Git1

Functionⁱ

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes (By similarity). Involved in the regulation of cytokinesis; the function may involve ENTR1 and PTPN13 (PubMed:23108400).By similarity1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	11 – 34	C4-typePROSITE-ProRule annotationAdd BLAST		24

GO - Molecular functionⁱ

GTPase activator activity Source: MGI
metal ion binding Source: UniProtKB-KW
protein-containing complex binding
Source: MGI
Inferred from physical interactionⁱ
- 17310244
protein homodimerization activity Source: MGI
small GTPase binding Source: MGI
structural constituent of postsynaptic specialization Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to epidermal growth factor stimulus Source: MGI
dendritic spine development
Source: MGI
Inferred from sequence orthologyⁱ
- 17310244
ephrin receptor signaling pathway
Source: MGI
Inferred from direct assayⁱ
- 17310244
immunological synapse formation
Source: MGI
Inferred from sequence orthologyⁱ
- 17310244
negative regulation of ARF protein signal transduction Source: MGI
neurotransmitter receptor localization to postsynaptic specialization membrane Source: MGI
positive regulation of receptor catabolic process Source: MGI
presynaptic modulation of chemical synaptic transmission
Source: SynGO
Inferred from mutant phenotypeⁱ
- 26637799
regulation of cytokinesis Source: MGI
regulation of G protein-coupled receptor signaling pathway Source: MGI
regulation of synaptic vesicle exocytosis
Source: SynGO
Inferred from mutant phenotypeⁱ
- 26637799

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	GTPase activation
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-MMU-3928664 Ephrin signaling

Reactomeⁱ

R-MMU-3928664 Ephrin signaling

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ARF GTPase-activating protein GIT1 Short name: ARF GAP GIT1 Alternative name(s): G protein-coupled receptor kinase-interactor 1 GRK-interacting protein 1
Gene namesⁱ	Name:Git1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 11

Organism-specific databases

MGIⁱ	MGI:1927140 Git1

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000074201	1 – 770	ARF GTPase-activating protein GIT1Add BLAST		770

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	224	PhosphotyrosineCombined sources	1
Modified residueⁱ	368	PhosphoserineCombined sources	1
Modified residueⁱ	371	PhosphoserineCombined sources	1
Modified residueⁱ	373	PhosphothreonineBy similarity	1
Modified residueⁱ	379	PhosphoserineBy similarity	1
Modified residueⁱ	384	PhosphoserineBy similarity	1
Modified residueⁱ	392	PhosphotyrosineBy similarity	1
Modified residueⁱ	394	PhosphoserineCombined sources	1
Modified residueⁱ	397	PhosphoserineCombined sources	1
Modified residueⁱ	401	PhosphothreonineCombined sources	1
Modified residueⁱ	419	PhosphoserineCombined sources	1
Modified residueⁱ	422	PhosphoserineCombined sources	1
Modified residueⁱ	426	PhosphoserineCombined sources	1
Modified residueⁱ	507	PhosphoserineBy similarity	1
Modified residueⁱ	545	PhosphoserineCombined sources	1
Modified residueⁱ	546	PhosphothreonineCombined sources	1
Modified residueⁱ	554	PhosphotyrosineCombined sources	1
Modified residueⁱ	563	PhosphotyrosineCombined sources	1
Modified residueⁱ	570	PhosphoserineCombined sources	1
Modified residueⁱ	580	PhosphoserineBy similarity	1
Modified residueⁱ	601	PhosphoserineCombined sources	1
Modified residueⁱ	605	PhosphoserineCombined sources	1
Modified residueⁱ	610	PhosphothreonineCombined sources	1
Modified residueⁱ	639	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Phosphorylated on tyrosine residues by PTK2/FAK1 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase (By similarity).By similarity

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q68FF6
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q68FF6
PaxDbⁱ	Q68FF6
PeptideAtlas More...PeptideAtlasⁱ	Q68FF6
PRIDEⁱ	Q68FF6

