UniProtKB - Q66JT0 (WEE2_MOUSE)
Wee1-like protein kinase 2
Wee2
Functioni
Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.
3 PublicationsCatalytic activityi
- EC:2.7.10.2PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 237 | ATPCurated | 1 | |
Active sitei | 334 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 339 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 375 | Magnesium; via carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 214 – 222 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- magnesium ion binding Source: InterPro
- non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
- protein kinase activity Source: GO_Central
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein tyrosine kinase activity Source: GO_Central
GO - Biological processi
- female meiotic nuclear division Source: UniProtKB
- female pronucleus assembly Source: UniProtKB
- mitotic cell cycle Source: InterPro
- negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
- negative regulation of oocyte maturation Source: UniProtKB
- positive regulation of phosphorylation Source: UniProtKB
- regulation of fertilization Source: UniProtKB
- regulation of meiosis I Source: UniProtKB
Keywordsi
Molecular function | Kinase, Transferase, Tyrosine-protein kinase |
Biological process | Meiosis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Wee1-like protein kinase 2 (EC:2.7.10.2)Alternative name(s): Wee1-like protein kinase 1B Wee1B kinase Short name: mWee1B |
Gene namesi | Name:Wee2 Synonyms:Wee1b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3027899, Wee2 |
VEuPathDBi | HostDB:ENSMUSG00000037159 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 15 | S → A: Abolishes phosphorylation and impairs ability to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage and metaphase II exit during egg activation. 2 Publications | 1 | |
Mutagenesisi | 15 | S → D: Mimicks phosphorylation state, lesading to enhance kinase activity and promote pronuclear formation. 2 Publications | 1 | |
Mutagenesisi | 167 – 169 | Missing : Abolishes nuclear localization. 1 Publication | 3 | |
Mutagenesisi | 237 | K → M: Loss of function. 2 Publications | 1 | |
Mutagenesisi | 311 | L → A: Abolishes the cytoplasmic localization; when associated with A-316; A-320 and A-322. 1 Publication | 1 | |
Mutagenesisi | 316 | L → A: Abolishes the cytoplasmic localization; when associated with A-311; A-320 and A-322. 1 Publication | 1 | |
Mutagenesisi | 320 | L → A: Abolishes the cytoplasmic localization; when associated with A-311; A-316 and A-322. 1 Publication | 1 | |
Mutagenesisi | 322 | L → A: Abolishes the cytoplasmic localization; when associated with A-311; A-316 and A-320. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000248080 | 1 – 555 | Wee1-like protein kinase 2Add BLAST | 555 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | Phosphoserine; by CaMK2 and PKA2 Publications | 1 | |
Modified residuei | 71 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q66JT0 |
PaxDbi | Q66JT0 |
PeptideAtlasi | Q66JT0 |
PRIDEi | Q66JT0 |
PTM databases
iPTMneti | Q66JT0 |
PhosphoSitePlusi | Q66JT0 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000037159, Expressed in embryo and 76 other tissues |
ExpressionAtlasi | Q66JT0, baseline and differential |
Genevisiblei | Q66JT0, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038754 |
Miscellaneous databases
RNActi | Q66JT0, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 208 – 485 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 278 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 112 | DisorderedSequence analysisAdd BLAST | 112 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 488 – 514 | Sequence analysisAdd BLAST | 27 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 167 – 169 | Nuclear localization signal | 3 | |
Motifi | 310 – 324 | Nuclear export signalAdd BLAST | 15 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 21 – 37 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 42 – 74 | Basic and acidic residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 96 – 112 | Polar residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0601, Eukaryota |
GeneTreei | ENSGT00940000158803 |
HOGENOMi | CLU_000288_25_1_1 |
InParanoidi | Q66JT0 |
OMAi | GAAWHHI |
OrthoDBi | 1063695at2759 |
PhylomeDBi | Q66JT0 |
TreeFami | TF101088 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR017164, Wee1-like_protein_kinase |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF037281, Wee1-like_protein_kinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MADTETDQGL NKKLSFSFCE EDTESEGQMT AQDIGGAQSQ KPGREESEEL
60 70 80 90 100
EGPVPPTRDE LHTSLSRDKE SPGPDLWKSV SGPPKCPETP VLHSKSKLPV
110 120 130 140 150
PTNFSTPKNS LGQPEISLLE KLSSRSSRHM RLTPASLMDE KTSLSLVNIN
160 170 180 190 200
PFTPETYRKL LLQSKGKRKT RDYLEETGEE ESKSVQWLPA KRSILQETNM
210 220 230 240 250
ASRYEKEFFE IEKIGVGEFG TVYKCIKRLD GCIYAIKRSA KSFSGLSNEL
260 270 280 290 300
DLHEVYAHAV LGHHPHVVRY YSSWIEDDHV VIQNEYCNGG SLQAAISENT
310 320 330 340 350
ASNNHFQEPK LKDILLQISL GLKYIHNSGM VHLDIKPSNI FICHKMQCDS
360 370 380 390 400
PVGPEEAESE ADWFLNASVM YKIGDLGHAT SISKPKVEEG DTRFLANEIL
410 420 430 440 450
QENYQHLPKA DIFALGLTIA VAAGAESLPI NGDMWHHIRK GNFPEISQEL
460 470 480 490 500
SDDFYGLLKN MIHPAPKERP SAAALARSRI LWPFLEKTDE LQKQLNLEKS
510 520 530 540 550
KTATLKRELK KARHIQTPQR EVHHCYYQIC KGSRHLLVGG RRKAPSSFTR
GTSSV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z4B4 | D3Z4B4_MOUSE | Wee1-like protein kinase 2 | Wee2 | 59 | Annotation score: | ||
A0A0J9YVE7 | A0A0J9YVE7_MOUSE | Wee1-like protein kinase 2 | Wee2 | 209 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 21 | E → K in AAH80784 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 70 | E → G in AAH52883 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 98 | L → P in AAH52883 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 118 | L → R in AAH80784 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 138 | M → V in AAH52883 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ011691 mRNA Translation: AAY44075.1 AK163344 mRNA Translation: BAE37310.1 AK143336 mRNA Translation: BAE25345.1 AK139573 mRNA Translation: BAE24069.1 BC052883 mRNA Translation: AAH52883.1 BC080784 mRNA Translation: AAH80784.1 |
CCDSi | CCDS20029.1 |
RefSeqi | NP_958758.2, NM_201370.2 XP_006506389.1, XM_006506326.3 |
Genome annotation databases
Ensembli | ENSMUST00000038907; ENSMUSP00000038754; ENSMUSG00000037159 |
GeneIDi | 381759 |
KEGGi | mmu:381759 |
UCSCi | uc009bmq.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ011691 mRNA Translation: AAY44075.1 AK163344 mRNA Translation: BAE37310.1 AK143336 mRNA Translation: BAE25345.1 AK139573 mRNA Translation: BAE24069.1 BC052883 mRNA Translation: AAH52883.1 BC080784 mRNA Translation: AAH80784.1 |
CCDSi | CCDS20029.1 |
RefSeqi | NP_958758.2, NM_201370.2 XP_006506389.1, XM_006506326.3 |
3D structure databases
AlphaFoldDBi | Q66JT0 |
SMRi | Q66JT0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038754 |
PTM databases
iPTMneti | Q66JT0 |
PhosphoSitePlusi | Q66JT0 |
Proteomic databases
MaxQBi | Q66JT0 |
PaxDbi | Q66JT0 |
PeptideAtlasi | Q66JT0 |
PRIDEi | Q66JT0 |
Protocols and materials databases
Antibodypediai | 32491, 111 antibodies from 24 providers |
DNASUi | 381759 |
Genome annotation databases
Ensembli | ENSMUST00000038907; ENSMUSP00000038754; ENSMUSG00000037159 |
GeneIDi | 381759 |
KEGGi | mmu:381759 |
UCSCi | uc009bmq.2, mouse |
Organism-specific databases
CTDi | 494551 |
MGIi | MGI:3027899, Wee2 |
VEuPathDBi | HostDB:ENSMUSG00000037159 |
Phylogenomic databases
eggNOGi | KOG0601, Eukaryota |
GeneTreei | ENSGT00940000158803 |
HOGENOMi | CLU_000288_25_1_1 |
InParanoidi | Q66JT0 |
OMAi | GAAWHHI |
OrthoDBi | 1063695at2759 |
PhylomeDBi | Q66JT0 |
TreeFami | TF101088 |
Miscellaneous databases
BioGRID-ORCSi | 381759, 2 hits in 73 CRISPR screens |
ChiTaRSi | Wee2, mouse |
PROi | PR:Q66JT0 |
RNActi | Q66JT0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037159, Expressed in embryo and 76 other tissues |
ExpressionAtlasi | Q66JT0, baseline and differential |
Genevisiblei | Q66JT0, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS IPR017164, Wee1-like_protein_kinase |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PIRSFi | PIRSF037281, Wee1-like_protein_kinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | WEE2_MOUSE | |
Accessioni | Q66JT0Primary (citable) accession number: Q66JT0 Secondary accession number(s): Q4U4S4, Q7TPV9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | September 5, 2006 | |
Last modified: | May 25, 2022 | |
This is version 134 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families