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Protein

Kinetochore scaffold 1

Gene

Knl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Directly links spindle checkpoint proteins BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore scaffold 1
Alternative name(s):
Cancer susceptibility candidate gene 5 protein homolog
Kinetochore-null protein 1
Protein CASC5
Gene namesi
Name:Knl1Imported
Synonyms:Casc5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1923714 Knl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002359831 – 1612Kinetochore scaffold 1Add BLAST1612

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32PhosphoserineBy similarity1
Modified residuei60PhosphoserineBy similarity1
Modified residuei538PhosphoserineBy similarity1
Modified residuei540PhosphothreonineBy similarity1
Modified residuei794PhosphoserineCombined sources1
Modified residuei878PhosphoserineBy similarity1
Modified residuei1243PhosphoserineBy similarity1
Modified residuei1464PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ66JQ7
MaxQBiQ66JQ7
PaxDbiQ66JQ7
PRIDEiQ66JQ7

PTM databases

iPTMnetiQ66JQ7
PhosphoSitePlusiQ66JQ7

Expressioni

Gene expression databases

BgeeiENSMUSG00000027326 Expressed in 128 organ(s), highest expression level in bone marrow
CleanExiMM_CASC5
ExpressionAtlasiQ66JQ7 baseline and differential
GenevisibleiQ66JQ7 MM

Interactioni

Subunit structurei

Interacts with DSN1, MIS12, BUB1, BUB1B, NSL1 and ZWINT.By similarity

Protein-protein interaction databases

IntActiQ66JQ7, 15 interactors
MINTiQ66JQ7
STRINGi10090.ENSMUSP00000028802

Structurei

3D structure databases

ProteinModelPortaliQ66JQ7
SMRiQ66JQ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati723 – 8271Add BLAST105
Repeati923 – 10272Add BLAST105

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni723 – 10272 X 104 AA approximate repeatsAdd BLAST305

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1577 – 1590Nuclear localization signalSequence analysisAdd BLAST14

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF9C Eukaryota
ENOG410XZXJ LUCA
GeneTreeiENSGT00410000025918
HOGENOMiHOG000246954
HOVERGENiHBG080986
InParanoidiQ66JQ7