PTM databases

iPTMnetⁱ	Q68FF6
PhosphoSitePlusⁱ	Q68FF6

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000011877 Expressed in 271 organ(s), highest expression level in cardiac ventricle
CleanExⁱ	MM_GIT1
ExpressionAtlasⁱ	Q68FF6 baseline and differential
Genevisibleⁱ	Q68FF6 MM

Interactionⁱ

Subunit structureⁱ

Interacts with G protein-coupled receptor kinases: GRK2, PPFIA1 and PPFIA4, with ARHGEF6/alpha-PIX, with ARHGEF7/beta-PIX, with PXN/paxillin and with PTK2/FAK1. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and PAK1. Interacts with SCRIB (By similarity). Interacts with TGFB1I1. Interacts (via N- and C-terminus) with ENTR1 (via N-terminus); this interaction is direct (By similarity). Found in a complex with ENTR1, PTPN13 and GIT1 (By similarity).By similarity1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Arhgef7	Q9ES28	2	EBI-645933,EBI-642580

With

Entry

#Exp.

IntAct

Notes

Arhgef7

Q9ES28

2

EBI-645933,EBI-642580

GO - Molecular functionⁱ

protein homodimerization activity Source: MGI
small GTPase binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	229822, 21 interactors
CORUMⁱ	Q68FF6
IntActⁱ	Q68FF6, 31 interactors
Molecular INTeraction database More...MINTⁱ	Q68FF6
STRINGⁱ	10090.ENSMUSP00000037210

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q68FF6
SMRⁱ	Q68FF6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	1 – 124	Arf-GAPPROSITE-ProRule annotationAdd BLAST	124
Repeatⁱ	132 – 161	ANK 1Add BLAST	30
Repeatⁱ	166 – 195	ANK 2Add BLAST	30
Repeatⁱ	199 – 228	ANK 3Add BLAST	30

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	253 – 424	PTK2/FAK1-bindingBy similarityAdd BLAST	172
Regionⁱ	254 – 376	ARHGEF6-bindingBy similarityAdd BLAST	123
Regionⁱ	646 – 770	Interaction with PXN and TGFB1I1By similarityAdd BLAST	125

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	11 – 34	C4-typePROSITE-ProRule annotationAdd BLAST		24

Keywords - Domainⁱ

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG0818 Eukaryota ENOG410XR8U LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00930000150886
HOGENOMⁱ	HOG000232135
HOVERGENⁱ	HBG012506
InParanoidⁱ	Q68FF6
KEGG Orthology (KO) More...KOⁱ	K05737
OMAⁱ	QKCMSQS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G03VD
PhylomeDBⁱ	Q68FF6
TreeFamⁱ	TF317762

