTPIDYARQA GHHELAERLV ECQYELTDRL AFYLCGRKPD HKNGHYIIPQ
260 270 280 290 300
MADRSRQKCM SQSLDLSELA KAAKKKLQAL SNRLFEELAM DVYDEVDRRE
310 320 330 340 350
NDAVWLATQN HSTLVTERSA VPFLPVNPEY SATRNQGRQK LARFNAREFA
360 370 380 390 400
TLIIDILSEA KRRQQGKSLS SPTDNLELSA RSQSELDDQH DYDSVASDED
410 420 430 440 450
TDQEPLPSAG ATRNNRARSM DSSDLSDGAV TLQEYLELKK ALATSEAKVQ
460 470 480 490 500
QLMKVNSSLS DELRRLQREI HKLQAENLQL RQPPGPVPPP SLPSERAEHT
510 520 530 540 550
LMGPGGSTHR RDRQAFSMYE PGSALKPFGG TPGDELATRL QPFHSTELED
560 570 580 590 600
DAIYSVHVPA GLYRIRKGVS ASSVPFTPSS PLLSCSQEGS RHASKLSRHG
610 620 630 640 650
SGADSDYENT QSGDPLLGLE GKRFLELSKE DELHPELESL DGDLDPGLPS
660 670 680 690 700
TEDVILKTEQ VTKNIQELLR AAQEFKHDSF VPCSEKIHLA VTEMASLFPK
710 720 730 740 750
RPALEPVRSS LRLLNASAYR LQSECRKTVP PEPGAPVDFQ LLTQQVIQCA
760 770
YDIAKAAKQL VTITTREKKQ
Length:770
Mass (Da):85,300
Last modified:October 11, 2004 - v1
Checksum:iC516E7A49578D0B4
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5F258Q5F258_MOUSE
ARF GTPase-activating protein GIT1
Git1
761Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL607072 Genomic DNA No translation available.
BC079870 mRNA Translation: AAH79870.1
CCDSiCCDS25080.1
RefSeqiNP_001004144.1, NM_001004144.1
UniGeneiMm.290182

Genome annotation databases

EnsembliENSMUST00000037285; ENSMUSP00000037210; ENSMUSG00000011877
GeneIDi216963
KEGGimmu:216963
UCSCiuc007kgy.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL607072 Genomic DNA No translation available.
BC079870 mRNA Translation: AAH79870.1
CCDSiCCDS25080.1
RefSeqiNP_001004144.1, NM_001004144.1
UniGeneiMm.290182

3D structure databases

ProteinModelPortaliQ68FF6
SMRiQ68FF6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229822, 21 interactors
CORUMiQ68FF6
IntActiQ68FF6, 31 interactors
MINTiQ68FF6
STRINGi10090.ENSMUSP00000037210

PTM databases

iPTMnetiQ68FF6
PhosphoSitePlusiQ68FF6

Proteomic databases

EPDiQ68FF6
MaxQBiQ68FF6
PaxDbiQ68FF6
PeptideAtlasiQ68FF6
PRIDEiQ68FF6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037285; ENSMUSP00000037210; ENSMUSG00000011877
GeneIDi216963
KEGGimmu:216963
UCSCiuc007kgy.1 mouse

Organism-specific databases

CTDi28964
MGIiMGI:1927140 Git1

Phylogenomic databases

eggNOGiKOG0818 Eukaryota
ENOG410XR8U LUCA
GeneTreeiENSGT00930000150886
HOGENOMiHOG000232135
HOVERGENiHBG012506
InParanoidiQ68FF6
KOiK05737
OMAiQKCMSQS
OrthoDBiEOG091G03VD
PhylomeDBiQ68FF6
TreeFamiTF317762

Enzyme and pathway databases

ReactomeiR-MMU-3928664 Ephrin signaling

Miscellaneous databases

ChiTaRSiGit1 mouse
PROiPR:Q68FF6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000011877 Expressed in 271 organ(s), highest expression level in cardiac ventricle
CleanExiMM_GIT1
ExpressionAtlasiQ68FF6 baseline and differential
GenevisibleiQ68FF6 MM

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
3.30.40.160, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR037278 ARFGAP/RecO
IPR001164 ArfGAP_dom
IPR038508 ArfGAP_dom_sf
IPR032352 GIT1/2_CC
IPR022018 GIT1_C
IPR013724 GIT_SHD
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF01412 ArfGap, 1 hit
PF12205 GIT1_C, 1 hit
PF16559 GIT_CC, 1 hit
PF08518 GIT_SHD, 2 hits
PRINTSiPR00405 REVINTRACTNG
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SM00105 ArfGap, 1 hit
SM00555 GIT, 2 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF57863 SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit
PS50115 ARFGAP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGIT1_MOUSE
AccessioniPrimary (citable) accession number: Q68FF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: October 11, 2004
Last modified: November 7, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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