Family and domain databases

InterProiView protein in InterPro
IPR037388 Blinkin
PANTHERiPTHR16520 PTHR16520, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q66JQ7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDGVYSEANE ENDNTQRPVR RQHSSILKPP RSPLQDLKCG NQTNQEPNPP
60 70 80 90 100
RKRKSSRRVS FADTIKVFQT ESHMKTERNS EISGMNTLLC APIQTQMQQK
110 120 130 140 150
EFSITDCNHE RKHANDQTVI FSDENQMDLT ASHTVMITKG LSDCTKNENS
160 170 180 190 200
TKIDTTSFLE NLKHHAANSR IKKDLACSTV SLSQNIFSEK INSDNFIKRL
210 220 230 240 250
KTGKHISSST ELDKENAEIP VYSKDSNSAS STYQMHASLG VDENSSNRTR
260 270 280 290 300
IFREQDDGMN LTQCHTACIK TWIPPSTEAK IGEFKGDKTI YGNECMELTT
310 320 330 340 350
NYTIQVLSSE NNLSERETQT QNGMNVTTVD GATAPAPEKK TALKDKLNAA
360 370 380 390 400
FQGSFPNPEN KIHIIKCHPI ESETHTVTQI SSQSASTLAV TSKSICSSPA
410 420 430 440 450
IEGYKTIFHS SSNDAMELTK CLSAMEEEKK LLKADDKYSK ICTNPDAGPL
460 470 480 490 500
REKTIYLEED SMDITKSHTV AIDNKIFKHD QENIKKEIIA IPIFEKEMVL
510 520 530 540 550
RNLMPMSKDE KRDVNYISVP QVSKESLQRS QTNTLSVSLT DKKMEFLADE
560 570 580 590 600
DMDLTKSHTT KLSQVIPTTF DLASKNVTKS YSHSKSPLNE WESLDKQVVL
610 620 630 640 650
GQHSKLPLPQ RKDRDDPDCS HHKIMYSEEL QTMDLTKSHT IVIGFGPSEV
660 670 680 690 700
QEHSKINLEH KNSQLTAESI QTAVNVPAAN SRVVTTNDMD MLKDRSTHKP
710 720 730 740 750
ELLKEKQNIK IYGRKSIGRL KIDKTILFSE GNEGDMDITK SCTVKINHRS
760 770 780 790 800
LLDKHDSHLV SLAGTSKTIL HARGQVEMEI NRSHTTPLEC KIISPSDITP
810 820 830 840 850
GDLDKTMMSI DDHEELDMTK SHTVFIDYQA EAKGVLPDRL DFQLSKKESL
860 870 880 890 900
QKPKVTSLAE EIYISKNSES NHLPAKGSQL TILEEGSNSG LGEETNDAQK
910 920 930 940 950
PGFLNELLSG KTQRRKSLSL KNKSITFPEN DKSYREIPQS SAVEINNETR
960 970 980 990 1000
LEDRKGFSFV PLAGTSKPVL SAYGPEDMEI SIGQTTASEY KTVPPEEITT
1010 1020 1030 1040 1050
IPMDKTVMFV DNFGDLDVTR SHTVFIDCQA KEKVLDEYTN LGIQKTKTLS
1060 1070 1080 1090 1100
GSEGDTHIQE ITKNPAAQHK HHMTTVIPSS TVVSDQSSMK IKFHKADRDE
1110 1120 1130 1140 1150
EVKGKEVEAN MLKQTKPESC LLNITDGKNV DFTSSYTADV CRSSDKYSSL
1160 1170 1180 1190 1200
PNISSSDNSG GNTMSLCDKN KEKAYNCQVP NEFTYAAILP STYHMDSKKL
1210 1220 1230 1240 1250
SVFPPCPSKE VTQTESAIAL LKDEDPVEEP LGEMATFNSK HVSLNLAKDQ
1260 1270 1280 1290 1300
TEAFVDVSVA SQPHLSAQQS PSTQKGQDVA RRDEGILAKA GKKALPFLLE
1310 1320 1330 1340 1350
NVAASTWENE SKIPTNVEHF AVTYEKELSI SIQTDKCNTN VQSPSNSALT
1360 1370 1380 1390 1400
TQVIQTHANA EGALDFLVPS TVSCFSSTKP SLSNLNRKTE EVLDFQTVNL
1410 1420 1430 1440 1450
LPPAEQLLEE GSQAHSMSIV QATEIYRLGS RNDRDEESKT FCNEAETTSV
1460 1470 1480 1490 1500
PLKTAVKDKT RRCSLGIFLP KLPSKRSCSI TGVDDLEQIL ADAADLTQLE
1510 1520 1530 1540 1550
TQPVCSKDPG IGSVAAKLNL SPSQFINEEN LPVYPGEILS SDSVSLDIEE
1560 1570 1580 1590 1600
SVLIDTSQRE SLPSENKTEN CRAQKRTRVE ENDVTNEKKI RTHDSAQDQE
1610
VSCLEPKNVL EF
Length:1,612
Mass (Da):179,337
Last modified:July 27, 2011 - v3
Checksum:iA9BE2BB20E008621
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A3KGI3A3KGI3_MOUSE
Kinetochore scaffold 1
Knl1
2,119Annotation score:
A3KGI5A3KGI5_MOUSE
Kinetochore scaffold 1
Knl1
484Annotation score:
A3KGI6A3KGI6_MOUSE
Kinetochore scaffold 1
Knl1
91Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128D → G in AAH80815 (PubMed:15489334).Curated1
Sequence conflicti375H → R in AAH80815 (PubMed:15489334).Curated1
Sequence conflicti1151P → L in AAH80815 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL772264 Genomic DNA No translation available.
BC080815 mRNA Translation: AAH80815.1
AK009772 mRNA Translation: BAB26494.1
AK033132 mRNA Translation: BAC28167.2
UniGeneiMm.202518
Mm.392877

Genome annotation databases

EnsembliENSMUST00000028799; ENSMUSP00000028799; ENSMUSG00000027326
UCSCiuc008ltb.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL772264 Genomic DNA No translation available.
BC080815 mRNA Translation: AAH80815.1
AK009772 mRNA Translation: BAB26494.1
AK033132 mRNA Translation: BAC28167.2
UniGeneiMm.202518
Mm.392877

3D structure databases

ProteinModelPortaliQ66JQ7
SMRiQ66JQ7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ66JQ7, 15 interactors
MINTiQ66JQ7
STRINGi10090.ENSMUSP00000028802

PTM databases

iPTMnetiQ66JQ7
PhosphoSitePlusiQ66JQ7

Proteomic databases

EPDiQ66JQ7
MaxQBiQ66JQ7
PaxDbiQ66JQ7
PRIDEiQ66JQ7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028799; ENSMUSP00000028799; ENSMUSG00000027326
UCSCiuc008ltb.1 mouse

Organism-specific databases

MGIiMGI:1923714 Knl1

Phylogenomic databases

eggNOGiENOG410IF9C Eukaryota
ENOG410XZXJ LUCA
GeneTreeiENSGT00410000025918
HOGENOMiHOG000246954
HOVERGENiHBG080986
InParanoidiQ66JQ7

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-MMU-68877 Mitotic Prometaphase

Miscellaneous databases

PROiPR:Q66JQ7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027326 Expressed in 128 organ(s), highest expression level in bone marrow
CleanExiMM_CASC5
ExpressionAtlasiQ66JQ7 baseline and differential
GenevisibleiQ66JQ7 MM

Family and domain databases

InterProiView protein in InterPro
IPR037388 Blinkin
PANTHERiPTHR16520 PTHR16520, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKNL1_MOUSE
AccessioniPrimary (citable) accession number: Q66JQ7
Secondary accession number(s): A3KGI4, Q8CCH6, Q9CV38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 102 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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