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Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Gene

Ndufs1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi75Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi78Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi92Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi124Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi128Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi131Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi137Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi176Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi179Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi182Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi226Iron-sulfur 3 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-RNO-611105 Respiratory electron transport
R-RNO-6799198 Complex I biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Gene namesi
Name:Ndufs1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi1359670 Ndufs1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 23MitochondrionBy similarityAdd BLAST23
ChainiPRO_000027138824 – 727NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialAdd BLAST704

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei84N6-acetyllysineBy similarity1
Modified residuei499N6-acetyllysineBy similarity1
Modified residuei709N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ66HF1
PRIDEiQ66HF1

PTM databases

CarbonylDBiQ66HF1
iPTMnetiQ66HF1
PhosphoSitePlusiQ66HF1

Expressioni

Gene expression databases

BgeeiENSRNOG00000011849 Expressed in 10 organ(s), highest expression level in heart
GenevisibleiQ66HF1 RN

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.By similarity

Protein-protein interaction databases

BioGridi256969, 2 interactors
STRINGi10116.ENSRNOP00000015851

Structurei

3D structure databases

ProteinModelPortaliQ66HF1
SMRiQ66HF1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 1082Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST79
Domaini108 – 1474Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini245 – 3014Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2282 Eukaryota
COG1034 LUCA
GeneTreeiENSGT00390000018768
HOGENOMiHOG000031442
HOVERGENiHBG003482
InParanoidiQ66HF1
KOiK03934
OMAiPQASCAM
OrthoDBiEOG091G02C2
PhylomeDBiQ66HF1
TreeFamiTF105756

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
IPR015405 NuoG_C
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
PF09326 NADH_dhqG_C, 1 hit
SMARTiView protein in SMART
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q66HF1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRIPVKRAL IGLSKSPKGY VRSTGTAASN LIEVFVDGQS VMVEPGTTVL
60 70 80 90 100
QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVVA ACAMPVMKGW
110 120 130 140 150
NILTNSEKSK KAREGVMEFL LANHPLDCPI CDQGGECDLQ DQSMMFGSDR
160 170 180 190 200
SRFLEGKRAV EDKNIGPLVK TIMTRCIQCT RCIRFASEIA GVDDLGTTGR
210 220 230 240 250
GNDMQVGTYI EKMFMSELSG NIIDICPVGA LTSKPYAFTA RPWETRKTES
260 270 280 290 300
IDVMDAVGSN IVVSTRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ
310 320 330 340 350
RLTEPMVRNE KGLLTYTSWE DALSRVAGML QSFEGKAVAA IAGGLVDAEA
360 370 380 390 400
LVALKDLLNK VDSDTLCTEE IFPNEGAGTD LRSNYLLNTT IAGVEEADVV
410 420 430 440 450
LLVGTNPRFE APLFNARIRK SWLHNDLKVA LIGSPVDLTY RYDHLGDSPK
460 470 480 490 500
ILQDIASGNH EFSKVLNAAK KPMVVLGSSA LQRDDGAAIL AAVSSIAQKI
510 520 530 540 550
RVASGAAAEW KVMNILHRIA SQVAALDLGY KPGVEAIRKN PPKLLFLLGA
560 570 580 590 600
DGGCITRQDL PKDCFIVYQG HHGDVGAPIA DVILPGAAYT EKSATYVNTE
610 620 630 640 650
GRAQQTKVAV TPPGLAREDW KIIRALSEIA GITLPYDTLD QVRNRLGEVS
660 670 680 690 700
PNLVRYDDVE EANYFQQASE LAKLVDQEFL ADPLVPPQLT IKDFYMTDSI
710 720
SRASQTMAKC VKAVTEGAQA VEEPSIC
Length:727
Mass (Da):79,412
Last modified:October 11, 2004 - v1
Checksum:iEDAC634B2697984A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC081892 mRNA Translation: AAH81892.1
RefSeqiNP_001005550.1, NM_001005550.1
UniGeneiRn.1467

Genome annotation databases

EnsembliENSRNOT00000015852; ENSRNOP00000015851; ENSRNOG00000011849
GeneIDi301458
KEGGirno:301458
UCSCiRGD:1359670 rat

Similar proteinsi

Entry informationi

Entry nameiNDUS1_RAT
AccessioniPrimary (citable) accession number: Q66HF1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: October 11, 2004
Last modified: September 12, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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