UniProtKB - Q66HF1 (NDUS1_RAT)
Protein
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
Gene
Ndufs1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (By similarity). Essential for catalysing the entry and efficient transfer of electrons within complex I (By similarity). Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes (By similarity).By similarity
Catalytic activityi
- EC:7.1.1.2By similarity
Cofactori
Protein has several cofactor binding sites:- [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
- [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 64 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 75 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 78 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 92 | Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 124 | Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 128 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 131 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 137 | Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 176 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 179 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 182 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 226 | Iron-sulfur 3 (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4 iron, 4 sulfur cluster binding Source: GO_Central
- electron transfer activity Source: RGD
- metal ion binding Source: UniProtKB-KW
- NADH dehydrogenase (ubiquinone) activity Source: UniProtKB
GO - Biological processi
- apoptotic mitochondrial changes Source: UniProtKB
- ATP metabolic process Source: UniProtKB
- cellular respiration Source: UniProtKB
- mitochondrial electron transport, NADH to ubiquinone Source: UniProtKB
- mitochondrial respiratory chain complex I assembly Source: UniProtKB
- reactive oxygen species metabolic process Source: UniProtKB
- regulation of mitochondrial membrane potential Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase, Translocase |
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | 2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Enzyme and pathway databases
Reactomei | R-RNO-611105, Respiratory electron transport R-RNO-6799198, Complex I biogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (EC:7.1.1.2By similarity) |
Gene namesi | Name:Ndufs1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1359670, Ndufs1 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity
Mitochondrion
- mitochondrial intermembrane space Source: UniProtKB
- mitochondrial respiratory chain complex I Source: UniProtKB
- mitochondrion Source: UniProtKB
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 23 | MitochondrionBy similarityAdd BLAST | 23 | |
ChainiPRO_0000271388 | 24 – 727 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialAdd BLAST | 704 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 84 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 499 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 709 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | Q66HF1 |
PaxDbi | Q66HF1 |
PRIDEi | Q66HF1 |
PTM databases
CarbonylDBi | Q66HF1 |
iPTMneti | Q66HF1 |
PhosphoSitePlusi | Q66HF1 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000011849, Expressed in heart and 21 other tissues |
Genevisiblei | Q66HF1, RN |
Interactioni
Subunit structurei
Core subunit of respiratory chain NADH dehydrogenase (Complex I) which is composed of 45 different subunits (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme (By similarity). Complex I associates with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes (By similarity).
Interacts with MDM2 and AKAP1 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 256969, 4 interactors |
IntActi | Q66HF1, 3 interactors |
STRINGi | 10116.ENSRNOP00000015851 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 108 | 2Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 108 – 147 | 4Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 245 – 301 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 57 |
Sequence similaritiesi
Belongs to the complex I 75 kDa subunit family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2282, Eukaryota |
GeneTreei | ENSGT00940000153514 |
HOGENOMi | CLU_000422_11_6_1 |
InParanoidi | Q66HF1 |
OMAi | QDQAMAY |
OrthoDBi | 1095510at2759 |
PhylomeDBi | Q66HF1 |
TreeFami | TF105756 |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd IPR015405, NuoG_C |
Pfami | View protein in Pfam PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit PF09326, NADH_dhqG_C, 1 hit |
SMARTi | View protein in SMART SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q66HF1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRIPVKRAL IGLSKSPKGY VRSTGTAASN LIEVFVDGQS VMVEPGTTVL
60 70 80 90 100
QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVVA ACAMPVMKGW
110 120 130 140 150
NILTNSEKSK KAREGVMEFL LANHPLDCPI CDQGGECDLQ DQSMMFGSDR
160 170 180 190 200
SRFLEGKRAV EDKNIGPLVK TIMTRCIQCT RCIRFASEIA GVDDLGTTGR
210 220 230 240 250
GNDMQVGTYI EKMFMSELSG NIIDICPVGA LTSKPYAFTA RPWETRKTES
260 270 280 290 300
IDVMDAVGSN IVVSTRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ
310 320 330 340 350
RLTEPMVRNE KGLLTYTSWE DALSRVAGML QSFEGKAVAA IAGGLVDAEA
360 370 380 390 400
LVALKDLLNK VDSDTLCTEE IFPNEGAGTD LRSNYLLNTT IAGVEEADVV
410 420 430 440 450
LLVGTNPRFE APLFNARIRK SWLHNDLKVA LIGSPVDLTY RYDHLGDSPK
460 470 480 490 500
ILQDIASGNH EFSKVLNAAK KPMVVLGSSA LQRDDGAAIL AAVSSIAQKI
510 520 530 540 550
RVASGAAAEW KVMNILHRIA SQVAALDLGY KPGVEAIRKN PPKLLFLLGA
560 570 580 590 600
DGGCITRQDL PKDCFIVYQG HHGDVGAPIA DVILPGAAYT EKSATYVNTE
610 620 630 640 650
GRAQQTKVAV TPPGLAREDW KIIRALSEIA GITLPYDTLD QVRNRLGEVS
660 670 680 690 700
PNLVRYDDVE EANYFQQASE LAKLVDQEFL ADPLVPPQLT IKDFYMTDSI
710 720
SRASQTMAKC VKAVTEGAQA VEEPSIC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC081892 mRNA Translation: AAH81892.1 |
RefSeqi | NP_001005550.1, NM_001005550.1 |
Genome annotation databases
Ensembli | ENSRNOT00000015852; ENSRNOP00000015851; ENSRNOG00000011849 |
GeneIDi | 301458 |
KEGGi | rno:301458 |
UCSCi | RGD:1359670, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC081892 mRNA Translation: AAH81892.1 |
RefSeqi | NP_001005550.1, NM_001005550.1 |
3D structure databases
SMRi | Q66HF1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 256969, 4 interactors |
IntActi | Q66HF1, 3 interactors |
STRINGi | 10116.ENSRNOP00000015851 |
PTM databases
CarbonylDBi | Q66HF1 |
iPTMneti | Q66HF1 |
PhosphoSitePlusi | Q66HF1 |
Proteomic databases
jPOSTi | Q66HF1 |
PaxDbi | Q66HF1 |
PRIDEi | Q66HF1 |
Genome annotation databases
Ensembli | ENSRNOT00000015852; ENSRNOP00000015851; ENSRNOG00000011849 |
GeneIDi | 301458 |
KEGGi | rno:301458 |
UCSCi | RGD:1359670, rat |
Organism-specific databases
CTDi | 4719 |
RGDi | 1359670, Ndufs1 |
Phylogenomic databases
eggNOGi | KOG2282, Eukaryota |
GeneTreei | ENSGT00940000153514 |
HOGENOMi | CLU_000422_11_6_1 |
InParanoidi | Q66HF1 |
OMAi | QDQAMAY |
OrthoDBi | 1095510at2759 |
PhylomeDBi | Q66HF1 |
TreeFami | TF105756 |
Enzyme and pathway databases
Reactomei | R-RNO-611105, Respiratory electron transport R-RNO-6799198, Complex I biogenesis |
Miscellaneous databases
PROi | PR:Q66HF1 |
Gene expression databases
Bgeei | ENSRNOG00000011849, Expressed in heart and 21 other tissues |
Genevisiblei | Q66HF1, RN |
Family and domain databases
CDDi | cd00207, fer2, 1 hit |
InterProi | View protein in InterPro IPR036010, 2Fe-2S_ferredoxin-like_sf IPR001041, 2Fe-2S_ferredoxin-type IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR000283, NADH_UbQ_OxRdtase_75kDa_su_CS IPR010228, NADH_UbQ_OxRdtase_Gsu IPR019574, NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd IPR015405, NuoG_C |
Pfami | View protein in Pfam PF00384, Molybdopterin, 1 hit PF10588, NADH-G_4Fe-4S_3, 1 hit PF09326, NADH_dhqG_C, 1 hit |
SMARTi | View protein in SMART SM00929, NADH-G_4Fe-4S_3, 1 hit |
SUPFAMi | SSF54292, SSF54292, 1 hit |
TIGRFAMsi | TIGR01973, NuoG, 1 hit |
PROSITEi | View protein in PROSITE PS51085, 2FE2S_FER_2, 1 hit PS51839, 4FE4S_HC3, 1 hit PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00641, COMPLEX1_75K_1, 1 hit PS00642, COMPLEX1_75K_2, 1 hit PS00643, COMPLEX1_75K_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NDUS1_RAT | |
Accessioni | Q66HF1Primary (citable) accession number: Q66HF1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 9, 2007 |
Last sequence update: | October 11, 2004 | |
Last modified: | April 7, 2021 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families