UniProtKB - Q68DD2 (PA24F_HUMAN)
Cytosolic phospholipase A2 zeta
PLA2G4F
Functioni
Has calcium-dependent phospholipase and lysophospholipase activities with a potential role in membrane lipid remodeling and biosynthesis of lipid mediators (PubMed:29158256).
Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) (PubMed:29158256).
Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis (PubMed:29158256).
In myocardial mitochondria, plays a major role in arachidonate release that is metabolically channeled to the formation of cardioprotective eicosanoids, epoxyeicosatrienoates (EETs) (PubMed:29158256).
1 PublicationCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+1 PublicationEC:3.1.1.41 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H+ + hexadecanoateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-2-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 2-O-hexadecyl-sn-glycero-3-phosphocholine + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-O-hexadecyl-sn-glycero-3-phosphocholine + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H+ + hexadecanoate + sn-glycerol 3-phosphocholineBy similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 60 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 60 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 66 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 116 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 116 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 118 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 118 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 123 | Calcium 2PROSITE-ProRule annotation | 1 | |
Active sitei | 395 | NucleophileBy similarity | 1 | |
Active sitei | 680 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- calcium-dependent phospholipase A2 activity Source: GO_Central
- calcium-dependent phospholipid binding Source: GO_Central
- calcium ion binding Source: GO_Central
- lysophospholipase activity Source: Ensembl
- phospholipase A1 activity Source: Reactome
- phospholipase A2 activity Source: GO_Central
GO - Biological processi
- arachidonic acid secretion Source: Ensembl
- cellular response to antibiotic Source: Ensembl
- cellular response to organic cyclic compound Source: Ensembl
- glycerophospholipid catabolic process Source: GO_Central
- phosphatidylglycerol acyl-chain remodeling Source: Reactome
- prostaglandin biosynthetic process Source: Ensembl
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid degradation, Lipid metabolism, Phospholipid degradation, Phospholipid metabolism |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q68DD2 |
Reactomei | R-HSA-1482788, Acyl chain remodelling of PC R-HSA-1482801, Acyl chain remodelling of PS R-HSA-1482839, Acyl chain remodelling of PE R-HSA-1482922, Acyl chain remodelling of PI R-HSA-1482925, Acyl chain remodelling of PG R-HSA-1483115, Hydrolysis of LPC |
SignaLinki | Q68DD2 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic phospholipase A2 zeta (EC:3.1.1.41 Publication)Short name: cPLA2-zeta Alternative name(s): Phospholipase A2 group IVF |
Gene namesi | Name:PLA2G4F |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:27396, PLA2G4F |
neXtProti | NX_Q68DD2 |
VEuPathDBi | HostDB:ENSG00000168907 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol By similarity
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein Curated
Mitochondrion
- Mitochondrion 1 Publication
Cytosol
- cytosol Source: GO_Central
Mitochondrion
- mitochondrion Source: UniProtKB-SubCell
Plasma Membrane
- ruffle membrane Source: GO_Central
Other locations
- vesicle Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, MitochondrionPathology & Biotechi
Organism-specific databases
DisGeNETi | 255189 |
OpenTargetsi | ENSG00000168907 |
PharmGKBi | PA142671170 |
Miscellaneous databases
Pharosi | Q68DD2, Tdark |
Genetic variation databases
BioMutai | PLA2G4F |
DMDMi | 317373489 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000247027 | 1 – 849 | Cytosolic phospholipase A2 zetaAdd BLAST | 849 |