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00204 ANK, 1 hit
Gene3Dⁱ	1.25.40.20, 1 hit 3.30.40.160, 1 hit
InterProⁱ	View protein in InterPro IPR002110 Ankyrin_rpt IPR020683 Ankyrin_rpt-contain_dom IPR036770 Ankyrin_rpt-contain_sf IPR037278 ARFGAP/RecO IPR001164 ArfGAP_dom IPR038508 ArfGAP_dom_sf IPR032352 GIT1/2_CC IPR022018 GIT1_C IPR013724 GIT_SHD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12796 Ank_2, 1 hit PF01412 ArfGap, 1 hit PF12205 GIT1_C, 1 hit PF16559 GIT_CC, 1 hit PF08518 GIT_SHD, 2 hits
PRINTSⁱ	PR00405 REVINTRACTNG
SMARTⁱ	View protein in SMART SM00248 ANK, 3 hits SM00105 ArfGap, 1 hit SM00555 GIT, 2 hits
SUPFAMⁱ	SSF48403 SSF48403, 1 hit SSF57863 SSF57863, 1 hit
PROSITEⁱ	View protein in PROSITE PS50297 ANK_REP_REGION, 1 hit PS50088 ANK_REPEAT, 1 hit PS50115 ARFGAP, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q68FF6-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSRKGPRAEV CADCSAPDPG WASISRGVLV CDECCSVHRS LGRHISIVKH 
        60         70         80         90        100
LRHSAWPPTL LQMVHTLASN GANSIWEHSL LDPAQVQSGR RKANPQDKVH 
       110        120        130        140        150
PIKSEFIRAK YQMLAFVHKL PCRDDDGVTA KDLSKQLHSS VRTGNLETCL 
       160        170        180        190        200
RLLSLGAQAN FFHPEKGTTP LHVAAKAGQT LQAELLVVYG ADPGSPDVNG 
       210        220        230        240        250
RTPIDYARQA GHHELAERLV ECQYELTDRL AFYLCGRKPD HKNGHYIIPQ 
       260        270        280        290        300
MADRSRQKCM SQSLDLSELA KAAKKKLQAL SNRLFEELAM DVYDEVDRRE 
       310        320        330        340        350
NDAVWLATQN HSTLVTERSA VPFLPVNPEY SATRNQGRQK LARFNAREFA 
       360        370        380        390        400
TLIIDILSEA KRRQQGKSLS SPTDNLELSA RSQSELDDQH DYDSVASDED 
       410        420        430        440        450
TDQEPLPSAG ATRNNRARSM DSSDLSDGAV TLQEYLELKK ALATSEAKVQ 
       460        470        480        490        500
QLMKVNSSLS DELRRLQREI HKLQAENLQL RQPPGPVPPP SLPSERAEHT 
       510        520        530        540        550
LMGPGGSTHR RDRQAFSMYE PGSALKPFGG TPGDELATRL QPFHSTELED 
       560        570        580        590        600
DAIYSVHVPA GLYRIRKGVS ASSVPFTPSS PLLSCSQEGS RHASKLSRHG 
       610        620        630        640        650
SGADSDYENT QSGDPLLGLE GKRFLELSKE DELHPELESL DGDLDPGLPS 
       660        670        680        690        700
TEDVILKTEQ VTKNIQELLR AAQEFKHDSF VPCSEKIHLA VTEMASLFPK 
       710        720        730        740        750
RPALEPVRSS LRLLNASAYR LQSECRKTVP PEPGAPVDFQ LLTQQVIQCA 
       760        770
YDIAKAAKQL VTITTREKKQ

Length:770

Mass (Da):85,300

Last modified:October 11, 2004 - v1

Checksum:ⁱC516E7A49578D0B4

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	Q5F258	Q5F258_MOUSE		ARF GTPase-activating protein GIT1 ARF GTPase-activating protein GIT1	Git1	761	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL607072 Genomic DNA No translation available. BC079870 mRNA Translation: AAH79870.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS25080.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001004144.1, NM_001004144.1
UniGeneⁱ	Mm.290182

Genome annotation databases

Ensemblⁱ	ENSMUST00000037285; ENSMUSP00000037210; ENSMUSG00000011877
GeneIDⁱ	216963
KEGGⁱ	mmu:216963
UCSC genome browser More...UCSCⁱ	uc007kgy.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q68FF6	Uncharacterized protein		MOUSE		253	UniRef100_Q68FF6

Protein	Similar proteins		Species	Score	Length	Source
Q68FF6	ARF GTPase-activating protein GIT1	)	RAT		770	UniRef90_Q9Z272
ARF GTPase-activating protein GIT1		HUMAN		761
Uncharacterized protein		RHIBE		817
Uncharacterized protein		RHIRO		708
ARF GTPase-activating protein GIT1		CRIGR		809
+265

Protein	Similar proteins		Species	Score	Length	Source
Q68FF6	ARF GTPase-activating protein GIT1	)	RAT		770	UniRef50_Q9Z272
ARF GTPase-activating protein GIT1		HUMAN		761
Uncharacterized protein		RHIBE		817
Uncharacterized protein		RHIRO		708
ARF GTPase-activating protein GIT1		CRIGR		809
+268

