UniProtKB - Q68EF4 (GRM4_MOUSE)
Protein
Metabotropic glutamate receptor 4
Gene
Grm4
Organism
Mus musculus (Mouse)
Status
Functioni
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 159 | GlutamateBy similarity | 1 | |
Binding sitei | 230 | GlutamateBy similarity | 1 | |
Binding sitei | 312 | GlutamateBy similarity | 1 | |
Binding sitei | 405 | GlutamateBy similarity | 1 |
GO - Molecular functioni
- calcium-dependent protein binding Source: MGI
- calmodulin binding Source: MGI
- glutamate receptor activity Source: UniProtKB
- G protein-coupled receptor activity Source: UniProtKB
- group III metabotropic glutamate receptor activity Source: GO_Central
GO - Biological processi
- activation of MAPK activity Source: MGI
- adenylate cyclase-inhibiting G protein-coupled glutamate receptor signaling pathway Source: UniProtKB
- chemical synaptic transmission Source: MGI
- G protein-coupled glutamate receptor signaling pathway Source: GO_Central
- learning Source: MGI
- regulation of synaptic transmission, glutamatergic Source: GO_Central
- regulation of synaptic vesicle exocytosis Source: SynGO
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events R-MMU-420499, Class C/3 (Metabotropic glutamate/pheromone receptors) |
Names & Taxonomyi
Protein namesi | Recommended name: Metabotropic glutamate receptor 4Short name: mGluR4 |
Gene namesi | Name:Grm4 Synonyms:Gprc1d, Mglur4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351341, Grm4 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: MGI
- integral component of presynaptic active zone membrane Source: SynGO
- presynaptic active zone membrane Source: MGI
Other locations
- cytoplasmic vesicle Source: MGI
- dendritic shaft Source: MGI
- glutamatergic synapse Source: SynGO
- neuron projection Source: BHF-UCL
- parallel fiber to Purkinje cell synapse Source: SynGO
- terminal bouton Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 33 – 586 | ExtracellularSequence analysisAdd BLAST | 554 | |
Transmembranei | 587 – 607 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 608 – 624 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 625 – 645 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 646 – 653 | ExtracellularSequence analysis | 8 | |
Transmembranei | 654 – 671 | Helical; Name=3Sequence analysisAdd BLAST | 18 | |
Topological domaini | 672 – 699 | CytoplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 700 – 720 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 721 – 751 | ExtracellularSequence analysisAdd BLAST | 31 | |
Transmembranei | 752 – 772 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 773 – 786 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 787 – 807 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 808 – 826 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 827 – 847 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 848 – 912 | CytoplasmicSequence analysisAdd BLAST | 65 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 32 | Sequence analysisAdd BLAST | 32 | |
ChainiPRO_0000306852 | 33 – 912 | Metabotropic glutamate receptor 4Add BLAST | 880 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 67 ↔ 109 | By similarity | ||
Glycosylationi | 98 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 249 ↔ 538 | By similarity | ||
Glycosylationi | 301 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 372 ↔ 388 | By similarity | ||
Disulfide bondi | 428 ↔ 435 | By similarity | ||
Disulfide bondi | 520 ↔ 539 | By similarity | ||
Disulfide bondi | 524 ↔ 542 | By similarity | ||
Disulfide bondi | 545 ↔ 557 | By similarity | ||
Disulfide bondi | 560 ↔ 573 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q68EF4 |
PeptideAtlasi | Q68EF4 |
PRIDEi | Q68EF4 |
PTM databases
GlyConnecti | 2509, 2 N-Linked glycans (1 site) |
GlyGeni | Q68EF4, 3 sites |
iPTMneti | Q68EF4 |
PhosphoSitePlusi | Q68EF4 |
Interactioni
Subunit structurei
Interacts with PICK1.
By similarityGO - Molecular functioni
- calcium-dependent protein binding Source: MGI
- calmodulin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 234582, 4 interactors |
DIPi | DIP-49002N |
IntActi | Q68EF4, 2 interactors |
STRINGi | 10090.ENSMUSP00000112578 |
Chemistry databases
BindingDBi | Q68EF4 |
Miscellaneous databases
RNActi | Q68EF4, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 180 – 182 | Glutamate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the G-protein coupled receptor 3 family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1056, Eukaryota |
InParanoidi | Q68EF4 |
PhylomeDBi | Q68EF4 |
Family and domain databases
Gene3Di | 2.10.50.30, 1 hit |
InterProi | View protein in InterPro IPR001828, ANF_lig-bd_rcpt IPR000337, GPCR_3 IPR011500, GPCR_3_9-Cys_dom IPR038550, GPCR_3_9-Cys_sf IPR017978, GPCR_3_C IPR017979, GPCR_3_CS IPR000162, GPCR_3_mtglu_rcpt IPR001786, GPCR_3_mtglu_rcpt_4 IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF00003, 7tm_3, 1 hit PF01094, ANF_receptor, 1 hit PF07562, NCD3G, 1 hit |
PRINTSi | PR00248, GPCRMGR PR01054, MTABOTROPC4R PR00593, MTABOTROPICR |
SUPFAMi | SSF53822, SSF53822, 1 hit |
PROSITEi | View protein in PROSITE PS00979, G_PROTEIN_RECEP_F3_1, 1 hit PS00980, G_PROTEIN_RECEP_F3_2, 1 hit PS00981, G_PROTEIN_RECEP_F3_3, 1 hit PS50259, G_PROTEIN_RECEP_F3_4, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q68EF4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGKGGWAWW WARLPLCLLL SLYGSWVPSS LGKPKGHPHM NSIRIDGDIT
60 70 80 90 100
LGGLFPVHGR GSEGKACGEL KKEKGIHRLE AMLFALDRIN NDPDLLPNIT
110 120 130 140 150
LGARILDTCS RDTHALEQSL TFVQALIEKD GTEVRCGSGG PPIITKPERV
160 170 180 190 200
VGVIGASGSS VSIMVANILR LFKIPQISYA STAPDLSDNS RYDFFSRVVP
210 220 230 240 250
SDTYQAQAMV DIVRALKWNY VSTLASEGSY GESGVEAFIQ KSRENGGVCI
260 270 280 290 300
AQSVKIPREP KTGEFDKIIK RLLETSNARA IIIFANEDDI RRVLEAARRA
310 320 330 340 350
NQTGHFFWMG SDSWGSKSAP VLRLEEVAEG AVTILPKRTS VRGFDRYFSS
360 370 380 390 400
RTLDNNRRNI WFAEFWEDNF HCKLSRHALK KGSHIKKCTN RERIGQDSAY
410 420 430 440 450
EQEGKVQFVI DAVYAMGHAL HAMHRDLCPG RVGLCPRMDP VDGTQLLKYI
460 470 480 490 500
RNVNFSGIAG NPVTFNENGD APGRYDIYQY QRRNGSAEYK VIGSWTDHLH
510 520 530 540 550
LRIERMQWPG SGQQLPRSIC SLPCQPGERK KTVKGMACCW HCEPCTGYQY
560 570 580 590 600
QVDRYTCKTC PYDMRPTENR TSCQPIPIVK LEWDSPWAVL PLFLAVVGIA
610 620 630 640 650
ATLFVVVTFV RYNDTPIVKA SGRELSYVLL AGIFLCYATT FLMIAEPDLG
660 670 680 690 700
TCSLRRIFLG LGMSISYAAL LTKTNRIYRI FEQGKRSVSA PRFISPASQL
710 720 730 740 750
AITFVLISLQ LLCICVWFVV DPSHSVVDFQ DQRTLDPRFA RGVLKCDISD
760 770 780 790 800
LSLICLLGYS MLLMVTCTVY AIKTRGVPET FNEAKPIGFT MYTTCIVWLA
810 820 830 840 850
FIPIFFGTSQ SADKLYIQTT TLTVSVSLSA SVSLGMLYMP KVYIILFHPE
860 870 880 890 900
QNVPKRKRSL KAVVTAATMS NKFTQKGNFR PNGEAKSELC ENLEAPALAT
910
KQTYVTYTNH AI
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3XA00 | G3XA00_MOUSE | Metabotropic glutamate receptor 4 | Grm4 | 912 | Annotation score: | ||
A0A140T8R6 | A0A140T8R6_MOUSE | Metabotropic glutamate receptor 4 | Grm4 | 832 | Annotation score: | ||
A0A338P7K6 | A0A338P7K6_MOUSE | Metabotropic glutamate receptor 4 | Grm4 | 865 | Annotation score: | ||