Proteomic databases
jPOSTi | Q68DD2 |
MassIVEi | Q68DD2 |
MaxQBi | Q68DD2 |
PaxDbi | Q68DD2 |
PeptideAtlasi | Q68DD2 |
PRIDEi | Q68DD2 |
ProteomicsDBi | 66075 [Q68DD2-1] 66076 [Q68DD2-2] 66077 [Q68DD2-3] |
PTM databases
iPTMneti | Q68DD2 |
PhosphoSitePlusi | Q68DD2 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000168907, Expressed in zone of skin and 122 other tissues |
ExpressionAtlasi | Q68DD2, baseline and differential |
Genevisiblei | Q68DD2, HS |
Organism-specific databases
HPAi | ENSG00000168907, Tissue enriched (skin) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 129083, 9 interactors |
IntActi | Q68DD2, 10 interactors |
STRINGi | 9606.ENSP00000380442 |
Miscellaneous databases
RNActi | Q68DD2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 145 | C2PROSITE-ProRule annotationAdd BLAST | 119 | |
Domaini | 306 – 849 | PLA2cPROSITE-ProRule annotationAdd BLAST | 544 |
Domaini
Phylogenomic databases
eggNOGi | KOG1028, Eukaryota KOG1325, Eukaryota |
GeneTreei | ENSGT01030000234606 |
HOGENOMi | CLU_011663_0_0_1 |
InParanoidi | Q68DD2 |
OMAi | WPEPRIC |
PhylomeDBi | Q68DD2 |
TreeFami | TF325228 |
Family and domain databases
CDDi | cd04036, C2_cPLA2, 1 hit |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR041847, C2_cPLA2 IPR000008, C2_dom IPR035892, C2_domain_sf IPR040723, cPLA2_C2 IPR002642, LysoPLipase_cat_dom |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF18695, cPLA2_C2, 1 hit PF01735, PLA2_B, 1 hit |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00022, PLAc, 1 hit |
SUPFAMi | SSF49562, SSF49562, 1 hit SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS51210, PLA2C, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLWALWPRWL ADKMLPLLGA VLLQKREKRG PLWRHWRRET YPYYDLQVKV
60 70 80 90 100
LRATNIRGTD LLSKADCYVQ LWLPTASPSP AQTRIVANCS DPEWNETFHY
110 120 130 140 150
QIHGAVKNVL ELTLYDKDIL GSDQLSLLLF DLRSLKCGQP HKHTFPLNHQ
160 170 180 190 200
DSQELQVEFV LEKSQVPASE VITNGVLVAH PCLRIQGTLR GDGTAPREEY
210 220 230 240 250
GSRQLQLAVP GAYEKPQLLP LQPPTEPGLP PTFTFHVNPV LSSRLHVELM
260 270 280 290 300
ELLAAVQSGP SAELEAQTSK LGEGGILLSS LPLGQEEQCS VALGEGQEVA
310 320 330 340 350
LSMKVEMSSG DLDLRLGFDL SDGEQEFLDR RKQVVSKALQ QVLGLSEALD
360 370 380 390 400
SGQVPVVAVL GSGGGTRAMS SLYGSLAGLQ ELGLLDTVTY LSGVSGSTWC
410 420 430 440 450
ISTLYRDPAW SQVALQGPIE RAQVHVCSSK MGALSTERLQ YYTQELGVRE
460 470 480 490 500
RSGHSVSLID LWGLLVEYLL YQEENPAKLS DQQEAVRQGQ NPYPIYTSVN
510 520 530 540 550
VRTNLSGEDF AEWCEFTPYE VGFPKYGAYV PTELFGSELF MGRLLQLQPE
560 570 580 590 600
PRICYLQGMW GSAFATSLDE IFLKTAGSGL SFLEWYRGSV NITDDCQKPQ
610 620 630 640 650
LHNPSRLRTR LLTPQGPFSQ AVLDIFTSRF TSAQSFNFTR GLCLHKDYVA
660 670 680 690 700
GREFVAWKDT HPDAFPNQLT PMRDCLYLVD GGFAINSPFP LALLPQRAVD
710 720 730 740 750
LILSFDYSLE APFEVLKMTE KYCLDRGIPF PSIEVGPEDM EEARECYLFA
760 770 780 790 800
KAEDPRSPIV LHFPLVNRTF RTHLAPGVER QTAEEKAFGD FVINRPDTPY
810 820 830 840
GMMNFTYEPQ DFYRLVALSR YNVLNNVETL KCALQLALDR HQARERAGA
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BMD0 | H3BMD0_HUMAN | Cytosolic phospholipase A2 zeta | PLA2G4F | 296 | Annotation score: | ||
H3BSG9 | H3BSG9_HUMAN | Cytosolic phospholipase A2 zeta | PLA2G4F | 119 | Annotation score: | ||
H7BXJ8 | H7BXJ8_HUMAN | Cytosolic phospholipase A2 zeta | PLA2G4F | 60 | Annotation score: | ||
H3BPX7 | H3BPX7_HUMAN | Cytosolic phospholipase A2 zeta | PLA2G4F | 70 | Annotation score: | ||
H3BNW4 | H3BNW4_HUMAN | Cytosolic phospholipase A2 zeta | PLA2G4F | 86 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 203 | R → G in BAD18801 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 262 | A → T in BAD18801 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 363 | G → E in CAH18288 (PubMed:17974005).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_053553 | 30 | G → V. Corresponds to variant dbSNP:rs636604Ensembl. | 1 | |
Natural variantiVAR_027054 | 740 | M → V1 PublicationCorresponds to variant dbSNP:rs1356410Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019887 | 352 | G → GQV in isoform 3. 1 Publication | 1 | |
