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Protein

E3 SUMO-protein ligase SIZ1

Gene

SIZ1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 SUMO protein ligase involved in regulation processes. Mediates SUMO/ attachment to PHR1, a MYB transcriptional activator controlling the phosphate deficiency responses (PubMed:15894620). Functions as an upstream negative regulator of salicylic acid (SA) accumulation and subsequent SA-mediated systemic acquired resistance (SAR) signaling. Probably not involved in jasmonic acid (JA)-mediated defense response. Participates in abiotic stress-induced sumoylation. Controls heat shock-induced SUMO1 and SUMO2 conjugation and facilitates basal thermotolerance. Involved in freezing tolerance by mediating sumoylation of ICE1, a transcription activator of the cold signaling regulator CBF3/DREB1A. Acts as positive regulator of drought stress tolerance. Acts as floral repressor that promotes FLC expression by repressing FLD activity through sumoylation. Acts as negative regulator of abscisic acid (ABA) signaling through ABI5 sumoylation. Mediates sumoylation of SCE1, GTE3 and GTE5. Functions as negative regulator of SnRK1 signaling through sumoylation of several components of the SnRK1 complex (PubMed:26662259).10 Publications

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri112 – 168PHD-typeAdd BLAST57
Zinc fingeri348 – 425SP-RING-typePROSITE-ProRule annotationAdd BLAST78

GO - Molecular functioni

  • SUMO transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processFlowering, Plant defense, Stress response, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-3108214 SUMOylation of DNA damage response and repair proteins
R-ATH-3899300 SUMOylation of transcription cofactors
R-ATH-4085377 SUMOylation of SUMOylation proteins
R-ATH-4615885 SUMOylation of DNA replication proteins
R-ATH-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-ATH-5696395 Formation of Incision Complex in GG-NER
UniPathwayi
UPA00886

Names & Taxonomyi

Protein namesi
Recommended name:
E3 SUMO-protein ligase SIZ11 Publication (EC:2.3.2.-Curated)
Alternative name(s):
E3 SUMO-protein transferase SIZ1Curated
Gene namesi
Name:SIZ11 Publication
Ordered Locus Names:At5g60410Imported
ORF Names:MUF9.5Imported, MUF9.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G60410
TAIRilocus:2175148 AT5G60410

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Dwarf phenotype. Heat-sensitive phenotype. Early flowering under short day. Elevated level of salicylic acid (SA), increased expression of pathogenesis-related (PR) genes and increased resistance to the bacterial pathogen P.syringae. ABA hypersensitivity during seed germination primary root growth. Exaggerated prototypical Pi-starvation responses, including increased root-shoot mass ratio and greater anthocyanin accumulation (PubMed:15894620).7 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi100K → R: Strongly reduces autosumoylation; when associated with R-488. 1 Publication1
Mutagenesisi379C → A: Abolishes activity. 1 Publication1
Mutagenesisi488K → R: Strongly reduces autosumoylation; when associated with R-100. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002189841 – 884E3 SUMO-protein ligase SIZ1Add BLAST884

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki100Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki488Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modificationi

Autosumoylated at Lys-100 and Lys-488.

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ680Q4
PRIDEiQ680Q4

PTM databases

iPTMnetiQ680Q4

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

ExpressionAtlasiQ680Q4 baseline and differential
GenevisibleiQ680Q4 AT

Interactioni

Subunit structurei

Interacts (via PHD domain) with SCE1, GTE3 and GTE5.1 Publication

Protein-protein interaction databases

BioGridi21407, 17 interactors
STRINGi3702.AT5G60410.2

Structurei

Secondary structure

1884
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ680Q4
SMRiQ680Q4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ680Q4

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 45SAPPROSITE-ProRule annotationAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi336 – 340Poly-Gly5

Domaini

The PHD-type zinc finger mediates interaction with SCE1, GTE3 and GTE5 and is required for E3 activity.1 Publication

Sequence similaritiesi

Belongs to the PIAS family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri112 – 168PHD-typeAdd BLAST57
Zinc fingeri348 – 425SP-RING-typePROSITE-ProRule annotationAdd BLAST78

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2169 Eukaryota
ENOG410XQ2E LUCA
HOGENOMiHOG000029457
InParanoidiQ680Q4
OMAiEHDSISM
PhylomeDBiQ680Q4