A0A338P783 | A0A338P783_MOUSE | Metabotropic glutamate receptor 4 | Grm4 | 657 | Annotation score: | ||
A0A338P6S6 | A0A338P6S6_MOUSE | Metabotropic glutamate receptor 4 | Grm4 | 181 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 713 | C → G in AAH80284 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_028517 | 815 – 832 | LYIQT…LSASV → VTSEALPVEFSPPLLAHN in isoform 2. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_028518 | 833 – 912 | Missing in isoform 2. 1 PublicationAdd BLAST | 80 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC072635 mRNA No translation available. BC080284 mRNA Translation: AAH80284.1 AY255558 mRNA Translation: AAO85070.1 |
CCDSi | CCDS28563.1 [Q68EF4-2] CCDS70775.1 [Q68EF4-1] |
RefSeqi | NP_001013403.1, NM_001013385.2 |
Genome annotation databases
GeneIDi | 268934 |
KEGGi | mmu:268934 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC072635 mRNA No translation available. BC080284 mRNA Translation: AAH80284.1 AY255558 mRNA Translation: AAO85070.1 |
CCDSi | CCDS28563.1 [Q68EF4-2] CCDS70775.1 [Q68EF4-1] |
RefSeqi | NP_001013403.1, NM_001013385.2 |
3D structure databases
SMRi | Q68EF4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 234582, 4 interactors |
DIPi | DIP-49002N |
IntActi | Q68EF4, 2 interactors |
STRINGi | 10090.ENSMUSP00000112578 |
Chemistry databases
BindingDBi | Q68EF4 |
ChEMBLi | CHEMBL4105794 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyConnecti | 2509, 2 N-Linked glycans (1 site) |
GlyGeni | Q68EF4, 3 sites |
iPTMneti | Q68EF4 |
PhosphoSitePlusi | Q68EF4 |
Proteomic databases
PaxDbi | Q68EF4 |
PeptideAtlasi | Q68EF4 |
PRIDEi | Q68EF4 |
Genome annotation databases
GeneIDi | 268934 |
KEGGi | mmu:268934 |
Organism-specific databases
CTDi | 2914 |
MGIi | MGI:1351341, Grm4 |
Phylogenomic databases
eggNOGi | KOG1056, Eukaryota |
InParanoidi | Q68EF4 |
PhylomeDBi | Q68EF4 |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events R-MMU-420499, Class C/3 (Metabotropic glutamate/pheromone receptors) |
Miscellaneous databases
BioGRID-ORCSi | 268934, 1 hit in 17 CRISPR screens |
ChiTaRSi | Grm4, mouse |
PROi | PR:Q68EF4 |
RNActi | Q68EF4, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 2.10.50.30, 1 hit |
InterProi | View protein in InterPro IPR001828, ANF_lig-bd_rcpt IPR000337, GPCR_3 IPR011500, GPCR_3_9-Cys_dom IPR038550, GPCR_3_9-Cys_sf IPR017978, GPCR_3_C IPR017979, GPCR_3_CS IPR000162, GPCR_3_mtglu_rcpt IPR001786, GPCR_3_mtglu_rcpt_4 IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF00003, 7tm_3, 1 hit PF01094, ANF_receptor, 1 hit PF07562, NCD3G, 1 hit |
PRINTSi | PR00248, GPCRMGR PR01054, MTABOTROPC4R PR00593, MTABOTROPICR |
SUPFAMi | SSF53822, SSF53822, 1 hit |
PROSITEi | View protein in PROSITE PS00979, G_PROTEIN_RECEP_F3_1, 1 hit PS00980, G_PROTEIN_RECEP_F3_2, 1 hit PS00981, G_PROTEIN_RECEP_F3_3, 1 hit PS50259, G_PROTEIN_RECEP_F3_4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GRM4_MOUSE | |
Accessioni | Q68EF4Primary (citable) accession number: Q68EF4 Secondary accession number(s): Q80UC2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | October 2, 2007 | |
Last modified: | December 2, 2020 | |
This is version 115 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries