SIZ1

Functionⁱ

E3 SUMO protein ligase involved in regulation processes. Mediates SUMO/ attachment to PHR1, a MYB transcriptional activator controlling the phosphate deficiency responses (PubMed:15894620). Functions as an upstream negative regulator of salicylic acid (SA) accumulation and subsequent SA-mediated systemic acquired resistance (SAR) signaling. Probably not involved in jasmonic acid (JA)-mediated defense response. Participates in abiotic stress-induced sumoylation. Controls heat shock-induced SUMO1 and SUMO2 conjugation and facilitates basal thermotolerance. Involved in freezing tolerance by mediating sumoylation of ICE1, a transcription activator of the cold signaling regulator CBF3/DREB1A. Acts as positive regulator of drought stress tolerance. Acts as floral repressor that promotes FLC expression by repressing FLD activity through sumoylation. Acts as negative regulator of abscisic acid (ABA) signaling through ABI5 sumoylation. Mediates sumoylation of SCE1, GTE3 and GTE5. Functions as negative regulator of SnRK1 signaling through sumoylation of several components of the SnRK1 complex (PubMed:26662259).10 Publications

Pathwayⁱ: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	112 – 168	PHD-typeAdd BLAST		57
Zinc fingerⁱ	348 – 425	SP-RING-typePROSITE-ProRule annotationAdd BLAST		78

GO - Molecular functionⁱ

SUMO transferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 18069938
- 18502747
zinc ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell division
Source: TAIR
Inferred from mutant phenotypeⁱ
- 20007967
cellular response to phosphate starvation
Source: TAIR
Inferred from mutant phenotypeⁱ
- 21156857
defense response Source: UniProtKB-KW
detection of phosphate ion
Source: TAIR
Inferred from direct assayⁱ
- 21156857
developmental growth
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17905899
embryo sac development
Source: TAIR
Inferred from mutant phenotypeⁱ
- 22253727
heat acclimation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17041025
negative regulation of flower development
Source: TAIR
Inferred from mutant phenotypeⁱ
- 18069938
negative regulation of systemic acquired resistance
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17163880
plant ovule development
Source: TAIR
Inferred from mutant phenotypeⁱ
- 22253727
pollen tube guidance
Source: TAIR
Inferred from mutant phenotypeⁱ
- 22253727
protein sumoylation
Source: UniProtKB
Inferred from direct assayⁱ
- 18069938
- 18502747
regulation of abscisic acid-activated signaling pathway
Source: TAIR
Inferred from mutant phenotypeⁱ
- 19276109
regulation of growth
Source: TAIR
Inferred from mutant phenotypeⁱ
- 17905899
regulation of nitrate assimilation
Source: TAIR
Inferred from mutant phenotypeⁱ
- 21772271
regulation of response to water deprivation
Source: TAIR
Inferred from mutant phenotypeⁱ
- 17905899
regulation of salicylic acid metabolic process
Source: TAIR
Inferred from mutant phenotypeⁱ
- 20007967
response to freezing
Source: TAIR
Inferred from mutant phenotypeⁱ
- 17416732
response to water deprivation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17905899
unidimensional cell growth
Source: TAIR
Inferred from mutant phenotypeⁱ
- 20007967

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Flowering, Plant defense, Stress response, Ubl conjugation pathway
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-ATH-3108214 SUMOylation of DNA damage response and repair proteins R-ATH-3899300 SUMOylation of transcription cofactors R-ATH-4085377 SUMOylation of SUMOylation proteins R-ATH-4615885 SUMOylation of DNA replication proteins R-ATH-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-ATH-5696395 Formation of Incision Complex in GG-NER
UniPathwayⁱ	UPA00886

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: E3 SUMO-protein ligase SIZ11 Publication (EC:2.3.2.-Curated) Alternative name(s): E3 SUMO-protein transferase SIZ1Curated
Gene namesⁱ	Name:SIZ11 Publication Ordered Locus Names:At5g60410Imported ORF Names:MUF9.5Imported, MUF9.70
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 5

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT5G60410
TAIRⁱ	locus:2175148 AT5G60410