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL607072 Genomic DNA No translation available. BC079870 mRNA Translation: AAH79870.1
CCDSⁱ	CCDS25080.1
RefSeqⁱ	NP_001004144.1, NM_001004144.1
UniGeneⁱ	Mm.290182

3D structure databases

ProteinModelPortalⁱ	Q68FF6
SMRⁱ	Q68FF6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	229822, 21 interactors
CORUMⁱ	Q68FF6
IntActⁱ	Q68FF6, 31 interactors
MINTⁱ	Q68FF6
STRINGⁱ	10090.ENSMUSP00000037210

PTM databases

iPTMnetⁱ	Q68FF6
PhosphoSitePlusⁱ	Q68FF6

Proteomic databases

EPDⁱ	Q68FF6
MaxQBⁱ	Q68FF6
PaxDbⁱ	Q68FF6
PeptideAtlasⁱ	Q68FF6
PRIDEⁱ	Q68FF6

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000037285; ENSMUSP00000037210; ENSMUSG00000011877
GeneIDⁱ	216963
KEGGⁱ	mmu:216963
UCSCⁱ	uc007kgy.1 mouse

Organism-specific databases

CTDⁱ	28964
MGIⁱ	MGI:1927140 Git1

Phylogenomic databases

eggNOGⁱ	KOG0818 Eukaryota ENOG410XR8U LUCA
GeneTreeⁱ	ENSGT00930000150886
HOGENOMⁱ	HOG000232135
HOVERGENⁱ	HBG012506
InParanoidⁱ	Q68FF6
KOⁱ	K05737
OMAⁱ	QKCMSQS
OrthoDBⁱ	EOG091G03VD
PhylomeDBⁱ	Q68FF6
TreeFamⁱ	TF317762

Enzyme and pathway databases

Reactomeⁱ	R-MMU-3928664 Ephrin signaling

Reactomeⁱ

R-MMU-3928664 Ephrin signaling

Miscellaneous databases

ChiTaRSⁱ	Git1 mouse
Protein Ontology More...PROⁱ	PR:Q68FF6
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000011877 Expressed in 271 organ(s), highest expression level in cardiac ventricle
CleanExⁱ	MM_GIT1
ExpressionAtlasⁱ	Q68FF6 baseline and differential
Genevisibleⁱ	Q68FF6 MM

Family and domain databases

CDDⁱ	cd00204 ANK, 1 hit
Gene3Dⁱ	1.25.40.20, 1 hit 3.30.40.160, 1 hit
InterProⁱ	View protein in InterPro IPR002110 Ankyrin_rpt IPR020683 Ankyrin_rpt-contain_dom IPR036770 Ankyrin_rpt-contain_sf IPR037278 ARFGAP/RecO IPR001164 ArfGAP_dom IPR038508 ArfGAP_dom_sf IPR032352 GIT1/2_CC IPR022018 GIT1_C IPR013724 GIT_SHD
Pfamⁱ	View protein in Pfam PF12796 Ank_2, 1 hit PF01412 ArfGap, 1 hit PF12205 GIT1_C, 1 hit PF16559 GIT_CC, 1 hit PF08518 GIT_SHD, 2 hits
PRINTSⁱ	PR00405 REVINTRACTNG
SMARTⁱ	View protein in SMART SM00248 ANK, 3 hits SM00105 ArfGap, 1 hit SM00555 GIT, 2 hits
SUPFAMⁱ	SSF48403 SSF48403, 1 hit SSF57863 SSF57863, 1 hit
PROSITEⁱ	View protein in PROSITE PS50297 ANK_REP_REGION, 1 hit PS50088 ANK_REPEAT, 1 hit PS50115 ARFGAP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GIT1_MOUSE
Accessionⁱ	Q68FF6Primary (citable) accession number: Q68FF6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
	Last sequence update:	October 11, 2004
	Last modified:	November 7, 2018
	This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q68FF6 (GIT1_MOUSE)

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ARF GTPase-activating protein GIT1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Endosome

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