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Protein

Inorganic pyrophosphatase 1

Gene

PS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the specific cleavage of pyrophosphate.1 Publication

Catalytic activityi

Diphosphate + H2O = 2 phosphate.1 Publication

Cofactori

Mg2+1 Publication, Fe2+1 Publication, Ni2+1 Publication, Co2+1 Publication, Mn2+1 PublicationNote: Magnesium. Can also use iron, nickel, cobalt and manganese, but not zinc ions.1 Publication

Kineticsi

  1. KM=38.8 µM for pyrophosphate PPi (at 37 degrees Celsius)1 Publication
  1. Vmax=0.3 nmol/min/µg enzyme with PPi as substrate (at 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 8-8.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei19NucleophileCurated1
Metal bindingi19MagnesiumBy similarity1
Active sitei21Proton donorBy similarity1
Metal bindingi21MagnesiumBy similarity1
Binding sitei30SubstrateBy similarity1
Binding sitei105SubstrateBy similarity1
Metal bindingi190MagnesiumBy similarity1

GO - Molecular functioni

  • inorganic diphosphatase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • phosphatase activity Source: GO_Central
  • pyrophosphatase activity Source: TAIR

GO - Biological processi

  • cellular response to phosphate starvation Source: UniProtKB
  • protein tetramerization Source: UniProtKB

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G73010-MONOMER
BRENDAi3.6.1.1 399
ReactomeiR-ATH-1483191 Synthesis of PC
R-ATH-1483213 Synthesis of PE

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic pyrophosphatase 1 (EC:3.6.1.1)
Short name:
AtPPsPase1
Short name:
PPi phosphatase 1
Short name:
Pyrophosphate-specific phosphatase 1
Alternative name(s):
Protein PHOSPHATE STARVATION-INDUCED GENE 2
Short name:
AtPS2
Gene namesi
Name:PS2
Ordered Locus Names:At1g73010
ORF Names:F3N23.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G73010
TAIRilocus:2032748 AT1G73010

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19D → H: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004042561 – 295Inorganic pyrophosphatase 1Add BLAST295

Proteomic databases

PaxDbiQ67YC0
PRIDEiQ67YC0

Expressioni

Inductioni

Strongly induced upon phosphate (Pi) starvation.2 Publications

Gene expression databases

ExpressionAtlasiQ67YC0 baseline and differential
GenevisibleiQ67YC0 AT

Interactioni

Subunit structurei

Tetramer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G73010.1

Structurei

3D structure databases

ProteinModelPortaliQ67YC0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 13Poly-Asn10

Sequence similaritiesi

Belongs to the HAD-like hydrolase superfamily.Curated

Phylogenomic databases

eggNOGiKOG3120 Eukaryota
ENOG4111NAQ LUCA
HOGENOMiHOG000238030
InParanoidiQ67YC0
KOiK13248
OMAiSPNMCKG
OrthoDBiEOG09360K2I
PhylomeDBiQ67YC0

Family and domain databases

Gene3Di3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR016965 Pase_PHOSPHO-typ
IPR006384 PyrdxlP_Pase-rel
PANTHERiPTHR20889 PTHR20889, 1 hit
PfamiView protein in Pfam
PF06888 Put_Phosphatase, 1 hit
PIRSFiPIRSF031051 PyrdxlP_Pase_PHOSPHO2, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01489 DKMTPPase-SF, 1 hit

Sequencei

Sequence statusi: Complete.

Q67YC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYNSNSNNN NNNIVVVFDF DKTIIDVDSD NWVIDELGFT DLFNQLLPTM
60 70 80 90 100
PWNTLMDRMM KELHDQGKTI EEIKQVLRTI PIHPRVVPAI KSAHDLGCEL
110 120 130 140 150
RIVSDANMFF IETIVEHLGI SELFSEINSN PGYVDERGTL KISPYHDFTK
160 170 180 190 200
SPHSCSCGTC PPNMCKGLII ERIQQSLAKE GKKKMIYLGD GAGDYCPSLK
210 220 230 240 250
LNTEDYVMPR KNFPVWDLIS QNPMLIKAAI REWTDGQSME MILIGTIEEI
260 270 280 290
RLEEEKEKML TSAENNCKMQ TISIGINNVH HEPILPRALR VSQSS
Length:295
Mass (Da):33,515
Last modified:October 11, 2004 - v1
Checksum:i3B353D382597E74E
GO

Sequence cautioni

The sequence AAD55648 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7 – 8Missing in AAM63155 (Ref. 5) Curated2
Sequence conflicti229A → V in AAM63155 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC008017 Genomic DNA Translation: AAD55648.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE35403.1
AK176548 mRNA Translation: BAD44311.1
BT030334 mRNA Translation: ABO38747.1
AY085944 mRNA Translation: AAM63155.1
PIRiE96755
RefSeqiNP_565052.1, NM_105959.3
UniGeneiAt.11680

Genome annotation databases

EnsemblPlantsiAT1G73010.1; AT1G73010.1; AT1G73010
GeneIDi843632
GrameneiAT1G73010.1; AT1G73010.1; AT1G73010
KEGGiath:AT1G73010

Similar proteinsi

Entry informationi

Entry nameiPPSP1_ARATH
AccessioniPrimary (citable) accession number: Q67YC0
Secondary accession number(s): Q8LDK9, Q9SSM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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