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Dwarf phenotype. Heat-sensitive phenotype. Early flowering under short day. Elevated level of salicylic acid (SA), increased expression of pathogenesis-related (PR) genes and increased resistance to the bacterial pathogen P.syringae. ABA hypersensitivity during seed germination primary root growth. Exaggerated prototypical Pi-starvation responses, including increased root-shoot mass ratio and greater anthocyanin accumulation (PubMed:15894620).7 Publications

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	100	K → R: Strongly reduces autosumoylation; when associated with R-488. 1 Publication	1
Mutagenesisⁱ	379	C → A: Abolishes activity. 1 Publication	1
Mutagenesisⁱ	488	K → R: Strongly reduces autosumoylation; when associated with R-100. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000218984	1 – 884	E3 SUMO-protein ligase SIZ1Add BLAST		884

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Cross-linkⁱ	100	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linkⁱ	488	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modificationⁱ

Autosumoylated at Lys-100 and Lys-488.

Keywords - PTMⁱ

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q680Q4
PRIDEⁱ	Q680Q4

PTM databases

iPTMnetⁱ	Q680Q4

iPTMnetⁱ

Q680Q4

Expressionⁱ

Tissue specificityⁱ

Ubiquitous.1 Publication

Gene expression databases

ExpressionAtlasⁱ	Q680Q4 baseline and differential
Genevisibleⁱ	Q680Q4 AT

Interactionⁱ

Subunit structureⁱ

Interacts (via PHD domain) with SCE1, GTE3 and GTE5.1 Publication

Protein-protein interaction databases

BioGridⁱ	21407, 17 interactors
STRINGⁱ	3702.AT5G60410.2

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	106 – 108	Combined sources	3
Beta strandⁱ	126 – 128	Combined sources	3
Turnⁱ	132 – 134	Combined sources	3
Beta strandⁱ	137 – 139	Combined sources	3
Helixⁱ	140 – 143	Combined sources	4
Turnⁱ	148 – 150	Combined sources	3
Helixⁱ	163 – 167	Combined sources	5

Show more details

3D structure databases

ProteinModelPortalⁱ	Q680Q4
SMRⁱ	Q680Q4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

Q680Q4

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	11 – 45	SAPPROSITE-ProRule annotationAdd BLAST		35

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	336 – 340	Poly-Gly		5

Domainⁱ

The PHD-type zinc finger mediates interaction with SCE1, GTE3 and GTE5 and is required for E3 activity.1 Publication

Sequence similaritiesⁱ

Belongs to the PIAS family.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	112 – 168	PHD-typeAdd BLAST		57
Zinc fingerⁱ	348 – 425	SP-RING-typePROSITE-ProRule annotationAdd BLAST		78

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG2169 Eukaryota ENOG410XQ2E LUCA
HOGENOMⁱ	HOG000029457
InParanoidⁱ	Q680Q4
OMAⁱ	EHDSISM
PhylomeDBⁱ	Q680Q4

Family and domain databases

Gene3Dⁱ	1.10.720.30, 1 hit 3.30.40.10, 2 hits
InterProⁱ	View protein in InterPro IPR003034 SAP_dom IPR036361 SAP_dom_sf IPR031141 SIZ1_plant IPR019786 Zinc_finger_PHD-type_CS IPR011011 Znf_FYVE_PHD IPR004181 Znf_MIZ IPR001965 Znf_PHD IPR013083 Znf_RING/FYVE/PHD
PANTHERⁱ	PTHR10782:SF34 PTHR10782:SF34, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02037 SAP, 1 hit PF02891 zf-MIZ, 1 hit
SMARTⁱ	View protein in SMART SM00249 PHD, 1 hit SM00513 SAP, 1 hit
SUPFAMⁱ	SSF57903 SSF57903, 1 hit SSF68906 SSF68906, 1 hit
PROSITEⁱ	View protein in PROSITE PS50800 SAP, 1 hit PS01359 ZF_PHD_1, 1 hit PS51044 ZF_SP_RING, 1 hit

Sequences (4)ⁱ

Sequence statusⁱ: Complete.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket

Isoform 1 (identifier: Q680Q4-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLEANCKEK LSYFRIKELK DVLTQLGLSK QGKKQELVDR ILTLLSDEQA 
        60         70         80         90        100
ARLLSKKNTV AKEAVAKLVD DTYRKMQVSG ASDLASKGQV SSDTSNLKVK 
       110        120        130        140        150
GEPEDPFQPE IKVRCVCGNS LETDSMIQCE DPRCHVWQHV GCVILPDKPM 
       160        170        180        190        200
DGNPPLPESF YCEICRLTRA DPFWVTVAHP LSPVRLTATT IPNDGASTMQ 
       210        220        230        240        250
SVERTFQITR ADKDLLAKPE YDVQAWCMLL NDKVLFRMQW PQYADLQVNG 
       260        270        280        290        300
VPVRAINRPG GQLLGVNGRD DGPIITSCIR DGVNRISLSG GDVRIFCFGV 
       310        320        330        340        350
RLVKRRTLQQ VLNLIPEEGK GETFEDALAR VRRCIGGGGG DDNADSDSDI 
       360        370        380        390        400
EVVADFFGVN LRCPMSGSRI KVAGRFLPCV HMGCFDLDVF VELNQRSRKW 
       410        420        430        440        450
QCPICLKNYS VEHVIVDPYF NRITSKMKHC DEEVTEIEVK PDGSWRVKFK 
       460        470        480        490        500
RESERRELGE LSQWHAPDGS LCPSAVDIKR KMEMLPVKQE GYSDGPAPLK 
       510        520        530        540        550
LGIRKNRNGI WEVSKPNTNG LSSSNRQEKV GYQEKNIIPM SSSATGSGRD 
       560        570        580        590        600
GDDASVNQDA IGTFDFVANG MELDSISMNV DSGYNFPDRN QSGEGGNNEV 
       610        620        630        640        650
IVLSDSDDEN DLVITPGPAY SGCQTDGGLT FPLNPPGIIN SYNEDPHSIA 
       660        670        680        690        700
GGSSGLGLFN DDDEFDTPLW SFPSETPEAP GFQLFRSDAD VSGGLVGLHH 
       710        720        730        740        750
HSPLNCSPEI NGGYTMAPET SMASVPVVPG STGRSEANDG LVDNPLAFGR 
       760        770        780        790        800
DDPSLQIFLP TKPDASAQSG FKNQADMSNG LRSEDWISLR LGDSASGNHG 
       810        820        830        840        850
DPATTNGINS SHQMSTREGS MDTTTETASL LLGMNDSRQD KAKKQRSDNP 
       860        870        880 
FSFPRQKRSN NEQDHQTRHR SLNKICIILC AGKN

Note: No experimental confirmation available.

Length:884

Mass (Da):97,034

Last modified:August 30, 2005 - v2

Checksum:ⁱ5E44EA57FE896113

GO

Isoform 2 (identifier: Q680Q4-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
828-832: ASLLL → GLFPV
833-884: Missing.

« Hide

        10         20         30         40         50
MDLEANCKEK LSYFRIKELK DVLTQLGLSK QGKKQELVDR ILTLLSDEQA 
        60         70         80         90        100
ARLLSKKNTV AKEAVAKLVD DTYRKMQVSG ASDLASKGQV SSDTSNLKVK 
       110        120        130        140        150
GEPEDPFQPE IKVRCVCGNS LETDSMIQCE DPRCHVWQHV GCVILPDKPM 
       160        170        180        190        200
DGNPPLPESF YCEICRLTRA DPFWVTVAHP LSPVRLTATT IPNDGASTMQ 
       210        220        230        240        250
SVERTFQITR ADKDLLAKPE YDVQAWCMLL NDKVLFRMQW PQYADLQVNG 
       260        270        280        290        300
VPVRAINRPG GQLLGVNGRD DGPIITSCIR DGVNRISLSG GDVRIFCFGV 
       310        320        330        340        350
RLVKRRTLQQ VLNLIPEEGK GETFEDALAR VRRCIGGGGG DDNADSDSDI 
       360        370        380        390        400
EVVADFFGVN LRCPMSGSRI KVAGRFLPCV HMGCFDLDVF VELNQRSRKW 
       410        420        430        440        450
QCPICLKNYS VEHVIVDPYF NRITSKMKHC DEEVTEIEVK PDGSWRVKFK 
       460        470        480        490        500
RESERRELGE LSQWHAPDGS LCPSAVDIKR KMEMLPVKQE GYSDGPAPLK 
       510        520        530        540        550
LGIRKNRNGI WEVSKPNTNG LSSSNRQEKV GYQEKNIIPM SSSATGSGRD 
       560        570        580        590        600
GDDASVNQDA IGTFDFVANG MELDSISMNV DSGYNFPDRN QSGEGGNNEV 
       610        620        630        640        650
IVLSDSDDEN DLVITPGPAY SGCQTDGGLT FPLNPPGIIN SYNEDPHSIA 
       660        670        680        690        700
GGSSGLGLFN DDDEFDTPLW SFPSETPEAP GFQLFRSDAD VSGGLVGLHH 
       710        720        730        740        750
HSPLNCSPEI NGGYTMAPET SMASVPVVPG STGRSEANDG LVDNPLAFGR 
       760        770        780        790        800
DDPSLQIFLP TKPDASAQSG FKNQADMSNG LRSEDWISLR LGDSASGNHG 
       810        820        830 
DPATTNGINS SHQMSTREGS MDTTTETGLF PV