Alternative sequenceiVSP_019888 | 433 – 454 | ALSTE…ERSGH → DVRVSPCQLPRLHSSNLDHS LW in isoform 2. 1 PublicationAdd BLAST | 22 | |
Alternative sequenceiVSP_019889 | 455 – 849 | Missing in isoform 2. 1 PublicationAdd BLAST | 395 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK172836 mRNA Translation: BAD18801.1 AC036103 Genomic DNA No translation available. CR749451 mRNA Translation: CAH18288.1 |
CCDSi | CCDS32204.1 [Q68DD2-1] |
RefSeqi | NP_998765.3, NM_213600.3 [Q68DD2-1] |
Genome annotation databases
Ensembli | ENST00000397272.7; ENSP00000380442.4; ENSG00000168907.13 |
GeneIDi | 255189 |
KEGGi | hsa:255189 |
MANE-Selecti | ENST00000397272.7; ENSP00000380442.4; NM_213600.4; NP_998765.3 |
UCSCi | uc001zoz.4, human [Q68DD2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK172836 mRNA Translation: BAD18801.1 AC036103 Genomic DNA No translation available. CR749451 mRNA Translation: CAH18288.1 |
CCDSi | CCDS32204.1 [Q68DD2-1] |
RefSeqi | NP_998765.3, NM_213600.3 [Q68DD2-1] |
3D structure databases
AlphaFoldDBi | Q68DD2 |
SMRi | Q68DD2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 129083, 9 interactors |
IntActi | Q68DD2, 10 interactors |
STRINGi | 9606.ENSP00000380442 |
PTM databases
iPTMneti | Q68DD2 |
PhosphoSitePlusi | Q68DD2 |
Genetic variation databases
BioMutai | PLA2G4F |
DMDMi | 317373489 |
Proteomic databases
jPOSTi | Q68DD2 |
MassIVEi | Q68DD2 |
MaxQBi | Q68DD2 |
PaxDbi | Q68DD2 |
PeptideAtlasi | Q68DD2 |
PRIDEi | Q68DD2 |
ProteomicsDBi | 66075 [Q68DD2-1] 66076 [Q68DD2-2] 66077 [Q68DD2-3] |
Protocols and materials databases
Antibodypediai | 42117, 68 antibodies from 25 providers |
DNASUi | 255189 |
Genome annotation databases
Ensembli | ENST00000397272.7; ENSP00000380442.4; ENSG00000168907.13 |
GeneIDi | 255189 |
KEGGi | hsa:255189 |
MANE-Selecti | ENST00000397272.7; ENSP00000380442.4; NM_213600.4; NP_998765.3 |
UCSCi | uc001zoz.4, human [Q68DD2-1] |
Organism-specific databases
CTDi | 255189 |
DisGeNETi | 255189 |
GeneCardsi | PLA2G4F |
HGNCi | HGNC:27396, PLA2G4F |
HPAi | ENSG00000168907, Tissue enriched (skin) |
neXtProti | NX_Q68DD2 |
OpenTargetsi | ENSG00000168907 |
PharmGKBi | PA142671170 |
VEuPathDBi | HostDB:ENSG00000168907 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1028, Eukaryota KOG1325, Eukaryota |
GeneTreei | ENSGT01030000234606 |
HOGENOMi | CLU_011663_0_0_1 |
InParanoidi | Q68DD2 |
OMAi | WPEPRIC |
PhylomeDBi | Q68DD2 |
TreeFami | TF325228 |
Enzyme and pathway databases
PathwayCommonsi | Q68DD2 |
Reactomei | R-HSA-1482788, Acyl chain remodelling of PC R-HSA-1482801, Acyl chain remodelling of PS R-HSA-1482839, Acyl chain remodelling of PE R-HSA-1482922, Acyl chain remodelling of PI R-HSA-1482925, Acyl chain remodelling of PG R-HSA-1483115, Hydrolysis of LPC |
SignaLinki | Q68DD2 |
Miscellaneous databases
BioGRID-ORCSi | 255189, 6 hits in 1066 CRISPR screens |
GenomeRNAii | 255189 |
Pharosi | Q68DD2, Tdark |
PROi | PR:Q68DD2 |
RNActi | Q68DD2, protein |
Gene expression databases
Bgeei | ENSG00000168907, Expressed in zone of skin and 122 other tissues |
ExpressionAtlasi | Q68DD2, baseline and differential |
Genevisiblei | Q68DD2, HS |
Family and domain databases
CDDi | cd04036, C2_cPLA2, 1 hit |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR016035, Acyl_Trfase/lysoPLipase IPR041847, C2_cPLA2 IPR000008, C2_dom IPR035892, C2_domain_sf IPR040723, cPLA2_C2 IPR002642, LysoPLipase_cat_dom |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF18695, cPLA2_C2, 1 hit PF01735, PLA2_B, 1 hit |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00022, PLAc, 1 hit |
SUPFAMi | SSF49562, SSF49562, 1 hit SSF52151, SSF52151, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS51210, PLA2C, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PA24F_HUMAN | |
Accessioni | Q68DD2Primary (citable) accession number: Q68DD2 Secondary accession number(s): Q6ZMC8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | January 11, 2011 | |
Last modified: | May 25, 2022 | |
This is version 132 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports