UniProtKB - Q680Q4 (SIZ1_ARATH)
Protein
E3 SUMO-protein ligase SIZ1
Gene
SIZ1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
E3 SUMO protein ligase involved in regulation processes. Mediates SUMO/ attachment to PHR1, a MYB transcriptional activator controlling the phosphate deficiency responses (PubMed:15894620). Functions as an upstream negative regulator of salicylic acid (SA) accumulation and subsequent SA-mediated systemic acquired resistance (SAR) signaling. Probably not involved in jasmonic acid (JA)-mediated defense response. Participates in abiotic stress-induced sumoylation. Controls heat shock-induced SUMO1 and SUMO2 conjugation and facilitates basal thermotolerance. Involved in freezing tolerance by mediating sumoylation of ICE1, a transcription activator of the cold signaling regulator CBF3/DREB1A. Acts as positive regulator of drought stress tolerance. Acts as floral repressor that promotes FLC expression by repressing FLD activity through sumoylation. Acts as negative regulator of abscisic acid (ABA) signaling through ABI5 sumoylation. Mediates sumoylation of SCE1, GTE3 and GTE5. Functions as negative regulator of SnRK1 signaling through sumoylation of several components of the SnRK1 complex (PubMed:26662259).10 Publications
: protein sumoylation Pathwayi
This protein is involved in the pathway protein sumoylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 112 – 168 | PHD-typeAdd BLAST | 57 | |
Zinc fingeri | 348 – 425 | SP-RING-typePROSITE-ProRule annotationAdd BLAST | 78 |
GO - Molecular functioni
- SUMO transferase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- cell division Source: TAIR
- cellular response to phosphate starvation Source: TAIR
- defense response Source: UniProtKB-KW
- detection of phosphate ion Source: TAIR
- developmental growth Source: UniProtKB
- embryo sac development Source: TAIR
- heat acclimation Source: UniProtKB
- negative regulation of flower development Source: TAIR
- negative regulation of systemic acquired resistance Source: UniProtKB
- plant ovule development Source: TAIR
- pollen tube guidance Source: TAIR
- protein sumoylation Source: UniProtKB
- regulation of abscisic acid-activated signaling pathway Source: TAIR
- regulation of growth Source: TAIR
- regulation of nitrate assimilation Source: TAIR
- regulation of response to water deprivation Source: TAIR
- regulation of salicylic acid metabolic process Source: TAIR
- response to freezing Source: TAIR
- response to water deprivation Source: UniProtKB
- unidimensional cell growth Source: TAIR
Keywordsi
Molecular function | Transferase |
Biological process | Flowering, Plant defense, Stress response, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00886 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 SUMO-protein ligase SIZ11 Publication (EC:2.3.2.-Curated)Alternative name(s): E3 SUMO-protein transferase SIZ1Curated |
Gene namesi | Name:SIZ11 Publication Ordered Locus Names:At5g60410Imported ORF Names:MUF9.5Imported, MUF9.70 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G60410 |
TAIRi | locus:2175148, AT5G60410 |
Subcellular locationi
Nucleus
- Nucleus speckle 1 Publication
Nucleus
- nuclear speck Source: UniProtKB-SubCell
- nucleus Source: TAIR
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Dwarf phenotype. Heat-sensitive phenotype. Early flowering under short day. Elevated level of salicylic acid (SA), increased expression of pathogenesis-related (PR) genes and increased resistance to the bacterial pathogen P.syringae. ABA hypersensitivity during seed germination primary root growth. Exaggerated prototypical Pi-starvation responses, including increased root-shoot mass ratio and greater anthocyanin accumulation (PubMed:15894620).7 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 100 | K → R: Strongly reduces autosumoylation; when associated with R-488. 1 Publication | 1 | |
Mutagenesisi | 379 | C → A: Abolishes activity. 1 Publication | 1 | |
Mutagenesisi | 488 | K → R: Strongly reduces autosumoylation; when associated with R-100. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218984 | 1 – 884 | E3 SUMO-protein ligase SIZ1Add BLAST | 884 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 100 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) | ||
Cross-linki | 488 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) |
Post-translational modificationi
Autosumoylated at Lys-100 and Lys-488.
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | Q680Q4 |
PRIDEi | Q680Q4 |
ProteomicsDBi | 232620 [Q680Q4-1] |
PTM databases
iPTMneti | Q680Q4 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
ExpressionAtlasi | Q680Q4, baseline and differential |
Genevisiblei | Q680Q4, AT |
Interactioni
Subunit structurei
Interacts (via PHD domain) with SCE1, GTE3 and GTE5.
1 PublicationProtein-protein interaction databases
BioGRIDi | 21407, 18 interactors |
STRINGi | 3702.AT5G60410.2 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q680Q4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q680Q4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 45 | SAPPROSITE-ProRule annotationAdd BLAST | 35 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 336 – 340 | Poly-Gly | 5 |
Domaini
The PHD-type zinc finger mediates interaction with SCE1, GTE3 and GTE5 and is required for E3 activity.1 Publication
Sequence similaritiesi
Belongs to the PIAS family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 112 – 168 | PHD-typeAdd BLAST | 57 | |
Zinc fingeri | 348 – 425 | SP-RING-typePROSITE-ProRule annotationAdd BLAST | 78 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2169, Eukaryota |
InParanoidi | Q680Q4 |
OMAi | WEVRRPN |
OrthoDBi | 141085at2759 |
PhylomeDBi | Q680Q4 |
Family and domain databases
Gene3Di | 1.10.720.30, 1 hit 3.30.40.10, 2 hits |
InterProi | View protein in InterPro IPR003034, SAP_dom IPR036361, SAP_dom_sf IPR031141, SIZ1_plant IPR019786, Zinc_finger_PHD-type_CS IPR011011, Znf_FYVE_PHD IPR004181, Znf_MIZ IPR001965, Znf_PHD IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR10782:SF73, PTHR10782:SF73, 1 hit |
Pfami | View protein in Pfam PF02037, SAP, 1 hit PF02891, zf-MIZ, 1 hit |
SMARTi | View protein in SMART SM00249, PHD, 1 hit SM00513, SAP, 1 hit |
SUPFAMi | SSF57903, SSF57903, 1 hit SSF68906, SSF68906, 1 hit |
PROSITEi | View protein in PROSITE PS50800, SAP, 1 hit PS01359, ZF_PHD_1, 1 hit PS51044, ZF_SP_RING, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q680Q4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDLEANCKEK LSYFRIKELK DVLTQLGLSK QGKKQELVDR ILTLLSDEQA
60 70 80 90 100
ARLLSKKNTV AKEAVAKLVD DTYRKMQVSG ASDLASKGQV SSDTSNLKVK
110 120 130 140 150
GEPEDPFQPE IKVRCVCGNS LETDSMIQCE DPRCHVWQHV GCVILPDKPM
160 170 180 190 200
DGNPPLPESF YCEICRLTRA DPFWVTVAHP LSPVRLTATT IPNDGASTMQ
210 220 230 240 250
SVERTFQITR ADKDLLAKPE YDVQAWCMLL NDKVLFRMQW PQYADLQVNG
260 270 280 290 300
VPVRAINRPG GQLLGVNGRD DGPIITSCIR DGVNRISLSG GDVRIFCFGV
310 320 330 340 350
RLVKRRTLQQ VLNLIPEEGK GETFEDALAR VRRCIGGGGG DDNADSDSDI
360 370 380 390 400
EVVADFFGVN LRCPMSGSRI KVAGRFLPCV HMGCFDLDVF VELNQRSRKW
410 420 430 440 450
QCPICLKNYS VEHVIVDPYF NRITSKMKHC DEEVTEIEVK PDGSWRVKFK
460 470 480 490 500
RESERRELGE LSQWHAPDGS LCPSAVDIKR KMEMLPVKQE GYSDGPAPLK
510 520 530 540 550
LGIRKNRNGI WEVSKPNTNG LSSSNRQEKV GYQEKNIIPM SSSATGSGRD
560 570 580 590 600
GDDASVNQDA IGTFDFVANG MELDSISMNV DSGYNFPDRN QSGEGGNNEV
610 620 630 640 650
IVLSDSDDEN DLVITPGPAY SGCQTDGGLT FPLNPPGIIN SYNEDPHSIA
660 670 680 690 700
GGSSGLGLFN DDDEFDTPLW SFPSETPEAP GFQLFRSDAD VSGGLVGLHH
710 720 730 740 750
HSPLNCSPEI NGGYTMAPET SMASVPVVPG STGRSEANDG LVDNPLAFGR
760 770 780 790 800
DDPSLQIFLP TKPDASAQSG FKNQADMSNG LRSEDWISLR LGDSASGNHG
810 820 830 840 850
DPATTNGINS SHQMSTREGS MDTTTETASL LLGMNDSRQD KAKKQRSDNP
860 870 880
FSFPRQKRSN NEQDHQTRHR SLNKICIILC AGKN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 335 | I → S in BAD94301 (Ref. 5) Curated | 1 | |
Sequence conflicti | 335 | I → S in BAD43867 (Ref. 5) Curated | 1 | |
Sequence conflicti | 436 | E → D in BAD43867 (Ref. 5) Curated | 1 | |
Sequence conflicti | 785 | D → G in BAD43867 (Ref. 5) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015483 | 828 – 832 | ASLLL → GLFPV in isoform 2. 2 Publications | 5 | |
Alternative sequenceiVSP_015484 | 833 – 884 | Missing in isoform 2. 2 PublicationsAdd BLAST | 52 | |
Alternative sequenceiVSP_015485 | 860 – 884 | NNEQD…CAGKN → VRPRMYLSIDSDSETMNRII RQDTGV in isoform 4. 1 PublicationAdd BLAST | 25 | |
Alternative sequenceiVSP_015486 | 860 – 873 | NNEQD…HRSLN → VRPRMYLSIDSDSE in isoform 3. 3 PublicationsAdd BLAST | 14 | |
Alternative sequenceiVSP_015487 | 874 – 884 | Missing in isoform 3. 3 PublicationsAdd BLAST | 11 |
Sequence databases
Genome annotation databases
EnsemblPlantsi | AT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3] AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4] AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2] AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1] AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4] AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3] |
GeneIDi | 836163 |
Gramenei | AT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3] AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4] AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2] AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1] AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4] AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3] |
KEGGi | ath:AT5G60410 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WEW | NMR | - | A | 104-168 | [»] | |
SMRi | Q680Q4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 21407, 18 interactors |
STRINGi | 3702.AT5G60410.2 |
PTM databases
iPTMneti | Q680Q4 |
Proteomic databases
PaxDbi | Q680Q4 |
PRIDEi | Q680Q4 |
ProteomicsDBi | 232620 [Q680Q4-1] |
Genome annotation databases
EnsemblPlantsi | AT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3] AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4] AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2] AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1] AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4] AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3] |
GeneIDi | 836163 |
Gramenei | AT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3] AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4] AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2] AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1] AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4] AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3] |
KEGGi | ath:AT5G60410 |
Organism-specific databases
Araporti | AT5G60410 |
TAIRi | locus:2175148, AT5G60410 |
Phylogenomic databases
eggNOGi | KOG2169, Eukaryota |
InParanoidi | Q680Q4 |
OMAi | WEVRRPN |
OrthoDBi | 141085at2759 |
PhylomeDBi | Q680Q4 |
Enzyme and pathway databases
UniPathwayi | UPA00886 |
Miscellaneous databases
EvolutionaryTracei | Q680Q4 |
PROi | PR:Q680Q4 |
Gene expression databases
ExpressionAtlasi | Q680Q4, baseline and differential |
Genevisiblei | Q680Q4, AT |
Family and domain databases
Gene3Di | 1.10.720.30, 1 hit 3.30.40.10, 2 hits |
InterProi | View protein in InterPro IPR003034, SAP_dom IPR036361, SAP_dom_sf IPR031141, SIZ1_plant IPR019786, Zinc_finger_PHD-type_CS IPR011011, Znf_FYVE_PHD IPR004181, Znf_MIZ IPR001965, Znf_PHD IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR10782:SF73, PTHR10782:SF73, 1 hit |
Pfami | View protein in Pfam PF02037, SAP, 1 hit PF02891, zf-MIZ, 1 hit |
SMARTi | View protein in SMART SM00249, PHD, 1 hit SM00513, SAP, 1 hit |
SUPFAMi | SSF57903, SSF57903, 1 hit SSF68906, SSF68906, 1 hit |
PROSITEi | View protein in PROSITE PS50800, SAP, 1 hit PS01359, ZF_PHD_1, 1 hit PS51044, ZF_SP_RING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SIZ1_ARATH | |
Accessioni | Q680Q4Primary (citable) accession number: Q680Q4 Secondary accession number(s): B9DG12 Q9FKK4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2005 |
Last sequence update: | August 30, 2005 | |
Last modified: | August 12, 2020 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families