Note: No experimental confirmation available.

Show »

Length:832

Mass (Da):90,970

Checksum:ⁱ8C5DD47141D7E92F

GO

Isoform 3 (identifier: Q680Q4-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
860-873: NNEQDHQTRHRSLN → VRPRMYLSIDSDSE
874-884: Missing.

« Hide

        10         20         30         40         50
MDLEANCKEK LSYFRIKELK DVLTQLGLSK QGKKQELVDR ILTLLSDEQA 
        60         70         80         90        100
ARLLSKKNTV AKEAVAKLVD DTYRKMQVSG ASDLASKGQV SSDTSNLKVK 
       110        120        130        140        150
GEPEDPFQPE IKVRCVCGNS LETDSMIQCE DPRCHVWQHV GCVILPDKPM 
       160        170        180        190        200
DGNPPLPESF YCEICRLTRA DPFWVTVAHP LSPVRLTATT IPNDGASTMQ 
       210        220        230        240        250
SVERTFQITR ADKDLLAKPE YDVQAWCMLL NDKVLFRMQW PQYADLQVNG 
       260        270        280        290        300
VPVRAINRPG GQLLGVNGRD DGPIITSCIR DGVNRISLSG GDVRIFCFGV 
       310        320        330        340        350
RLVKRRTLQQ VLNLIPEEGK GETFEDALAR VRRCIGGGGG DDNADSDSDI 
       360        370        380        390        400
EVVADFFGVN LRCPMSGSRI KVAGRFLPCV HMGCFDLDVF VELNQRSRKW 
       410        420        430        440        450
QCPICLKNYS VEHVIVDPYF NRITSKMKHC DEEVTEIEVK PDGSWRVKFK 
       460        470        480        490        500
RESERRELGE LSQWHAPDGS LCPSAVDIKR KMEMLPVKQE GYSDGPAPLK 
       510        520        530        540        550
LGIRKNRNGI WEVSKPNTNG LSSSNRQEKV GYQEKNIIPM SSSATGSGRD 
       560        570        580        590        600
GDDASVNQDA IGTFDFVANG MELDSISMNV DSGYNFPDRN QSGEGGNNEV 
       610        620        630        640        650
IVLSDSDDEN DLVITPGPAY SGCQTDGGLT FPLNPPGIIN SYNEDPHSIA 
       660        670        680        690        700
GGSSGLGLFN DDDEFDTPLW SFPSETPEAP GFQLFRSDAD VSGGLVGLHH 
       710        720        730        740        750
HSPLNCSPEI NGGYTMAPET SMASVPVVPG STGRSEANDG LVDNPLAFGR 
       760        770        780        790        800
DDPSLQIFLP TKPDASAQSG FKNQADMSNG LRSEDWISLR LGDSASGNHG 
       810        820        830        840        850
DPATTNGINS SHQMSTREGS MDTTTETASL LLGMNDSRQD KAKKQRSDNP 
       860        870 
FSFPRQKRSV RPRMYLSIDS DSE

Show »

Length:873

Mass (Da):95,795

Checksum:ⁱ0C9BD3F7F66AA3ED

GO

Isoform 4 (identifier: Q680Q4-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
860-884: NNEQDHQTRHRSLNKICIILCAGKN → VRPRMYLSIDSDSETMNRIIRQDTGV

« Hide

        10         20         30         40         50
MDLEANCKEK LSYFRIKELK DVLTQLGLSK QGKKQELVDR ILTLLSDEQA 
        60         70         80         90        100
ARLLSKKNTV AKEAVAKLVD DTYRKMQVSG ASDLASKGQV SSDTSNLKVK 
       110        120        130        140        150
GEPEDPFQPE IKVRCVCGNS LETDSMIQCE DPRCHVWQHV GCVILPDKPM 
       160        170        180        190        200
DGNPPLPESF YCEICRLTRA DPFWVTVAHP LSPVRLTATT IPNDGASTMQ 
       210        220        230        240        250
SVERTFQITR ADKDLLAKPE YDVQAWCMLL NDKVLFRMQW PQYADLQVNG 
       260        270        280        290        300
VPVRAINRPG GQLLGVNGRD DGPIITSCIR DGVNRISLSG GDVRIFCFGV 
       310        320        330        340        350
RLVKRRTLQQ VLNLIPEEGK GETFEDALAR VRRCIGGGGG DDNADSDSDI 
       360        370        380        390        400
EVVADFFGVN LRCPMSGSRI KVAGRFLPCV HMGCFDLDVF VELNQRSRKW 
       410        420        430        440        450
QCPICLKNYS VEHVIVDPYF NRITSKMKHC DEEVTEIEVK PDGSWRVKFK 
       460        470        480        490        500
RESERRELGE LSQWHAPDGS LCPSAVDIKR KMEMLPVKQE GYSDGPAPLK 
       510        520        530        540        550
LGIRKNRNGI WEVSKPNTNG LSSSNRQEKV GYQEKNIIPM SSSATGSGRD 
       560        570        580        590        600
GDDASVNQDA IGTFDFVANG MELDSISMNV DSGYNFPDRN QSGEGGNNEV 
       610        620        630        640        650
IVLSDSDDEN DLVITPGPAY SGCQTDGGLT FPLNPPGIIN SYNEDPHSIA 
       660        670        680        690        700
GGSSGLGLFN DDDEFDTPLW SFPSETPEAP GFQLFRSDAD VSGGLVGLHH 
       710        720        730        740        750
HSPLNCSPEI NGGYTMAPET SMASVPVVPG STGRSEANDG LVDNPLAFGR 
       760        770        780        790        800
DDPSLQIFLP TKPDASAQSG FKNQADMSNG LRSEDWISLR LGDSASGNHG 
       810        820        830        840        850
DPATTNGINS SHQMSTREGS MDTTTETASL LLGMNDSRQD KAKKQRSDNP 
       860        870        880 
FSFPRQKRSV RPRMYLSIDS DSETMNRIIR QDTGV

Note: No experimental confirmation available.

Show »

Length:885

Mass (Da):97,181

Checksum:ⁱCBD103E9A048FD1D

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	335	I → S in BAD94301 (Ref. 5) Curated	1
Sequence conflictⁱ	335	I → S in BAD43867 (Ref. 5) Curated	1
Sequence conflictⁱ	436	E → D in BAD43867 (Ref. 5) Curated	1
Sequence conflictⁱ	785	D → G in BAD43867 (Ref. 5) Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_015483	828 – 832	ASLLL → GLFPV in isoform 2. 2 Publications	5
Alternative sequenceⁱVSP_015484	833 – 884	Missing in isoform 2. 2 PublicationsAdd BLAST	52
Alternative sequenceⁱVSP_015485	860 – 884	NNEQD…CAGKN → VRPRMYLSIDSDSETMNRII RQDTGV in isoform 4. 1 PublicationAdd BLAST	25
Alternative sequenceⁱVSP_015486	860 – 873	NNEQD…HRSLN → VRPRMYLSIDSDSE in isoform 3. 3 PublicationsAdd BLAST	14
Alternative sequenceⁱVSP_015487	874 – 884	Missing in isoform 3. 3 PublicationsAdd BLAST	11

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY700572 mRNA Translation: AAU00414.1 AB011483 Genomic DNA Translation: BAB08225.1 CP002688 Genomic DNA Translation: AED97324.1 CP002688 Genomic DNA Translation: AED97325.1 CP002688 Genomic DNA Translation: AED97326.1 CP002688 Genomic DNA Translation: AED97327.1 CP002688 Genomic DNA Translation: AED97328.1 CP002688 Genomic DNA Translation: ANM70389.1 AY139752 mRNA Translation: AAM98074.1 BT004542 mRNA Translation: AAO42788.1 AK175813 mRNA Translation: BAD43576.1 AK221190 mRNA Translation: BAD95283.1 AK220973 mRNA Translation: BAD94544.1 AK221249 mRNA Translation: BAD93882.1 AK221292 mRNA Translation: BAD94016.1 AK220890 mRNA Translation: BAD94301.1 AK176104 mRNA Translation: BAD43867.1 AK316983 mRNA Translation: BAH19679.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001032108.1, NM_001037031.1 [Q680Q4-2] NP_001032109.2, NM_001037032.2 [Q680Q4-1] NP_001119465.1, NM_001125993.1 [Q680Q4-4] NP_001332003.1, NM_001345415.1 [Q680Q4-3] NP_200849.2, NM_125434.4 [Q680Q4-3] NP_974969.1, NM_203240.3 [Q680Q4-4]
UniGeneⁱ	At.43746

Genome annotation databases

EnsemblPlantsⁱ	AT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3] AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4] AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2] AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1] AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4] AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3]
GeneIDⁱ	836163
Grameneⁱ	AT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3] AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4] AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2] AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1] AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4] AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3]
KEGGⁱ	ath:AT5G60410

Keywords - Coding sequence diversityⁱ

Alternative splicing

Protein	Similar proteins		Species	Score	Length	Source
Q680Q4	AT5G60410 protein (Fragment)		ARATH		723	UniRef90_Q680Q4
Q680Q4-2	Isoform 4 of E3 SUMO-protein ligase SIZ1		ARATH		885	UniRef90_Q680Q4-4
Uncharacterized protein		EUTSA		904
ATSIZ1/SIZ1		ARALL		898
Uncharacterized protein		Capsella rubella		873
E3 SUMO-protein ligase SIZ1		NOCCA		868
+11
Q680Q4-3	Isoform 4 of E3 SUMO-protein ligase SIZ1		ARATH		885	UniRef90_Q680Q4-4
Uncharacterized protein		EUTSA		904
ATSIZ1/SIZ1		ARALL		898
Uncharacterized protein		Capsella rubella		873
E3 SUMO-protein ligase SIZ1		NOCCA		868
+11
Q680Q4-4	Isoform 3 of E3 SUMO-protein ligase SIZ1		ARATH		873	UniRef90_Q680Q4-4
Uncharacterized protein		EUTSA		904
ATSIZ1/SIZ1		ARALL		898
Uncharacterized protein		Capsella rubella		873
E3 SUMO-protein ligase SIZ1		NOCCA		868
+11

Protein	Similar proteins		Species	Score	Length	Source
Q680Q4	AT5G60410 protein (Fragment)		ARATH		723	UniRef50_Q680Q4
Uncharacterized protein		Vigna angularis var. angularis		891
E3 SUMO-protein ligase SIZ1-like isoform X4		VIGRR		886
Putative chromatin regulator PHD family		ROSCH		886
SAP domain-containing protein/zf-MIZ domain-containing protein		CEPFO		877
+346

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY700572 mRNA Translation: AAU00414.1 AB011483 Genomic DNA Translation: BAB08225.1 CP002688 Genomic DNA Translation: AED97324.1 CP002688 Genomic DNA Translation: AED97325.1 CP002688 Genomic DNA Translation: AED97326.1 CP002688 Genomic DNA Translation: AED97327.1 CP002688 Genomic DNA Translation: AED97328.1 CP002688 Genomic DNA Translation: ANM70389.1 AY139752 mRNA Translation: AAM98074.1 BT004542 mRNA Translation: AAO42788.1 AK175813 mRNA Translation: BAD43576.1 AK221190 mRNA Translation: BAD95283.1 AK220973 mRNA Translation: BAD94544.1 AK221249 mRNA Translation: BAD93882.1 AK221292 mRNA Translation: BAD94016.1 AK220890 mRNA Translation: BAD94301.1 AK176104 mRNA Translation: BAD43867.1 AK316983 mRNA Translation: BAH19679.1
RefSeqⁱ	NP_001032108.1, NM_001037031.1 [Q680Q4-2] NP_001032109.2, NM_001037032.2 [Q680Q4-1] NP_001119465.1, NM_001125993.1 [Q680Q4-4] NP_001332003.1, NM_001345415.1 [Q680Q4-3] NP_200849.2, NM_125434.4 [Q680Q4-3] NP_974969.1, NM_203240.3 [Q680Q4-4]
UniGeneⁱ	At.43746

