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Protein

Eukaryotic translation initiation factor 3 subunit J-B

Gene

Eif3j2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit J-BUniRule annotation
Short name:
eIF3j-BUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 1-BUniRule annotation
eIF-3-alpha-BUniRule annotation
eIF3 p35UniRule annotation
Gene namesi
Name:Eif3j2
Synonyms:Eif3s1-2, Gm9781
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:3704486 Eif3j2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001235072 – 263Eukaryotic translation initiation factor 3 subunit J-BAdd BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei16PhosphoserineUniRule annotationBy similarity1
Modified residuei18PhosphoserineUniRule annotationBy similarity1
Modified residuei25PhosphoserineUniRule annotationBy similarity1
Cross-linki111Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei114PhosphothreonineUniRule annotationBy similarity1
Modified residuei132PhosphoserineUniRule annotationBy similarity1
Modified residuei259PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.UniRule annotation

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ66JS6
PaxDbiQ66JS6
PeptideAtlasiQ66JS6
PRIDEiQ66JS6

PTM databases

iPTMnetiQ66JS6
PhosphoSitePlusiQ66JS6

Expressioni

Gene expression databases

BgeeiENSMUSG00000043424 Expressed in 21 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiQ66JS6 MM

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.UniRule annotation

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054421

Structurei

3D structure databases

ProteinModelPortaliQ66JS6
SMRiQ66JS6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 74Sufficient for interaction with EIF3BUniRule annotationAdd BLAST69
Regioni248 – 263Promotes stable association with the 40S ribosomeUniRule annotationAdd BLAST16

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili75 – 140UniRule annotationAdd BLAST66

Sequence similaritiesi

Belongs to the eIF-3 subunit J family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4813 Eukaryota
ENOG4111U5G LUCA
GeneTreeiENSGT00390000018400
HOGENOMiHOG000238746
HOVERGENiHBG066230
InParanoidiQ66JS6
KOiK03245
OMAiNDFDDFM
OrthoDBiEOG091G141O
PhylomeDBiQ66JS6
TreeFamiTF101514

Family and domain databases

Gene3Di1.10.246.60, 1 hit
HAMAPiMF_03009 eIF3j, 1 hit
InterProiView protein in InterPro
IPR023194 eIF3-like_dom_sf
IPR013906 eIF3j
PANTHERiPTHR21681 PTHR21681, 1 hit
PfamiView protein in Pfam
PF08597 eIF3_subunit, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66JS6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAAAAAAA AAAGDSDSWD ADTFSMEDPV RKVAGGGTAG GDRWEGEDED
60 70 80 90 100
EDVKDNWDDD DDENKEEAEV KPEVKISEKK KIAEKIKEKE RQQKKRQEEI
110 120 130 140 150
KKRLEEPEES KVLTPEEQLA DKLRLKKLQE ESDLELAKET FGVNNTVYGI
160 170 180 190 200
DAMNPSSRDD FTEFGKLLKD KITQYEKSLY YASFLEALVR DVCISLEIDD
210 220 230 240 250
LKKITNSLTV LCSEKQKQEK QSKAKKKKKG VVPGGGLKAT MKDDLADYGG
260
YEGGYVQDYE DFM
Length:263
Mass (Da):29,486
Last modified:October 11, 2004 - v1
Checksum:i7040416E40650960
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti191D → N in BAC38399 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC080788 mRNA Translation: AAH80788.1
AK082048 mRNA Translation: BAC38399.1
CCDSiCCDS57122.1
RefSeqiNP_001242984.1, NM_001256055.1
UniGeneiMm.329403
Mm.458184

Genome annotation databases

EnsembliENSMUST00000057110; ENSMUSP00000054421; ENSMUSG00000043424
GeneIDi100042807
KEGGimmu:100042807
UCSCiuc008mab.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC080788 mRNA Translation: AAH80788.1
AK082048 mRNA Translation: BAC38399.1
CCDSiCCDS57122.1
RefSeqiNP_001242984.1, NM_001256055.1
UniGeneiMm.329403
Mm.458184

3D structure databases

ProteinModelPortaliQ66JS6
SMRiQ66JS6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054421

PTM databases

iPTMnetiQ66JS6
PhosphoSitePlusiQ66JS6

Proteomic databases

MaxQBiQ66JS6
PaxDbiQ66JS6
PeptideAtlasiQ66JS6
PRIDEiQ66JS6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057110; ENSMUSP00000054421; ENSMUSG00000043424
GeneIDi100042807
KEGGimmu:100042807
UCSCiuc008mab.3 mouse

Organism-specific databases

CTDi100042807
MGIiMGI:3704486 Eif3j2

Phylogenomic databases

eggNOGiKOG4813 Eukaryota
ENOG4111U5G LUCA
GeneTreeiENSGT00390000018400
HOGENOMiHOG000238746
HOVERGENiHBG066230
InParanoidiQ66JS6
KOiK03245
OMAiNDFDDFM
OrthoDBiEOG091G141O
PhylomeDBiQ66JS6
TreeFamiTF101514

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Miscellaneous databases

PROiPR:Q66JS6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000043424 Expressed in 21 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiQ66JS6 MM

Family and domain databases

Gene3Di1.10.246.60, 1 hit
HAMAPiMF_03009 eIF3j, 1 hit
InterProiView protein in InterPro
IPR023194 eIF3-like_dom_sf
IPR013906 eIF3j
PANTHERiPTHR21681 PTHR21681, 1 hit
PfamiView protein in Pfam
PF08597 eIF3_subunit, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEI3JB_MOUSE
AccessioniPrimary (citable) accession number: Q66JS6
Secondary accession number(s): Q8BUW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 11, 2004
Last modified: November 7, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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