Family and domain databases

Gene3Di1.10.720.30, 1 hit
3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
IPR031141 SIZ1_plant
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR004181 Znf_MIZ
IPR001965 Znf_PHD
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR10782:SF34 PTHR10782:SF34, 1 hit
PfamiView protein in Pfam
PF02037 SAP, 1 hit
PF02891 zf-MIZ, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 1 hit
SM00513 SAP, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
SSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS50800 SAP, 1 hit
PS01359 ZF_PHD_1, 1 hit
PS51044 ZF_SP_RING, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q680Q4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDLEANCKEK LSYFRIKELK DVLTQLGLSK QGKKQELVDR ILTLLSDEQA
60 70 80 90 100
ARLLSKKNTV AKEAVAKLVD DTYRKMQVSG ASDLASKGQV SSDTSNLKVK
110 120 130 140 150
GEPEDPFQPE IKVRCVCGNS LETDSMIQCE DPRCHVWQHV GCVILPDKPM
160 170 180 190 200
DGNPPLPESF YCEICRLTRA DPFWVTVAHP LSPVRLTATT IPNDGASTMQ
210 220 230 240 250
SVERTFQITR ADKDLLAKPE YDVQAWCMLL NDKVLFRMQW PQYADLQVNG
260 270 280 290 300
VPVRAINRPG GQLLGVNGRD DGPIITSCIR DGVNRISLSG GDVRIFCFGV
310 320 330 340 350
RLVKRRTLQQ VLNLIPEEGK GETFEDALAR VRRCIGGGGG DDNADSDSDI
360 370 380 390 400
EVVADFFGVN LRCPMSGSRI KVAGRFLPCV HMGCFDLDVF VELNQRSRKW
410 420 430 440 450
QCPICLKNYS VEHVIVDPYF NRITSKMKHC DEEVTEIEVK PDGSWRVKFK
460 470 480 490 500
RESERRELGE LSQWHAPDGS LCPSAVDIKR KMEMLPVKQE GYSDGPAPLK
510 520 530 540 550
LGIRKNRNGI WEVSKPNTNG LSSSNRQEKV GYQEKNIIPM SSSATGSGRD
560 570 580 590 600
GDDASVNQDA IGTFDFVANG MELDSISMNV DSGYNFPDRN QSGEGGNNEV
610 620 630 640 650
IVLSDSDDEN DLVITPGPAY SGCQTDGGLT FPLNPPGIIN SYNEDPHSIA
660 670 680 690 700
GGSSGLGLFN DDDEFDTPLW SFPSETPEAP GFQLFRSDAD VSGGLVGLHH
710 720 730 740 750
HSPLNCSPEI NGGYTMAPET SMASVPVVPG STGRSEANDG LVDNPLAFGR
760 770 780 790 800
DDPSLQIFLP TKPDASAQSG FKNQADMSNG LRSEDWISLR LGDSASGNHG
810 820 830 840 850
DPATTNGINS SHQMSTREGS MDTTTETASL LLGMNDSRQD KAKKQRSDNP
860 870 880
FSFPRQKRSN NEQDHQTRHR SLNKICIILC AGKN
Note: No experimental confirmation available.
Length:884
Mass (Da):97,034
Last modified:August 30, 2005 - v2
Checksum:i5E44EA57FE896113
GO
Isoform 2 (identifier: Q680Q4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     828-832: ASLLL → GLFPV
     833-884: Missing.

Note: No experimental confirmation available.
Show »
Length:832
Mass (Da):90,970
Checksum:i8C5DD47141D7E92F
GO
Isoform 3 (identifier: Q680Q4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     860-873: NNEQDHQTRHRSLN → VRPRMYLSIDSDSE
     874-884: Missing.

Show »
Length:873
Mass (Da):95,795
Checksum:i0C9BD3F7F66AA3ED
GO
Isoform 4 (identifier: Q680Q4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     860-884: NNEQDHQTRHRSLNKICIILCAGKN → VRPRMYLSIDSDSETMNRIIRQDTGV

Note: No experimental confirmation available.
Show »
Length:885
Mass (Da):97,181
Checksum:iCBD103E9A048FD1D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti335I → S in BAD94301 (Ref. 5) Curated1
Sequence conflicti335I → S in BAD43867 (Ref. 5) Curated1
Sequence conflicti436E → D in BAD43867 (Ref. 5) Curated1
Sequence conflicti785D → G in BAD43867 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015483828 – 832ASLLL → GLFPV in isoform 2. 2 Publications5
Alternative sequenceiVSP_015484833 – 884Missing in isoform 2. 2 PublicationsAdd BLAST52
Alternative sequenceiVSP_015485860 – 884NNEQD…CAGKN → VRPRMYLSIDSDSETMNRII RQDTGV in isoform 4. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_015486860 – 873NNEQD…HRSLN → VRPRMYLSIDSDSE in isoform 3. 3 PublicationsAdd BLAST14
Alternative sequenceiVSP_015487874 – 884Missing in isoform 3. 3 PublicationsAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY700572 mRNA Translation: AAU00414.1
AB011483 Genomic DNA Translation: BAB08225.1
CP002688 Genomic DNA Translation: AED97324.1
CP002688 Genomic DNA Translation: AED97325.1
CP002688 Genomic DNA Translation: AED97326.1
CP002688 Genomic DNA Translation: AED97327.1
CP002688 Genomic DNA Translation: AED97328.1
CP002688 Genomic DNA Translation: ANM70389.1
AY139752 mRNA Translation: AAM98074.1
BT004542 mRNA Translation: AAO42788.1
AK175813 mRNA Translation: BAD43576.1
AK221190 mRNA Translation: BAD95283.1
AK220973 mRNA Translation: BAD94544.1
AK221249 mRNA Translation: BAD93882.1
AK221292 mRNA Translation: BAD94016.1
AK220890 mRNA Translation: BAD94301.1
AK176104 mRNA Translation: BAD43867.1
AK316983 mRNA Translation: BAH19679.1
RefSeqiNP_001032108.1, NM_001037031.1 [Q680Q4-2]
NP_001032109.2, NM_001037032.2 [Q680Q4-1]
NP_001119465.1, NM_001125993.1 [Q680Q4-4]
NP_001332003.1, NM_001345415.1 [Q680Q4-3]
NP_200849.2, NM_125434.4 [Q680Q4-3]
NP_974969.1, NM_203240.3 [Q680Q4-4]
UniGeneiAt.43746

Genome annotation databases

EnsemblPlantsiAT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3]
AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4]
AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2]
AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1]
AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4]
AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3]
GeneIDi836163
GrameneiAT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3]
AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4]
AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2]
AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1]
AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4]
AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3]
KEGGiath:AT5G60410

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY700572 mRNA Translation: AAU00414.1
AB011483 Genomic DNA Translation: BAB08225.1
CP002688 Genomic DNA Translation: AED97324.1
CP002688 Genomic DNA Translation: AED97325.1
CP002688 Genomic DNA Translation: AED97326.1
CP002688 Genomic DNA Translation: AED97327.1
CP002688 Genomic DNA Translation: AED97328.1
CP002688 Genomic DNA Translation: ANM70389.1
AY139752 mRNA Translation: AAM98074.1
BT004542 mRNA Translation: AAO42788.1
AK175813 mRNA Translation: BAD43576.1
AK221190 mRNA Translation: BAD95283.1
AK220973 mRNA Translation: BAD94544.1
AK221249 mRNA Translation: BAD93882.1
AK221292 mRNA Translation: BAD94016.1
AK220890 mRNA Translation: BAD94301.1
AK176104 mRNA Translation: BAD43867.1
AK316983 mRNA Translation: BAH19679.1
RefSeqiNP_001032108.1, NM_001037031.1 [Q680Q4-2]
NP_001032109.2, NM_001037032.2 [Q680Q4-1]
NP_001119465.1, NM_001125993.1 [Q680Q4-4]
NP_001332003.1, NM_001345415.1 [Q680Q4-3]
NP_200849.2, NM_125434.4 [Q680Q4-3]
NP_974969.1, NM_203240.3 [Q680Q4-4]
UniGeneiAt.43746

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WEWNMR-A104-168[»]
ProteinModelPortaliQ680Q4
SMRiQ680Q4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21407, 17 interactors
STRINGi3702.AT5G60410.2

PTM databases

iPTMnetiQ680Q4

Proteomic databases

PaxDbiQ680Q4
PRIDEiQ680Q4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3]
AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4]
AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2]
AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1]
AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4]
AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3]
GeneIDi836163
GrameneiAT5G60410.1; AT5G60410.1; AT5G60410 [Q680Q4-3]
AT5G60410.2; AT5G60410.2; AT5G60410 [Q680Q4-4]
AT5G60410.3; AT5G60410.3; AT5G60410 [Q680Q4-2]
AT5G60410.4; AT5G60410.4; AT5G60410 [Q680Q4-1]
AT5G60410.5; AT5G60410.5; AT5G60410 [Q680Q4-4]
AT5G60410.6; AT5G60410.6; AT5G60410 [Q680Q4-3]
KEGGiath:AT5G60410

Organism-specific databases

AraportiAT5G60410
TAIRilocus:2175148 AT5G60410

Phylogenomic databases

eggNOGiKOG2169 Eukaryota
ENOG410XQ2E LUCA
HOGENOMiHOG000029457
InParanoidiQ680Q4
OMAiEHDSISM
PhylomeDBiQ680Q4

Enzyme and pathway databases

UniPathwayi
UPA00886

ReactomeiR-ATH-3108214 SUMOylation of DNA damage response and repair proteins
R-ATH-3899300 SUMOylation of transcription cofactors
R-ATH-4085377 SUMOylation of SUMOylation proteins
R-ATH-4615885 SUMOylation of DNA replication proteins
R-ATH-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-ATH-5696395 Formation of Incision Complex in GG-NER

Miscellaneous databases

EvolutionaryTraceiQ680Q4
PROiPR:Q680Q4

Gene expression databases

ExpressionAtlasiQ680Q4 baseline and differential
GenevisibleiQ680Q4 AT

Family and domain databases

Gene3Di1.10.720.30, 1 hit
3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
IPR031141 SIZ1_plant
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR004181 Znf_MIZ
IPR001965 Znf_PHD
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR10782:SF34 PTHR10782:SF34, 1 hit
PfamiView protein in Pfam
PF02037 SAP, 1 hit
PF02891 zf-MIZ, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 1 hit
SM00513 SAP, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
SSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS50800 SAP, 1 hit
PS01359 ZF_PHD_1, 1 hit
PS51044 ZF_SP_RING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSIZ1_ARATH
AccessioniPrimary (citable) accession number: Q680Q4
Secondary accession number(s): B9DG12
, Q56YS2, Q56ZS1, Q67ZL3, Q9FKK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: November 7, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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