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1WEW	NMR	-	A	104-168	[»]
ProteinModelPortalⁱ	Q680Q4
SMRⁱ	Q680Q4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	21407, 17 interactors
STRINGⁱ	3702.AT5G60410.2

PTM databases

iPTMnetⁱ	Q680Q4

iPTMnetⁱ

Q680Q4

Proteomic databases

PaxDbⁱ	Q680Q4
PRIDEⁱ	Q680Q4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3] AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4] AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2] AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1] AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4] AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3]
GeneIDⁱ	836163
Grameneⁱ	AT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3] AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4] AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2] AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1] AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4] AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3]
KEGGⁱ	ath:AT5G60410

Organism-specific databases

Araportⁱ	AT5G60410
TAIRⁱ	locus:2175148 AT5G60410

Phylogenomic databases

eggNOGⁱ	KOG2169 Eukaryota ENOG410XQ2E LUCA
HOGENOMⁱ	HOG000029457
InParanoidⁱ	Q680Q4
OMAⁱ	EHDSISM
PhylomeDBⁱ	Q680Q4

Enzyme and pathway databases

UniPathwayⁱ	UPA00886
Reactomeⁱ	R-ATH-3108214 SUMOylation of DNA damage response and repair proteins R-ATH-3899300 SUMOylation of transcription cofactors R-ATH-4085377 SUMOylation of SUMOylation proteins R-ATH-4615885 SUMOylation of DNA replication proteins R-ATH-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-ATH-5696395 Formation of Incision Complex in GG-NER

Miscellaneous databases

EvolutionaryTraceⁱ	Q680Q4
Protein Ontology More...PROⁱ	PR:Q680Q4

Gene expression databases

ExpressionAtlasⁱ	Q680Q4 baseline and differential
Genevisibleⁱ	Q680Q4 AT

Family and domain databases

Gene3Dⁱ	1.10.720.30, 1 hit 3.30.40.10, 2 hits
InterProⁱ	View protein in InterPro IPR003034 SAP_dom IPR036361 SAP_dom_sf IPR031141 SIZ1_plant IPR019786 Zinc_finger_PHD-type_CS IPR011011 Znf_FYVE_PHD IPR004181 Znf_MIZ IPR001965 Znf_PHD IPR013083 Znf_RING/FYVE/PHD
PANTHERⁱ	PTHR10782:SF34 PTHR10782:SF34, 1 hit
Pfamⁱ	View protein in Pfam PF02037 SAP, 1 hit PF02891 zf-MIZ, 1 hit
SMARTⁱ	View protein in SMART SM00249 PHD, 1 hit SM00513 SAP, 1 hit
SUPFAMⁱ	SSF57903 SSF57903, 1 hit SSF68906 SSF68906, 1 hit
PROSITEⁱ	View protein in PROSITE PS50800 SAP, 1 hit PS01359 ZF_PHD_1, 1 hit PS51044 ZF_SP_RING, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SIZ1_ARATH
Accessionⁱ	Q680Q4Primary (citable) accession number: Q680Q4 Secondary accession number(s): B9DG12 , Q56YS2, Q56ZS1, Q67ZL3, Q9FKK4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
	Last sequence update:	August 30, 2005
	Last modified:	November 7, 2018
	This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

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UniProtKB - Q680Q4 (SIZ1_ARATH)

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E3 SUMO-protein ligase SIZ1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein sumoylation

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

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<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

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Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

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<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

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Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

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Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

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Sequence databases

3D structure databases

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PTM databases

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Protocols and materials databases

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Functionⁱ

Pathwayⁱ: protein sumoylation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